Restriction map for
pF1KSDB0062
(Circular) (Six-base) MAPSORT of: pF1KSDB0062.seq Check: 1425 from: 1 to: 5149
pF1KSDB0062 5149 bp
With 182 enzymes: SgfI *
June 20, 2008 10:36 ..
AccI GT'mk_AC
Cuts at: 2185 2185
Size: 5149
Acc65I G'GTAC_C
Cuts at: 2163 2163
Size: 5149
AclI AA'CG_TT
Cuts at: 4989 4989
Size: 5149
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1266 1879 2972 3404 4153 4421 1266
Size: 613 1093 432 749 268 1994
Fragments arranged by size:
1994 1093 749 613 432 268
AflIII A'CryG_T
Cuts at: 2499 3605 4582 2499
Size: 1106 977 3066
Fragments arranged by size:
3066 1106 977
AgeI A'CCGG_T
Cuts at: 3537 3537
Size: 5149
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 416 416
Size: 5149
AlwNI CAG_nnn'CTG
Cuts at: 1183 2452 4021 1183
Size: 1269 1569 2311
Fragments arranged by size:
2311 1569 1269
ApaLI G'TGCA_C
Cuts at: 3919 3919
Size: 5149
ApoI r'AATT_y
Cuts at: 330 917 982 1298 1507 2151 4502 4758 330
Size: 587 65 316 209 644 2351 256 721
Fragments arranged by size:
2351 721 644 587 316 256 209 65
AseI AT'TA_AT
Cuts at: 20 493 578 20
Size: 473 85 4591
Fragments arranged by size:
4591 473 85
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5149
AvaI C'yCGr_G
Cuts at: 1616 2167 1616
Size: 551 4598
BamHI G'GATC_C
Cuts at: 2172 2172
Size: 5149
BanI G'GyrC_C
Cuts at: 963 2163 2577 2825 2860 963
Size: 1200 414 248 35 3252
Fragments arranged by size:
3252 1200 414 248 35
BanII G_rGCy'C
Cuts at: 2161 3191 2161
Size: 1030 4119
BbeI G_GCGC'C
Cuts at: 2581 2829 2581
Size: 248 4901
BbsI GAAGACnn'nnnn_
Cuts at: 2120 2120
Size: 5149
BciVI GTATCCnnnnn_n'
Cuts at: 1371 3045 3808 5149 1371
Size: 1674 763 1341 1371
Fragments arranged by size:
1674 1371 1341 763
BclI T'GATC_A
Cuts at: 2667 2667
Size: 5149
BfrBI ATG'CAT
Cuts at: 1602 1602
Size: 5149
BglII A'GATC_T
Cuts at: 2662 4701 2662
Size: 2039 3110
BlpI GC'TnA_GC
Cuts at: 2259 2259
Size: 5149
Bme1580I G_kGCm'C
Cuts at: 2772 2865 3923 2772
Size: 93 1058 3998
Fragments arranged by size:
3998 1058 93
BmrI ACTGGGnnnn_n'
Cuts at: 153 2359 2440 2775 4921 153
Size: 2206 81 335 2146 381
Fragments arranged by size:
2206 2146 381 335 81
BmtI G_CTAG'C
Cuts at: 2060 2060
Size: 5149
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3514 3514
Size: 5149
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1096 1448 1096
Size: 352 4797
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 210 434 1976 2323 3202 3696 3994 210
Size: 224 1542 347 879 494 298 1365
Fragments arranged by size:
1542 1365 879 494 347 298 224
BsaI GGTCTCn'nnnn_
Cuts at: 2021 4853 2021
Size: 2832 2317
BsaAI yAC'GTr
Cuts at: 2139 2500 2139
Size: 361 4788
BsaBI GATnn'nnATC
Cuts at: 6 890 2661 4700 6
Size: 884 1771 2039 455
Fragments arranged by size:
2039 1771 884 455
BsaHI Gr'CG_yC
Cuts at: 185 2578 2826 3530 5026 185
Size: 2393 248 704 1496 308
Fragments arranged by size:
2393 1496 704 308 248
BsaWI w'CCGG_w
Cuts at: 2336 2857 3537 3811 3958 4369 2336
Size: 521 680 274 147 411 3116
Fragments arranged by size:
3116 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 489 519 489
Size: 30 5119
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1414 1655 1414
Size: 241 4908
BseYI C'CCAG_C
Cuts at: 1943 2066 2572 3330 3909 4405 1943
Size: 123 506 758 579 496 2687
Fragments arranged by size:
2687 758 579 506 496 123
BsiEI CG_ry'CG
Cuts at: 104 2689 2735 3945 104
Size: 2585 46 1210 1308
Fragments arranged by size:
2585 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 2161 2939 3129 3923 2161
