Restriction map for
pF1KSDB0059
(Circular) (Six-base) MAPSORT of: pF1KSDB0059.seq Check: 9859 from: 1 to: 6550
pF1KSDB0059 6550 bp
With 182 enzymes: SgfI *
June 20, 2008 10:36 ..
AccI GT'mk_AC
Cuts at: 3197 3586 3197
Size: 389 6161
Acc65I G'GTAC_C
Cuts at: 3564 3564
Size: 6550
AclI AA'CG_TT
Cuts at: 6390 6390
Size: 6550
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1515 1834 2044 4373 4805 5554 5822 1515
Size: 319 210 2329 432 749 268 2243
Fragments arranged by size:
2329 2243 749 432 319 268 210
AflIII A'CryG_T
Cuts at: 3900 5006 5983 3900
Size: 1106 977 4467
Fragments arranged by size:
4467 1106 977
AgeI A'CCGG_T
Cuts at: 4938 4938
Size: 6550
AleI CACnn'nnGTG
Cuts at: 998 1522 998
Size: 524 6026
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 491 523 655 687 491
Size: 32 132 32 6354
Fragments arranged by size:
6354 132 32 32
AlwNI CAG_nnn'CTG
Cuts at: 357 585 633 1410 1956 2265 2499 2512 3853
Size: 228 48 777 546 309 234 13 1341
Cuts at: 3853 5422 357
Size: 1569 1485
Fragments arranged by size:
1569 1485 1341 777 546 309 234 228
48 13
ApaI G_GGCC'C
Cuts at: 774 2310 3460 3508 774
Size: 1536 1150 48 3816
Fragments arranged by size:
3816 1536 1150 48
ApaLI G'TGCA_C
Cuts at: 545 5320 545
Size: 4775 1775
ApoI r'AATT_y
Cuts at: 2110 3552 5903 6159 2110
Size: 1442 2351 256 2501
Fragments arranged by size:
2501 2351 1442 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6550
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6550
AvaI C'yCGr_G
Cuts at: 930 1045 1797 2093 2978 3337 3377 3496 3568
Size: 115 752 296 885 359 40 119 72
Cuts at: 3568 930
Size: 3912
Fragments arranged by size:
3912 885 752 359 296 119 115 72
40
BamHI G'GATC_C
Cuts at: 3573 3573
Size: 6550
BanI G'GyrC_C
Cuts at: 244 1041 1144 1330 1801 2395 3415 3529 3564
Size: 797 103 186 471 594 1020 114 35
Cuts at: 3564 3978 4226 4261 244
Size: 414 248 35 2533
Fragments arranged by size:
2533 1020 797 594 471 414 248 186
114 103 35 35
BanII G_rGCy'C
Cuts at: 138 774 981 1507 2310 2523 2698 2915 3460
Size: 636 207 526 803 213 175 217 545
Cuts at: 3460 3508 3562 4592 138
Size: 48 54 1030 2096
Fragments arranged by size:
2096 1030 803 636 545 526 217 213
207 175 54 48
BbeI G_GCGC'C
Cuts at: 3982 4230 3982
Size: 248 6302
BbsI GAAGACnn'nnnn_
Cuts at: 1350 2048 2520 3389 3415 1350
Size: 698 472 869 26 4485
Fragments arranged by size:
4485 869 698 472 26
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 261 295 364 398 261
Size: 34 69 34 6413
Fragments arranged by size:
6413 69 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4446 5209 6550 4446
Size: 763 1341 4446
Fragments arranged by size:
4446 1341 763
BclI T'GATC_A
Cuts at: 4068 4068
Size: 6550
BglI GCCn_nnn'nGGC
Cuts at: 281 692 722 281
Size: 411 30 6109
Fragments arranged by size:
6109 411 30
BglII A'GATC_T
Cuts at: 2048 4063 6102 2048
Size: 2015 2039 2496
Fragments arranged by size:
2496 2039 2015
BlpI GC'TnA_GC
Cuts at: 622 961 3660 622
Size: 339 2699 3512
Fragments arranged by size:
3512 2699 339
Bme1580I G_kGCm'C
Cuts at: 549 727 774 1046 1149 1335 1804 2310 2400
Size: 178 47 272 103 186 469 506 90
Cuts at: 2400 2986 3236 3353 3418 3460 3508 4173 4266
Size: 586 250 117 65 42 48 665 93
Cuts at: 4266 5324 549
Size: 1058 1775
