Restriction map for
pF1KSDB0238
(Circular) (Six-base) MAPSORT of: pF1KSDB0238.seq Check: 4985 from: 1 to: 6908
pF1KSDB0238 6908 bp
With 182 enzymes: SgfI *
June 20, 2008 10:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2006 3092 2006
Size: 1086 5822
AccI GT'mk_AC
Cuts at: 3946 3946
Size: 6908
Acc65I G'GTAC_C
Cuts at: 3089 3924 3089
Size: 835 6073
AclI AA'CG_TT
Cuts at: 6748 6748
Size: 6908
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1516 1681 2841 3088 3243 4733 5165 5912 6180
Size: 165 1160 247 155 1490 432 747 268
Cuts at: 6180 1516
Size: 2244
Fragments arranged by size:
2244 1490 1160 747 432 268 247 165
155
AflIII A'CryG_T
Cuts at: 146 2456 4260 5364 6341 146
Size: 2310 1804 1104 977 713
Fragments arranged by size:
2310 1804 1104 977 713
AgeI A'CCGG_T
Cuts at: 5296 5296
Size: 6908
AleI CACnn'nnGTG
Cuts at: 1324 2467 3604 1324
Size: 1143 1137 4628
Fragments arranged by size:
4628 1143 1137
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 670 670
Size: 6908
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1532 1564 3394 3426 3763 3781 3795 3813 1532
Size: 32 1830 32 337 18 14 18 4627
Fragments arranged by size:
4627 1830 337 32 32 18 18 14
AlwNI CAG_nnn'CTG
Cuts at: 173 1023 1506 4213 5780 173
Size: 850 483 2707 1567 1301
Fragments arranged by size:
2707 1567 1301 850 483
ApaI G_GGCC'C
Cuts at: 1697 2411 2840 3127 1697
Size: 714 429 287 5478
Fragments arranged by size:
5478 714 429 287
ApaLI G'TGCA_C
Cuts at: 5678 5678
Size: 6908
ApoI r'AATT_y
Cuts at: 2077 3912 6261 6517 2077
Size: 1835 2349 256 2468
Fragments arranged by size:
2468 2349 1835 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6908
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6908
AvaI C'yCGr_G
Cuts at: 193 2215 2887 3928 193
Size: 2022 672 1041 3173
Fragments arranged by size:
3173 2022 1041 672
BamHI G'GATC_C
Cuts at: 1235 3933 1235
Size: 2698 4210
BanI G'GyrC_C
Cuts at: 254 487 526 769 1285 1847 1968 2097 2387
Size: 233 39 243 516 562 121 129 290
Cuts at: 2387 2742 3089 3416 3924 4338 4586 4621 254
Size: 355 347 327 508 414 248 35 2541
Fragments arranged by size:
2541 562 516 508 414 355 347 327
290 248 243 233 129 121 39 35
BanII G_rGCy'C
Cuts at: 1361 1697 1960 2225 2411 2525 2840 3127 3346
Size: 336 263 265 186 114 315 287 219
Cuts at: 3346 3922 4952 1361
Size: 576 1030 3317
Fragments arranged by size:
3317 1030 576 336 315 287 265 263
219 186 114
BbeI G_GCGC'C
Cuts at: 258 1289 1851 2101 2391 4342 4590 258
Size: 1031 562 250 290 1951 248 2576
Fragments arranged by size:
2576 1951 1031 562 290 250 248
BbsI GAAGACnn'nnnn_
Cuts at: 258 2048 3439 258
Size: 1790 1391 3727
Fragments arranged by size:
3727 1790 1391
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1458 1492 1689 1723 1458
Size: 34 197 34 6643
Fragments arranged by size:
6643 197 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2659 4806 5567 6908 2659
Size: 2147 761 1341 2659
Fragments arranged by size:
2659 2147 1341 761
BclI T'GATC_A
Cuts at: 4428 4428
Size: 6908
BglI GCCn_nnn'nGGC
Cuts at: 695 965 2960 695
Size: 270 1995 4643
Fragments arranged by size:
4643 1995 270
BglII A'GATC_T
Cuts at: 650 4423 6460 650
Size: 3773 2037 1098
Fragments arranged by size:
3773 2037 1098
BlpI GC'TnA_GC
Cuts at: 4020 4020
Size: 6908
Bme1580I G_kGCm'C
Cuts at: 490 531 1089 1493 1697 1938 2411 2474 2747
Size: 41 558 404 204 241 473 63 273
Cuts at: 2747 2840 3127 4533 4626 5682 490
