Restriction map for
pF1KSDB0061
(Circular) (Six-base) MAPSORT of: pF1KSDB0061.seq Check: 769 from: 1 to: 6433
pF1KSDB0061 6433 bp
With 182 enzymes: SgfI *
June 20, 2008 10:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2099 2099
Size: 6433
AatII G_ACGT'C
Cuts at: 1664 1664
Size: 6433
AccI GT'mk_AC
Cuts at: 3469 3469
Size: 6433
Acc65I G'GTAC_C
Cuts at: 3447 3447
Size: 6433
AclI AA'CG_TT
Cuts at: 6273 6273
Size: 6433
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1402 1975 2721 4256 4688 5437 5705 1402
Size: 573 746 1535 432 749 268 2130
Fragments arranged by size:
2130 1535 749 746 573 432 268
AfeI AGC'GCT
Cuts at: 142 244 2698 142
Size: 102 2454 3877
Fragments arranged by size:
3877 2454 102
AflIII A'CryG_T
Cuts at: 401 1514 3783 4889 5866 401
Size: 1113 2269 1106 977 968
Fragments arranged by size:
2269 1113 1106 977 968
AgeI A'CCGG_T
Cuts at: 4821 4821
Size: 6433
AhdI GACnn_n'nnGTC
Cuts at: 3251 3251
Size: 6433
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1291 2042 3013 1291
Size: 751 971 4711
Fragments arranged by size:
4711 971 751
AlwNI CAG_nnn'CTG
Cuts at: 765 798 939 989 1112 1560 1572 2091 2391
Size: 33 141 50 123 448 12 519 300
Cuts at: 2391 2580 3214 3736 5305 765
Size: 189 634 522 1569 1893
Fragments arranged by size:
1893 1569 634 522 519 448 300 189
141 123 50 33 12
ApaI G_GGCC'C
Cuts at: 3022 3076 3270 3022
Size: 54 194 6185
Fragments arranged by size:
6185 194 54
ApaLI G'TGCA_C
Cuts at: 1854 5203 1854
Size: 3349 3084
ApoI r'AATT_y
Cuts at: 2011 3142 3435 5786 6042 2011
Size: 1131 293 2351 256 2402
Fragments arranged by size:
2402 2351 1131 293 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6433
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6433
AvaI C'yCGr_G
Cuts at: 577 1010 1759 1904 2041 2374 2954 3068 3110
Size: 433 749 145 137 333 580 114 42
Cuts at: 3110 3351 3451 577
Size: 241 100 3559
Fragments arranged by size:
3559 749 580 433 333 241 145 137
114 100 42
BamHI G'GATC_C
Cuts at: 917 3456 917
Size: 2539 3894
BanI G'GyrC_C
Cuts at: 1787 2240 2788 2997 3052 3260 3447 3861 4109
Size: 453 548 209 55 208 187 414 248
Cuts at: 4109 4144 1787
Size: 35 4076
Fragments arranged by size:
4076 548 453 414 248 209 208 187
55 35
BanII G_rGCy'C
Cuts at: 1196 1486 2117 2893 3022 3076 3270 3445 4475
Size: 290 631 776 129 54 194 175 1030
Cuts at: 4475 1196
Size: 3154
Fragments arranged by size:
3154 1030 776 631 290 194 175 129
54
BbeI G_GCGC'C
Cuts at: 1791 2244 3865 4113 1791
Size: 453 1621 248 4111
Fragments arranged by size:
4111 1621 453 248
BbsI GAAGACnn'nnnn_
Cuts at: 221 478 1001 2655 221
Size: 257 523 1654 3999
Fragments arranged by size:
3999 1654 523 257
BbvCI CC'TCA_GC
Cuts at: 2596 2596
Size: 6433
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1320 1354 2077 2111 2194 2228 2682 2716 1320
Size: 34 723 34 83 34 454 34 5037
Fragments arranged by size:
5037 723 454 83 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4329 5092 6433 4329
Size: 763 1341 4329
Fragments arranged by size:
4329 1341 763
BclI T'GATC_A
Cuts at: 3951 3951
Size: 6433
BglI GCCn_nnn'nGGC
Cuts at: 629 1115 1490 2058 3386 629
Size: 486 375 568 1328 3676
Fragments arranged by size:
3676 1328 568 486 375
BglII A'GATC_T
Cuts at: 683 1298 3416 3946 5985 683
Size: 615 2118 530 2039 1131
Fragments arranged by size:
2118 2039 1131 615 530
BlpI GC'TnA_GC
Cuts at: 1480 3304 3543 1480
Size: 1824 239 4370
