Restriction map for
pF1KSDB0057
(Circular) (Six-base) MAPSORT of: pF1KSDB0057.seq Check: 9382 from: 1 to: 5518
pF1KSDB0057 5518 bp
With 182 enzymes: SgfI *
June 20, 2008 10:36 ..
AccI GT'mk_AC
Cuts at: 650 2554 650
Size: 1904 3614
Acc65I G'GTAC_C
Cuts at: 1385 2532 1385
Size: 1147 4371
AclI AA'CG_TT
Cuts at: 5358 5358
Size: 5518
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1315 1768 3341 3773 4522 4790 1315
Size: 453 1573 432 749 268 2043
Fragments arranged by size:
2043 1573 749 453 432 268
AflIII A'CryG_T
Cuts at: 2868 3974 4951 2868
Size: 1106 977 3435
Fragments arranged by size:
3435 1106 977
AgeI A'CCGG_T
Cuts at: 3906 3906
Size: 5518
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1778 1778
Size: 5518
AlwNI CAG_nnn'CTG
Cuts at: 222 543 1590 1971 2115 2411 2821 4390 222
Size: 321 1047 381 144 296 410 1569 1350
Fragments arranged by size:
1569 1350 1047 410 381 321 296 144
ApaI G_GGCC'C
Cuts at: 1399 1399
Size: 5518
ApaLI G'TGCA_C
Cuts at: 4288 4288
Size: 5518
ApoI r'AATT_y
Cuts at: 1270 2299 2520 4871 5127 1270
Size: 1029 221 2351 256 1661
Fragments arranged by size:
2351 1661 1029 256 221
AseI AT'TA_AT
Cuts at: 20 20
Size: 5518
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5518
AvaI C'yCGr_G
Cuts at: 276 466 637 728 930 1232 2201 2536 276
Size: 190 171 91 202 302 969 335 3258
Fragments arranged by size:
3258 969 335 302 202 190 171 91
BamHI G'GATC_C
Cuts at: 2541 2541
Size: 5518
BanI G'GyrC_C
Cuts at: 173 1385 1840 2118 2414 2532 2946 3194 3229
Size: 1212 455 278 296 118 414 248 35
Cuts at: 3229 173
Size: 2462
Fragments arranged by size:
2462 1212 455 414 296 278 248 118
35
BanII G_rGCy'C
Cuts at: 467 1399 1835 2530 3560 467
Size: 932 436 695 1030 2425
Fragments arranged by size:
2425 1030 932 695 436
BbeI G_GCGC'C
Cuts at: 2950 3198 2950
Size: 248 5270
BbvCI CC'TCA_GC
Cuts at: 184 2426 184
Size: 2242 3276
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1759 1793 1759
Size: 34 5484
BciVI GTATCCnnnnn_n'
Cuts at: 3414 4177 5518 3414
Size: 763 1341 3414
Fragments arranged by size:
3414 1341 763
BclI T'GATC_A
Cuts at: 3036 3036
Size: 5518
BglI GCCn_nnn'nGGC
Cuts at: 151 422 1417 2461 151
Size: 271 995 1044 3208
Fragments arranged by size:
3208 1044 995 271
BglII A'GATC_T
Cuts at: 566 3031 5070 566
Size: 2465 2039 1014
Fragments arranged by size:
2465 2039 1014
BlpI GC'TnA_GC
Cuts at: 2628 2628
Size: 5518
Bme1580I G_kGCm'C
Cuts at: 427 1399 1843 3141 3234 4292 427
Size: 972 444 1298 93 1058 1653
Fragments arranged by size:
1653 1298 1058 972 444 93
BmgBI CAC'GTC
Cuts at: 234 2387 234
Size: 2153 3365
BmrI ACTGGGnnnn_n'
Cuts at: 250 1159 1393 2728 2809 3144 5290 250
Size: 909 234 1335 81 335 2146 478
Fragments arranged by size:
2146 1335 909 478 335 234 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 610 780 1200 3883 610
Size: 170 420 2683 2245
Fragments arranged by size:
2683 2245 420 170
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 367 1087 1327 1492 1549 1615 1636 1993 2264
Size: 720 240 165 57 66 21 357 271
Cuts at: 2264 2281 367
Size: 17 3604
Fragments arranged by size:
3604 720 357 271 240 165 66 57
21 17
Bpu10I CC'TnA_GC
Cuts at: 184 2426 184
Size: 2242 3276
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 142 1873 1990 1996 2692 3571 4065 4363 142
Size: 1731 117 6 696 879 494 298 1297
Fragments arranged by size:
1731 1297 879 696 494 298 117 6
BsaI GGTCTCn'nnnn_
Cuts at: 581 5222 581
Size: 4641 877
BsaAI yAC'GTr
Cuts at: 2508 2869 2508
Size: 361 5157
BsaBI GATnn'nnATC
Cuts at: 6 3030 5069 6