Fragments arranged by size:
1775 1058 665 586 506 469 272 250
186 178 117 103 93 90 65 48
47 42
BmgBI CAC'GTC
Cuts at: 237 237
Size: 6550
BmrI ACTGGGnnnn_n'
Cuts at: 1227 3181 3348 3444 3760 3841 4176 6322 1227
Size: 1954 167 96 316 81 335 2146 1455
Fragments arranged by size:
2146 1954 1455 335 316 167 96 81
BmtI G_CTAG'C
Cuts at: 3283 3283
Size: 6550
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2370 3105 4915 2370
Size: 735 1810 4005
Fragments arranged by size:
4005 1810 735
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 410 439 607 995 1432 1615 1843 1900 1946
Size: 29 168 388 437 183 228 57 46
Cuts at: 1946 2206 2479 2794 2893 3100 410
Size: 260 273 315 99 207 3860
Fragments arranged by size:
3860 437 388 315 273 260 228 207
183 168 99 57 46 29
Bpu10I CC'TnA_GC
Cuts at: 1762 2422 2882 1762
Size: 660 460 5430
Fragments arranged by size:
5430 660 460
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3724 4603 5097 5395 3724
Size: 879 494 298 4879
Fragments arranged by size:
4879 879 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 1572 2440 6254 1572
Size: 868 3814 1868
Fragments arranged by size:
3814 1868 868
BsaAI yAC'GTr
Cuts at: 3540 3901 3540
Size: 361 6189
BsaBI GATnn'nnATC
Cuts at: 6 3515 4062 6101 6
Size: 3509 547 2039 455
Fragments arranged by size:
3509 2039 547 455
BsaHI Gr'CG_yC
Cuts at: 684 1844 3979 4227 4931 6427 684
Size: 1160 2135 248 704 1496 807
Fragments arranged by size:
2135 1496 1160 807 704 248
BsaWI w'CCGG_w
Cuts at: 1724 3737 4258 4938 5212 5359 5770 1724
Size: 2013 521 680 274 147 411 2504
Fragments arranged by size:
2504 2013 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 475 491 505 521 1532 1562 475
Size: 16 14 16 1011 30 5463
Fragments arranged by size:
5463 1011 30 16 16 14
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 436 751 3097 3418 436
Size: 315 2346 321 3568
Fragments arranged by size:
3568 2346 321 315
BseYI C'CCAG_C
Cuts at: 1176 1222 1669 1993 2000 2217 2222 2258 2373
Size: 46 447 324 7 217 5 36 115
Cuts at: 2373 2570 2914 3265 3501 3973 4731 5310 5806
Size: 197 344 351 236 472 758 579 496
Cuts at: 5806 1176
Size: 1920
Fragments arranged by size:
1920 758 579 496 472 447 351 344
324 236 217 197 115 46 36 7
5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 283 971 2272 2498 283
Size: 688 1301 226 4335
Fragments arranged by size:
4335 1301 688 226
BsiEI CG_ry'CG
Cuts at: 104 4090 4136 5346 104
Size: 3986 46 1210 1308
Fragments arranged by size:
3986 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 348 549 623 1529 3562 4340 4530 5324 348
Size: 201 74 906 2033 778 190 794 1574
Fragments arranged by size:
2033 1574 906 794 778 201 190 74
BsmI GAATG_Cn'
Cuts at: 2355 2355
Size: 6550
BsmBI CGTCTCn'nnnn_
Cuts at: 583 1835 3064 583
Size: 1252 1229 4069
Fragments arranged by size:
4069 1252 1229
Bsp1286I G_dGCh'C
Cuts at: 138 348 549 623 727 774 981 1046 1149
Size: 210 201 74 104 47 207 65 103
Cuts at: 1149 1335 1507 1529 1804 2310 2400 2523 2698
Size: 186 172 22 275 506 90 123 175
Cuts at: 2698 2915 2986 3236 3353 3418 3460 3508 3562
Size: 217 71 250 117 65 42 48 54
Cuts at: 3562 4173 4266 4340 4530 4592 5324 138
Size: 611 93 74 190 62 732 1364
Fragments arranged by size:
1364 732 611 506 275 250 217 210
207 201 190 186 175 172 123 117
104 103 93 90 74 74 71 65
65 62 54 48 47 42 22
BspEI T'CCGG_A
Cuts at: 5770 5770
Size: 6550
BspHI T'CATG_A
Cuts at: 2724 5726 2724
Size: 3002 3548
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1502 2213 2754 3598 3812 4114 4495 1502
Size: 711 541 844 214 302 381 3557
Fragments arranged by size:
3557 844 711 541 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2625 2904 4840 4894 6380 6545 50
Size: 2575 279 1936 54 1486 165 55
Fragments arranged by size:
2575 1936 1486 279 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1658 2549 4460 1658
Size: 891 1911 3748
Fragments arranged by size:
3748 1911 891
BsrFI r'CCGG_y
Cuts at: 302 869 4546 4727 4938 302
Size: 567 3677 181 211 1914
Fragments arranged by size:
3677 1914 567 211 181
BssSI C'ACGA_G
Cuts at: 2516 4819 5179 2516
Size: 2303 360 3887
Fragments arranged by size:
3887 2303 360
BstAPI GCAn_nnn'nTGC
Cuts at: 1330 2499 3925 1330
Size: 1169 1426 3955
Fragments arranged by size:
3955 1426 1169
BstBI TT'CG_AA
Cuts at: 110 110
Size: 6550
BstEII G'GTnAC_C
Cuts at: 2196 2403 2196
Size: 207 6343
BstXI CCAn_nnnn'nTGG
Cuts at: 523 1038 2133 2236 2457 2997 3216 523
Size: 515 1095 103 221 540 219 3857
Fragments arranged by size:
3857 1095 540 515 221 219 103
BstYI r'GATC_y
Cuts at: 1 581 1226 2012 2048 3573 4040 4063 4398
Size: 580 645 786 36 1525 467 23 335
Cuts at: 4398 4644 5658 5744 5756 6102 1
Size: 246 1014 86 12 346 449
Fragments arranged by size:
1525 1014 786 645 580 467 449 346
335 246 86 36 23 12
BstZ17I GTA'TAC
Cuts at: 3198 3198
Size: 6550
Bsu36I CC'TnA_GG
Cuts at: 1639 3424 1639
Size: 1785 4765
BtgI C'CryG_G
Cuts at: 125 1368 2126 2450 3209 125
Size: 1243 758 324 759 3466
Fragments arranged by size:
3466 1243 759 758 324
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4498 4679 4842 4498
Size: 181 163 6206
Fragments arranged by size:
6206 181 163
BtsI GCAGTG_nn'
Cuts at: 1331 1529 2284 3283 3791 1331
Size: 198 755 999 508 4090
Fragments arranged by size:
4090 999 755 508 198
DraI TTT'AAA
Cuts at: 3547 3547
Size: 6550
DraIII CAC_nnn'GTG
Cuts at: 1808 2164 1808
Size: 356 6194
DrdI GACnn_nn'nnGTC
Cuts at: 1642 4254 5114 1642
Size: 2612 860 3078
Fragments arranged by size:
3078 2612 860
EaeI y'GGCC_r
Cuts at: 128 635 1126 1617 1947 2477 2561 2929 2987
Size: 507 491 491 330 530 84 368 58
Cuts at: 2987 4133 4307 4698 4725 6072 128
Size: 1146 174 391 27 1347 606
Fragments arranged by size:
1347 1146 606 530 507 491 491 391
368 330 174 84 58 27
EagI C'GGCC_G
Cuts at: 4133 4133
Size: 6550
EarI CTCTTCn'nnn_
Cuts at: 211 283 368 403 950 1166 1534 2383 2584
Size: 72 85 35 547 216 368 849 201
Cuts at: 2584 4571 4781 211
Size: 1987 210 1980
Fragments arranged by size:
1987 1980 849 547 368 216 210 201
85 72 35
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4756 5068 5214 6360 4756
Size: 312 146 1146 4946
Fragments arranged by size:
4946 1146 312 146
EcoICRI GAG'CTC
Cuts at: 3560 3560
Size: 6550
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 410 439 607 995 1432 1515 1615 1834 1843
Size: 29 168 388 437 83 100 219 9
Cuts at: 1843 1900 1946 2044 2206 2479 2794 2893 3100
Size: 57 46 98 162 273 315 99 207
Cuts at: 3100 4373 4805 5554 5822 410
