Restriction map for
pF1KSDB0234
(Circular) (Six-base) MAPSORT of: pF1KSDB0234.seq Check: 2886 from: 1 to: 6746
pF1KSDB0234 6746 bp
With 182 enzymes: SgfI *
June 20, 2008 10:36 ..
AccI GT'mk_AC
Cuts at: 314 2269 3784 314
Size: 1955 1515 3276
Fragments arranged by size:
3276 1955 1515
Acc65I G'GTAC_C
Cuts at: 928 2402 3762 928
Size: 1474 1360 3912
Fragments arranged by size:
3912 1474 1360
AclI AA'CG_TT
Cuts at: 6586 6586
Size: 6746
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 477 3081 3353 3413 3700 4571 5003 5750 6018
Size: 2604 272 60 287 871 432 747 268
Cuts at: 6018 477
Size: 1205
Fragments arranged by size:
2604 1205 871 747 432 287 272 268
60
AflIII A'CryG_T
Cuts at: 4098 5202 6179 4098
Size: 1104 977 4665
Fragments arranged by size:
4665 1104 977
AgeI A'CCGG_T
Cuts at: 5134 5134
Size: 6746
AleI CACnn'nnGTG
Cuts at: 1306 2425 3098 1306
Size: 1119 673 4954
Fragments arranged by size:
4954 1119 673
AlwNI CAG_nnn'CTG
Cuts at: 631 1561 1753 1927 3569 4051 5618 631
Size: 930 192 174 1642 482 1567 1759
Fragments arranged by size:
1759 1642 1567 930 482 192 174
ApaI G_GGCC'C
Cuts at: 2890 3145 2890
Size: 255 6491
ApaLI G'TGCA_C
Cuts at: 3408 5516 3408
Size: 2108 4638
ApoI r'AATT_y
Cuts at: 377 1426 3298 3750 6099 6355 377
Size: 1049 1872 452 2349 256 768
Fragments arranged by size:
2349 1872 1049 768 452 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6746
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6746
AvaI C'yCGr_G
Cuts at: 1145 3380 3766 1145
Size: 2235 386 4125
Fragments arranged by size:
4125 2235 386
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 764 797 920 953 764
Size: 33 123 33 6557
Fragments arranged by size:
6557 123 33 33
BamHI G'GATC_C
Cuts at: 1986 3771 1986
Size: 1785 4961
BanI G'GyrC_C
Cuts at: 424 928 1000 1231 1417 2184 2402 2590 2683
Size: 504 72 231 186 767 218 188 93
Cuts at: 2683 2892 3762 4176 4424 4459 424
Size: 209 870 414 248 35 2711
Fragments arranged by size:
2711 870 767 504 414 248 231 218
209 188 186 93 72 35
BanII G_rGCy'C
Cuts at: 2890 3145 3760 4790 2890
Size: 255 615 1030 4846
Fragments arranged by size:
4846 1030 615 255
BbeI G_GCGC'C
Cuts at: 428 4180 4428 428
Size: 3752 248 2746
Fragments arranged by size:
3752 2746 248
BbsI GAAGACnn'nnnn_
Cuts at: 1619 3584 1619
Size: 1965 4781
BbvCI CC'TCA_GC
Cuts at: 437 437
Size: 6746
BciVI GTATCCnnnnn_n'
Cuts at: 369 519 4644 5405 6746 369
Size: 150 4125 761 1341 369
Fragments arranged by size:
4125 1341 761 369 150
BclI T'GATC_A
Cuts at: 500 593 4266 500
Size: 93 3673 2980
Fragments arranged by size:
3673 2980 93
BfrBI ATG'CAT
Cuts at: 2647 2647
Size: 6746
BglII A'GATC_T
Cuts at: 4261 6298 4261
Size: 2037 4709
BlpI GC'TnA_GC
Cuts at: 3858 3858
Size: 6746
Bme1580I G_kGCm'C
Cuts at: 242 1005 1420 2189 2890 3145 3412 3554 4371
Size: 763 415 769 701 255 267 142 817
Cuts at: 4371 4464 5520 242