Size: 3024 2039 455
Fragments arranged by size:
3024 2039 455
BsaHI Gr'CG_yC
Cuts at: 308 647 2129 2947 3195 3899 5395 308
Size: 339 1482 818 248 704 1496 431
Fragments arranged by size:
1496 1482 818 704 431 339 248
BsaWI w'CCGG_w
Cuts at: 2705 3226 3906 4180 4327 4738 2705
Size: 521 680 274 147 411 3485
Fragments arranged by size:
3485 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 580 610 652 682 1066 1096 2083 2113 580
Size: 30 42 30 384 30 987 30 3985
Fragments arranged by size:
3985 987 384 42 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 211 657 1294 1441 1546 1549 1591 1612 1678
Size: 446 637 147 105 3 42 21 66
Cuts at: 1678 1834 1990 2062 2104 2107 211
Size: 156 156 72 42 3 3622
Fragments arranged by size:
3622 637 446 156 156 147 105 72
66 42 42 21 3 3
BseYI C'CCAG_C
Cuts at: 1301 1323 1460 2941 3699 4278 4774 1301
Size: 22 137 1481 758 579 496 2045
Fragments arranged by size:
2045 1481 758 579 496 137 22
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 822 822
Size: 5518
BsiEI CG_ry'CG
Cuts at: 104 3058 3104 4314 104
Size: 2954 46 1210 1308
Fragments arranged by size:
2954 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 383 503 1063 1835 2530 3308 3498 4292 383
Size: 120 560 772 695 778 190 794 1609
Fragments arranged by size:
1609 794 778 772 695 560 190 120
BsmI GAATG_Cn'
Cuts at: 495 1128 495
Size: 633 4885
Bsp1286I G_dGCh'C
Cuts at: 383 427 467 503 1063 1399 1835 1843 2530
Size: 44 40 36 560 336 436 8 687
Cuts at: 2530 3141 3234 3308 3498 3560 4292 383
Size: 611 93 74 190 62 732 1609
Fragments arranged by size:
1609 732 687 611 560 436 336 190
93 74 62 44 40 36 8
BspEI T'CCGG_A
Cuts at: 4738 4738
Size: 5518
BspHI T'CATG_A
Cuts at: 2063 4694 2063
Size: 2631 2887
BspMI ACCTGCnnnn'nnnn_
Cuts at: 599 1514 1631 2566 2780 3082 3463 599
Size: 915 117 935 214 302 381 2654
Fragments arranged by size:
2654 935 915 381 302 214 117
BsrBI CCG'CTC
Cuts at: 50 524 770 1524 1785 3808 3862 5348 5513
Size: 474 246 754 261 2023 54 1486 165
Cuts at: 5513 50
Size: 55
Fragments arranged by size:
2023 1486 754 474 261 246 165 55
54
BsrDI GCAATG_nn'
Cuts at: 2222 3428 2222
Size: 1206 4312
BsrFI r'CCGG_y
Cuts at: 2069 2125 3514 3695 3906 2069
Size: 56 1389 181 211 3681
Fragments arranged by size:
3681 1389 211 181 56
BssSI C'ACGA_G
Cuts at: 211 856 2327 3787 4147 211
Size: 645 1471 1460 360 1582
Fragments arranged by size:
1582 1471 1460 645 360
BstAPI GCAn_nnn'nTGC
Cuts at: 1590 2893 1590
Size: 1303 4215
BstBI TT'CG_AA
Cuts at: 110 110
Size: 5518
BstEII G'GTnAC_C
Cuts at: 249 249
Size: 5518
BstXI CCAn_nnnn'nTGG
Cuts at: 2217 2217
Size: 5518
BstYI r'GATC_y
Cuts at: 1 218 566 1928 2407 2541 3008 3031 3366
Size: 217 348 1362 479 134 467 23 335
Cuts at: 3366 3612 4626 4712 4724 5070 1
Size: 246 1014 86 12 346 449
Fragments arranged by size:
1362 1014 479 467 449 348 346 335
246 217 134 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 986 1391 986
Size: 405 5113
BtgI C'CryG_G
Cuts at: 125 227 1673 2210 125
Size: 102 1446 537 3433
Fragments arranged by size:
3433 1446 537 102
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3466 3647 3810 3466
Size: 181 163 5174
Fragments arranged by size:
5174 181 163
BtsI GCAGTG_nn'
Cuts at: 963 1258 2104 2174 2759 963
Size: 295 846 70 585 3722
Fragments arranged by size:
3722 846 585 295 70
DraI TTT'AAA
Cuts at: 2515 2515
Size: 5518
DrdI GACnn_nn'nnGTC
Cuts at: 3222 4082 3222
Size: 860 4658
EaeI y'GGCC_r
Cuts at: 128 414 822 1658 2155 3101 3275 3666 3693
