Restriction map for
pF1KSDB0185
(Circular) (Six-base) MAPSORT of: pF1KSDB0185.seq Check: 7519 from: 1 to: 8075
pF1KSDB0185 8075 bp
With 182 enzymes: SgfI *
June 20, 2008 10:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1293 4913 5042 1293
Size: 3620 129 4326
Fragments arranged by size:
4326 3620 129
AccI GT'mk_AC
Cuts at: 5113 5113
Size: 8075
Acc65I G'GTAC_C
Cuts at: 5091 5091
Size: 8075
AclI AA'CG_TT
Cuts at: 7915 7915
Size: 8075
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1015 1188 1212 1870 1898 2056 2710 2964 3021
Size: 173 24 658 28 158 654 254 57
Cuts at: 3021 3922 5900 6332 7079 7347 1015
Size: 901 1978 432 747 268 1743
Fragments arranged by size:
1978 1743 901 747 658 654 432 268
254 173 158 57 28 24
AfeI AGC'GCT
Cuts at: 2319 2319
Size: 8075
AflIII A'CryG_T
Cuts at: 614 3692 5427 6531 7508 614
Size: 3078 1735 1104 977 1181
Fragments arranged by size:
3078 1735 1181 1104 977
AgeI A'CCGG_T
Cuts at: 6463 6463
Size: 8075
AhdI GACnn_n'nnGTC
Cuts at: 4572 4572
Size: 8075
AleI CACnn'nnGTG
Cuts at: 1040 1655 2098 1040
Size: 615 443 7017
Fragments arranged by size:
7017 615 443
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1048 1058 2116 4361 1048
Size: 10 1058 2245 4762
Fragments arranged by size:
4762 2245 1058 10
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3679 3711 5000 5032 3679
Size: 32 1289 32 6722
Fragments arranged by size:
6722 1289 32 32
AlwNI CAG_nnn'CTG
Cuts at: 318 837 1028 1308 1896 2552 2742 2895 3106
Size: 519 191 280 588 656 190 153 211
Cuts at: 3106 3374 5380 6947 318
Size: 268 2006 1567 1446
Fragments arranged by size:
2006 1567 1446 656 588 519 280 268
211 191 190 153
ApaLI G'TGCA_C
Cuts at: 4692 6845 4692
Size: 2153 5922
ApoI r'AATT_y
Cuts at: 1739 5079 7428 7684 1739
Size: 3340 2349 256 2130
Fragments arranged by size:
3340 2349 2130 256
AscI GG'CGCG_CC
Cuts at: 2541 2541
Size: 8075
AseI AT'TA_AT
Cuts at: 20 20
Size: 8075
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 8075
AvaI C'yCGr_G
Cuts at: 297 1480 2028 2869 3852 4631 5095 297
Size: 1183 548 841 983 779 464 3277
Fragments arranged by size:
3277 1183 983 841 779 548 464
BamHI G'GATC_C
Cuts at: 236 5100 236
Size: 4864 3211
BanI G'GyrC_C
Cuts at: 1657 2215 2745 3017 4131 4601 5091 5505 5753
Size: 558 530 272 1114 470 490 414 248
Cuts at: 5753 5788 1657
Size: 35 3944
Fragments arranged by size:
3944 1114 558 530 490 470 414 272
248 35
BanII G_rGCy'C
Cuts at: 560 1022 2024 2610 2654 2801 2942 4109 4632
Size: 462 1002 586 44 147 141 1167 523
Cuts at: 4632 5089 6119 560
Size: 457 1030 2516
Fragments arranged by size:
2516 1167 1030 1002 586 523 462 457
147 141 44
BbeI G_GCGC'C
Cuts at: 4605 5509 5757 4605
Size: 904 248 6923
Fragments arranged by size:
6923 904 248
BbsI GAAGACnn'nnnn_
Cuts at: 519 1362 1475 1672 2270 2448 4222 5007 519
Size: 843 113 197 598 178 1774 785 3587
Fragments arranged by size:
3587 1774 843 785 598 197 178 113
BbvCI CC'TCA_GC
Cuts at: 1394 2818 2927 4921 1394
Size: 1424 109 1994 4548
Fragments arranged by size:
4548 1994 1424 109
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2832 2866 3442 3476 4512 4546 2832
Size: 34 576 34 1036 34 6361
Fragments arranged by size:
6361 1036 576 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5973 6734 8075 5973
Size: 761 1341 5973
Fragments arranged by size:
5973 1341 761
