Restriction map for
pF1KSDB0052
(Circular) (Six-base) MAPSORT of: pF1KSDB0052.seq Check: 7870 from: 1 to: 5002
pF1KSDB0052 5002 bp
With 182 enzymes: SgfI *
June 20, 2008 10:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1088 1088
Size: 5002
AatII G_ACGT'C
Cuts at: 1973 1973
Size: 5002
AccI GT'mk_AC
Cuts at: 2038 2038
Size: 5002
Acc65I G'GTAC_C
Cuts at: 2016 2016
Size: 5002
AclI AA'CG_TT
Cuts at: 4842 4842
Size: 5002
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 487 928 1123 1348 2825 3257 4006 4274 487
Size: 441 195 225 1477 432 749 268 1215
Fragments arranged by size:
1477 1215 749 441 432 268 225 195
AflIII A'CryG_T
Cuts at: 2352 3458 4435 2352
Size: 1106 977 2919
Fragments arranged by size:
2919 1106 977
AgeI A'CCGG_T
Cuts at: 3390 3390
Size: 5002
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1407 1882 1407
Size: 475 4527
AlwNI CAG_nnn'CTG
Cuts at: 851 1009 1210 1767 1962 2305 3874 851
Size: 158 201 557 195 343 1569 1979
Fragments arranged by size:
1979 1569 557 343 201 195 158
ApaLI G'TGCA_C
Cuts at: 3772 3772
Size: 5002
ApoI r'AATT_y
Cuts at: 950 2004 4355 4611 950
Size: 1054 2351 256 1341
Fragments arranged by size:
2351 1341 1054 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5002
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5002
AvaI C'yCGr_G
Cuts at: 1893 2020 1893
Size: 127 4875
BamHI G'GATC_C
Cuts at: 2025 2025
Size: 5002
BanI G'GyrC_C
Cuts at: 418 1076 1794 2016 2430 2678 2713 418
Size: 658 718 222 414 248 35 2707
Fragments arranged by size:
2707 718 658 414 248 222 35
BanII G_rGCy'C
Cuts at: 233 536 2014 3044 233
Size: 303 1478 1030 2191
Fragments arranged by size:
2191 1478 1030 303
BbeI G_GCGC'C
Cuts at: 2434 2682 2434
Size: 248 4754
BbsI GAAGACnn'nnnn_
Cuts at: 1336 1336
Size: 5002
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 400 434 400
Size: 34 4968
BciVI GTATCCnnnnn_n'
Cuts at: 2898 3661 5002 2898
Size: 763 1341 2898
Fragments arranged by size:
2898 1341 763
BclI T'GATC_A
Cuts at: 2520 2520
Size: 5002
BglII A'GATC_T
Cuts at: 2515 4554 2515
Size: 2039 2963
BlpI GC'TnA_GC
Cuts at: 2112 2112
Size: 5002
Bme1580I G_kGCm'C
Cuts at: 1079 1797 2625 2718 3776 1079
Size: 718 828 93 1058 2305
Fragments arranged by size:
2305 1058 828 718 93
BmgBI CAC'GTC
Cuts at: 1662 1662
Size: 5002
BmrI ACTGGGnnnn_n'
Cuts at: 390 1160 1710 2212 2293 2628 4774 390
Size: 770 550 502 81 335 2146 618
Fragments arranged by size:
2146 770 618 550 502 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 471 3367 471
Size: 2896 2106
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 245 257 466 529 1166 1589 1773 1820 245
Size: 12 209 63 637 423 184 47 3427
Fragments arranged by size:
3427 637 423 209 184 63 47 12
Bpu10I CC'TnA_GC
Cuts at: 1379 1379
Size: 5002
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 344 2176 3055 3549 3847 344
Size: 1832 879 494 298 1499
Fragments arranged by size:
1832 1499 879 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 370 539 850 4706 370
Size: 169 311 3856 666