Size: 286 408 836 497 946 174 391 27
Cuts at: 3693 5040 128
Size: 1347 606
Fragments arranged by size:
1347 946 836 606 497 408 391 286
174 27
EagI C'GGCC_G
Cuts at: 3101 3101
Size: 5518
EarI CTCTTCn'nnn_
Cuts at: 572 1306 1339 1418 1744 3539 3749 572
Size: 734 33 79 326 1795 210 2341
Fragments arranged by size:
2341 1795 734 326 210 79 33
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1362 1939 2509 3724 4036 4182 5328 1362
Size: 577 570 1215 312 146 1146 1552
Fragments arranged by size:
1552 1215 1146 577 570 312 146
EcoICRI GAG'CTC
Cuts at: 1833 2528 1833
Size: 695 4823
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 367 1087 1315 1327 1492 1549 1615 1636 1768
Size: 720 228 12 165 57 66 21 132
Cuts at: 1768 1993 2264 2281 3341 3773 4522 4790 367
Size: 225 271 17 1060 432 749 268 1095
Fragments arranged by size:
1095 1060 749 720 432 271 268 228
225 165 132 66 57 21 17 12
EcoNI CCTnn'n_nnAGG
Cuts at: 1858 1975 1858
Size: 117 5401
EcoO109I rG'GnC_Cy
Cuts at: 153 362 452 660 895 1245 1395 1620 1968
Size: 209 90 208 235 350 150 225 348
Cuts at: 1968 2222 2655 153
Size: 254 433 3016
Fragments arranged by size:
3016 433 350 348 254 235 225 209
208 150 90
EcoRI G'AATT_C
Cuts at: 1270 2299 2520 1270
Size: 1029 221 4268
Fragments arranged by size:
4268 1029 221
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3535 3535
Size: 5518
FspI TGC'GCA
Cuts at: 3297 3297
Size: 5518
HaeII r_GCGC'y
Cuts at: 2950 3198 4222 5201 2950
Size: 248 1024 979 3267
Fragments arranged by size:
3267 1024 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 310 342 580 612 652 684 3358 3388 3390
Size: 32 238 32 40 32 2674 30 2
Cuts at: 3390 3420 310
Size: 30 2408
Fragments arranged by size:
2674 2408 238 40 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 2555 2555
Size: 5518
KasI G'GCGC_C
Cuts at: 2946 3194 2946
Size: 248 5270
KpnI G_GTAC'C
Cuts at: 1389 2536 1389
Size: 1147 4371
MluI A'CGCG_T
Cuts at: 4951 4951
Size: 5518
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1721 2246 4189 4373 1721
Size: 525 1943 184 2866
Fragments arranged by size:
2866 1943 525 184
MscI TGG'CCA
Cuts at: 130 416 824 3277 130
Size: 286 408 2453 2371
Fragments arranged by size:
2453 2371 408 286
MslI CAynn'nnrTG
Cuts at: 232 748 752 793 838 2215 3505 3632 232
Size: 516 4 41 45 1377 1290 127 2118
Fragments arranged by size:
2118 1377 1290 516 127 45 41 4
MspA1I CmG'CkG
Cuts at: 907 1305 2284 2430 2627 2941 3301 4316 4561
Size: 398 979 146 197 314 360 1015 245
Cuts at: 4561 907
Size: 1864
Fragments arranged by size:
1864 1015 979 398 360 314 245 197
146
NaeI GCC'GGC
Cuts at: 2127 3697 2127
Size: 1570 3948
NarI GG'CG_CC
Cuts at: 2947 3195 2947
Size: 248 5270
NcoI C'CATG_G
Cuts at: 125 227 1673 2210 125
Size: 102 1446 537 3433
Fragments arranged by size:
3433 1446 537 102
NgoMIV G'CCGG_C
Cuts at: 2125 3695 2125
Size: 1570 3948
NruI TCG'CGA
Cuts at: 3879 3879
Size: 5518
NspI r_CATG'y
Cuts at: 706 2455 2569 3069 3978 706
Size: 1749 114 500 909 2246
Fragments arranged by size:
2246 1749 909 500 114
PasI CC'CwG_GG
Cuts at: 1023 1113 1023
Size: 90 5428
PciI A'CATG_T
Cuts at: 3974 3974
Size: 5518
PflMI CCAn_nnn'nTGG
Cuts at: 2115 2411 2115
Size: 296 5222
PfoI T'CCnGG_A
Cuts at: 1103 1469 1550 1811 1931 2078 1103
Size: 366 81 261 120 147 4543
Fragments arranged by size:
4543 366 261 147 120 81
PmeI GTTT'AAAC
Cuts at: 2515 2515
Size: 5518
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1994 2026 4657 4689 1994
Size: 32 2631 32 2823
Fragments arranged by size:
2823 2631 32 32