BclI T'GATC_A
Cuts at: 431 5595 431
Size: 5164 2911
BglI GCCn_nnn'nGGC
Cuts at: 2158 2776 3205 3328 3880 4196 2158
Size: 618 429 123 552 316 6037
Fragments arranged by size:
6037 618 552 429 316 123
BglII A'GATC_T
Cuts at: 4997 5590 7627 4997
Size: 593 2037 5445
Fragments arranged by size:
5445 2037 593
BlpI GC'TnA_GC
Cuts at: 931 1021 4519 5187 931
Size: 90 3498 668 3819
Fragments arranged by size:
3819 3498 668 90
Bme1580I G_kGCm'C
Cuts at: 281 426 1786 1876 2297 2858 4136 4696 5700
Size: 145 1360 90 421 561 1278 560 1004
Cuts at: 5700 5793 6849 281
Size: 93 1056 1507
Fragments arranged by size:
1507 1360 1278 1056 1004 561 560 421
145 93 90
BmgBI CAC'GTC
Cuts at: 1864 3456 1864
Size: 1592 6483
BmrI ACTGGGnnnn_n'
Cuts at: 2562 2657 3413 5287 5368 5703 7847 2562
Size: 95 756 1874 81 335 2144 2790
Fragments arranged by size:
2790 2144 1874 756 335 95 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2043 6442 2043
Size: 4399 3676
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1059 1933 2031 2329 2410 2983 3083 3864 4483
Size: 874 98 298 81 573 100 781 619
Cuts at: 4483 4843 4969 1059
Size: 360 126 4165
Fragments arranged by size:
4165 874 781 619 573 360 298 126
100 98 81
Bpu10I CC'TnA_GC
Cuts at: 136 1394 1945 2818 2927 4412 4921 5020 136
Size: 1258 551 873 109 1485 509 99 3191
Fragments arranged by size:
3191 1485 1258 873 551 509 109 99
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2185 4738 5251 6130 6622 6920 2185
Size: 2553 513 879 492 298 3340
Fragments arranged by size:
3340 2553 879 513 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1250 1595 1699 2135 2380 3177 3919 7779 1250
Size: 345 104 436 245 797 742 3860 1546
Fragments arranged by size:
3860 1546 797 742 436 345 245 104
BsaAI yAC'GTr
Cuts at: 2292 3363 3693 5067 5428 2292
Size: 1071 330 1374 361 4939
Fragments arranged by size:
4939 1374 1071 361 330
BsaBI GATnn'nnATC
Cuts at: 6 241 1543 5589 7626 6
Size: 235 1302 4046 2037 455
Fragments arranged by size:
4046 2037 1302 455 235
BsaHI Gr'CG_yC
Cuts at: 2039 4602 5506 5754 6456 7952 2039
Size: 2563 904 248 702 1496 2162
Fragments arranged by size:
2563 2162 1496 904 702 248
BsaWI w'CCGG_w
Cuts at: 1763 3968 5264 5785 6463 6737 6884 7295 1763
Size: 2205 1296 521 678 274 147 411 2543
Fragments arranged by size:
2543 2205 1296 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 324 354 1906 1936 2029 2059 2161 2191 3347
Size: 30 1552 30 93 30 102 30 1156
Cuts at: 3347 3377 3388 3418 3760 3790 4141 4171 4969
Size: 30 11 30 342 30 351 30 798
Cuts at: 4969 4999 324
Size: 30 3400
Fragments arranged by size:
3400 1552 1156 798 351 342 102 93
30 30 30 30 30 30 30 30
30 11
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 142 145 1063 2632 2635 2781 2980 3768 3987
Size: 3 918 1569 3 146 199 788 219
Cuts at: 3987 4261 4690 4840 5068 142
Size: 274 429 150 228 3149
Fragments arranged by size:
3149 1569 918 788 429 274 228 219
199 150 146 3 3
BseYI C'CCAG_C
Cuts at: 1308 1644 1844 2136 2273 2320 2409 2879 2883
Size: 336 200 292 137 47 89 470 4
Cuts at: 2883 3185 3518 3651 4063 4194 4745 4925 5500
Size: 302 333 133 412 131 551 180 575
Cuts at: 5500 6258 6835 7331 1308
Size: 758 577 496 2052
Fragments arranged by size:
2052 758 577 575 551 496 470 412
336 333 302 292 200 180 137 133