Size: 93 1056 1468
Fragments arranged by size:
1468 1056 817 769 763 701 415 267
255 142 93
BmgBI CAC'GTC
Cuts at: 1576 1576
Size: 6746
BmrI ACTGGGnnnn_n'
Cuts at: 380 1061 1460 2137 2892 3958 4039 4374 6518
Size: 681 399 677 755 1066 81 335 2144
Cuts at: 6518 380
Size: 608
Fragments arranged by size:
2144 1066 755 681 677 608 399 335
81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 430 5113 430
Size: 4683 2063
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 695 1689 1949 2018 2875 2972 695
Size: 994 260 69 857 97 4469
Fragments arranged by size:
4469 994 857 260 97 69
Bpu10I CC'TnA_GC
Cuts at: 437 437
Size: 6746
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 179 484 1522 3398 3922 4801 5293 5591 179
Size: 305 1038 1876 524 879 492 298 1334
Fragments arranged by size:
1876 1334 1038 879 524 492 305 298
BsaI GGTCTCn'nnnn_
Cuts at: 1384 2497 3224 6450 1384
Size: 1113 727 3226 1680
Fragments arranged by size:
3226 1680 1113 727
BsaAI yAC'GTr
Cuts at: 3012 3738 4099 3012
Size: 726 361 5659
Fragments arranged by size:
5659 726 361
BsaBI GATnn'nnATC
Cuts at: 6 787 2578 4260 6297 6
Size: 781 1791 1682 2037 455
Fragments arranged by size:
2037 1791 1682 781 455
BsaHI Gr'CG_yC
Cuts at: 425 4177 4425 5127 6623 425
Size: 3752 248 702 1496 548
Fragments arranged by size:
3752 1496 702 548 248
BsaWI w'CCGG_w
Cuts at: 986 2303 3318 3935 4456 5134 5408 5555 5966
Size: 1317 1015 617 521 678 274 147 411
Cuts at: 5966 986
Size: 1766
Fragments arranged by size:
1766 1317 1015 678 617 521 411 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 433 463 1117 1147 2494 2524 433
Size: 30 654 30 1347 30 4655
Fragments arranged by size:
4655 1347 654 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 454 1102 2685 2872 3482 454
Size: 648 1583 187 610 3718
Fragments arranged by size:
3718 1583 648 610 187
BseYI C'CCAG_C
Cuts at: 334 4171 4929 5506 6002 334
Size: 3837 758 577 496 1078
Fragments arranged by size:
3837 1078 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 3132 3132
Size: 6746
BsiEI CG_ry'CG
Cuts at: 104 4288 4334 5542 104
Size: 4184 46 1208 1308
Fragments arranged by size:
4184 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 3412 3728 3760 4538 4728 5520 3412
Size: 316 32 778 190 792 4638
Fragments arranged by size:
4638 792 778 316 190 32
BsmI GAATG_Cn'
Cuts at: 921 1662 921
Size: 741 6005
Bsp1286I G_dGCh'C
Cuts at: 242 1005 1420 2189 2890 3145 3412 3554 3728
Size: 763 415 769 701 255 267 142 174
Cuts at: 3728 3760 4371 4464 4538 4728 4790 5520 242
Size: 32 611 93 74 190 62 730 1468
Fragments arranged by size:
1468 769 763 730 701 611 415 267
255 190 174 142 93 74 62 32
BspEI T'CCGG_A
Cuts at: 2303 3318 5966 2303
Size: 1015 2648 3083
Fragments arranged by size:
3083 2648 1015
BspHI T'CATG_A
Cuts at: 2570 2814 5922 2570
Size: 244 3108 3394
Fragments arranged by size:
3394 3108 244
BspMI ACCTGCnnnn'nnnn_
Cuts at: 831 3796 4010 4312 4693 831
Size: 2965 214 302 381 2884
Fragments arranged by size:
2965 2884 381 302 214
BsrBI CCG'CTC
Cuts at: 50 5038 5092 6576 6741 50
Size: 4988 54 1484 165 55
Fragments arranged by size:
4988 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 4658 4658
Size: 6746
BsrFI r'CCGG_y
Cuts at: 2587 2844 3240 4744 4925 5134 2587
Size: 257 396 1504 181 209 4199
Fragments arranged by size:
4199 1504 396 257 209 181
BsrGI T'GTAC_A
Cuts at: 651 651
Size: 6746
BssSI C'ACGA_G
Cuts at: 5017 5375 5017
Size: 358 6388
BstAPI GCAn_nnn'nTGC
Cuts at: 3703 4123 3703
Size: 420 6326
BstBI TT'CG_AA
Cuts at: 110 5107 110
Size: 4997 1749
BstEII G'GTnAC_C
Cuts at: 1155 1155
Size: 6746
BstYI r'GATC_y
Cuts at: 1 261 627 1254 1986 3771 4238 4261 4596
Size: 260 366 627 732 1785 467 23 335
Cuts at: 4596 4842 5854 5940 5952 6298 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1785 1012 732 627 467 449 366 346
335 260 246 86 23 12
BstZ17I GTA'TAC
Cuts at: 2270 2270
Size: 6746
Bsu36I CC'TnA_GG
Cuts at: 133 1204 3719 133
Size: 1071 2515 3160
Fragments arranged by size:
3160 2515 1071
BtgI C'CryG_G
Cuts at: 125 2210 2594 125
Size: 2085 384 4277
Fragments arranged by size:
4277 2085 384
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2568 4696 4877 5040 2568
Size: 2128 181 163 4274
Fragments arranged by size:
4274 2128 181 163
BtsI GCAGTG_nn'
Cuts at: 2237 2453 3219 3989 2237
Size: 216 766 770 4994
Fragments arranged by size:
4994 770 766 216
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1557 1592 1557
Size: 35 6711
DraI TTT'AAA
Cuts at: 882 1040 1437 1472 3745 882
Size: 158 397 35 2273 3883
Fragments arranged by size:
3883 2273 397 158 35
DraIII CAC_nnn'GTG
Cuts at: 1107 3012 1107
Size: 1905 4841
DrdI GACnn_nn'nnGTC
Cuts at: 4452 5310 4452
Size: 858 5888
EaeI y'GGCC_r
Cuts at: 727 830 2930 4331 4505 4896 4923 6268 727
Size: 103 2100 1401 174 391 27 1345 1205
Fragments arranged by size:
2100 1401 1345 1205 391 174 103 27
EagI C'GGCC_G
Cuts at: 4331 4331
Size: 6746
EarI CTCTTCn'nnn_
Cuts at: 1401 2783 3081 3278 4769 4979 1401
Size: 1382 298 197 1491 210 3168
Fragments arranged by size:
3168 1491 1382 298 210 197
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4954 5264 5410 6556 4954
Size: 310 146 1146 5144
Fragments arranged by size:
5144 1146 310 146
EcoICRI GAG'CTC
Cuts at: 3758 3758
Size: 6746
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 477 695 1689 1949 2018 2875 2972 3081 3353
Size: 218 994 260 69 857 97 109 272
Cuts at: 3353 3413 3700 4571 5003 5750 6018 477
Size: 60 287 871 432 747 268 1205
Fragments arranged by size:
1205 994 871 857 747 432 287 272
268 260 218 109 97 69 60
EcoNI CCTnn'n_nnAGG
Cuts at: 171 635 171
Size: 464 6282
EcoO109I rG'GnC_Cy
Cuts at: 1073 1344 1769 2886 3141 3142 3376 3885 1073
Size: 271 425 1117 255 1 234 509 3934