Fragments arranged by size:
3856 666 311 169
BsaAI yAC'GTr
Cuts at: 1992 2353 1992
Size: 361 4641
BsaBI GATnn'nnATC
Cuts at: 6 1495 2514 4553 6
Size: 1489 1019 2039 455
Fragments arranged by size:
2039 1489 1019 455
BsaHI Gr'CG_yC
Cuts at: 1889 1970 2431 2679 3383 4879 1889
Size: 81 461 248 704 1496 2012
Fragments arranged by size:
2012 1496 704 461 248 81
BsaWI w'CCGG_w
Cuts at: 1880 2189 2710 3390 3664 3811 4222 1880
Size: 309 521 680 274 147 411 2660
Fragments arranged by size:
2660 680 521 411 309 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 526 1638 526
Size: 1112 3890
BseYI C'CCAG_C
Cuts at: 321 549 1854 2425 3183 3762 4258 321
Size: 228 1305 571 758 579 496 1065
Fragments arranged by size:
1305 1065 758 579 571 496 228
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 408 873 970 1888 408
Size: 465 97 918 3522
Fragments arranged by size:
3522 918 465 97
BsiEI CG_ry'CG
Cuts at: 104 2542 2588 3798 104
Size: 2438 46 1210 1308
Fragments arranged by size:
2438 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 233 1142 2014 2792 2982 3776 233
Size: 909 872 778 190 794 1459
Fragments arranged by size:
1459 909 872 794 778 190
BsmI GAATG_Cn'
Cuts at: 1384 1384
Size: 5002
BsmBI CGTCTCn'nnnn_
Cuts at: 1896 1896
Size: 5002
Bsp1286I G_dGCh'C
Cuts at: 233 536 1079 1142 1797 2014 2625 2718 2792
Size: 303 543 63 655 217 611 93 74
Cuts at: 2792 2982 3044 3776 233
Size: 190 62 732 1459
Fragments arranged by size:
1459 732 655 611 543 303 217 190
93 74 63 62
BspEI T'CCGG_A
Cuts at: 1880 4222 1880
Size: 2342 2660
BspHI T'CATG_A
Cuts at: 1173 4178 1173
Size: 3005 1997
BspMI ACCTGCnnnn'nnnn_
Cuts at: 544 771 1088 2050 2264 2566 2947 544
Size: 227 317 962 214 302 381 2599
Fragments arranged by size:
2599 962 381 317 302 227 214
BsrBI CCG'CTC
Cuts at: 50 1133 3292 3346 4832 4997 50
Size: 1083 2159 54 1486 165 55
Fragments arranged by size:
2159 1486 1083 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1682 2912 1682
Size: 1230 3772
BsrFI r'CCGG_y
Cuts at: 427 2998 3179 3390 427
Size: 2571 181 211 2039
Fragments arranged by size:
2571 2039 211 181
BssSI C'ACGA_G
Cuts at: 3271 3631 3271
Size: 360 4642
BstAPI GCAn_nnn'nTGC
Cuts at: 2377 2377
Size: 5002
BstBI TT'CG_AA
Cuts at: 110 110
Size: 5002
BstXI CCAn_nnnn'nTGG
Cuts at: 249 344 812 249
Size: 95 468 4439
Fragments arranged by size:
4439 468 95
BstYI r'GATC_y
Cuts at: 1 978 1337 2025 2492 2515 2850 3096 4110
Size: 977 359 688 467 23 335 246 1014
Cuts at: 4110 4196 4208 4554 1
Size: 86 12 346 449
Fragments arranged by size:
1014 977 688 467 449 359 346 335
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 145 1768 145
Size: 1623 3379
BtgI C'CryG_G
Cuts at: 779 1976 779
Size: 1197 3805
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 981 1635 2950 3131 3294 981
Size: 654 1315 181 163 2689
Fragments arranged by size:
2689 1315 654 181 163
BtsI GCAGTG_nn'
Cuts at: 2243 2243
Size: 5002
DraI TTT'AAA
Cuts at: 1999 1999
Size: 5002
DraIII CAC_nnn'GTG
Cuts at: 1434 1434
Size: 5002
