Restriction map for
pF1KSDB0058
(Circular) (Six-base) MAPSORT of: pF1KSDB0058.seq Check: 9285 from: 1 to: 6442
pF1KSDB0058 6442 bp
With 182 enzymes: SgfI *
June 20, 2008 10:36 ..
AccI GT'mk_AC
Cuts at: 1715 2525 3227 3478 1715
Size: 810 702 251 4679
Fragments arranged by size:
4679 810 702 251
Acc65I G'GTAC_C
Cuts at: 2198 3456 2198
Size: 1258 5184
AclI AA'CG_TT
Cuts at: 6282 6282
Size: 6442
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 400 668 937 1249 1587 1940 3382 4265 4697
Size: 268 269 312 338 353 1442 883 432
Cuts at: 4697 5446 5714 400
Size: 749 268 1128
Fragments arranged by size:
1442 1128 883 749 432 353 338 312
269 268 268
AflIII A'CryG_T
Cuts at: 2365 3792 4898 5875 2365
Size: 1427 1106 977 2932
Fragments arranged by size:
2932 1427 1106 977
AgeI A'CCGG_T
Cuts at: 4830 4830
Size: 6442
AleI CACnn'nnGTG
Cuts at: 1171 1171
Size: 6442
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3137 3325 3137
Size: 188 6254
AlwNI CAG_nnn'CTG
Cuts at: 579 1705 2657 3372 3745 5314 579
Size: 1126 952 715 373 1569 1707
Fragments arranged by size:
1707 1569 1126 952 715 373
ApaI G_GGCC'C
Cuts at: 249 1225 249
Size: 976 5466
ApaLI G'TGCA_C
Cuts at: 5212 5212
Size: 6442
ApoI r'AATT_y
Cuts at: 662 3444 5795 6051 662
Size: 2782 2351 256 1053
Fragments arranged by size:
2782 2351 1053 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6442
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6442
AvaI C'yCGr_G
Cuts at: 221 503 724 1109 1643 1685 2202 2901 3257
Size: 282 221 385 534 42 517 699 356
Cuts at: 3257 3460 221
Size: 203 3203
Fragments arranged by size:
3203 699 534 517 385 356 282 221
203 42
BamHI G'GATC_C
Cuts at: 1322 3465 1322
Size: 2143 4299
BanI G'GyrC_C
Cuts at: 973 1051 1518 2198 2571 2935 3456 3870 4118
Size: 78 467 680 373 364 521 414 248
Cuts at: 4118 4153 973
Size: 35 3262
Fragments arranged by size:
3262 680 521 467 414 373 364 248
78 35
BanII G_rGCy'C
Cuts at: 249 452 1225 1697 2135 2209 2681 2847 3294
Size: 203 773 472 438 74 472 166 447
Cuts at: 3294 3454 4484 249
Size: 160 1030 2207
Fragments arranged by size:
2207 1030 773 472 472 447 438 203
166 160 74
BbeI G_GCGC'C
Cuts at: 977 1055 1522 3874 4122 977
Size: 78 467 2352 248 3297
Fragments arranged by size:
3297 2352 467 248 78
BbsI GAAGACnn'nnnn_
Cuts at: 656 1318 1773 2703 656
Size: 662 455 930 4395
Fragments arranged by size:
4395 930 662 455
BbvCI CC'TCA_GC
Cuts at: 192 473 192
Size: 281 6161
BciVI GTATCCnnnnn_n'
Cuts at: 4338 5101 6442 4338
Size: 763 1341 4338
Fragments arranged by size:
4338 1341 763
BclI T'GATC_A
Cuts at: 311 1952 2820 3032 3348 3960 311
Size: 1641 868 212 316 612 2793
Fragments arranged by size:
2793 1641 868 612 316 212
BglI GCCn_nnn'nGGC
Cuts at: 2425 2425
Size: 6442
BglII A'GATC_T
Cuts at: 2532 3955 5994 2532
Size: 1423 2039 2980
Fragments arranged by size:
2980 2039 1423
BlpI GC'TnA_GC
Cuts at: 277 3552 277
Size: 3275 3167
Bme1580I G_kGCm'C
Cuts at: 249 1225 1760 2290 2486 2591 4065 4158 5216
Size: 976 535 530 196 105 1474 93 1058
Cuts at: 5216 249
Size: 1475
Fragments arranged by size:
1475 1474 1058 976 535 530 196 105
93
BmgBI CAC'GTC