Fragments arranged by size:
3934 1117 509 425 271 255 234 1
EcoRI G'AATT_C
Cuts at: 1426 3298 3750 1426
Size: 1872 452 4422
Fragments arranged by size:
4422 1872 452
EcoRV GAT'ATC
Cuts at: 699 699
Size: 6746
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3162 4765 3162
Size: 1603 5143
FspI TGC'GCA
Cuts at: 4527 4527
Size: 6746
HaeII r_GCGC'y
Cuts at: 305 428 3171 4180 4428 5450 6429 305
Size: 123 2743 1009 248 1022 979 622
Fragments arranged by size:
2743 1022 1009 979 622 248 123
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 683 715 1117 1149 3298 3330 3331 3343 3363
Size: 32 402 32 2149 32 1 12 20
Cuts at: 3363 3375 3396 3428 4588 4618 4620 4650 683
Size: 12 21 32 1160 30 2 30 2779
Fragments arranged by size:
2779 2149 1160 402 32 32 32 32
30 30 21 20 12 12 2 1
HincII GTy'rAC
Cuts at: 2241 2385 2448 3785 2241
Size: 144 63 1337 5202
Fragments arranged by size:
5202 1337 144 63
HindIII A'AGCT_T
Cuts at: 1498 3538 1498
Size: 2040 4706
HpaI GTT'AAC
Cuts at: 2448 2448
Size: 6746
KasI G'GCGC_C
Cuts at: 424 4176 4424 424
Size: 3752 248 2746
Fragments arranged by size:
3752 2746 248
KpnI G_GTAC'C
Cuts at: 932 2406 3766 932
Size: 1474 1360 3912
Fragments arranged by size:
3912 1474 1360
MluI A'CGCG_T
Cuts at: 6179 6179
Size: 6746
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 416 1385 1964 2492 2923 5417 5601 416
Size: 969 579 528 431 2494 184 1561
Fragments arranged by size:
2494 1561 969 579 528 431 184
MscI TGG'CCA
Cuts at: 729 2932 4507 729
Size: 2203 1575 2968
Fragments arranged by size:
2968 2203 1575
MslI CAynn'nnrTG
Cuts at: 281 1306 2248 2425 2608 3098 4735 4862 281
Size: 1025 942 177 183 490 1637 127 2165
Fragments arranged by size:
2165 1637 1025 942 490 183 177 127
MspA1I CmG'CkG
Cuts at: 3857 4171 4531 5544 5789 3857
Size: 314 360 1013 245 4814
Fragments arranged by size:
4814 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2846 4927 2846
Size: 2081 4665
NarI GG'CG_CC
Cuts at: 425 4177 4425 425
Size: 3752 248 2746
Fragments arranged by size:
3752 2746 248
NcoI C'CATG_G
Cuts at: 125 2594 125
Size: 2469 4277
NdeI CA'TA_TG
Cuts at: 432 432
Size: 6746
NgoMIV G'CCGG_C
Cuts at: 2844 4925 2844
Size: 2081 4665
NsiI A_TGCA'T
Cuts at: 2649 2649
Size: 6746
NspI r_CATG'y
Cuts at: 3799 4299 5206 3799
Size: 500 907 5339
Fragments arranged by size:
5339 907 500
PciI A'CATG_T
Cuts at: 5202 5202
Size: 6746
PflMI CCAn_nnn'nTGG
Cuts at: 3569 3569
Size: 6746
PmeI GTTT'AAAC
Cuts at: 3745 3745
Size: 6746
PmlI CAC'GTG
Cuts at: 3012 3012
Size: 6746
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5885 5917 5885
Size: 32 6714
PpuMI rG'GwC_Cy
Cuts at: 1073 1344 1769 3376 1073
Size: 271 425 1607 4443
Fragments arranged by size:
4443 1607 425 271
PshAI GACnn'nnGTC
Cuts at: 3221 3221
Size: 6746
PsiI TTA'TAA
Cuts at: 1216 3689 1216
Size: 2473 4273
PspOMI G'GGCC_C