Size: 778 190 794 3387
Fragments arranged by size:
3387 794 778 190
BsmI GAATG_Cn'
Cuts at: 1602 1602
Size: 5149
BsmBI CGTCTCn'nnnn_
Cuts at: 192 192
Size: 5149
Bsp1286I G_dGCh'C
Cuts at: 2161 2772 2865 2939 3129 3191 3923 2161
Size: 611 93 74 190 62 732 3387
Fragments arranged by size:
3387 732 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 4369 4369
Size: 5149
BspHI T'CATG_A
Cuts at: 4325 4325
Size: 5149
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2197 2411 2713 3094 2197
Size: 214 302 381 4252
Fragments arranged by size:
4252 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3439 3493 4979 5144 50
Size: 3389 54 1486 165 55
Fragments arranged by size:
3389 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1629 3059 1629
Size: 1430 3719
BsrFI r'CCGG_y
Cuts at: 3145 3326 3537 3145
Size: 181 211 4757
Fragments arranged by size:
4757 211 181
BssSI C'ACGA_G
Cuts at: 3418 3778 3418
Size: 360 4789
BstAPI GCAn_nnn'nTGC
Cuts at: 2524 2524
Size: 5149
BstBI TT'CG_AA
Cuts at: 110 110
Size: 5149
BstXI CCAn_nnnn'nTGG
Cuts at: 1252 1843 1951 1252
Size: 591 108 4450
Fragments arranged by size:
4450 591 108
BstYI r'GATC_y
Cuts at: 1 481 2172 2639 2662 2997 3243 4257 4343
Size: 480 1691 467 23 335 246 1014 86
Cuts at: 4343 4355 4701 1
Size: 12 346 449
Fragments arranged by size:
1691 1014 480 467 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1397 1397
Size: 5149
BtgI C'CryG_G
Cuts at: 361 361
Size: 5149
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3097 3278 3441 3097
Size: 181 163 4805
Fragments arranged by size:
4805 181 163
BtsI GCAGTG_nn'
Cuts at: 2390 2390
Size: 5149
DraI TTT'AAA
Cuts at: 1172 2146 1172
Size: 974 4175
DrdI GACnn_nn'nnGTC
Cuts at: 2853 3713 2853
Size: 860 4289
EaeI y'GGCC_r
Cuts at: 1147 2732 2906 3297 3324 4671 1147
Size: 1585 174 391 27 1347 1625
Fragments arranged by size:
1625 1585 1347 391 174 27
EagI C'GGCC_G
Cuts at: 2732 2732
Size: 5149
EarI CTCTTCn'nnn_
Cuts at: 920 1285 2024 3170 3380 920
Size: 365 739 1146 210 2689
Fragments arranged by size:
2689 1146 739 365 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3355 3667 3813 4959 3355
Size: 312 146 1146 3545
Fragments arranged by size:
3545 1146 312 146
EcoICRI GAG'CTC
Cuts at: 2159 2159
Size: 5149
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1096 1266 1448 1879 2972 3404 4153 4421 1096
Size: 170 182 431 1093 432 749 268 1824
Fragments arranged by size:
1824 1093 749 432 431 268 182 170
EcoO109I rG'GnC_Cy
Cuts at: 502 2286 502
Size: 1784 3365
EcoRI G'AATT_C
Cuts at: 2151 2151
Size: 5149
EcoRV GAT'ATC
Cuts at: 1089 1527 1089
Size: 438 4711
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1337 1884 1963 3166 1337
Size: 547 79 1203 3320
Fragments arranged by size:
3320 1203 547 79
FspI TGC'GCA
Cuts at: 2928 2928
Size: 5149
HaeII r_GCGC'y
Cuts at: 2581 2829 3853 4832 2581
Size: 248 1024 979 2898
Fragments arranged by size:
2898 1024 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 825 857 2989 3019 3021 3051 825
Size: 32 2132 30 2 30 2923
Fragments arranged by size:
2923 2132 32 30 30 2
HincII GTy'rAC
Cuts at: 150 2186 150
Size: 2036 3113
HindIII A'AGCT_T
Cuts at: 639 639
Size: 5149
KasI G'GCGC_C
Cuts at: 2577 2825 2577
Size: 248 4901
KpnI G_GTAC'C
Cuts at: 2167 2167
Size: 5149
MluI A'CGCG_T
Cuts at: 4582 4582
Size: 5149
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1631 3820 4004 1631
Size: 2189 184 2776
Fragments arranged by size:
2776 2189 184
MscI TGG'CCA
Cuts at: 2908 2908
Size: 5149
