Restriction map for
pF1KSDB0068
(Circular) (Six-base) MAPSORT of: pF1KSDB0068.seq Check: 3336 from: 1 to: 6235
pF1KSDB0068 6235 bp
With 182 enzymes: SgfI *
June 20, 2008 10:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1550 1550
Size: 6235
AatII G_ACGT'C
Cuts at: 2540 2540
Size: 6235
AccI GT'mk_AC
Cuts at: 3271 3271
Size: 6235
Acc65I G'GTAC_C
Cuts at: 2801 3249 2801
Size: 448 5787
AclI AA'CG_TT
Cuts at: 6075 6075
Size: 6235
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2191 2344 2401 2782 4058 4490 5239 5507 2191
Size: 153 57 381 1276 432 749 268 2919
Fragments arranged by size:
2919 1276 749 432 381 268 153 57
AflIII A'CryG_T
Cuts at: 3585 4691 5668 3585
Size: 1106 977 4152
Fragments arranged by size:
4152 1106 977
AgeI A'CCGG_T
Cuts at: 4623 4623
Size: 6235
AhdI GACnn_n'nnGTC
Cuts at: 1626 1626
Size: 6235
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 175 2428 175
Size: 2253 3982
AlwNI CAG_nnn'CTG
Cuts at: 1596 1668 2421 3538 5107 1596
Size: 72 753 1117 1569 2724
Fragments arranged by size:
2724 1569 1117 753 72
ApaLI G'TGCA_C
Cuts at: 592 2756 5005 592
Size: 2164 2249 1822
Fragments arranged by size:
2249 2164 1822
ApoI r'AATT_y
Cuts at: 536 551 1125 3237 5588 5844 536
Size: 15 574 2112 2351 256 927
Fragments arranged by size:
2351 2112 927 574 256 15
AseI AT'TA_AT
Cuts at: 20 20
Size: 6235
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6235
AvaI C'yCGr_G
Cuts at: 464 1000 1357 1502 2489 2546 2850 3253 464
Size: 536 357 145 987 57 304 403 3446
Fragments arranged by size:
3446 987 536 403 357 304 145 57
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3033 3066 3033
Size: 33 6202
BamHI G'GATC_C
Cuts at: 3258 3258
Size: 6235
BanI G'GyrC_C
Cuts at: 364 612 1937 2090 2779 2801 2935 3249 3663
Size: 248 1325 153 689 22 134 314 414
Cuts at: 3663 3911 3946 364
Size: 248 35 2653
Fragments arranged by size:
2653 1325 689 414 314 248 248 153
134 35 22
BanII G_rGCy'C
Cuts at: 1304 2003 2318 2576 2851 2947 3247 4277 1304
Size: 699 315 258 275 96 300 1030 3262
Fragments arranged by size:
3262 1030 699 315 300 275 258 96
BbeI G_GCGC'C
Cuts at: 2783 3667 3915 2783
Size: 884 248 5103
Fragments arranged by size:
5103 884 248
BbsI GAAGACnn'nnnn_
Cuts at: 1186 2308 2585 1186
Size: 1122 277 4836
Fragments arranged by size:
4836 1122 277
BbvCI CC'TCA_GC
Cuts at: 434 935 1769 3173 434
Size: 501 834 1404 3496
Fragments arranged by size:
3496 1404 834 501
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2508 2542 2508
Size: 34 6201
BciVI GTATCCnnnnn_n'
Cuts at: 4131 4894 6235 4131
Size: 763 1341 4131
Fragments arranged by size:
4131 1341 763
BclI T'GATC_A
Cuts at: 3753 3753
Size: 6235
BfrBI ATG'CAT
Cuts at: 319 319
Size: 6235
BglII A'GATC_T
Cuts at: 2682 3748 5787 2682
Size: 1066 2039 3130
Fragments arranged by size:
3130 2039 1066
BlpI GC'TnA_GC
Cuts at: 1142 1220 1225 1534 3345 1142
Size: 78 5 309 1811 4032
Fragments arranged by size:
4032 1811 309 78 5
Bme1580I G_kGCm'C
Cuts at: 596 943 2760 3858 3951 5009 596
Size: 347 1817 1098 93 1058 1822
Fragments arranged by size:
1822 1817 1098 1058 347 93