131 89 47 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 787 994 1018 1882 2644 3346 3361 3756 4189
Size: 207 24 864 762 702 15 395 433
Cuts at: 4189 4735 4816 787
Size: 546 81 4046
Fragments arranged by size:
4046 864 762 702 546 433 395 207
81 24 15
BsiEI CG_ry'CG
Cuts at: 104 1595 3062 3864 5617 5663 6871 104
Size: 1491 1467 802 1753 46 1208 1308
Fragments arranged by size:
1753 1491 1467 1308 1208 802 46
BsiHKAI G_wGCw'C
Cuts at: 137 536 1166 1337 1829 2359 2801 2954 4109
Size: 399 630 171 492 530 442 153 1155
Cuts at: 4109 4525 4696 4883 5089 5867 6057 6849 137
Size: 416 171 187 206 778 190 792 1363
Fragments arranged by size:
1363 1155 792 778 630 530 492 442
416 399 206 190 187 171 171 153
BsmI GAATG_Cn'
Cuts at: 3277 3277
Size: 8075
BsmBI CGTCTCn'nnnn_
Cuts at: 3751 3751
Size: 8075
Bsp1286I G_dGCh'C
Cuts at: 137 281 426 536 560 1022 1166 1337 1786
Size: 144 145 110 24 462 144 171 449
Cuts at: 1786 1829 1876 2024 2297 2359 2610 2654 2801
Size: 43 47 148 273 62 251 44 147
Cuts at: 2801 2858 2942 2954 4109 4136 4525 4632 4696
Size: 57 84 12 1155 27 389 107 64
Cuts at: 4696 4883 5089 5700 5793 5867 6057 6119 6849
Size: 187 206 611 93 74 190 62 730
Cuts at: 6849 137
Size: 1363
Fragments arranged by size:
1363 1155 730 611 462 449 389 273
251 206 190 187 171 148 147 145
144 144 110 107 93 84 74 64
62 62 57 47 44 43 27 24
12
BspEI T'CCGG_A
Cuts at: 1763 3968 7295 1763
Size: 2205 3327 2543
Fragments arranged by size:
3327 2543 2205
BspHI T'CATG_A
Cuts at: 1091 7251 1091
Size: 6160 1915
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1293 2141 2764 3269 3941 4913 5042 5125 5339
Size: 848 623 505 672 972 129 83 214
Cuts at: 5339 5641 6022 1293
Size: 302 381 3346
Fragments arranged by size:
3346 972 848 672 623 505 381 302
214 129 83
BsrBI CCG'CTC
Cuts at: 50 1949 3015 6367 6421 7905 8070 50
Size: 1899 1066 3352 54 1484 165 55
Fragments arranged by size:
3352 1899 1484 1066 165 55 54
BsrDI GCAATG_nn'
Cuts at: 220 956 2414 3248 5987 220
Size: 736 1458 834 2739 2308
Fragments arranged by size:
2739 2308 1458 834 736
BsrFI r'CCGG_y
Cuts at: 2218 3863 4525 6073 6254 6463 2218
Size: 1645 662 1548 181 209 3830
Fragments arranged by size:
3830 1645 1548 662 209 181
BssHII G'CGCG_C
Cuts at: 2541 2541
Size: 8075
BssSI C'ACGA_G
Cuts at: 892 2533 3582 4625 6346 6704 892
Size: 1641 1049 1043 1721 358 2263
Fragments arranged by size:
2263 1721 1641 1049 1043 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1896 3959 4131 5452 1896
Size: 2063 172 1321 4519
Fragments arranged by size:
4519 2063 1321 172
BstBI TT'CG_AA
Cuts at: 110 6436 110
Size: 6326 1749
BstEII G'GTnAC_C
Cuts at: 843 1347 2956 3633 843
Size: 504 1609 677 5285
Fragments arranged by size:
5285 1609 677 504
BstXI CCAn_nnnn'nTGG
Cuts at: 513 3111 3684 4482 4584 513
Size: 2598 573 798 102 4004
Fragments arranged by size:
4004 2598 798 573 102
BstYI r'GATC_y
Cuts at: 1 236 1760 2064 4997 5100 5567 5590 5925
Size: 235 1524 304 2933 103 467 23 335
Cuts at: 5925 6171 7183 7269 7281 7627 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
2933 1524 1012 467 449 346 335 304
246 235 103 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 3888 4019 3888
Size: 131 7944
BtgI C'CryG_G
Cuts at: 125 521 2444 2582 3272 3878 4145 4592 4619
Size: 396 1923 138 690 606 267 447 27
Cuts at: 4619 125
Size: 3581
Fragments arranged by size:
3581 1923 690 606 447 396 267 138
