Restriction map for
pF1KSDB0055
(Circular) (Six-base) MAPSORT of: pF1KSDB0055.seq Check: 5411 from: 1 to: 7135
pF1KSDB0055 7135 bp
With 182 enzymes: SgfI *
June 20, 2008 10:36 ..
AatII G_ACGT'C
Cuts at: 401 1664 401
Size: 1263 5872
AccI GT'mk_AC
Cuts at: 257 2540 3413 3833 4171 257
Size: 2283 873 420 338 3221
Fragments arranged by size:
3221 2283 873 420 338
Acc65I G'GTAC_C
Cuts at: 3594 4149 3594
Size: 555 6580
AclI AA'CG_TT
Cuts at: 3534 6975 3534
Size: 3441 3694
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1033 1122 2768 3070 4958 5390 6139 6407 1033
Size: 89 1646 302 1888 432 749 268 1761
Fragments arranged by size:
1888 1761 1646 749 432 302 268 89
AflII C'TTAA_G
Cuts at: 2896 2896
Size: 7135
AflIII A'CryG_T
Cuts at: 3125 3196 4485 5591 6568 3125
Size: 71 1289 1106 977 3692
Fragments arranged by size:
3692 1289 1106 977 71
AgeI A'CCGG_T
Cuts at: 5523 5523
Size: 7135
AleI CACnn'nnGTG
Cuts at: 2224 2224
Size: 7135
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 701 2370 3332 701
Size: 1669 962 4504
Fragments arranged by size:
4504 1669 962
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 616 648 1910 1942 616
Size: 32 1262 32 5809
Fragments arranged by size:
5809 1262 32 32
AlwNI CAG_nnn'CTG
Cuts at: 391 522 1145 4438 6007 391
Size: 131 623 3293 1569 1519
Fragments arranged by size:
3293 1569 1519 623 131
ApaLI G'TGCA_C
Cuts at: 1365 5905 1365
Size: 4540 2595
ApoI r'AATT_y
Cuts at: 3941 3966 4137 6488 6744 3941
Size: 25 171 2351 256 4332
Fragments arranged by size:
4332 2351 256 171 25
AseI AT'TA_AT
Cuts at: 20 20
Size: 7135
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7135
AvaI C'yCGr_G
Cuts at: 4153 4153
Size: 7135
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3061 3094 3061
Size: 33 7102
BamHI G'GATC_C
Cuts at: 2553 2862 4158 2553
Size: 309 1296 5530
Fragments arranged by size:
5530 1296 309
BanI G'GyrC_C
Cuts at: 2812 3163 3594 4149 4563 4811 4846 2812
Size: 351 431 555 414 248 35 5101
Fragments arranged by size:
5101 555 431 414 351 248 35
BanII G_rGCy'C
Cuts at: 780 2295 3146 3557 4147 5177 780
Size: 1515 851 411 590 1030 2738
Fragments arranged by size:
2738 1515 1030 851 590 411
BbeI G_GCGC'C
Cuts at: 4567 4815 4567
Size: 248 6887
BbsI GAAGACnn'nnnn_
Cuts at: 1049 1326 1656 1664 2512 2668 4042 1049
Size: 277 330 8 848 156 1374 4142
Fragments arranged by size:
4142 1374 848 330 277 156 8
BbvCI CC'TCA_GC
Cuts at: 2974 2974
Size: 7135
BciVI GTATCCnnnnn_n'
Cuts at: 5031 5794 7135 5031
Size: 763 1341 5031
Fragments arranged by size:
5031 1341 763
BclI T'GATC_A
Cuts at: 2394 2799 4653 2394
Size: 405 1854 4876
Fragments arranged by size:
4876 1854 405
BfrBI ATG'CAT
Cuts at: 678 2357 678
Size: 1679 5456
BglI GCCn_nnn'nGGC
Cuts at: 3141 3574 3141
Size: 433 6702
BglII A'GATC_T
Cuts at: 4648 6687 4648
Size: 2039 5096
BlpI GC'TnA_GC
Cuts at: 4061 4108 4245 4061
Size: 47 137 6951
Fragments arranged by size:
6951 137 47
Bme1580I G_kGCm'C
Cuts at: 1369 2231 3168 3345 4758 4851 5909 1369
Size: 862 937 177 1413 93 1058 2595
Fragments arranged by size:
2595 1413 1058 937 862 177 93
BmgBI CAC'GTC
Cuts at: 2051 2051
Size: 7135
BmrI ACTGGGnnnn_n'
Cuts at: 853 1528 4092 4345 4426 4761 6907 853
Size: 675 2564 253 81 335 2146 1081
Fragments arranged by size:
2564 2146 1081 675 335 253 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5500 5500
Size: 7135
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 175 413 451 577 3669 3784 3802 175
Size: 238 38 126 3092 115 18 3508
Fragments arranged by size:
3508 3092 238 126 115 38 18
Bpu10I CC'TnA_GC
Cuts at: 2155 2974 2155
Size: 819 6316
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 252 1105 3541 4309 5188 5682 5980 252
Size: 853 2436 768 879 494 298 1407
Fragments arranged by size:
2436 1407 879 853 768 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 6839 6839
Size: 7135
BsaAI yAC'GTr
Cuts at: 4125 4486 4125
Size: 361 6774
BsaBI GATnn'nnATC
Cuts at: 6 4647 6686 6
Size: 4641 2039 455
Fragments arranged by size:
4641 2039 455
BsaHI Gr'CG_yC
Cuts at: 398 1661 4564 4812 5516 7012 398
Size: 1263 2903 248 704 1496 521
Fragments arranged by size:
2903 1496 1263 704 521 248
BsaWI w'CCGG_w
Cuts at: 1259 4322 4843 5523 5797 5944 6355 1259
Size: 3063 521 680 274 147 411 2039
Fragments arranged by size:
3063 2039 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 358 388 616 646 3112 3142 358
Size: 30 228 30 2466 30 4351
Fragments arranged by size:
4351 2466 228 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1553 1553
Size: 7135
BseYI C'CCAG_C
Cuts at: 3233 3569 4558 5316 5895 6391 3233
Size: 336 989 758 579 496 3977
Fragments arranged by size:
3977 989 758 579 496 336
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2504 3823 2504
Size: 1319 5816
BsiEI CG_ry'CG
Cuts at: 104 4675 4721 5931 104
Size: 4571 46 1210 1308
Fragments arranged by size:
4571 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 227 1369 3110 3125 3368 3557 4147 4925 5115
Size: 1142 1741 15 243 189 590 778 190
Cuts at: 5115 5909 227
Size: 794 1453
Fragments arranged by size:
1741 1453 1142 794 778 590 243 190
189 15
BsmI GAATG_Cn'
Cuts at: 1014 2359 3010 1014
Size: 1345 651 5139
Fragments arranged by size:
5139 1345 651
BsmBI CGTCTCn'nnnn_
Cuts at: 389 389
Size: 7135
Bsp1286I G_dGCh'C
Cuts at: 227 780 1369 2231 2295 3110 3125 3146 3168
Size: 553 589 862 64 815 15 21 22
Cuts at: 3168 3345 3368 3557 4147 4758 4851 4925 5115
Size: 177 23 189 590 611 93 74 190
Cuts at: 5115 5177 5909 227
Size: 62 732 1453
Fragments arranged by size:
1453 862 815 732 611 590 589 553
190 189 177 93 74 64 62 23
22 21 15
BspEI T'CCGG_A
Cuts at: 6355 6355
Size: 7135
BspHI T'CATG_A
Cuts at: 6311 6311
Size: 7135
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2110 3568 4183 4397 4699 5080 2110
Size: 1458 615 214 302 381 4165
Fragments arranged by size:
4165 1458 615 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3729 5425 5479 6965 7130 50
Size: 3679 1696 54 1486 165 55
Fragments arranged by size:
3679 1696 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3092 5045 3092
Size: 1953 5182