Fragments arranged by size:
4370 1824 239
Bme1580I G_kGCm'C
Cuts at: 1858 3002 3022 3055 3076 3117 3270 4056 4149
Size: 1144 20 33 21 41 153 786 93
Cuts at: 4149 5207 1858
Size: 1058 3084
Fragments arranged by size:
3084 1144 1058 786 153 93 41 33
21 20
BmgBI CAC'GTC
Cuts at: 543 1515 2048 3118 543
Size: 972 533 1070 3858
Fragments arranged by size:
3858 1070 972 533
BmrI ACTGGGnnnn_n'
Cuts at: 354 1920 3643 3724 4059 6205 354
Size: 1566 1723 81 335 2146 582
Fragments arranged by size:
2146 1723 1566 582 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1711 2611 4798 1711
Size: 900 2187 3346
Fragments arranged by size:
3346 2187 900
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 721 1632 2200 2383 2674 3052 3065 721
Size: 911 568 183 291 378 13 4089
Fragments arranged by size:
4089 911 568 378 291 183 13
Bpu10I CC'TnA_GC
Cuts at: 2596 2596
Size: 6433
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2821 3607 4486 4980 5278 2821
Size: 786 879 494 298 3976
Fragments arranged by size:
3976 879 786 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 1656 1885 6137 1656
Size: 229 4252 1952
Fragments arranged by size:
4252 1952 229
BsaAI yAC'GTr
Cuts at: 2247 3282 3408 3423 3784 2247
Size: 1035 126 15 361 4896
Fragments arranged by size:
4896 1035 361 126 15
BsaBI GATnn'nnATC
Cuts at: 6 3945 5984 6
Size: 3939 2039 455
Fragments arranged by size:
3939 2039 455
BsaHI Gr'CG_yC
Cuts at: 218 1661 1788 2081 2241 2479 3862 4110 4814
Size: 1443 127 293 160 238 1383 248 704
Cuts at: 4814 6310 218
Size: 1496 341
Fragments arranged by size:
1496 1443 1383 704 341 293 248 238
160 127
BsaWI w'CCGG_w
Cuts at: 236 407 813 1745 2255 3620 4141 4821 5095
Size: 171 406 932 510 1365 521 680 274
Cuts at: 5095 5242 5653 236
Size: 147 411 1016
Fragments arranged by size:
1365 1016 932 680 521 510 411 406
274 171 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 3025 3055 3025
Size: 30 6403
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1411 1930 2197 2281 2380 2719 1411
Size: 519 267 84 99 339 5125
Fragments arranged by size:
5125 519 339 267 99 84
BseYI C'CCAG_C
Cuts at: 466 925 1053 1112 1792 2774 3236 3856 4614
Size: 459 128 59 680 982 462 620 758
Cuts at: 4614 5193 5689 466
Size: 579 496 1210
Fragments arranged by size:
1210 982 758 680 620 579 496 462
459 128 59
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1426 1426
Size: 6433
BsiEI CG_ry'CG
Cuts at: 104 330 2442 2850 3973 4019 5229 104
Size: 226 2112 408 1123 46 1210 1308
Fragments arranged by size:
2112 1308 1210 1123 408 226 46
BsiHKAI G_wGCw'C
Cuts at: 287 1858 2140 2837 2893 3134 3445 4223 4413
Size: 1571 282 697 56 241 311 778 190
Cuts at: 4413 5207 287
Size: 794 1513
Fragments arranged by size:
1571 1513 794 778 697 311 282 241
190 56
BsmBI CGTCTCn'nnnn_
Cuts at: 1682 2038 1682
Size: 356 6077
Bsp1286I G_dGCh'C
Cuts at: 287 1196 1486 1858 2117 2140 2837 2893 3002
Size: 909 290 372 259 23 697 56 109
Cuts at: 3002 3022 3055 3076 3117 3134 3270 3445 4056
Size: 20 33 21 41 17 136 175 611
Cuts at: 4056 4149 4223 4413 4475 5207 287
Size: 93 74 190 62 732 1513
Fragments arranged by size:
1513 909 732 697 611 372 290 259
190 175 136 109 93 74 62 56
41 33 23 21 20 17
BspEI T'CCGG_A
Cuts at: 236 1745 2255 5653 236
Size: 1509 510 3398 1016
Fragments arranged by size:
3398 1509 1016 510
BspHI T'CATG_A
Cuts at: 806 1496 5609 806
Size: 690 4113 1630