27
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 861 1062 1965 1974 2133 3600 4515 6025 6206
Size: 201 903 9 159 1467 915 1510 181
Cuts at: 6206 6369 861
Size: 163 2567
Fragments arranged by size:
2567 1510 1467 915 903 201 181 163
159 9
BtsI GCAGTG_nn'
Cuts at: 2182 2250 2661 2917 3104 3572 5318 2182
Size: 68 411 256 187 468 1746 4939
Fragments arranged by size:
4939 1746 468 411 256 187 68
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 2556 2591 2556
Size: 35 8040
DraI TTT'AAA
Cuts at: 5074 5074
Size: 8075
DraIII CAC_nnn'GTG
Cuts at: 2292 2292
Size: 8075
DrdI GACnn_nn'nnGTC
Cuts at: 5781 6639 5781
Size: 858 7217
EaeI y'GGCC_r
Cuts at: 515 761 896 1592 2159 2777 3113 3284 3365
Size: 246 135 696 567 618 336 171 81
Cuts at: 3365 3477 3569 3861 3881 4142 4703 5660 5834
Size: 112 92 292 20 261 561 957 174
Cuts at: 5834 6225 6252 7597 515
Size: 391 27 1345 993
Fragments arranged by size:
1345 993 957 696 618 567 561 391
336 292 261 246 174 171 135 112
92 81 27 20
EagI C'GGCC_G
Cuts at: 1592 3861 5660 1592
Size: 2269 1799 4007
Fragments arranged by size:
4007 2269 1799
EarI CTCTTCn'nnn_
Cuts at: 217 1072 1108 1762 2806 3271 3919 6098 6308
Size: 855 36 654 1044 465 648 2179 210
Cuts at: 6308 217
Size: 1984
Fragments arranged by size:
2179 1984 1044 855 654 648 465 210
36
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 332 743 3993 6283 6593 6739 7885 332
Size: 411 3250 2290 310 146 1146 522
Fragments arranged by size:
3250 2290 1146 522 411 310 146
EcoICRI GAG'CTC
Cuts at: 2799 4107 5087 2799
Size: 1308 980 5787
Fragments arranged by size:
5787 1308 980
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1015 1059 1188 1212 1870 1898 1933 2031 2056
Size: 44 129 24 658 28 35 98 25
Cuts at: 2056 2329 2410 2710 2964 2983 3021 3083 3864
Size: 273 81 300 254 19 38 62 781
Cuts at: 3864 3922 4483 4843 4969 5900 6332 7079 7347
Size: 58 561 360 126 931 432 747 268
Cuts at: 7347 1015
Size: 1743
Fragments arranged by size:
1743 931 781 747 658 561 432 360
300 273 268 254 129 126 98 81
62 58 44 38 35 28 25 24
19
EcoNI CCTnn'n_nnAGG
Cuts at: 344 1558 1621 2614 2692 3582 344
Size: 1214 63 993 78 890 4837
Fragments arranged by size:
4837 1214 993 890 78 63
EcoO109I rG'GnC_Cy
Cuts at: 189 1524 1854 2186 2739 2873 3396 4046 4427
Size: 1335 330 332 553 134 523 650 381
Cuts at: 4427 4452 4686 5214 189
Size: 25 234 528 3050
Fragments arranged by size:
3050 1335 650 553 528 523 381 332
330 234 134 25
EcoRI G'AATT_C
Cuts at: 5079 5079
Size: 8075
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2476 6094 2476
Size: 3618 4457
FspI TGC'GCA
Cuts at: 418 1009 3646 5856 418
Size: 591 2637 2210 2637
Fragments arranged by size:
2637 2637 2210 591
FspAI rTGC'GCAy
Cuts at: 1009 3646 1009
Size: 2637 5438
HaeII r_GCGC'y
Cuts at: 151 2321 3200 3851 4419 4605 4822 5509 5757
Size: 2170 879 651 568 186 217 687 248
Cuts at: 5757 6779 7758 151
Size: 1022 979 468
Fragments arranged by size:
2170 1022 979 879 687 651 568 468
248 217 186
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 484 516 2029 2061 2132 2164 2597 2629 3388
Size: 32 1513 32 71 32 433 32 759
Cuts at: 3388 3420 4141 4173 4558 4590 4969 5001 5917
Size: 32 721 32 385 32 379 32 916
Cuts at: 5917 5947 5949 5979 484
Size: 30 2 30 2580
Fragments arranged by size:
2580 1513 916 759 721 433 385 379