BsrFI r'CCGG_y
Cuts at: 1196 2149 5131 5312 5523 1196
Size: 953 2982 181 211 2808
Fragments arranged by size:
2982 2808 953 211 181
BssSI C'ACGA_G
Cuts at: 2701 3169 5404 5764 2701
Size: 468 2235 360 4072
Fragments arranged by size:
4072 2235 468 360
BstAPI GCAn_nnn'nTGC
Cuts at: 3114 4510 3114
Size: 1396 5739
BstBI TT'CG_AA
Cuts at: 110 110
Size: 7135
BstXI CCAn_nnnn'nTGG
Cuts at: 3330 3944 3330
Size: 614 6521
BstYI r'GATC_y
Cuts at: 1 566 2553 2862 4158 4625 4648 4983 5229
Size: 565 1987 309 1296 467 23 335 246
Cuts at: 5229 6243 6329 6341 6687 1
Size: 1014 86 12 346 449
Fragments arranged by size:
1987 1296 1014 565 467 449 346 335
309 246 86 23 12
BstZ17I GTA'TAC
Cuts at: 3414 3834 3414
Size: 420 6715
Bsu36I CC'TnA_GG
Cuts at: 870 2119 2405 870
Size: 1249 286 5600
Fragments arranged by size:
5600 1249 286
BtgI C'CryG_G
Cuts at: 636 1900 2175 3323 3600 636
Size: 1264 275 1148 277 4171
Fragments arranged by size:
4171 1264 1148 277 275
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2600 5083 5264 5427 2600
Size: 2483 181 163 4308
Fragments arranged by size:
4308 2483 181 163
BtsI GCAGTG_nn'
Cuts at: 2541 3642 3657 4376 2541
Size: 1101 15 719 5300
Fragments arranged by size:
5300 1101 719 15
DraI TTT'AAA
Cuts at: 1545 2136 2340 3845 4132 1545
Size: 591 204 1505 287 4548
Fragments arranged by size:
4548 1505 591 287 204
DraIII CAC_nnn'GTG
Cuts at: 1395 3793 1395
Size: 2398 4737
DrdI GACnn_nn'nnGTC
Cuts at: 4839 5699 4839
Size: 860 6275
EaeI y'GGCC_r
Cuts at: 330 380 1851 1961 2967 3320 4718 4892 5283
Size: 50 1471 110 1006 353 1398 174 391
Cuts at: 5283 5310 6657 330
Size: 27 1347 808
Fragments arranged by size:
1471 1398 1347 1006 808 391 353 174
110 50 27
EagI C'GGCC_G
Cuts at: 4718 4718
Size: 7135
EarI CTCTTCn'nnn_
Cuts at: 1147 1739 1970 2744 3329 3625 5156 5366 1147
Size: 592 231 774 585 296 1531 210 2916
Fragments arranged by size:
2916 1531 774 592 585 296 231 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1343 5341 5653 5799 6945 1343
Size: 3998 312 146 1146 1533
Fragments arranged by size:
3998 1533 1146 312 146
EcoICRI GAG'CTC
Cuts at: 3555 4145 3555
Size: 590 6545
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 175 413 451 577 1033 1122 2768 3070 3669
Size: 238 38 126 456 89 1646 302 599
Cuts at: 3669 3784 3802 4958 5390 6139 6407 175
Size: 115 18 1156 432 749 268 903
Fragments arranged by size:
1646 1156 903 749 599 456 432 302
268 238 126 115 89 38 18
EcoNI CCTnn'n_nnAGG
Cuts at: 836 3115 3781 836
Size: 2279 666 4190
Fragments arranged by size:
4190 2279 666
EcoO109I rG'GnC_Cy
Cuts at: 1179 3768 3988 4272 1179
Size: 2589 220 284 4042
Fragments arranged by size:
4042 2589 284 220
EcoRI G'AATT_C
Cuts at: 3966 4137 3966
Size: 171 6964
EcoRV GAT'ATC
Cuts at: 3087 3692 3087
Size: 605 6530
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1570 2893 3870 5152 1570
Size: 1323 977 1282 3553
Fragments arranged by size:
3553 1323 1282 977
FseI GG_CCGG'CC
Cuts at: 2153 2153
Size: 7135
FspI TGC'GCA
Cuts at: 4914 4914
Size: 7135
HaeII r_GCGC'y