BmrI ACTGGGnnnn_n'
Cuts at: 3445 3526 3861 6007 3445
Size: 81 335 2146 3673
Fragments arranged by size:
3673 2146 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1318 4600 1318
Size: 3282 2953
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 872 973 1234 1618 1732 1942 1969 2122 2242
Size: 101 261 384 114 210 27 153 120
Cuts at: 2242 2323 2482 2494 2770 872
Size: 81 159 12 276 4337
Fragments arranged by size:
4337 384 276 261 210 159 153 120
114 101 81 27 12
Bpu10I CC'TnA_GC
Cuts at: 434 935 1565 1769 3173 434
Size: 501 630 204 1404 3496
Fragments arranged by size:
3496 1404 630 501 204
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 284 526 1444 2136 2449 2644 3409 4288 4782
Size: 242 918 692 313 195 765 879 494
Cuts at: 4782 5080 284
Size: 298 1439
Fragments arranged by size:
1439 918 879 765 692 494 313 298
242 195
BsaI GGTCTCn'nnnn_
Cuts at: 127 1057 1306 5939 127
Size: 930 249 4633 423
Fragments arranged by size:
4633 930 423 249
BsaAI yAC'GTr
Cuts at: 2671 3225 3586 2671
Size: 554 361 5320
Fragments arranged by size:
5320 554 361
BsaBI GATnn'nnATC
Cuts at: 6 445 3747 5786 6
Size: 439 3302 2039 455
Fragments arranged by size:
3302 2039 455 439
BsaHI Gr'CG_yC
Cuts at: 2537 2780 3664 3912 4616 6112 2537
Size: 243 884 248 704 1496 2660
Fragments arranged by size:
2660 1496 884 704 248 243
BsaWI w'CCGG_w
Cuts at: 3422 3943 4623 4897 5044 5455 3422
Size: 521 680 274 147 411 4202
Fragments arranged by size:
4202 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 472 502 1235 1265 1705 1735 472
Size: 30 733 30 440 30 4972
Fragments arranged by size:
4972 733 440 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 673 1294 1324 1375 1393 1396 1520 1615 1657
Size: 621 30 51 18 3 124 95 42
Cuts at: 1657 1882 1885 2008 2491 673
Size: 225 3 123 483 4417
Fragments arranged by size:
4417 621 483 225 124 123 95 51
42 30 18 3 3
BseYI C'CCAG_C
Cuts at: 1273 2907 3013 3658 4416 4995 5491 1273
Size: 1634 106 645 758 579 496 2017
Fragments arranged by size:
2017 1634 758 645 579 496 106
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 409 2582 409
Size: 2173 4062
BsiEI CG_ry'CG
Cuts at: 104 3775 3821 5031 104
Size: 3671 46 1210 1308
Fragments arranged by size:
3671 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 164 596 1304 2003 2318 2407 2760 2851 3247
Size: 432 708 699 315 89 353 91 396
Cuts at: 3247 4025 4215 5009 164
Size: 778 190 794 1390
Fragments arranged by size:
1390 794 778 708 699 432 396 353
315 190 91 89
BsmBI CGTCTCn'nnnn_
Cuts at: 1192 1399 1192
Size: 207 6028
Bsp1286I G_dGCh'C
Cuts at: 164 596 943 1304 2003 2318 2407 2576 2760
Size: 432 347 361 699 315 89 169 184
Cuts at: 2760 2851 2947 3247 3858 3951 4025 4215 4277
Size: 91 96 300 611 93 74 190 62
Cuts at: 4277 5009 164
Size: 732 1390
Fragments arranged by size:
1390 732 699 611 432 361 347 315
300 190 184 169 96 93 91 89
74 62
BspEI T'CCGG_A
Cuts at: 5455 5455
Size: 6235
BspHI T'CATG_A
Cuts at: 5411 5411
Size: 6235
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1550 1581 3008 3161 3283 3497 3799 4180 1550