Cuts at: 549 2366 549
Size: 1817 4625
BmrI ACTGGGnnnn_n'
Cuts at: 252 1328 1736 1761 3652 3733 4068 6214 252
Size: 1076 408 25 1891 81 335 2146 480
Fragments arranged by size:
2146 1891 1076 480 408 335 81 25
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4807 4807
Size: 6442
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2231 2429 2861 2874 2231
Size: 198 432 13 5799
Fragments arranged by size:
5799 432 198 13
Bpu10I CC'TnA_GC
Cuts at: 192 473 192
Size: 281 6161
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 415 3616 4495 4989 5287 415
Size: 3201 879 494 298 1570
Fragments arranged by size:
3201 1570 879 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 340 1870 2892 3211 6146 340
Size: 1530 1022 319 2935 636
Fragments arranged by size:
2935 1530 1022 636 319
BsaAI yAC'GTr
Cuts at: 3432 3793 3432
Size: 361 6081
BsaBI GATnn'nnATC
Cuts at: 6 1138 1327 1771 3954 5993 6
Size: 1132 189 444 2183 2039 455
Fragments arranged by size:
2183 2039 1132 455 444 189
BsaHI Gr'CG_yC
Cuts at: 563 791 974 1052 1465 1519 2165 3871 4119
Size: 228 183 78 413 54 646 1706 248
Cuts at: 4119 4823 6319 563
Size: 704 1496 686
Fragments arranged by size:
1706 1496 704 686 646 413 248 228
183 78 54
BsaWI w'CCGG_w
Cuts at: 1898 2113 3411 3629 4150 4830 5104 5251 5662
Size: 215 1298 218 521 680 274 147 411
Cuts at: 5662 1898
Size: 2678
Fragments arranged by size:
2678 1298 680 521 411 274 218 215
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 749 779 2191 2221 2465 2495 2540 2570 2872
Size: 30 1412 30 244 30 45 30 302
Cuts at: 2872 2902 749
Size: 30 4289
Fragments arranged by size:
4289 1412 302 244 45 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 490 1429 2373 2740 3119 490
Size: 939 944 367 379 3813
Fragments arranged by size:
3813 944 939 379 367
BseYI C'CCAG_C
Cuts at: 390 1057 1474 2872 3312 3865 4623 5202 5698
Size: 667 417 1398 440 553 758 579 496
Cuts at: 5698 390
Size: 1134
Fragments arranged by size:
1398 1134 758 667 579 553 496 440
417
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 3106 3106
Size: 6442
BsiEI CG_ry'CG
Cuts at: 104 3982 4028 5238 104
Size: 3878 46 1210 1308
Fragments arranged by size:
3878 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 1238 1459 1594 1832 2135 2681 3454 4232 4422
Size: 221 135 238 303 546 773 778 190
Cuts at: 4422 5216 1238
Size: 794 2464
Fragments arranged by size:
2464 794 778 773 546 303 238 221
190 135
BsmBI CGTCTCn'nnnn_
Cuts at: 1682 2841 1682
Size: 1159 5283
Bsp1286I G_dGCh'C
Cuts at: 249 452 1225 1238 1459 1594 1697 1760 1832
Size: 203 773 13 221 135 103 63 72
Cuts at: 1832 2135 2209 2290 2486 2591 2681 2847 3294
Size: 303 74 81 196 105 90 166 447
Cuts at: 3294 3454 4065 4158 4232 4422 4484 5216 249
Size: 160 611 93 74 190 62 732 1475
Fragments arranged by size:
1475 773 732 611 447 303 221 203
196 190 166 160 135 105 103 93
90 81 74 74 72 63 62 13
BspEI T'CCGG_A
Cuts at: 1898 2113 3411 5662 1898
Size: 215 1298 2251 2678
Fragments arranged by size:
2678 2251 1298 215
BspHI T'CATG_A
Cuts at: 1103 2645 5618 1103
Size: 1542 2973 1927
Fragments arranged by size:
2973 1927 1542
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2001 2568 2643 3490 3704 4006 4387 2001
Size: 567 75 847 214 302 381 4056
Fragments arranged by size:
4056 847 567 381 302 214 75
BsrBI CCG'CTC
Cuts at: 50 1853 4732 4786 6272 6437 50
Size: 1803 2879 54 1486 165 55
Fragments arranged by size:
2879 1803 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 881 4352 881
Size: 3471 2971
BsrFI r'CCGG_y
Cuts at: 1388 4438 4619 4830 1388
Size: 3050 181 211 3000
Fragments arranged by size:
3050 3000 211 181
BsrGI T'GTAC_A
Cuts at: 986 986
Size: 6442
BssSI C'ACGA_G
Cuts at: 532 1941 2536 4711 5071 532
Size: 1409 595 2175 360 1903
Fragments arranged by size:
2175 1903 1409 595 360
BstAPI GCAn_nnn'nTGC
Cuts at: 1659 3817 1659
Size: 2158 4284
BstBI TT'CG_AA
Cuts at: 110 110
Size: 6442
BstEII G'GTnAC_C
Cuts at: 3046 3046
Size: 6442
BstXI CCAn_nnnn'nTGG
Cuts at: 1339 2217 1339
Size: 878 5564
BstYI r'GATC_y
Cuts at: 1 947 1133 1322 1573 1766 1895 2532 3089
Size: 946 186 189 251 193 129 637 557
Cuts at: 3089 3465 3932 3955 4290 4536 5550 5636 5648
Size: 376 467 23 335 246 1014 86 12
Cuts at: 5648 5994 1
Size: 346 449
Fragments arranged by size:
1014 946 637 557 467 449 376 346
335 251 246 193 189 186 129 86
23 12
BstZ17I GTA'TAC
Cuts at: 2526 2526
Size: 6442
Bsu36I CC'TnA_GG
Cuts at: 611 1931 611
Size: 1320 5122
BtgI C'CryG_G
Cuts at: 385 956 1709 1871 2156 2591 2993 3385 385
Size: 571 753 162 285 435 402 392 3442
Fragments arranged by size:
3442 753 571 435 402 392 285 162
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1140 1890 2675 4390 4571 4734 1140
Size: 750 785 1715 181 163 2848
Fragments arranged by size:
2848 1715 785 750 181 163
BtsI GCAGTG_nn'
Cuts at: 794 1424 3683 794
Size: 630 2259 3553
Fragments arranged by size:
3553 2259 630
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 303 338 2025 2060 303
Size: 35 1687 35 4685
Fragments arranged by size:
4685 1687 35 35
DraI TTT'AAA
Cuts at: 3439 3439
Size: 6442
DraIII CAC_nnn'GTG
Cuts at: 3054 3054
Size: 6442
DrdI GACnn_nn'nnGTC
Cuts at: 4146 5006 4146
Size: 860 5582
EaeI y'GGCC_r
Cuts at: 131 668 779 962 1361 2180 2400 2540 4025
Size: 537 111 183 399 819 220 140 1485
Cuts at: 4025 4199 4590 4617 5964 131
Size: 174 391 27 1347 609
Fragments arranged by size:
1485 1347 819 609 537 399 391 220
183 174 140 111 27
EagI C'GGCC_G
Cuts at: 4025 4025
Size: 6442
EarI CTCTTCn'nnn_
Cuts at: 694 1254 1606 2714 3349 4463 4673 694
Size: 560 352 1108 635 1114 210 2463
Fragments arranged by size:
2463 1114 1108 635 560 352 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1978 4648 4960 5106 6252 1978
Size: 2670 312 146 1146 2168
Fragments arranged by size:
2670 2168 1146 312 146
EcoICRI GAG'CTC
Cuts at: 2133 2679 3452 2133
Size: 546 773 5123
Fragments arranged by size:
5123 773 546
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 400 668 937 1249 1587 1940 2231 2429 2861
Size: 268 269 312 338 353 291 198 432
Cuts at: 2861 2874 3382 4265 4697 5446 5714 400
Size: 13 508 883 432 749 268 1128
Fragments arranged by size:
1128 883 749 508 432 432 353 338
312 291 269 268 268 198 13