Cuts at: 4567 4815 5839 6818 4567
Size: 248 1024 979 4884
Fragments arranged by size:
4884 1024 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 250 282 352 384 462 494 1152 1184 1571
Size: 32 70 32 78 32 658 32 387
Cuts at: 1571 1603 2023 2055 2148 2180 2361 2379 2393
Size: 32 420 32 93 32 181 18 14
Cuts at: 2393 2411 2601 2633 3607 3639 4975 5005 5007
Size: 18 190 32 974 32 1336 30 2
Cuts at: 5007 5037 250
Size: 30 2348
Fragments arranged by size:
2348 1336 974 658 420 387 190 181
93 78 70 32 32 32 32 32
32 32 32 32 30 30 18 18
14 2
HincII GTy'rAC
Cuts at: 514 821 2084 4172 514
Size: 307 1263 2088 3477
Fragments arranged by size:
3477 2088 1263 307
HindIII A'AGCT_T
Cuts at: 2242 3398 2242
Size: 1156 5979
KasI G'GCGC_C
Cuts at: 4563 4811 4563
Size: 248 6887
KpnI G_GTAC'C
Cuts at: 3598 4153 3598
Size: 555 6580
MluI A'CGCG_T
Cuts at: 6568 6568
Size: 7135
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 454 588 1080 2303 5806 5990 454
Size: 134 492 1223 3503 184 1599
Fragments arranged by size:
3503 1599 1223 492 184 134
MscI TGG'CCA
Cuts at: 382 1853 1963 2969 4894 382
Size: 1471 110 1006 1925 2623
Fragments arranged by size:
2623 1925 1471 1006 110
MslI CAynn'nnrTG
Cuts at: 575 2224 2352 3128 3314 5122 5249 575
Size: 1649 128 776 186 1808 127 2461
Fragments arranged by size:
2461 1808 1649 776 186 128 127
MspA1I CmG'CkG
Cuts at: 429 1626 1780 1842 2922 3139 3258 3762 4244
Size: 1197 154 62 1080 217 119 504 482
Cuts at: 4244 4558 4918 5933 6178 429
Size: 314 360 1015 245 1386
Fragments arranged by size:
1386 1197 1080 1015 504 482 360 314
245 217 154 119 62
NaeI GCC'GGC
Cuts at: 2151 5314 2151
Size: 3163 3972
NarI GG'CG_CC
Cuts at: 4564 4812 4564
Size: 248 6887
NcoI C'CATG_G
Cuts at: 636 1900 2175 3323 636
Size: 1264 275 1148 4448
Fragments arranged by size:
4448 1264 1148 275
NgoMIV G'CCGG_C
Cuts at: 2149 5312 2149
Size: 3163 3972
NruI TCG'CGA
Cuts at: 5496 5496
Size: 7135
NsiI A_TGCA'T
Cuts at: 680 2359 680
Size: 1679 5456
NspI r_CATG'y
Cuts at: 1207 3129 3200 4186 4686 5595 1207
Size: 1922 71 986 500 909 2747
Fragments arranged by size:
2747 1922 986 909 500 71
PasI CC'CwG_GG
Cuts at: 695 695
Size: 7135
PciI A'CATG_T
Cuts at: 3125 3196 5591 3125
Size: 71 2395 4669
Fragments arranged by size:
4669 2395 71
PflMI CCAn_nnn'nTGG
Cuts at: 322 492 3261 322
Size: 170 2769 4196
Fragments arranged by size:
4196 2769 170
PfoI T'CCnGG_A
Cuts at: 554 3685 3779 554
Size: 3131 94 3910
Fragments arranged by size:
3910 3131 94
PmeI GTTT'AAAC
Cuts at: 4132 4132
Size: 7135
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 616 648 6274 6306 616
Size: 32 5626 32 1445
Fragments arranged by size:
5626 1445 32 32
PpuMI rG'GwC_Cy
Cuts at: 1179 3768 3988 1179
Size: 2589 220 4326
Fragments arranged by size:
4326 2589 220
PshAI GACnn'nnGTC
Cuts at: 2006 2006
Size: 7135
PsiI TTA'TAA
Cuts at: 1478 1478
Size: 7135
PstI C_TGCA'G
Cuts at: 412 1725 1779 3917 4180 4865 412
Size: 1313 54 2138 263 685 2682
Fragments arranged by size:
2682 2138 1313 685 263 54
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7135