Size: 31 1427 153 122 214 302 381 3605
Fragments arranged by size:
3605 1427 381 302 214 153 122 31
BsrBI CCG'CTC
Cuts at: 50 1365 2048 4525 4579 6065 6230 50
Size: 1315 683 2477 54 1486 165 55
Fragments arranged by size:
2477 1486 1315 683 165 55 54
BsrDI GCAATG_nn'
Cuts at: 921 1245 4145 921
Size: 324 2900 3011
Fragments arranged by size:
3011 2900 324
BsrFI r'CCGG_y
Cuts at: 1229 1940 2258 2798 4231 4412 4623 1229
Size: 711 318 540 1433 181 211 2841
Fragments arranged by size:
2841 1433 711 540 318 211 181
BssSI C'ACGA_G
Cuts at: 595 2395 4504 4864 595
Size: 1800 2109 360 1966
Fragments arranged by size:
2109 1966 1800 360
BstAPI GCAn_nnn'nTGC
Cuts at: 1470 3610 1470
Size: 2140 4095
BstBI TT'CG_AA
Cuts at: 110 110
Size: 6235
BstYI r'GATC_y
Cuts at: 1 1385 1794 2042 2682 3258 3725 3748 4083
Size: 1384 409 248 640 576 467 23 335
Cuts at: 4083 4329 5343 5429 5441 5787 1
Size: 246 1014 86 12 346 449
Fragments arranged by size:
1384 1014 640 576 467 449 409 346
335 248 246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 393 393
Size: 6235
BtgI C'CryG_G
Cuts at: 125 410 491 125
Size: 285 81 5869
Fragments arranged by size:
5869 285 81
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1335 2135 4183 4364 4527 1335
Size: 800 2048 181 163 3043
Fragments arranged by size:
3043 2048 800 181 163
BtsI GCAGTG_nn'
Cuts at: 1112 1153 3063 3476 1112
Size: 41 1910 413 3871
Fragments arranged by size:
3871 1910 413 41
DraI TTT'AAA
Cuts at: 3232 3232
Size: 6235
DrdI GACnn_nn'nnGTC
Cuts at: 241 585 3939 4799 241
Size: 344 3354 860 1677
Fragments arranged by size:
3354 1677 860 344
EaeI y'GGCC_r
Cuts at: 1694 2129 2898 3818 3992 4383 4410 5757 1694
Size: 435 769 920 174 391 27 1347 2172
Fragments arranged by size:
2172 1347 920 769 435 391 174 27
EagI C'GGCC_G
Cuts at: 3818 3818
Size: 6235
EarI CTCTTCn'nnn_
Cuts at: 1478 1642 2093 2377 2395 2980 4256 4466 1478
Size: 164 451 284 18 585 1276 210 3247
Fragments arranged by size:
3247 1276 585 451 284 210 164 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 145 1378 4441 4753 4899 6045 145
Size: 1233 3063 312 146 1146 335
Fragments arranged by size:
3063 1233 1146 335 312 146
EcoICRI GAG'CTC
Cuts at: 1302 2001 2316 2849 3245 1302
Size: 699 315 533 396 4292
Fragments arranged by size:
4292 699 533 396 315
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 872 973 1234 1618 1732 1942 1969 2122 2191
Size: 101 261 384 114 210 27 153 69
Cuts at: 2191 2242 2323 2344 2401 2482 2494 2770 2782
Size: 51 81 21 57 81 12 276 12
Cuts at: 2782 4058 4490 5239 5507 872
Size: 1276 432 749 268 1600
Fragments arranged by size:
1600 1276 749 432 384 276 268 261
210 153 114 101 81 81 69 57
51 27 21 12 12
EcoNI CCTnn'n_nnAGG
Cuts at: 184 184
Size: 6235
EcoO109I rG'GnC_Cy
Cuts at: 1593 2460 2745 3372 1593
Size: 867 285 627 4456
Fragments arranged by size:
4456 867 627 285
EcoRI G'AATT_C
Cuts at: 3237 3237
Size: 6235
EcoRV GAT'ATC
Cuts at: 3132 3132
Size: 6235
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 364 2634 4252 364
Size: 2270 1618 2347
Fragments arranged by size:
2347 2270 1618
FspI TGC'GCA
Cuts at: 1348 4014 1348
Size: 2666 3569
FspAI rTGC'GCAy
Cuts at: 1348 1348
Size: 6235
HaeII r_GCGC'y
Cuts at: 1269 2783 2799 3667 3915 4939 5918 1269
Size: 1514 16 868 248 1024 979 1586
Fragments arranged by size:
1586 1514 1024 979 868 248 16
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1054 1086 1318 1350 1377 1409 1612 1644 1666
Size: 32 232 32 27 32 203 32 22
Cuts at: 1666 1698 1779 1811 1880 1912 1990 2022 2223
Size: 32 81 32 69 32 78 32 201
Cuts at: 2223 2255 4075 4105 4107 4137 1054
Size: 32 1820 30 2 30 3152
Fragments arranged by size:
3152 1820 232 203 201 81 78 69
32 32 32 32 32 32 32 32
32 30 30 27 22 2
HincII GTy'rAC
Cuts at: 1136 3272 1136
Size: 2136 4099
HindIII A'AGCT_T
Cuts at: 2356 3187 2356
Size: 831 5404
KasI G'GCGC_C
Cuts at: 2779 3663 3911 2779
Size: 884 248 5103
Fragments arranged by size:
5103 884 248
KpnI G_GTAC'C
Cuts at: 2805 3253 2805
Size: 448 5787
MluI A'CGCG_T
Cuts at: 5668 5668
Size: 6235
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4906 5090 4906
Size: 184 6051
MscI TGG'CCA
Cuts at: 1696 2131 2900 3994 1696
Size: 435 769 1094 3937
Fragments arranged by size:
3937 1094 769 435
MslI CAynn'nnrTG
Cuts at: 157 320 715 1078 1342 4222 4349 157
Size: 163 395 363 264 2880 127 2043
Fragments arranged by size:
2880 2043 395 363 264 163 127
MspA1I CmG'CkG
Cuts at: 438 1092 1527 1728 1947 2265 2664 3344 3658
Size: 654 435 201 219 318 399 680 314
Cuts at: 3658 4018 5033 5278 438
Size: 360 1015 245 1395
Fragments arranged by size:
1395 1015 680 654 435 399 360 318
314 245 219 201
NaeI GCC'GGC
Cuts at: 1942 4414 1942
Size: 2472 3763
NarI GG'CG_CC
Cuts at: 2780 3664 3912 2780
Size: 884 248 5103
Fragments arranged by size:
5103 884 248
NcoI C'CATG_G
Cuts at: 125 410 491 125
Size: 285 81 5869
Fragments arranged by size:
5869 285 81
NdeI CA'TA_TG
Cuts at: 264 264
Size: 6235
NgoMIV G'CCGG_C
Cuts at: 1940 4412 1940
Size: 2472 3763
NruI TCG'CGA
Cuts at: 4596 4596
Size: 6235
NsiI A_TGCA'T
Cuts at: 321 321
Size: 6235
NspI r_CATG'y
Cuts at: 323 2067 3286 3786 4695 323
Size: 1744 1219 500 909 1863
Fragments arranged by size:
1863 1744 1219 909 500
PasI CC'CwG_GG
Cuts at: 1290 1290
Size: 6235
PciI A'CATG_T
Cuts at: 4691 4691
Size: 6235
PflMI CCAn_nnn'nTGG
Cuts at: 1638 1668 1638
Size: 30 6205
PfoI T'CCnGG_A
Cuts at: 989 1595 2276 989
Size: 606 681 4948
Fragments arranged by size:
4948 681 606
PmeI GTTT'AAAC
Cuts at: 3232 3232
Size: 6235
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1102 1134 1858 1890 5374 5406 1102
Size: 32 724 32 3484 32 1931
Fragments arranged by size:
3484 1931 724 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 1593 2460 1593
Size: 867 5368
PshAI GACnn'nnGTC
Cuts at: 2548 2548
Size: 6235
PsiI TTA'TAA
Cuts at: 1052 1052
Size: 6235
PspXI vC'TCGA_Gb
Cuts at: 2546 2546
Size: 6235
PstI C_TGCA'G
Cuts at: 1592 2231 2417 3005 3280 3965 1592
Size: 639 186 588 275 685 3862
Fragments arranged by size:
3862 685 639 588 275 186
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6235
PvuII CAG'CTG
Cuts at: 438 1947 2265 2664 3658 4018 438