EcoNI CCTnn'n_nnAGG
Cuts at: 2607 2607
Size: 6442
EcoO109I rG'GnC_Cy
Cuts at: 1221 1222 1935 2739 3248 3579 1221
Size: 1 713 804 509 331 4084
Fragments arranged by size:
4084 804 713 509 331 1
EcoRI G'AATT_C
Cuts at: 3444 3444
Size: 6442
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 184 942 4459 184
Size: 758 3517 2167
Fragments arranged by size:
3517 2167 758
FspI TGC'GCA
Cuts at: 4221 4221
Size: 6442
HaeII r_GCGC'y
Cuts at: 977 1055 1522 3874 4122 5146 6125 977
Size: 78 467 2352 248 1024 979 1294
Fragments arranged by size:
2352 1294 1024 979 467 248 78
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 303 335 749 781 1139 1171 1681 1713 1967
Size: 32 414 32 358 32 510 32 254
Cuts at: 1967 1999 2498 2530 2663 2695 2805 2837 2872
Size: 32 499 32 133 32 110 32 35
Cuts at: 2872 2904 3074 3106 4282 4312 4314 4344 303
Size: 32 170 32 1176 30 2 30 2401
Fragments arranged by size:
2401 1176 510 499 414 358 254 170
133 110 35 32 32 32 32 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 348 1020 3479 348
Size: 672 2459 3311
Fragments arranged by size:
3311 2459 672
KasI G'GCGC_C
Cuts at: 973 1051 1518 3870 4118 973
Size: 78 467 2352 248 3297
Fragments arranged by size:
3297 2352 467 248 78
KpnI G_GTAC'C
Cuts at: 2202 3460 2202
Size: 1258 5184
MluI A'CGCG_T
Cuts at: 5875 5875
Size: 6442
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1983 2150 5113 5297 1983
Size: 167 2963 184 3128
Fragments arranged by size:
3128 2963 184 167
MscI TGG'CCA
Cuts at: 670 2182 2402 2542 4201 670
Size: 1512 220 140 1659 2911
Fragments arranged by size:
2911 1659 1512 220 140
MslI CAynn'nnrTG
Cuts at: 1124 1171 2215 2797 3082 3307 3424 4429 4556
Size: 47 1044 582 285 225 117 1005 127
Cuts at: 4556 1124
Size: 3010
Fragments arranged by size:
3010 1044 1005 582 285 225 127 117
47
MspA1I CmG'CkG
Cuts at: 309 1417 1451 2818 3551 3865 4225 5240 5485
Size: 1108 34 1367 733 314 360 1015 245
Cuts at: 5485 309
Size: 1266
Fragments arranged by size:
1367 1266 1108 1015 733 360 314 245
34
NaeI GCC'GGC
Cuts at: 4621 4621
Size: 6442
NarI GG'CG_CC
Cuts at: 974 1052 1519 3871 4119 974
Size: 78 467 2352 248 3297
Fragments arranged by size:
3297 2352 467 248 78
NcoI C'CATG_G
Cuts at: 956 1709 1871 2591 2993 956
Size: 753 162 720 402 4405
Fragments arranged by size:
4405 753 720 402 162
NdeI CA'TA_TG
Cuts at: 3402 3402
Size: 6442
NgoMIV G'CCGG_C
Cuts at: 4619 4619
Size: 6442
NruI TCG'CGA
Cuts at: 4803 4803
Size: 6442
NspI r_CATG'y
Cuts at: 1638 1662 2124 2697 3081 3234 3429 3493 3993
Size: 24 462 573 384 153 195 64 500
Cuts at: 3993 4902 1638
Size: 909 3178
Fragments arranged by size:
3178 909 573 500 462 384 195 153
64 24
PasI CC'CwG_GG
Cuts at: 1380 1380
Size: 6442
PciI A'CATG_T
Cuts at: 4898 4898
Size: 6442
PflMI CCAn_nnn'nTGG
Cuts at: 288 1628 288
Size: 1340 5102
PfoI T'CCnGG_A
Cuts at: 2247 2247
Size: 6442
PmeI GTTT'AAAC
Cuts at: 3439 3439
Size: 6442
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5581 5613 5581
Size: 32 6410
PpuMI rG'GwC_Cy
Cuts at: 2739 3248 2739
Size: 509 5933
PspOMI G'GGCC_C
Cuts at: 245 1221 245
Size: 976 5466
PstI C_TGCA'G