PpuMI rG'GwC_Cy
Cuts at: 452 660 1245 1620 1968 2222 452
Size: 208 585 375 348 254 3748
Fragments arranged by size:
3748 585 375 348 254 208
PshAI GACnn'nnGTC
Cuts at: 646 646
Size: 5518
PspOMI G'GGCC_C
Cuts at: 1395 1395
Size: 5518
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 523 555 523
Size: 32 5486
PstI C_TGCA'G
Cuts at: 1511 1859 1913 2169 2563 3248 1511
Size: 348 54 256 394 685 3781
Fragments arranged by size:
3781 685 394 348 256 54
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5518
PvuII CAG'CTG
Cuts at: 1305 2284 2941 3301 1305
Size: 979 657 360 3522
Fragments arranged by size:
3522 979 657 360
RsrII CG'GwC_CG
Cuts at: 3711 3711
Size: 5518
SacI G_AGCT'C
Cuts at: 1835 2530 1835
Size: 695 4823
SalI G'TCGA_C
Cuts at: 2553 2553
Size: 5518
SanDI GG'GwC_CC
Cuts at: 2222 2222
Size: 5518
SapI GCTCTTCn'nnn_
Cuts at: 1339 3539 3749 1339
Size: 2200 210 3108
Fragments arranged by size:
3108 2200 210
SbfI CC_TGCA'GG
Cuts at: 1511 2563 1511
Size: 1052 4466
ScaI AGT'ACT
Cuts at: 5035 5035
Size: 5518
SexAI A'CCwGG_T
Cuts at: 542 542
Size: 5518
SfcI C'TryA_G
Cuts at: 33 1507 1855 1909 2165 2559 3244 4239 4430
Size: 1474 348 54 256 394 685 995 191
Cuts at: 4430 33
Size: 1121
Fragments arranged by size:
1474 1121 995 685 394 348 256 191
54
SfiI GGCCn_nnn'nGGCC
Cuts at: 151 151
Size: 5518
SfoI GGC'GCC
Cuts at: 2948 3196 2948
Size: 248 5270
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5518
SmaI CCC'GGG
Cuts at: 278 730 1234 2538 278
Size: 452 504 1304 3258
Fragments arranged by size:
3258 1304 504 452
SmlI C'TyrA_G
Cuts at: 157 1888 2005 2011 2671 3586 4080 4342 157
Size: 1731 117 6 660 915 494 262 1333
Fragments arranged by size:
1731 1333 915 660 494 262 117 6
SnaBI TAC'GTA
Cuts at: 2508 2508
Size: 5518
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5518
SphI G_CATG'C
Cuts at: 2569 3069 2569
Size: 500 5018
StuI AGG'CCT
Cuts at: 984 1411 2184 984
Size: 427 773 4318
Fragments arranged by size:
4318 773 427
StyI C'CwwG_G
Cuts at: 125 140 227 599 1250 1481 1673 2210 2650
Size: 15 87 372 651 231 192 537 440
Cuts at: 2650 3004 125
Size: 354 2639
Fragments arranged by size:
2639 651 537 440 372 354 231 192
87 15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 447 526 760 1078 1404 3099 3232 3691 3902
Size: 79 234 318 326 1695 133 459 211
Cuts at: 3902 447
Size: 2063
Fragments arranged by size:
2063 1695 459 326 318 234 211 133
79
TatI w'GTAC_w
Cuts at: 1664 5033 1664
Size: 3369 2149
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1053 1085 2083 2115 5035 5067 1053
Size: 32 998 32 2920 32 1504
Fragments arranged by size:
2920 1504 998 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3313 3313
Size: 5518
XbaI T'CTAG_A
Cuts at: 2547 2547
Size: 5518
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 391 424 1134 3011 391
Size: 33 710 1877 2898
Fragments arranged by size:
2898 1877 710 33
XmaI C'CCGG_G
Cuts at: 276 728 1232 2536 276
Size: 452 504 1304 3258
Fragments arranged by size:
3258 1304 504 452
XmnI GAAnn'nnTTC
Cuts at: 581 581
Size: 5518
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NruI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PspOMI PsrI PstI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI ScaI SexAI SfcI
SfiI SfoI SgfI SmaI SmlI SnaBI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI AscI
AvrII BaeI BbsI BfrBI BmtI BsiWI BsmBI BsrGI
BssHII BstZ17I ClaI CspCI DraIII EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NsiI PacI
PmlI PsiI PspXI SacII SgrAI SrfI SspI SwaI
XhoI ZraI