Cuts at: 2886 3141 2886
Size: 255 6491
PspXI vC'TCGA_Gb
Cuts at: 1145 1145
Size: 6746
PstI C_TGCA'G
Cuts at: 842 3214 3793 4478 842
Size: 2372 579 685 3110
Fragments arranged by size:
3110 2372 685 579
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6746
PvuII CAG'CTG
Cuts at: 4171 4531 4171
Size: 360 6386
RsrII CG'GwC_CG
Cuts at: 4941 4941
Size: 6746
SacI G_AGCT'C
Cuts at: 3760 3760
Size: 6746
SalI G'TCGA_C
Cuts at: 3783 3783
Size: 6746
SanDI GG'GwC_CC
Cuts at: 1769 3376 1769
Size: 1607 5139
SapI GCTCTTCn'nnn_
Cuts at: 3081 4769 4979 3081
Size: 1688 210 4848
Fragments arranged by size:
4848 1688 210
SbfI CC_TGCA'GG
Cuts at: 3793 3793
Size: 6746
ScaI AGT'ACT
Cuts at: 1893 6263 1893
Size: 4370 2376
SfcI C'TryA_G
Cuts at: 33 315 838 2273 3210 3398 3789 4474 5467
Size: 282 523 1435 937 188 391 685 993
Cuts at: 5467 5658 33
Size: 191 1121
Fragments arranged by size:
1435 1121 993 937 685 523 391 282
191 188
SfoI GGC'GCC
Cuts at: 426 4178 4426 426
Size: 3752 248 2746
Fragments arranged by size:
3752 2746 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6746
SgrAI Cr'CCGG_yG
Cuts at: 2587 2587
Size: 6746
SmaI CCC'GGG
Cuts at: 3768 3768
Size: 6746
SmlI C'TyrA_G
Cuts at: 194 463 1145 1501 3413 3901 4816 5308 5570
Size: 269 682 356 1912 488 915 492 262
Cuts at: 5570 194
Size: 1370
Fragments arranged by size:
1912 1370 915 682 492 488 356 269
262
SnaBI TAC'GTA
Cuts at: 3738 3738
Size: 6746
SpeI A'CTAG_T
Cuts at: 67 3082 67
Size: 3015 3731
SphI G_CATG'C
Cuts at: 3799 4299 3799
Size: 500 6246
SspI AAT'ATT
Cuts at: 570 1381 570
Size: 811 5935
StuI AGG'CCT
Cuts at: 3313 3313
Size: 6746
StyI C'CwwG_G
Cuts at: 125 2594 3120 3880 4234 125
Size: 2469 526 760 354 2637
Fragments arranged by size:
2637 2469 760 526 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1668 1694 3513 4329 4462 4921 5130 1668
Size: 26 1819 816 133 459 209 3284
Fragments arranged by size:
3284 1819 816 459 209 133 26
TatI w'GTAC_w
Cuts at: 651 1891 6261 651
Size: 1240 4370 1136
Fragments arranged by size:
4370 1240 1136
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 433 465 6263 6295 433
Size: 32 5798 32 884
Fragments arranged by size:
5798 884 32 32
Tth111I GACn'n_nGTC
Cuts at: 4543 4543
Size: 6746
XbaI T'CTAG_A
Cuts at: 3777 3777
Size: 6746
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3640 4241 3640
Size: 601 6145
XhoI C'TCGA_G
Cuts at: 1145 1145
Size: 6746
XmaI C'CCGG_G
Cuts at: 3766 3766
Size: 6746
XmnI GAAnn'nnTTC
Cuts at: 3595 3595
Size: 6746
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BfrBI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NsiI NspI PciI PflMI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PstI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI AscI
AvrII BcgI BglI BmtI BsiWI BsmBI BssHII BstXI
ClaI FseI FspAI MfeI NheI NotI NruI PacI
PasI PfoI PsrI SacII SexAI SfiI SrfI SwaI
ZraI