Size: 1509 318 399 994 360 2655
Fragments arranged by size:
2655 1509 994 399 360 318
RsrII CG'GwC_CG
Cuts at: 4428 4428
Size: 6235
SacI G_AGCT'C
Cuts at: 1304 2003 2318 2851 3247 1304
Size: 699 315 533 396 4292
Fragments arranged by size:
4292 699 533 396 315
SalI G'TCGA_C
Cuts at: 3270 3270
Size: 6235
SapI GCTCTTCn'nnn_
Cuts at: 1478 1642 2377 2395 4256 4466 1478
Size: 164 735 18 1861 210 3247
Fragments arranged by size:
3247 1861 735 210 164 18
SbfI CC_TGCA'GG
Cuts at: 3005 3280 3005
Size: 275 5960
ScaI AGT'ACT
Cuts at: 5752 5752
Size: 6235
SfcI C'TryA_G
Cuts at: 33 1588 1732 2227 2413 3001 3276 3961 4956
Size: 1555 144 495 186 588 275 685 995
Cuts at: 4956 5147 33
Size: 191 1121
Fragments arranged by size:
1555 1121 995 685 588 495 275 191
186 144
SfoI GGC'GCC
Cuts at: 2781 3665 3913 2781
Size: 884 248 5103
Fragments arranged by size:
5103 884 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6235
SmaI CCC'GGG
Cuts at: 2491 3255 2491
Size: 764 5471
SmlI C'TyrA_G
Cuts at: 299 505 1459 2115 2464 2546 2623 3388 4303
Size: 206 954 656 349 82 77 765 915
Cuts at: 4303 4797 5059 299
Size: 494 262 1475
Fragments arranged by size:
1475 954 915 765 656 494 349 262
206 82 77
SnaBI TAC'GTA
Cuts at: 3225 3225
Size: 6235
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6235
SphI G_CATG'C
Cuts at: 3286 3786 3286
Size: 500 5735
StyI C'CwwG_G
Cuts at: 125 228 410 491 2771 3083 3367 3721 125
Size: 103 182 81 2280 312 284 354 2639
Fragments arranged by size:
2639 2280 354 312 284 182 103 81
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2934 3027 3816 3949 4408 4619 2934
Size: 93 789 133 459 211 4550
Fragments arranged by size:
4550 789 459 211 133 93
TatI w'GTAC_w
Cuts at: 631 3032 5750 631
Size: 2401 2718 1116
Fragments arranged by size:
2718 2401 1116
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 472 504 1235 1267 5752 5784 472
Size: 32 731 32 4485 32 923
Fragments arranged by size:
4485 923 731 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 241 1068 4030 241
Size: 827 2962 2446
Fragments arranged by size:
2962 2446 827
XbaI T'CTAG_A
Cuts at: 3264 3264
Size: 6235
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1707 3728 1707
Size: 2021 4214
XhoI C'TCGA_G
Cuts at: 2546 2546
Size: 6235
XmaI C'CCGG_G
Cuts at: 2489 3253 2489
Size: 764 5471
ZraI GAC'GTC
Cuts at: 2538 2538
Size: 6235
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AhdI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI
BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BfrBI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI FspAI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NruI NsiI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspXI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SnaBI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI ZraI
Enzymes that do not cut:
AfeI AflII AleI AloI ApaI AscI AvrII BglI
BmgBI BmtI BsiWI BsmI BsrGI BssHII BstEII BstXI
BstZ17I ClaI CspCI DraIII FseI HpaI MfeI NheI
NotI PacI PmlI PspOMI PsrI SacII SanDI SexAI
SfiI SgrAI SrfI SspI StuI SwaI XmnI