Fragments arranged by size:
4113 1630 690
BspMI ACCTGCnnnn'nnnn_
Cuts at: 897 1058 2099 3481 3695 3997 4378 897
Size: 161 1041 1382 214 302 381 2952
Fragments arranged by size:
2952 1382 1041 381 302 214 161
BsrBI CCG'CTC
Cuts at: 50 176 2313 2370 2406 4723 4777 6263 6428
Size: 126 2137 57 36 2317 54 1486 165
Cuts at: 6428 50
Size: 55
Fragments arranged by size:
2317 2137 1486 165 126 57 55 54
36
BsrDI GCAATG_nn'
Cuts at: 221 2997 4343 221
Size: 2776 1346 2311
Fragments arranged by size:
2776 2311 1346
BsrFI r'CCGG_y
Cuts at: 1618 1801 1981 3172 3368 4429 4610 4821 1618
Size: 183 180 1191 196 1061 181 211 3230
Fragments arranged by size:
3230 1191 1061 211 196 183 181 180
BssSI C'ACGA_G
Cuts at: 952 1219 1594 4702 5062 952
Size: 267 375 3108 360 2323
Fragments arranged by size:
3108 2323 375 360 267
BstAPI GCAn_nnn'nTGC
Cuts at: 1572 3808 1572
Size: 2236 4197
BstBI TT'CG_AA
Cuts at: 110 110
Size: 6433
BstEII G'GTnAC_C
Cuts at: 2427 2427
Size: 6433
BstXI CCAn_nnnn'nTGG
Cuts at: 1312 1642 1833 2982 3090 1312
Size: 330 191 1149 108 4655
Fragments arranged by size:
4655 1149 330 191 108
BstYI r'GATC_y
Cuts at: 1 644 683 917 962 1298 3332 3345 3416
Size: 643 39 234 45 336 2034 13 71
Cuts at: 3416 3456 3923 3946 4281 4527 5541 5627 5639
Size: 40 467 23 335 246 1014 86 12
Cuts at: 5639 5985 1
Size: 346 449
Fragments arranged by size:
2034 1014 643 467 449 346 336 335
246 234 86 71 45 40 39 23
13 12
BtgI C'CryG_G
Cuts at: 125 1535 2053 2811 125
Size: 1410 518 758 3747
Fragments arranged by size:
3747 1410 758 518
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2888 4381 4562 4725 2888
Size: 1493 181 163 4596
Fragments arranged by size:
4596 1493 181 163
BtsI GCAGTG_nn'
Cuts at: 1558 2837 3674 1558
Size: 1279 837 4317
Fragments arranged by size:
4317 1279 837
DraI TTT'AAA
Cuts at: 3430 3430
Size: 6433
DraIII CAC_nnn'GTG
Cuts at: 165 1938 165
Size: 1773 4660
DrdI GACnn_nn'nnGTC
Cuts at: 4137 4997 4137
Size: 860 5573
EaeI y'GGCC_r
Cuts at: 1184 1838 2050 2915 3170 3205 3378 4016 4190
Size: 654 212 865 255 35 173 638 174
Cuts at: 4190 4581 4608 5955 1184
Size: 391 27 1347 1662
Fragments arranged by size:
1662 1347 865 654 638 391 255 212
174 173 35 27
EagI C'GGCC_G
Cuts at: 4016 4016
Size: 6433
EarI CTCTTCn'nnn_
Cuts at: 1213 1253 1702 1759 4454 4664 1213
Size: 40 449 57 2695 210 2982
Fragments arranged by size:
2982 2695 449 210 57 40
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 536 3110 4639 4951 5097 6243 536
Size: 2574 1529 312 146 1146 726
Fragments arranged by size:
2574 1529 1146 726 312 146
EcoICRI GAG'CTC
Cuts at: 2891 3443 2891
Size: 552 5881
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 721 1402 1632 1975 2200 2383 2674 2721 3052
Size: 681 230 343 225 183 291 47 331
Cuts at: 3052 3065 4256 4688 5437 5705 721
Size: 13 1191 432 749 268 1449
Fragments arranged by size:
1449 1191 749 681 432 343 331 291
268 230 225 183 47 13
EcoNI CCTnn'n_nnAGG
Cuts at: 346 1186 1222 2179 2182 2629 346
Size: 840 36 957 3 447 4150
Fragments arranged by size:
4150 957 840 447 36 3
EcoO109I rG'GnC_Cy
Cuts at: 773 1589 1901 2524 2720 3018 3019 3072 3073
Size: 816 312 623 196 298 1 53 1
Cuts at: 3073 3267 3387 3570 773
Size: 194 120 183 3636
Fragments arranged by size:
3636 816 623 312 298 196 194 183
120 53 1 1
EcoRI G'AATT_C
Cuts at: 3142 3435 3142
Size: 293 6140
EcoRV GAT'ATC
Cuts at: 552 552
Size: 6433