Size: 93 287 1406 93 1056 1716
Fragments arranged by size:
1716 1406 1056 558 473 404 287 273
241 204 93 93 63 41
BmgBI CAC'GTC
Cuts at: 2459 2459
Size: 6908
BmrI ACTGGGnnnn_n'
Cuts at: 956 1087 1307 1487 4120 4201 4536 6680 956
Size: 131 220 180 2633 81 335 2144 1184
Fragments arranged by size:
2633 2144 1184 335 220 180 131 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3479 5275 3479
Size: 1796 5112
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 852 931 1242 1570 1789 2049 2567 2703 2824
Size: 79 311 328 219 260 518 136 121
Cuts at: 2824 2923 3682 852
Size: 99 759 4078
Fragments arranged by size:
4078 759 518 328 311 260 219 136
121 99 79
Bpu10I CC'TnA_GC
Cuts at: 1297 2573 3855 1297
Size: 1276 1282 4350
Fragments arranged by size:
4350 1282 1276
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 274 2140 2586 4084 4963 5455 5753 274
Size: 1866 446 1498 879 492 298 1429
Fragments arranged by size:
1866 1498 1429 879 492 446 298
BsaI GGTCTCn'nnnn_
Cuts at: 1574 1793 2238 2299 3631 6612 1574
Size: 219 445 61 1332 2981 1870
Fragments arranged by size:
2981 1870 1332 445 219 61
BsaAI yAC'GTr
Cuts at: 147 1739 2327 2979 3900 4261 147
Size: 1592 588 652 921 361 2794
Fragments arranged by size:
2794 1592 921 652 588 361
BsaBI GATnn'nnATC
Cuts at: 6 4422 6459 6
Size: 4416 2037 455
Fragments arranged by size:
4416 2037 455
BsaHI Gr'CG_yC
Cuts at: 255 1286 1848 2098 2388 4339 4587 5289 6785
Size: 1031 562 250 290 1951 248 702 1496
Cuts at: 6785 255
Size: 378
Fragments arranged by size:
1951 1496 1031 702 562 378 290 250
248
BsaWI w'CCGG_w
Cuts at: 626 1271 1600 2339 4097 4618 5296 5570 5717
Size: 645 329 739 1758 521 678 274 147
Cuts at: 5717 6128 626
Size: 411 1406
Fragments arranged by size:
1758 1406 739 678 645 521 411 329
274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 388 418 1472 1502 2896 2926 2968 2998 3235
Size: 30 1054 30 1394 30 42 30 237
Cuts at: 3235 3265 3328 3358 3394 3424 388
Size: 30 63 30 36 30 3872
Fragments arranged by size:
3872 1394 1054 237 63 42 36 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 148 151 1051 1261 3517 3691 148
Size: 3 900 210 2256 174 3365
Fragments arranged by size:
3365 2256 900 210 174 3
BseYI C'CCAG_C
Cuts at: 369 483 1771 2224 4333 5091 5668 6164 369
Size: 114 1288 453 2109 758 577 496 1113
Fragments arranged by size:
2109 1288 1113 758 577 496 453 114
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 340 635 1117 1327 1978 2401 2830 340
Size: 295 482 210 651 423 429 4418
Fragments arranged by size:
4418 651 482 429 423 295 210
BsiEI CG_ry'CG
Cuts at: 104 984 1797 2629 3625 4450 4496 5704 104
Size: 880 813 832 996 825 46 1208 1308
Fragments arranged by size:
1308 1208 996 880 832 825 813 46
BsiHKAI G_wGCw'C
Cuts at: 722 2969 2984 3346 3922 4700 4890 5682 722
Size: 2247 15 362 576 778 190 792 1948
Fragments arranged by size:
2247 1948 792 778 576 362 190 15
BsiWI C'GTAC_G
Cuts at: 3141 3141
Size: 6908
BsmI GAATG_Cn'
Cuts at: 1574 1574
Size: 6908
BsmBI CGTCTCn'nnnn_
Cuts at: 3231 3231
Size: 6908
Bsp1286I G_dGCh'C
Cuts at: 490 531 722 1089 1361 1493 1697 1938 1960
Size: 41 191 367 272 132 204 241 22
Cuts at: 1960 2225 2411 2474 2525 2747 2840 2969 2984
Size: 265 186 63 51 222 93 129 15
Cuts at: 2984 3127 3346 3922 4533 4626 4700 4890 4952
Size: 143 219 576 611 93 74 190 62
Cuts at: 4952 5682 490
Size: 730 1716
Fragments arranged by size:
1716 730 611 576 367 272 265 241
222 219 204 191 190 186 143 132
129 93 93 74 63 62 51 41
22 15
BspEI T'CCGG_A
Cuts at: 626 6128 626
Size: 5502 1406
BspHI T'CATG_A
Cuts at: 6084 6084
Size: 6908
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2000 2006 3092 3371 3958 4172 4474 4855 2000
Size: 6 1086 279 587 214 302 381 4053
Fragments arranged by size:
4053 1086 587 381 302 279 214 6
BsrBI CCG'CTC
Cuts at: 50 675 2337 5200 5254 6738 6903 50
Size: 625 1662 2863 54 1484 165 55
Fragments arranged by size:
2863 1662 1484 625 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3852 4820 3852
Size: 968 5940
BsrFI r'CCGG_y
Cuts at: 1349 1965 2628 2971 4906 5087 5296 1349
Size: 616 663 343 1935 181 209 2961
Fragments arranged by size:
2961 1935 663 616 343 209 181
BssSI C'ACGA_G
Cuts at: 2402 5179 5537 2402
Size: 2777 358 3773
Fragments arranged by size:
3773 2777 358
BstAPI GCAn_nnn'nTGC
Cuts at: 173 4285 173
Size: 4112 2796
BstBI TT'CG_AA
Cuts at: 110 5269 110
Size: 5159 1749
BstEII G'GTnAC_C
Cuts at: 1215 1326 1728 1215
Size: 111 402 6395
Fragments arranged by size:
6395 402 111
BstXI CCAn_nnnn'nTGG
Cuts at: 132 1156 1813 2316 3814 132
Size: 1024 657 503 1498 3226
Fragments arranged by size:
3226 1498 1024 657 503
BstYI r'GATC_y
Cuts at: 1 188 506 629 650 1235 1406 3173 3386
Size: 187 318 123 21 585 171 1767 213
Cuts at: 3386 3933 4400 4423 4758 5004 6016 6102 6114
Size: 547 467 23 335 246 1012 86 12
Cuts at: 6114 6460 1
Size: 346 449
Fragments arranged by size:
1767 1012 585 547 467 449 346 335
318 246 213 187 171 123 86 23
21 12
Bsu36I CC'TnA_GG
Cuts at: 2255 2393 2255
Size: 138 6770
BtgI C'CryG_G
Cuts at: 125 250 560 1109 1972 2661 3884 125
Size: 125 310 549 863 689 1223 3149
Fragments arranged by size:
3149 1223 863 689 549 310 125
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 903 3689 4858 5039 5202 903
Size: 2786 1169 181 163 2609
Fragments arranged by size:
2786 2609 1169 181 163
BtsI GCAGTG_nn'
Cuts at: 1089 1451 2389 3361 3741 4151 1089
Size: 362 938 972 380 410 3846
Fragments arranged by size:
3846 972 938 410 380 362
DraI TTT'AAA
Cuts at: 3907 3907
Size: 6908
DraIII CAC_nnn'GTG
Cuts at: 216 882 3356 216
Size: 666 2474 3768
Fragments arranged by size:
3768 2474 666
DrdI GACnn_nn'nnGTC
Cuts at: 808 1189 4614 5472 808
Size: 381 3425 858 2244
Fragments arranged by size:
3425 2244 858 381
EaeI y'GGCC_r
Cuts at: 518 538 1064 1253 1367 1901 2626 2880 3749
Size: 20 526 189 114 534 725 254 869
Cuts at: 3749 3804 4493 4667 5058 5085 6430 518
Size: 55 689 174 391 27 1345 996
Fragments arranged by size:
1345 996 869 725 689 534 526 391
254 189 174 114 55 27 20
EagI C'GGCC_G
Cuts at: 2626 4493 2626
Size: 1867 5041
EarI CTCTTCn'nnn_
Cuts at: 359 859 3287 4931 5141 359
Size: 500 2428 1644 210 2126
Fragments arranged by size:
2428 2126 1644 500 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 5116 5426 5572 6718 5116
Size: 310 146 1146 5306
Fragments arranged by size:
5306 1146 310 146
EcoICRI GAG'CTC
Cuts at: 3344 3920 3344
Size: 576 6332
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 852 931 1242 1516 1570 1681 1789 2049 2567
Size: 79 311 274 54 111 108 260 518
Cuts at: 2567 2703 2824 2841 2923 3088 3243 3682 4733
Size: 136 121 17 82 165 155 439 1051
Cuts at: 4733 5165 5912 6180 852
Size: 432 747 268 1580
Fragments arranged by size:
1580 1051 747 518 439 432 311 274
268 260 165 155 136 121 111 108
82 79 54 17