71 32 32 32 32 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1275 3066 5114 1275
Size: 1791 2048 4236
Fragments arranged by size:
4236 2048 1791
KasI G'GCGC_C
Cuts at: 4601 5505 5753 4601
Size: 904 248 6923
Fragments arranged by size:
6923 904 248
KpnI G_GTAC'C
Cuts at: 5095 5095
Size: 8075
MluI A'CGCG_T
Cuts at: 7508 7508
Size: 8075
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 248 2618 3200 4284 6746 6930 248
Size: 2370 582 1084 2462 184 1393
Fragments arranged by size:
2462 2370 1393 1084 582 184
MscI TGG'CCA
Cuts at: 898 2161 2779 3286 3367 4705 5836 898
Size: 1263 618 507 81 1338 1131 3137
Fragments arranged by size:
3137 1338 1263 1131 618 507 81
MslI CAynn'nnrTG
Cuts at: 130 442 806 1009 1040 1655 2098 2443 3640
Size: 312 364 203 31 615 443 345 1197
Cuts at: 3640 4003 4582 4711 6064 6191 130
Size: 363 579 129 1353 127 2014
Fragments arranged by size:
2014 1353 1197 615 579 443 364 363
345 312 203 129 127 31
MspA1I CmG'CkG
Cuts at: 144 567 825 912 1028 1730 1848 2076 2277
Size: 423 258 87 116 702 118 228 201
Cuts at: 2277 2632 2708 2883 2895 3655 4128 4320 4772
Size: 355 76 175 12 760 473 192 452
Cuts at: 4772 4925 5186 5500 5860 6873 7118 144
Size: 153 261 314 360 1013 245 1101
Fragments arranged by size:
1101 1013 760 702 473 452 423 360
355 314 261 258 245 228 201 192
175 153 118 116 87 76 12
NaeI GCC'GGC
Cuts at: 2220 6256 2220
Size: 4036 4039
NarI GG'CG_CC
Cuts at: 4602 5506 5754 4602
Size: 904 248 6923
Fragments arranged by size:
6923 904 248
NcoI C'CATG_G
Cuts at: 125 2444 2582 3272 3878 125
Size: 2319 138 690 606 4322
Fragments arranged by size:
4322 2319 690 606 138
NgoMIV G'CCGG_C
Cuts at: 2218 6254 2218
Size: 4036 4039
NspI r_CATG'y
Cuts at: 441 618 775 1008 3340 4474 5128 5628 6535
Size: 177 157 233 2332 1134 654 500 907
Cuts at: 6535 441
Size: 1981
Fragments arranged by size:
2332 1981 1134 907 654 500 233 177
157
PasI CC'CwG_GG
Cuts at: 1375 1375
Size: 8075
PciI A'CATG_T
Cuts at: 614 6531 614
Size: 5917 2158
PflMI CCAn_nnn'nTGG
Cuts at: 1752 2118 2552 3374 3720 4671 4890 1752
Size: 366 434 822 346 951 219 4937
Fragments arranged by size:
4937 951 822 434 366 346 219
PfoI T'CCnGG_A
Cuts at: 203 311 746 2047 4562 4868 203
Size: 108 435 1301 2515 306 3410
Fragments arranged by size:
3410 2515 1301 435 306 108
PmeI GTTT'AAAC
Cuts at: 5074 5074
Size: 8075
PmlI CAC'GTG
Cuts at: 2292 3363 3693 2292
Size: 1071 330 6674
Fragments arranged by size:
6674 1071 330
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7214 7246 7214
Size: 32 8043
PpuMI rG'GwC_Cy
Cuts at: 189 1524 2186 2739 2873 3396 4046 189
Size: 1335 662 553 134 523 650 4218
Fragments arranged by size:
4218 1335 662 650 553 523 134
PshAI GACnn'nnGTC
Cuts at: 2758 2758
Size: 8075
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 856 888 856
Size: 32 8043
PstI C_TGCA'G
Cuts at: 179 2177 2525 2924 3206 5039 5122 5807 179
Size: 1998 348 399 282 1833 83 685 2447
Fragments arranged by size:
2447 1998 1833 685 399 348 282 83
PvuI CG_AT'CG
Cuts at: 104 104
Size: 8075
PvuII CAG'CTG
Cuts at: 144 825 912 1028 1848 2277 2708 2883 2895
Size: 681 87 116 820 429 431 175 12
Cuts at: 2895 3655 4925 5500 5860 144
Size: 760 1270 575 360 2359
Fragments arranged by size:
2359 1270 820 760 681 575 431 429
360 175 116 87 12
RsrII CG'GwC_CG
Cuts at: 6270 6270
Size: 8075
SacI G_AGCT'C