MslI CAynn'nnrTG
Cuts at: 1804 3136 3263 1804
Size: 1332 127 3690
Fragments arranged by size:
3690 1332 127
MspA1I CmG'CkG
Cuts at: 1931 2258 2572 2932 3947 4192 1931
Size: 327 314 360 1015 245 2888
Fragments arranged by size:
2888 1015 360 327 314 245
NaeI GCC'GGC
Cuts at: 3328 3328
Size: 5149
NarI GG'CG_CC
Cuts at: 2578 2826 2578
Size: 248 4901
NcoI C'CATG_G
Cuts at: 361 361
Size: 5149
NdeI CA'TA_TG
Cuts at: 647 647
Size: 5149
NgoMIV G'CCGG_C
Cuts at: 3326 3326
Size: 5149
NheI G'CTAG_C
Cuts at: 2056 2056
Size: 5149
NruI TCG'CGA
Cuts at: 3510 3510
Size: 5149
NsiI A_TGCA'T
Cuts at: 1604 1604
Size: 5149
NspI r_CATG'y
Cuts at: 1425 2200 2700 3609 1425
Size: 775 500 909 2965
Fragments arranged by size:
2965 909 775 500
PciI A'CATG_T
Cuts at: 3605 3605
Size: 5149
PfoI T'CCnGG_A
Cuts at: 507 507
Size: 5149
PmeI GTTT'AAAC
Cuts at: 2146 2146
Size: 5149
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4288 4320 4288
Size: 32 5117
PpuMI rG'GwC_Cy
Cuts at: 502 502
Size: 5149
PsiI TTA'TAA
Cuts at: 862 862
Size: 5149
PstI C_TGCA'G
Cuts at: 1968 2194 2879 1968
Size: 226 685 4238
Fragments arranged by size:
4238 685 226
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5149
PvuII CAG'CTG
Cuts at: 2572 2932 2572
Size: 360 4789
RsrII CG'GwC_CG
Cuts at: 3342 3342
Size: 5149
SacI G_AGCT'C
Cuts at: 2161 2161
Size: 5149
SalI G'TCGA_C
Cuts at: 2184 2184
Size: 5149
SapI GCTCTTCn'nnn_
Cuts at: 3170 3380 3170
Size: 210 4939
SbfI CC_TGCA'GG
Cuts at: 2194 2194
Size: 5149
ScaI AGT'ACT
Cuts at: 4666 4666
Size: 5149
SexAI A'CCwGG_T
Cuts at: 436 2011 436
Size: 1575 3574
SfcI C'TryA_G
Cuts at: 33 1004 1580 1964 2190 2875 3870 4061 33
Size: 971 576 384 226 685 995 191 1121
Fragments arranged by size:
1121 995 971 685 576 384 226 191
SfoI GGC'GCC
Cuts at: 2579 2827 2579
Size: 248 4901
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5149
SmaI CCC'GGG
Cuts at: 2169 2169
Size: 5149
SmlI C'TyrA_G
Cuts at: 225 449 1991 2302 3217 3711 3973 225
Size: 224 1542 311 915 494 262 1401
Fragments arranged by size:
1542 1401 915 494 311 262 224
SnaBI TAC'GTA
Cuts at: 2139 2139
Size: 5149
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5149
SphI G_CATG'C
Cuts at: 2200 2700 2200
Size: 500 4649
SspI AAT'ATT
Cuts at: 945 945
Size: 5149
StyI C'CwwG_G
Cuts at: 361 1150 1635 2082 2281 2635 361
Size: 789 485 447 199 354 2875
Fragments arranged by size:
2875 789 485 447 354 199
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 354 1969 2730 2863 3322 3533 354
Size: 1615 761 133 459 211 1970
Fragments arranged by size:
1970 1615 761 459 211 133
TatI w'GTAC_w
Cuts at: 564 4664 564
Size: 4100 1049
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4666 4698 4666
Size: 32 5117
Tth111I GACn'n_nGTC
Cuts at: 2944 2944
Size: 5149
XbaI T'CTAG_A
Cuts at: 171 1056 2178 171
Size: 885 1122 3142
Fragments arranged by size:
3142 1122 885
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1252 2642 1252
Size: 1390 3759
XmaI C'CCGG_G
Cuts at: 2167 2167
Size: 5149
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BfrBI BglII BlpI Bme1580I
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NruI
NsiI NspI PciI PfoI PmeI PpiI PpuMI PsiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SnaBI
SpeI SphI SspI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BglI BmgBI Bpu10I
BsgI BsiWI BsrGI BssHII BstEII BstZ17I ClaI CspCI
DraIII EcoNI FseI FspAI HpaI MfeI NotI PacI
PasI PflMI PmlI PshAI PspOMI PspXI PsrI SacII
SanDI SfiI SgrAI SrfI StuI SwaI XhoI XmnI
ZraI