DrdI GACnn_nn'nnGTC
Cuts at: 1678 2706 3566 1678
Size: 1028 860 3114
Fragments arranged by size:
3114 1028 860
EaeI y'GGCC_r
Cuts at: 1436 2585 2759 3150 3177 4524 1436
Size: 1149 174 391 27 1347 1914
Fragments arranged by size:
1914 1347 1149 391 174 27
EagI C'GGCC_G
Cuts at: 2585 2585
Size: 5002
EarI CTCTTCn'nnn_
Cuts at: 537 1181 1449 3023 3233 537
Size: 644 268 1574 210 2306
Fragments arranged by size:
2306 1574 644 268 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3208 3520 3666 4812 3208
Size: 312 146 1146 3398
Fragments arranged by size:
3398 1146 312 146
EcoICRI GAG'CTC
Cuts at: 231 2012 231
Size: 1781 3221
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 245 257 466 487 529 928 1123 1166 1348
Size: 12 209 21 42 399 195 43 182
Cuts at: 1348 1589 1773 1820 2825 3257 4006 4274 245
Size: 241 184 47 1005 432 749 268 973
Fragments arranged by size:
1005 973 749 432 399 268 241 209
195 184 182 47 43 42 21 12
EcoNI CCTnn'n_nnAGG
Cuts at: 511 511
Size: 5002
EcoO109I rG'GnC_Cy
Cuts at: 149 263 1404 1821 2139 149
Size: 114 1141 417 318 3012
Fragments arranged by size:
3012 1141 417 318 114
EcoRI G'AATT_C
Cuts at: 950 2004 950
Size: 1054 3948
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3019 3019
Size: 5002
FspI TGC'GCA
Cuts at: 1725 2781 1725
Size: 1056 3946
HaeII r_GCGC'y
Cuts at: 2434 2682 3706 4685 2434
Size: 248 1024 979 2751
Fragments arranged by size:
2751 1024 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 375 407 2842 2872 2874 2904 375
Size: 32 2435 30 2 30 2473
Fragments arranged by size:
2473 2435 32 30 30 2
HincII GTy'rAC
Cuts at: 300 1266 1512 2039 300
Size: 966 246 527 3263
Fragments arranged by size:
3263 966 527 246
KasI G'GCGC_C
Cuts at: 2430 2678 2430
Size: 248 4754
KpnI G_GTAC'C
Cuts at: 2020 2020
Size: 5002
MluI A'CGCG_T
Cuts at: 4435 4435
Size: 5002
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 258 1761 3673 3857 258
Size: 1503 1912 184 1403
Fragments arranged by size:
1912 1503 1403 184
MscI TGG'CCA
Cuts at: 1438 2761 1438
Size: 1323 3679
MslI CAynn'nnrTG
Cuts at: 895 1389 1643 1864 1912 2989 3116 895
Size: 494 254 221 48 1077 127 2781
Fragments arranged by size:
2781 1077 494 254 221 127 48
MspA1I CmG'CkG
Cuts at: 465 825 2111 2425 2785 3800 4045 465
Size: 360 1286 314 360 1015 245 1422
Fragments arranged by size:
1422 1286 1015 360 360 314 245
NaeI GCC'GGC
Cuts at: 3181 3181
Size: 5002
NarI GG'CG_CC
Cuts at: 2431 2679 2431
Size: 248 4754
NcoI C'CATG_G
Cuts at: 779 779
Size: 5002
NgoMIV G'CCGG_C
Cuts at: 3179 3179
Size: 5002
NruI TCG'CGA
Cuts at: 3363 3363
Size: 5002
NspI r_CATG'y
Cuts at: 2053 2553 3462 2053
Size: 500 909 3593
Fragments arranged by size:
3593 909 500
PasI CC'CwG_GG
Cuts at: 793 1057 793
Size: 264 4738
PciI A'CATG_T
Cuts at: 3458 3458
Size: 5002
PflMI CCAn_nnn'nTGG
Cuts at: 1434 1962 1434
Size: 528 4474
PfoI T'CCnGG_A
Cuts at: 1551 1551
Size: 5002
PmeI GTTT'AAAC
Cuts at: 1999 1999
Size: 5002
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4141 4173 4141