EcoNI CCTnn'n_nnAGG
Cuts at: 2641 3001 2641
Size: 360 6548
EcoO109I rG'GnC_Cy
Cuts at: 1202 1565 1693 1694 2407 2408 2836 3039 3123
Size: 363 128 1 713 1 428 203 84
Cuts at: 3123 3124 3234 3360 3656 4047 1202
Size: 1 110 126 296 391 4063
Fragments arranged by size:
4063 713 428 391 363 296 203 128
126 110 84 1 1 1
EcoRI G'AATT_C
Cuts at: 3912 3912
Size: 6908
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4927 4927
Size: 6908
FspI TGC'GCA
Cuts at: 4689 4689
Size: 6908
HaeII r_GCGC'y
Cuts at: 139 258 1289 1486 1851 2101 2391 4342 4590
Size: 119 1031 197 365 250 290 1951 248
Cuts at: 4590 5612 6591 139
Size: 1022 979 456
Fragments arranged by size:
1951 1031 1022 979 456 365 290 250
248 197 119
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 721 753 2896 2928 3640 3672 4750 4780 4782
Size: 32 2143 32 712 32 1078 30 2
Cuts at: 4782 4812 721
Size: 30 2817
Fragments arranged by size:
2817 2143 1078 712 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 2499 3947 2499
Size: 1448 5460
KasI G'GCGC_C
Cuts at: 254 1285 1847 2097 2387 4338 4586 254
Size: 1031 562 250 290 1951 248 2576
Fragments arranged by size:
2576 1951 1031 562 290 250 248
KpnI G_GTAC'C
Cuts at: 3093 3928 3093
Size: 835 6073
MluI A'CGCG_T
Cuts at: 6341 6341
Size: 6908
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 752 2012 2477 5579 5763 752
Size: 1260 465 3102 184 1897
Fragments arranged by size:
3102 1897 1260 465 184
MscI TGG'CCA
Cuts at: 540 1255 1369 1903 2882 3806 4669 540
Size: 715 114 534 979 924 863 2779
Fragments arranged by size:
2779 979 924 863 715 534 114
MslI CAynn'nnrTG
Cuts at: 130 493 1154 1324 2314 2467 3604 4897 5024
Size: 363 661 170 990 153 1137 1293 127
Cuts at: 5024 130
Size: 2014
Fragments arranged by size:
2014 1293 1137 990 661 363 170 153
127
MspA1I CmG'CkG
Cuts at: 329 464 1020 1058 1395 1895 2663 3205 4019
Size: 135 556 38 337 500 768 542 814
Cuts at: 4019 4333 4693 5706 5951 329
Size: 314 360 1013 245 1286
Fragments arranged by size:
1286 1013 814 768 556 542 500 360
337 314 245 135 38
NaeI GCC'GGC
Cuts at: 5089 5089
Size: 6908
NarI GG'CG_CC
Cuts at: 255 1286 1848 2098 2388 4339 4587 255
Size: 1031 562 250 290 1951 248 2576
Fragments arranged by size:
2576 1951 1031 562 290 250 248
NcoI C'CATG_G
Cuts at: 125 125
Size: 6908
NgoMIV G'CCGG_C
Cuts at: 5087 5087
Size: 6908
NspI r_CATG'y
Cuts at: 1096 3961 4461 5368 1096
Size: 2865 500 907 2636
Fragments arranged by size:
2865 2636 907 500
PasI CC'CwG_GG
Cuts at: 1569 1960 3729 1569
Size: 391 1769 4748
Fragments arranged by size:
4748 1769 391
PciI A'CATG_T
Cuts at: 5364 5364
Size: 6908
PflMI CCAn_nnn'nTGG
Cuts at: 219 882 950 1866 2739 2997 219
Size: 663 68 916 873 258 4130
Fragments arranged by size:
4130 916 873 663 258 68
PfoI T'CCnGG_A
Cuts at: 908 1805 2026 908
Size: 897 221 5790
Fragments arranged by size:
5790 897 221
PmeI GTTT'AAAC
Cuts at: 3907 3907
Size: 6908
PmlI CAC'GTG
Cuts at: 1739 2327 1739
Size: 588 6320
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2128 2160 3394 3426 3475 3507 6047 6079 2128
Size: 32 1234 32 49 32 2540 32 2957
Fragments arranged by size:
2957 2540 1234 49 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 1565 3039 3234 3360 3656 1565
Size: 1474 195 126 296 4817
Fragments arranged by size:
4817 1474 296 195 126
PshAI GACnn'nnGTC
Cuts at: 2245 2245
Size: 6908
PspOMI G'GGCC_C
Cuts at: 1693 2407 2836 3123 1693
Size: 714 429 287 5478