PvuII CAG'CTG
Cuts at: 429 1842 3258 4558 4918 429
Size: 1413 1416 1300 360 2646
Fragments arranged by size:
2646 1416 1413 1300 360
RsrII CG'GwC_CG
Cuts at: 5328 5328
Size: 7135
SacI G_AGCT'C
Cuts at: 3557 4147 3557
Size: 590 6545
SalI G'TCGA_C
Cuts at: 4170 4170
Size: 7135
SanDI GG'GwC_CC
Cuts at: 1179 1179
Size: 7135
SapI GCTCTTCn'nnn_
Cuts at: 1147 1739 5156 5366 1147
Size: 592 3417 210 2916
Fragments arranged by size:
3417 2916 592 210
SbfI CC_TGCA'GG
Cuts at: 4180 4180
Size: 7135
ScaI AGT'ACT
Cuts at: 2995 6652 2995
Size: 3657 3478
SfcI C'TryA_G
Cuts at: 33 408 1508 1721 1775 2930 3913 4176 4861
Size: 375 1100 213 54 1155 983 263 685
Cuts at: 4861 5856 6047 33
Size: 995 191 1121
Fragments arranged by size:
1155 1121 1100 995 983 685 375 263
213 191 54
SfoI GGC'GCC
Cuts at: 4565 4813 4565
Size: 248 6887
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7135
SmaI CCC'GGG
Cuts at: 4155 4155
Size: 7135
SmlI C'TyrA_G
Cuts at: 267 1120 2896 3556 4288 5203 5697 5959 267
Size: 853 1776 660 732 915 494 262 1443
Fragments arranged by size:
1776 1443 915 853 732 660 494 262
SnaBI TAC'GTA
Cuts at: 4125 4125
Size: 7135
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7135
SphI G_CATG'C
Cuts at: 4186 4686 4186
Size: 500 6635
SspI AAT'ATT
Cuts at: 2764 2764
Size: 7135
StuI AGG'CCT
Cuts at: 1637 2755 3898 1637
Size: 1118 1143 4874
Fragments arranged by size:
4874 1143 1118
StyI C'CwwG_G
Cuts at: 636 1900 1989 2175 2260 3323 4267 4621 636
Size: 1264 89 186 85 1063 944 354 3150
Fragments arranged by size:
3150 1264 1063 944 354 186 89 85
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1519 1963 1969 4716 4849 5308 5519 1519
Size: 444 6 2747 133 459 211 3135
Fragments arranged by size:
3135 2747 459 444 211 133 6
TatI w'GTAC_w
Cuts at: 2685 2697 2993 6650 2685
Size: 12 296 3657 3170
Fragments arranged by size:
3657 3170 296 12
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 358 390 953 985 1304 1336 6652 6684 358
Size: 32 563 32 319 32 5316 32 809
Fragments arranged by size:
5316 809 563 319 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 262 1824 2578 4930 262
Size: 1562 754 2352 2467
Fragments arranged by size:
2467 2352 1562 754
XbaI T'CTAG_A
Cuts at: 1449 4164 1449
Size: 2715 4420
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 719 831 857 3255 4628 719
Size: 112 26 2398 1373 3226
Fragments arranged by size:
3226 2398 1373 112 26
XmaI C'CCGG_G
Cuts at: 4153 4153
Size: 7135
XmnI GAAnn'nnTTC
Cuts at: 308 1046 2279 2876 308
Size: 738 1233 597 4567
Fragments arranged by size:
4567 1233 738 597
ZraI GAC'GTC
Cuts at: 399 1662 399
Size: 1263 5872
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI BstZ17I Bsu36I
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FseI FspI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NruI NsiI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AhdI ApaI AscI AvrII BcgI BmtI
BsiWI BsrGI BssHII BstEII ClaI CspCI FspAI HpaI
MfeI NdeI NheI NotI PacI PmlI PspOMI PspXI
PsrI SacII SexAI SfiI SgrAI SrfI SwaI XhoI