Cuts at: 2801 4109 5089 2801
Size: 1308 980 5787
Fragments arranged by size:
5787 1308 980
SalI G'TCGA_C
Cuts at: 5112 5112
Size: 8075
SapI GCTCTTCn'nnn_
Cuts at: 1108 1762 2806 3271 3919 6098 6308 1108
Size: 654 1044 465 648 2179 210 2875
Fragments arranged by size:
2875 2179 1044 654 648 465 210
SbfI CC_TGCA'GG
Cuts at: 3206 5122 3206
Size: 1916 6159
ScaI AGT'ACT
Cuts at: 1504 7592 1504
Size: 6088 1987
SexAI A'CCwGG_T
Cuts at: 1351 1526 1351
Size: 175 7900
SfcI C'TryA_G
Cuts at: 33 175 2173 2521 2920 3202 5035 5118 5803
Size: 142 1998 348 399 282 1833 83 685
Cuts at: 5803 6796 6987 33
Size: 993 191 1121
Fragments arranged by size:
1998 1833 1121 993 685 399 348 282
191 142 83
SfiI GGCCn_nnn'nGGCC
Cuts at: 2776 3328 3880 2776
Size: 552 552 6971
Fragments arranged by size:
6971 552 552
SfoI GGC'GCC
Cuts at: 4603 5507 5755 4603
Size: 904 248 6923
Fragments arranged by size:
6923 904 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 8075
SmaI CCC'GGG
Cuts at: 299 2871 3854 5097 299
Size: 2572 983 1243 3277
Fragments arranged by size:
3277 2572 1243 983
SmlI C'TyrA_G
Cuts at: 2200 4753 5230 6145 6637 6899 2200
Size: 2553 477 915 492 262 3376
Fragments arranged by size:
3376 2553 915 492 477 262
SnaBI TAC'GTA
Cuts at: 5067 5067
Size: 8075
SpeI A'CTAG_T
Cuts at: 67 67
Size: 8075
SphI G_CATG'C
Cuts at: 775 5128 5628 775
Size: 4353 500 3222
Fragments arranged by size:
4353 3222 500
StuI AGG'CCT
Cuts at: 3448 4837 3448
Size: 1389 6686
StyI C'CwwG_G
Cuts at: 125 703 1441 1559 2000 2162 2444 2582 2828
Size: 578 738 118 441 162 282 138 246
Cuts at: 2828 3212 3272 3878 4112 4790 4884 4958 5209
Size: 384 60 606 234 678 94 74 251
Cuts at: 5209 5563 125
Size: 354 2637
Fragments arranged by size:
2637 738 678 606 578 441 384 354
282 251 246 234 162 138 118 94
74 60
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2125 5658 5791 6250 6459 2125
Size: 3533 133 459 209 3741
Fragments arranged by size:
3741 3533 459 209 133
TatI w'GTAC_w
Cuts at: 1001 1502 7590 1001
Size: 501 6088 1486
Fragments arranged by size:
6088 1486 501
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 324 356 655 687 1906 1938 2161 2193 3347
Size: 32 299 32 1219 32 223 32 1154
Cuts at: 3347 3379 4482 4514 7592 7624 324
Size: 32 1103 32 3078 32 775
Fragments arranged by size:
3078 1219 1154 1103 775 299 223 32
32 32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 5872 5872
Size: 8075
XbaI T'CTAG_A
Cuts at: 5106 5106
Size: 8075
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 353 2031 2115 2304 2442 3279 3627 3717 5570
Size: 1678 84 189 138 837 348 90 1853
Cuts at: 5570 353
Size: 2858
Fragments arranged by size:
2858 1853 1678 837 348 189 138 90
84
XmaI C'CCGG_G
Cuts at: 297 2869 3852 5095 297
Size: 2572 983 1243 3277
Fragments arranged by size:
3277 2572 1243 983
XmnI GAAnn'nnTTC
Cuts at: 3241 3553 4936 3241
Size: 312 1383 6380
Fragments arranged by size:
6380 1383 312
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AscI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsrI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SgfI SmaI SmlI SnaBI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AatII AflII ApaI AvrII BaeI BfrBI BmtI BsiWI
BsrGI BstZ17I ClaI EcoRV FseI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PsiI PspOMI
PspXI SacII SanDI SgrAI SrfI SspI SwaI XhoI
ZraI