Size: 1273 432 749 268 1138
Fragments arranged by size:
1273 1138 749 437 432 388 315 273
268 219 207 168 162 100 99 98
83 57 46 29 9
EcoNI CCTnn'n_nnAGG
Cuts at: 1018 1018
Size: 6550
EcoO109I rG'GnC_Cy
Cuts at: 158 354 520 630 770 771 1293 2307 2788
Size: 196 166 110 140 1 522 1014 481
Cuts at: 2788 3456 3505 3687 158
Size: 668 49 182 3021
Fragments arranged by size:
3021 1014 668 522 481 196 182 166
140 110 49 1
EcoRI G'AATT_C
Cuts at: 2110 3552 2110
Size: 1442 5108
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1897 4567 1897
Size: 2670 3880
FspI TGC'GCA
Cuts at: 253 1018 4329 253
Size: 765 3311 2474
Fragments arranged by size:
3311 2474 765
HaeII r_GCGC'y
Cuts at: 1031 1071 1167 3982 4230 5254 6233 1031
Size: 40 96 2815 248 1024 979 1348
Fragments arranged by size:
2815 1348 1024 979 248 96 40
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 431 463 1218 1250 1532 1564 2510 2542 4390
Size: 32 755 32 282 32 946 32 1848
Cuts at: 4390 4420 4422 4452 431
Size: 30 2 30 2529
Fragments arranged by size:
2529 1848 946 755 282 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 3587 3587
Size: 6550
HindIII A'AGCT_T
Cuts at: 1879 1879
Size: 6550
KasI G'GCGC_C
Cuts at: 3978 4226 3978
Size: 248 6302
KpnI G_GTAC'C
Cuts at: 3568 3568
Size: 6550
MluI A'CGCG_T
Cuts at: 5983 5983
Size: 6550
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 632 2139 2690 5221 5405 632
Size: 1507 551 2531 184 1777
Fragments arranged by size:
2531 1777 1507 551 184
MscI TGG'CCA
Cuts at: 637 1128 1949 2479 2563 2931 4309 637
Size: 491 821 530 84 368 1378 2878
Fragments arranged by size:
2878 1378 821 530 491 368 84
MslI CAynn'nnrTG
Cuts at: 554 998 1522 1823 1894 2131 2837 3133 3214
Size: 444 524 301 71 237 706 296 81
Cuts at: 3214 4537 4664 554
Size: 1323 127 2440
Fragments arranged by size:
2440 1323 706 524 444 301 296 237
127 81 71
MspA1I CmG'CkG
Cuts at: 730 840 2004 2070 3008 3094 3659 3973 4333
Size: 110 1164 66 938 86 565 314 360
Cuts at: 4333 5348 5593 730
Size: 1015 245 1687
Fragments arranged by size:
1687 1164 1015 938 565 360 314 245
110 86 66
NaeI GCC'GGC
Cuts at: 4729 4729
Size: 6550
NarI GG'CG_CC
Cuts at: 3979 4227 3979
Size: 248 6302
NcoI C'CATG_G
Cuts at: 125 1368 2126 3209 125
Size: 1243 758 1083 3466
Fragments arranged by size:
3466 1243 1083 758
NgoMIV G'CCGG_C
Cuts at: 4727 4727
Size: 6550
NheI G'CTAG_C
Cuts at: 3279 3279
Size: 6550
NruI TCG'CGA
Cuts at: 4911 4911
Size: 6550
NspI r_CATG'y
Cuts at: 2922 3049 3601 4101 5010 2922
Size: 127 552 500 909 4462
Fragments arranged by size:
4462 909 552 500 127
PasI CC'CwG_GG
Cuts at: 2691 2691
Size: 6550
PciI A'CATG_T
Cuts at: 5006 5006
Size: 6550
PflMI CCAn_nnn'nTGG
Cuts at: 357 495 585 633 2179 2265 357
Size: 138 90 48 1546 86 4642
Fragments arranged by size:
4642 1546 138 90 86 48
PfoI T'CCnGG_A
Cuts at: 163 584 641 1859 2431 163
Size: 421 57 1218 572 4282
Fragments arranged by size:
4282 1218 572 421 57
PmeI GTTT'AAAC
Cuts at: 3547 3547
Size: 6550
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 491 523 5689 5721 491
Size: 32 5166 32 1320
Fragments arranged by size:
5166 1320 32 32
PpuMI rG'GwC_Cy
Cuts at: 630 1293 630
Size: 663 5887
PspOMI G'GGCC_C
Cuts at: 770 2306 3456 3504 770
Size: 1536 1150 48 3816