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 159 2484 4450 159
Size: 2325 1966 2142
Fragments arranged by size:
2325 2142 1966
FspI TGC'GCA
Cuts at: 607 1975 4212 607
Size: 1368 2237 2828
Fragments arranged by size:
2828 2237 1368
HaeII r_GCGC'y
Cuts at: 144 246 1619 1791 2244 2700 3865 4113 5137
Size: 102 1373 172 453 456 1165 248 1024
Cuts at: 5137 6116 144
Size: 979 461
Fragments arranged by size:
1373 1165 1024 979 461 456 453 248
172 102
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 3025 3057 3237 3269 4273 4303 4305 4335 3025
Size: 32 180 32 1004 30 2 30 5123
Fragments arranged by size:
5123 1004 180 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2106 3470 2106
Size: 1364 5069
HindIII A'AGCT_T
Cuts at: 2282 2282
Size: 6433
KasI G'GCGC_C
Cuts at: 1787 2240 3861 4109 1787
Size: 453 1621 248 4111
Fragments arranged by size:
4111 1621 453 248
KpnI G_GTAC'C
Cuts at: 3451 3451
Size: 6433
MluI A'CGCG_T
Cuts at: 5866 5866
Size: 6433
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 590 713 1727 2312 2830 5104 5288 590
Size: 123 1014 585 518 2274 184 1735
Fragments arranged by size:
2274 1735 1014 585 518 184 123
MscI TGG'CCA
Cuts at: 1186 1840 2917 4192 1186
Size: 654 1077 1275 3427
Fragments arranged by size:
3427 1275 1077 654
MslI CAynn'nnrTG
Cuts at: 1138 1273 1846 1969 4420 4547 1138
Size: 135 573 123 2451 127 3024
Fragments arranged by size:
3024 2451 573 135 127 123
MspA1I CmG'CkG
Cuts at: 573 762 939 1067 1112 1421 1610 1667 1874
Size: 189 177 128 45 309 189 57 207
Cuts at: 1874 2055 2813 3542 3856 4216 5231 5476 573
Size: 181 758 729 314 360 1015 245 1530
Fragments arranged by size:
1530 1015 758 729 360 314 309 245
207 189 189 181 177 128 57 45
NaeI GCC'GGC
Cuts at: 1620 1803 1983 3174 3370 4612 1620
Size: 183 180 1191 196 1242 3441
Fragments arranged by size:
3441 1242 1191 196 183 180
NarI GG'CG_CC
Cuts at: 1788 2241 3862 4110 1788
Size: 453 1621 248 4111
Fragments arranged by size:
4111 1621 453 248
NcoI C'CATG_G
Cuts at: 125 125
Size: 6433
NgoMIV G'CCGG_C
Cuts at: 1618 1801 1981 3172 3368 4610 1618
Size: 183 180 1191 196 1242 3441
Fragments arranged by size:
3441 1242 1191 196 183 180
NruI TCG'CGA
Cuts at: 4794 4794
Size: 6433
NspI r_CATG'y
Cuts at: 405 417 3180 3484 3984 4893 405
Size: 12 2763 304 500 909 1945
Fragments arranged by size:
2763 1945 909 500 304 12
PasI CC'CwG_GG
Cuts at: 3090 3090
Size: 6433
PciI A'CATG_T
Cuts at: 401 4889 401
Size: 4488 1945
PflMI CCAn_nnn'nTGG
Cuts at: 765 939 3168 3214 3282 3294 765
Size: 174 2229 46 68 12 3904
Fragments arranged by size:
3904 2229 174 68 46 12
PfoI T'CCnGG_A
Cuts at: 2177 2651 2177
Size: 474 5959
PmeI GTTT'AAAC
Cuts at: 3430 3430
Size: 6433
PmlI CAC'GTG
Cuts at: 2247 2247
Size: 6433
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 634 666 2185 2217 5572 5604 634
Size: 32 1519 32 3355 32 1463
Fragments arranged by size:
3355 1519 1463 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 1589 2524 1589
Size: 935 5498
PspOMI G'GGCC_C
Cuts at: 3018 3072 3266 3018
Size: 54 194 6185
Fragments arranged by size:
6185 194 54
PspXI vC'TCGA_Gb
Cuts at: 577 577
Size: 6433
PstI C_TGCA'G
Cuts at: 563 950 1079 1358 1568 1577 2021 3478 4163
Size: 387 129 279 210 9 444 1457 685
Cuts at: 4163 563
Size: 2833
Fragments arranged by size:
2833 1457 685 444 387 279 210 129
9
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6433
PvuII CAG'CTG
Cuts at: 762 939 1610 3856 4216 762