Cuts at: 260 2442 3487 4172 260
Size: 2182 1045 685 2530
Fragments arranged by size:
2530 2182 1045 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6442
PvuII CAG'CTG
Cuts at: 309 2818 3865 4225 309
Size: 2509 1047 360 2526
Fragments arranged by size:
2526 2509 1047 360
RsrII CG'GwC_CG
Cuts at: 4635 4635
Size: 6442
SacI G_AGCT'C
Cuts at: 2135 2681 3454 2135
Size: 546 773 5123
Fragments arranged by size:
5123 773 546
SalI G'TCGA_C
Cuts at: 3477 3477
Size: 6442
SapI GCTCTTCn'nnn_
Cuts at: 4463 4673 4463
Size: 210 6232
SbfI CC_TGCA'GG
Cuts at: 3487 3487
Size: 6442
ScaI AGT'ACT
Cuts at: 1045 2779 5959 1045
Size: 1734 3180 1528
Fragments arranged by size:
3180 1734 1528
SexAI A'CCwGG_T
Cuts at: 2882 2882
Size: 6442
SfcI C'TryA_G
Cuts at: 33 256 2438 3483 4168 5163 5354 33
Size: 223 2182 1045 685 995 191 1121
Fragments arranged by size:
2182 1121 1045 995 685 223 191
SfoI GGC'GCC
Cuts at: 975 1053 1520 3872 4120 975
Size: 78 467 2352 248 3297
Fragments arranged by size:
3297 2352 467 248 78
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6442
SmaI CCC'GGG
Cuts at: 1645 2204 3462 1645
Size: 559 1258 4625
Fragments arranged by size:
4625 1258 559
SmlI C'TyrA_G
Cuts at: 430 724 3595 4510 5004 5266 430
Size: 294 2871 915 494 262 1606
Fragments arranged by size:
2871 1606 915 494 294 262
SnaBI TAC'GTA
Cuts at: 3432 3432
Size: 6442
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6442
SphI G_CATG'C
Cuts at: 1638 1662 2697 3493 3993 1638
Size: 24 1035 796 500 4087
Fragments arranged by size:
4087 1035 796 500 24
StyI C'CwwG_G
Cuts at: 134 566 782 956 1616 1709 1871 2591 2939
Size: 432 216 174 660 93 162 720 348
Cuts at: 2939 2993 3574 3928 134
Size: 54 581 354 2648
Fragments arranged by size:
2648 720 660 581 432 354 348 216
174 162 93 54
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 541 1136 1425 2009 4023 4156 4615 4826 541
Size: 595 289 584 2014 133 459 211 2157
Fragments arranged by size:
2157 2014 595 584 459 289 211 133
TatI w'GTAC_w
Cuts at: 710 986 1043 2777 5957 710
Size: 276 57 1734 3180 1195
Fragments arranged by size:
3180 1734 1195 276 57
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2465 2497 5959 5991 2465
Size: 32 3462 32 2916
Fragments arranged by size:
3462 2916 32 32
Tth111I GACn'n_nGTC
Cuts at: 517 1675 2884 4237 517
Size: 1158 1209 1353 2722
Fragments arranged by size:
2722 1353 1209 1158
XbaI T'CTAG_A
Cuts at: 3471 3471
Size: 6442
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1371 3935 1371
Size: 2564 3878
XhoI C'TCGA_G
Cuts at: 724 724
Size: 6442
XmaI C'CCGG_G
Cuts at: 1643 2202 3460 1643
Size: 559 1258 4625
Fragments arranged by size:
4625 1258 559
XmnI GAAnn'nnTTC
Cuts at: 208 208
Size: 6442
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NruI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PspOMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SnaBI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AloI AscI AvrII
BaeI BcgI BfrBI BmtI BsiWI BsmI BssHII ClaI
EcoRV FseI FspAI HindIII HpaI MfeI NheI NotI
NsiI PacI PmlI PshAI PsiI PspXI PsrI SacII
SanDI SfiI SgrAI SrfI SspI StuI SwaI ZraI