Size: 32 4970
PpuMI rG'GwC_Cy
Cuts at: 149 263 1404 149
Size: 114 1141 3747
Fragments arranged by size:
3747 1141 114
PstI C_TGCA'G
Cuts at: 499 1235 2047 2732 499
Size: 736 812 685 2769
Fragments arranged by size:
2769 812 736 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5002
PvuII CAG'CTG
Cuts at: 465 2425 2785 465
Size: 1960 360 2682
Fragments arranged by size:
2682 1960 360
RsrII CG'GwC_CG
Cuts at: 3195 3195
Size: 5002
SacI G_AGCT'C
Cuts at: 233 2014 233
Size: 1781 3221
SalI G'TCGA_C
Cuts at: 2037 2037
Size: 5002
SanDI GG'GwC_CC
Cuts at: 1404 1404
Size: 5002
SapI GCTCTTCn'nnn_
Cuts at: 1449 3023 3233 1449
Size: 1574 210 3218
Fragments arranged by size:
3218 1574 210
SbfI CC_TGCA'GG
Cuts at: 1235 2047 1235
Size: 812 4190
ScaI AGT'ACT
Cuts at: 454 4519 454
Size: 4065 937
SexAI A'CCwGG_T
Cuts at: 927 1430 927
Size: 503 4499
SfcI C'TryA_G
Cuts at: 33 495 990 1231 2043 2728 3723 3914 33
Size: 462 495 241 812 685 995 191 1121
Fragments arranged by size:
1121 995 812 685 495 462 241 191
SfoI GGC'GCC
Cuts at: 2432 2680 2432
Size: 248 4754
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5002
SmaI CCC'GGG
Cuts at: 2022 2022
Size: 5002
SmlI C'TyrA_G
Cuts at: 359 2155 3070 3564 3826 359
Size: 1796 915 494 262 1535
Fragments arranged by size:
1796 1535 915 494 262
SnaBI TAC'GTA
Cuts at: 1992 1992
Size: 5002
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5002
SphI G_CATG'C
Cuts at: 2053 2553 2053
Size: 500 4502
StuI AGG'CCT
Cuts at: 517 1108 1237 1810 517
Size: 591 129 573 3709
Fragments arranged by size:
3709 591 573 129
StyI C'CwwG_G
Cuts at: 518 650 779 1238 1356 2134 2488 518
Size: 132 129 459 118 778 354 3032
Fragments arranged by size:
3032 778 459 354 132 129 118
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1821 1954 2583 2716 3175 3386 1821
Size: 133 629 133 459 211 3437
Fragments arranged by size:
3437 629 459 211 133 133
TatI w'GTAC_w
Cuts at: 452 1739 4517 452
Size: 1287 2778 937
Fragments arranged by size:
2778 1287 937
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 133 165 1718 1750 4519 4551 133
Size: 32 1553 32 2769 32 584
Fragments arranged by size:
2769 1553 584 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2797 2797
Size: 5002
XbaI T'CTAG_A
Cuts at: 2031 2031
Size: 5002
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 301 657 2495 301
Size: 356 1838 2808
Fragments arranged by size:
2808 1838 356
XmaI C'CCGG_G
Cuts at: 2020 2020
Size: 5002
ZraI GAC'GTC
Cuts at: 1971 1971
Size: 5002
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NruI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PstI PvuI PvuII RsrII SacI SalI SanDI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SnaBI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AfeI AflII AhdI AleI AloI ApaI AscI AvrII
BaeI BbvCI BfrBI BglI BmtI BsaXI BsiWI BsrGI
BssHII BstEII BstZ17I ClaI CspCI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NsiI PacI
PmlI PshAI PsiI PspOMI PspXI PsrI SacII SfiI
SgrAI SrfI SspI SwaI XhoI XmnI