Size: 177 671 2246 360 2979
Fragments arranged by size:
2979 2246 671 360 177
RsrII CG'GwC_CG
Cuts at: 4626 4626
Size: 6433
SacI G_AGCT'C
Cuts at: 2893 3445 2893
Size: 552 5881
SacII CC_GC'GG
Cuts at: 2056 2814 2056
Size: 758 5675
SalI G'TCGA_C
Cuts at: 3468 3468
Size: 6433
SanDI GG'GwC_CC
Cuts at: 1589 1589
Size: 6433
SapI GCTCTTCn'nnn_
Cuts at: 1759 4454 4664 1759
Size: 2695 210 3528
Fragments arranged by size:
3528 2695 210
SbfI CC_TGCA'GG
Cuts at: 3478 3478
Size: 6433
ScaI AGT'ACT
Cuts at: 637 1438 5950 637
Size: 801 4512 1120
Fragments arranged by size:
4512 1120 801
SexAI A'CCwGG_T
Cuts at: 851 1832 1934 851
Size: 981 102 5350
Fragments arranged by size:
5350 981 102
SfcI C'TryA_G
Cuts at: 33 559 946 1075 1354 1564 1573 2017 2749
Size: 526 387 129 279 210 9 444 732
Cuts at: 2749 3474 4159 5154 5345 33
Size: 725 685 995 191 1121
Fragments arranged by size:
1121 995 732 725 685 526 444 387
279 210 191 129 9
SfiI GGCCn_nnn'nGGCC
Cuts at: 3386 3386
Size: 6433
SfoI GGC'GCC
Cuts at: 1789 2242 3863 4111 1789
Size: 453 1621 248 4111
Fragments arranged by size:
4111 1621 453 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6433
SmaI CCC'GGG
Cuts at: 2956 3070 3112 3453 2956
Size: 114 42 341 5936
Fragments arranged by size:
5936 341 114 42
SmlI C'TyrA_G
Cuts at: 577 2836 3586 4501 4995 5257 577
Size: 2259 750 915 494 262 1753
Fragments arranged by size:
2259 1753 915 750 494 262
SnaBI TAC'GTA
Cuts at: 3423 3423
Size: 6433
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6433
SphI G_CATG'C
Cuts at: 3180 3484 3984 3180
Size: 304 500 5629
Fragments arranged by size:
5629 500 304
StuI AGG'CCT
Cuts at: 583 1738 2233 3063 583
Size: 1155 495 830 3953
Fragments arranged by size:
3953 1155 830 495
StyI C'CwwG_G
Cuts at: 125 509 1187 2141 2918 3565 3919 125
Size: 384 678 954 777 647 354 2639
Fragments arranged by size:
2639 954 777 678 647 384 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2415 2686 3070 4014 4147 4606 4817 2415
Size: 271 384 944 133 459 211 4031
Fragments arranged by size:
4031 944 459 384 271 211 133
TatI w'GTAC_w
Cuts at: 635 1262 1436 5948 635
Size: 627 174 4512 1120
Fragments arranged by size:
4512 1120 627 174
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1501 1533 3392 3424 5950 5982 1501
Size: 32 1859 32 2526 32 1952
Fragments arranged by size:
2526 1952 1859 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 4228 4228
Size: 6433
XbaI T'CTAG_A
Cuts at: 3462 3462
Size: 6433
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 492 696 1098 1639 1833 3926 492
Size: 204 402 541 194 2093 2999
Fragments arranged by size:
2999 2093 541 402 204 194
XhoI C'TCGA_G
Cuts at: 577 577
Size: 6433
XmaI C'CCGG_G
Cuts at: 2954 3068 3110 3451 2954
Size: 114 42 341 5936
Fragments arranged by size:
5936 341 114 42
XmnI GAAnn'nnTTC
Cuts at: 244 244
Size: 6433
ZraI GAC'GTC
Cuts at: 1662 1662
Size: 6433
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AhdI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NruI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PspOMI PspXI PstI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfcI
SfiI SfoI SgfI SmaI SmlI SnaBI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AflII AleI AloI AscI AvrII BaeI BfrBI BmtI
BsiWI BsmI BsrGI BssHII BstZ17I Bsu36I ClaI CspCI
FseI FspAI HpaI MfeI NdeI NheI NotI NsiI
PacI PshAI PsiI PsrI SgrAI SrfI SspI SwaI