Fragments arranged by size:
3816 1536 1150 48
PstI C_TGCA'G
Cuts at: 2069 3595 4280 2069
Size: 1526 685 4339
Fragments arranged by size:
4339 1526 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6550
PvuII CAG'CTG
Cuts at: 730 840 2070 3008 3094 3973 4333 730
Size: 110 1230 938 86 879 360 2947
Fragments arranged by size:
2947 1230 938 879 360 110 86
RsrII CG'GwC_CG
Cuts at: 4743 4743
Size: 6550
SacI G_AGCT'C
Cuts at: 3562 3562
Size: 6550
SalI G'TCGA_C
Cuts at: 3585 3585
Size: 6550
SapI GCTCTTCn'nnn_
Cuts at: 211 368 950 1166 1534 4571 4781 211
Size: 157 582 216 368 3037 210 1980
Fragments arranged by size:
3037 1980 582 368 216 210 157
SbfI CC_TGCA'GG
Cuts at: 3595 3595
Size: 6550
ScaI AGT'ACT
Cuts at: 3088 6067 3088
Size: 2979 3571
SfcI C'TryA_G
Cuts at: 33 435 1677 1908 2065 3018 3591 4276 5271
Size: 402 1242 231 157 953 573 685 995
Cuts at: 5271 5462 33
Size: 191 1121
Fragments arranged by size:
1242 1121 995 953 685 573 402 231
191 157
SfoI GGC'GCC
Cuts at: 3980 4228 3980
Size: 248 6302
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6550
SmaI CCC'GGG
Cuts at: 2095 2980 3570 2095
Size: 885 590 5075
Fragments arranged by size:
5075 885 590
SmlI C'TyrA_G
Cuts at: 3703 4618 5112 5374 3703
Size: 915 494 262 4879
Fragments arranged by size:
4879 915 494 262
SnaBI TAC'GTA
Cuts at: 3540 3540
Size: 6550
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6550
SphI G_CATG'C
Cuts at: 3601 4101 3601
Size: 500 6050
StuI AGG'CCT
Cuts at: 395 1795 395
Size: 1400 5150
StyI C'CwwG_G
Cuts at: 125 151 690 1368 2126 2670 3209 3682 4036
Size: 26 539 678 758 544 539 473 354
Cuts at: 4036 125
Size: 2639
Fragments arranged by size:
2639 758 678 544 539 539 473 354
26
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 232 331 1132 1318 1614 1844 2014 3401 4131
Size: 99 801 186 296 230 170 1387 730
Cuts at: 4131 4264 4723 4934 232
Size: 133 459 211 1848
Fragments arranged by size:
1848 1387 801 730 459 296 230 211
186 170 133 99
TatI w'GTAC_w
Cuts at: 1196 3086 3258 6065 1196
Size: 1890 172 2807 1681
Fragments arranged by size:
2807 1890 1681 172
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 475 507 660 692 3458 3490 6067 6099 475
Size: 32 153 32 2766 32 2577 32 926
Fragments arranged by size:
2766 2577 926 153 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1831 1902 4345 1831
Size: 71 2443 4036
Fragments arranged by size:
4036 2443 71
XbaI T'CTAG_A
Cuts at: 3579 3579
Size: 6550
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 703 2556 3216 4043 703
Size: 1853 660 827 3210
Fragments arranged by size:
3210 1853 827 660
XmaI C'CCGG_G
Cuts at: 2093 2978 3568 2093
Size: 885 590 5075
Fragments arranged by size:
5075 885 590
XmnI GAAnn'nnTTC
Cuts at: 1357 1357
Size: 6550
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AloI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NheI NruI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PspOMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SnaBI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AscI AvrII
BaeI BbvCI BfrBI BsiWI BsrGI BssHII ClaI CspCI
EcoRV FseI FspAI HpaI MfeI NdeI NotI NsiI
PacI PmlI PshAI PsiI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SrfI SspI SwaI XhoI ZraI