Fragments arranged by size:
5478 714 429 287
PstI C_TGCA'G
Cuts at: 169 1019 2040 3368 3955 4640 169
Size: 850 1021 1328 587 685 2437
Fragments arranged by size:
2437 1328 1021 850 685 587
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6908
PvuII CAG'CTG
Cuts at: 1020 1395 1895 4333 4693 1020
Size: 375 500 2438 360 3235
Fragments arranged by size:
3235 2438 500 375 360
RsrII CG'GwC_CG
Cuts at: 5103 5103
Size: 6908
SacI G_AGCT'C
Cuts at: 3346 3922 3346
Size: 576 6332
SacII CC_GC'GG
Cuts at: 2664 2664
Size: 6908
SalI G'TCGA_C
Cuts at: 3945 3945
Size: 6908
SanDI GG'GwC_CC
Cuts at: 1565 3039 3234 1565
Size: 1474 195 5239
Fragments arranged by size:
5239 1474 195
SapI GCTCTTCn'nnn_
Cuts at: 359 859 4931 5141 359
Size: 500 4072 210 2126
Fragments arranged by size:
4072 2126 500 210
SbfI CC_TGCA'GG
Cuts at: 3955 3955
Size: 6908
ScaI AGT'ACT
Cuts at: 1831 6425 1831
Size: 4594 2314
SexAI A'CCwGG_T
Cuts at: 878 878
Size: 6908
SfcI C'TryA_G
Cuts at: 33 165 1015 1872 1890 2036 2787 3216 3364
Size: 132 850 857 18 146 751 429 148
Cuts at: 3364 3951 4636 5629 5820 33
Size: 587 685 993 191 1121
Fragments arranged by size:
1121 993 857 850 751 685 587 429
191 148 146 132 18
SfoI GGC'GCC
Cuts at: 256 1287 1849 2099 2389 4340 4588 256
Size: 1031 562 250 290 1951 248 2576
Fragments arranged by size:
2576 1951 1031 562 290 250 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6908
SmaI CCC'GGG
Cuts at: 2217 3930 2217
Size: 1713 5195
SmlI C'TyrA_G
Cuts at: 289 2155 2601 4063 4978 5470 5732 289
Size: 1866 446 1462 915 492 262 1465
Fragments arranged by size:
1866 1465 1462 915 492 446 262
SnaBI TAC'GTA
Cuts at: 3900 3900
Size: 6908
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6908
SphI G_CATG'C
Cuts at: 1096 3961 4461 1096
Size: 2865 500 3543
Fragments arranged by size:
3543 2865 500
StuI AGG'CCT
Cuts at: 1474 1747 2815 3685 1474
Size: 273 1068 870 4697
Fragments arranged by size:
4697 1068 870 273
StyI C'CwwG_G
Cuts at: 125 359 3239 3284 4042 4396 125
Size: 234 2880 45 758 354 2637
Fragments arranged by size:
2880 2637 758 354 234 45
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 361 3082 3616 3639 4491 4624 5083 5292 361
Size: 2721 534 23 852 133 459 209 1977
Fragments arranged by size:
2721 1977 852 534 459 209 133 23
TatI w'GTAC_w
Cuts at: 1829 3650 3809 6423 1829
Size: 1821 159 2614 2314
Fragments arranged by size:
2614 2314 1821 159
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 200 232 388 420 1266 1298 1472 1504 2657
Size: 32 156 32 846 32 174 32 1153
Cuts at: 2657 2689 3235 3267 6425 6457 200
Size: 32 546 32 3158 32 651
Fragments arranged by size:
3158 1153 846 651 546 174 156 32
32 32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 985 4705 985
Size: 3720 3188
XbaI T'CTAG_A
Cuts at: 3939 3939
Size: 6908
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 366 480 876 1217 1320 1566 1911 4403 366
Size: 114 396 341 103 246 345 2492 2871
Fragments arranged by size:
2871 2492 396 345 341 246 114 103
XmaI C'CCGG_G
Cuts at: 2215 3928 2215
Size: 1713 5195
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PspOMI PstI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SnaBI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AscI AvrII BaeI BbvCI
BfrBI BmtI BsrGI BssHII BstZ17I ClaI CspCI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PsiI PspXI PsrI SfiI SgrAI
SrfI SspI SwaI XhoI XmnI ZraI