Restriction map for
pF1KSDB1316
(Circular) (Six-base) MAPSORT of: pF1KSDB1316.seq Check: 6369 from: 1 to: 5723
pF1KSDB1316 5723 bp
With 182 enzymes: SgfI *
June 20, 2008 10:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1657 1657
Size: 5723
AatII G_ACGT'C
Cuts at: 658 2669 658
Size: 2011 3712
AccI GT'mk_AC
Cuts at: 818 2761 818
Size: 1943 3780
Acc65I G'GTAC_C
Cuts at: 1209 2739 1209
Size: 1530 4193
AclI AA'CG_TT
Cuts at: 5563 5563
Size: 5723
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1057 2512 2701 3548 3980 4727 4995 1057
Size: 1455 189 847 432 747 268 1785
Fragments arranged by size:
1785 1455 847 747 432 268 189
AflII C'TTAA_G
Cuts at: 2308 2308
Size: 5723
AflIII A'CryG_T
Cuts at: 497 3075 4179 5156 497
Size: 2578 1104 977 1064
Fragments arranged by size:
2578 1104 1064 977
AgeI A'CCGG_T
Cuts at: 4111 4111
Size: 5723
AleI CACnn'nnGTG
Cuts at: 835 1165 2137 835
Size: 330 972 4421
Fragments arranged by size:
4421 972 330
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 909 909
Size: 5723
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 532 564 532
Size: 32 5691
AlwNI CAG_nnn'CTG
Cuts at: 1416 1551 1998 2490 3028 4595 1416
Size: 135 447 492 538 1567 2544
Fragments arranged by size:
2544 1567 538 492 447 135
ApaLI G'TGCA_C
Cuts at: 135 235 1101 4493 135
Size: 100 866 3392 1365
Fragments arranged by size:
3392 1365 866 100
ApoI r'AATT_y
Cuts at: 696 1278 2422 2727 5076 5332 696
Size: 582 1144 305 2349 256 1087
Fragments arranged by size:
2349 1144 1087 582 305 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5723
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5723
AvaI C'yCGr_G
Cuts at: 323 1029 1486 1602 1874 2743 323
Size: 706 457 116 272 869 3303
Fragments arranged by size:
3303 869 706 457 272 116
BamHI G'GATC_C
Cuts at: 2748 2748
Size: 5723
BanI G'GyrC_C
Cuts at: 319 1041 1209 1645 2739 3153 3401 3436 319
Size: 722 168 436 1094 414 248 35 2606
Fragments arranged by size:
2606 1094 722 436 414 248 168 35
BanII G_rGCy'C
Cuts at: 1467 1696 1738 1921 2737 3767 1467
Size: 229 42 183 816 1030 3423
Fragments arranged by size:
3423 1030 816 229 183 42
BbeI G_GCGC'C
Cuts at: 3157 3405 3157
Size: 248 5475
BbsI GAAGACnn'nnnn_
Cuts at: 327 404 984 1005 1125 1553 2022 2156 327
Size: 77 580 21 120 428 469 134 3894
Fragments arranged by size:
3894 580 469 428 134 120 77 21
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 947 981 2626 2660 947
Size: 34 1645 34 4010
Fragments arranged by size:
4010 1645 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2392 3621 4382 5723 2392
Size: 1229 761 1341 2392
Fragments arranged by size:
2392 1341 1229 761
BclI T'GATC_A
Cuts at: 3243 3243
Size: 5723
BglI GCCn_nnn'nGGC
Cuts at: 328 800 1624 2188 328
Size: 472 824 564 3863
Fragments arranged by size:
3863 824 564 472
BglII A'GATC_T
Cuts at: 3238 5275 3238
Size: 2037 3686
BlpI GC'TnA_GC
Cuts at: 289 2835 289
Size: 2546 3177
Bme1580I G_kGCm'C
Cuts at: 139 239 324 1105 1786 3348 3441 4497 139
Size: 100 85 781 681 1562 93 1056 1365
Fragments arranged by size:
1562 1365 1056 781 681 100 93 85
BmgBI CAC'GTC
Cuts at: 771 2052 771
Size: 1281 4442
BmrI ACTGGGnnnn_n'
Cuts at: 2935 3016 3351 5495 2935
Size: 81 335 2144 3163
Fragments arranged by size:
3163 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1152 4090 1152
Size: 2938 2785
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1247 1710 1247
Size: 463 5260
Bpu10I CC'TnA_GC
Cuts at: 1021 1021
Size: 5723
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2899 3778 4270 4568 2899
Size: 879 492 298 4054
Fragments arranged by size:
4054 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 396 1507 2566 5427 396
Size: 1111 1059 2861 692
Fragments arranged by size:
2861 1111 1059 692
BsaAI yAC'GTr
Cuts at: 498 2715 3076 498
Size: 2217 361 3145
Fragments arranged by size:
3145 2217 361
BsaBI GATnn'nnATC
Cuts at: 6 2263 3237 5274 6
Size: 2257 974 2037 455
Fragments arranged by size:
2257 2037 974 455
BsaHI Gr'CG_yC
Cuts at: 655 2666 3154 3402 4104 5600 655
Size: 2011 488 248 702 1496 778
Fragments arranged by size:
2011 1496 778 702 488 248
BsaWI w'CCGG_w
Cuts at: 1403 2912 3433 4111 4385 4532 4943 1403
Size: 1509 521 678 274 147 411 2183
Fragments arranged by size:
2183 1509 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 838 868 1255 1285 2202 2232 2699 2729 838
Size: 30 387 30 917 30 467 30 3832
Fragments arranged by size:
3832 917 467 387 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1141 1525 2020 2187 2320 1141
Size: 384 495 167 133 4544
Fragments arranged by size:
4544 495 384 167 133
BseYI C'CCAG_C
Cuts at: 603 1517 1738 2178 2279 2525 3148 3906 4483
Size: 914 221 440 101 246 623 758 577
Cuts at: 4483 4979 603
Size: 496 1347
Fragments arranged by size:
1347 914 758 623 577 496 440 246
221 101
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 265 437 1453 1705 2206 265
Size: 172 1016 252 501 3782
Fragments arranged by size:
3782 1016 501 252 172
BsiEI CG_ry'CG
Cuts at: 104 3265 3311 4519 104
Size: 3161 46 1208 1308
Fragments arranged by size:
3161 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 139 227 239 1022 1105 1162 1467 1493 2543
Size: 88 12 783 83 57 305 26 1050
Cuts at: 2543 2737 3515 3705 4497 139
Size: 194 778 190 792 1365
Fragments arranged by size:
1365 1050 792 783 778 305 194 190
88 83 57 26 12
BsmI GAATG_Cn'
Cuts at: 514 514
Size: 5723
BsmBI CGTCTCn'nnnn_
Cuts at: 2491 2491
Size: 5723
Bsp1286I G_dGCh'C
Cuts at: 139 227 239 324 1022 1105 1162 1467 1493
Size: 88 12 85 698 83 57 305 26
Cuts at: 1493 1696 1738 1786 1921 2543 2737 3348 3441
Size: 203 42 48 135 622 194 611 93
Cuts at: 3441 3515 3705 3767 4497 139
Size: 74 190 62 730 1365
Fragments arranged by size:
1365 730 698 622 611 305 203 194
190 135 93 88 85 83 74 62
57 48 42 26 12
BspEI T'CCGG_A
Cuts at: 4943 4943
Size: 5723
BspHI T'CATG_A
Cuts at: 252 4899 252
Size: 4647 1076
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1657 1717 2411 2773 2987 3289 3670 1657
Size: 60 694 362 214 302 381 3710
Fragments arranged by size:
3710 694 381 362 302 214 60
BsrBI CCG'CTC
Cuts at: 50 1766 2429 4015 4069 5553 5718 50
Size: 1716 663 1586 54 1484 165 55
Fragments arranged by size:
1716 1586 1484 663 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3635 3635
Size: 5723
BsrFI r'CCGG_y
Cuts at: 522 1808 2236 2563 3721 3902 4111 522
Size: 1286 428 327 1158 181 209 2134
Fragments arranged by size:
2134 1286 1158 428 327 209 181
BsrGI T'GTAC_A
Cuts at: 311 311
Size: 5723
BssSI C'ACGA_G
Cuts at: 3994 4352 3994
Size: 358 5365
BstAPI GCAn_nnn'nTGC
Cuts at: 243 415 2246 3100 243
Size: 172 1831 854 2866
Fragments arranged by size:
2866 1831 854 172
BstBI TT'CG_AA
Cuts at: 110 4084 110
Size: 3974 1749
BstEII G'GTnAC_C
Cuts at: 1335 1335
Size: 5723
BstXI CCAn_nnnn'nTGG
Cuts at: 2142 2533 2142
Size: 391 5332
BstYI r'GATC_y
Cuts at: 1 2748 3215 3238 3573 3819 4831 4917 4929
Size: 2747 467 23 335 246 1012 86 12
Cuts at: 4929 5275 1
Size: 346 449
Fragments arranged by size:
2747 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 916 916
Size: 5723
BtgI C'CryG_G
Cuts at: 877 1140 1219 2606 877
Size: 263 79 1387 3994
Fragments arranged by size:
3994 1387 263 79
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 202 1784 3673 3854 4017 202
Size: 1582 1889 181 163 1908
Fragments arranged by size:
1908 1889 1582 181 163
BtsI GCAGTG_nn'
Cuts at: 176 1103 2966 176
Size: 927 1863 2933
Fragments arranged by size:
2933 1863 927
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1812 1847 1812
Size: 35 5688
DraI TTT'AAA
Cuts at: 2722 2722
Size: 5723
DraIII CAC_nnn'GTG
Cuts at: 243 1187 243
Size: 944 4779
DrdI GACnn_nn'nnGTC
Cuts at: 3429 4287 3429
Size: 858 4865
EaeI y'GGCC_r
Cuts at: 973 3308 3482 3873 3900 5245 973
Size: 2335 174 391 27 1345 1451
Fragments arranged by size:
2335 1451 1345 391 174 27
EagI C'GGCC_G
Cuts at: 3308 3308
Size: 5723
EarI CTCTTCn'nnn_
Cuts at: 1066 1528 2347 2353 2368 3746 3956 1066
Size: 462 819 6 15 1378 210 2833
Fragments arranged by size:
2833 1378 819 462 210 15 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3931 4241 4387 5533 3931
Size: 310 146 1146 4121
Fragments arranged by size:
4121 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1465 2735 1465
Size: 1270 4453
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1057 1247 1710 2512 2701 3548 3980 4727 4995
Size: 190 463 802 189 847 432 747 268
Cuts at: 4995 1057
Size: 1785
Fragments arranged by size:
1785 847 802 747 463 432 268 190
189
EcoNI CCTnn'n_nnAGG
Cuts at: 2002 2002
Size: 5723
EcoO109I rG'GnC_Cy
Cuts at: 920 1025 1413 1617 1674 2862 920
Size: 105 388 204 57 1188 3781
Fragments arranged by size:
3781 1188 388 204 105 57
EcoRI G'AATT_C
Cuts at: 2727 2727
Size: 5723
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 324 3742 324
Size: 3418 2305
FspI TGC'GCA
Cuts at: 3504 3504
Size: 5723
HaeII r_GCGC'y
Cuts at: 485 3157 3405 4427 5406 485
Size: 2672 248 1022 979 802
Fragments arranged by size:
2672 1022 979 802 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1220 1252 3565 3595 3597 3627 1220
Size: 32 2313 30 2 30 3316
Fragments arranged by size:
3316 2313 32 30 30 2
HincII GTy'rAC
Cuts at: 2762 2762
Size: 5723
KasI G'GCGC_C
Cuts at: 3153 3401 3153
Size: 248 5475
KpnI G_GTAC'C
Cuts at: 1213 2743 1213
Size: 1530 4193
MluI A'CGCG_T
Cuts at: 5156 5156
Size: 5723
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1889 4394 4578 1889
Size: 2505 184 3034
Fragments arranged by size:
3034 2505 184
MscI TGG'CCA
Cuts at: 3484 3484
Size: 5723
MslI CAynn'nnrTG
Cuts at: 130 463 835 1165 2137 3712 3839 130
Size: 333 372 330 972 1575 127 2014
Fragments arranged by size:
2014 1575 972 372 333 330 127
MspA1I CmG'CkG
Cuts at: 983 1074 1221 1623 1840 2834 3148 3508 4521
Size: 91 147 402 217 994 314 360 1013
Cuts at: 4521 4766 983
Size: 245 1940
Fragments arranged by size:
1940 1013 994 402 360 314 245 217
147 91
NaeI GCC'GGC
Cuts at: 524 3904 524
Size: 3380 2343
NarI GG'CG_CC
Cuts at: 3154 3402 3154
Size: 248 5475
NgoMIV G'CCGG_C
Cuts at: 522 3902 522
Size: 3380 2343
NspI r_CATG'y
Cuts at: 1155 2776 3276 4183 1155
Size: 1621 500 907 2695
Fragments arranged by size:
2695 1621 907 500
PciI A'CATG_T
Cuts at: 4179 4179
Size: 5723
PflMI CCAn_nnn'nTGG
Cuts at: 1395 1416 1395
Size: 21 5702
PfoI T'CCnGG_A
Cuts at: 1526 2054 1526
Size: 528 5195
PmeI GTTT'AAAC
Cuts at: 2722 2722
Size: 5723
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 844 876 2668 2700 4862 4894 844
Size: 32 1792 32 2162 32 1673
Fragments arranged by size:
2162 1792 1673 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 920 1025 1674 920
Size: 105 649 4969
Fragments arranged by size:
4969 649 105
PspXI vC'TCGA_Gb
Cuts at: 1486 1486
Size: 5723
PstI C_TGCA'G
Cuts at: 473 1301 2770 3455 473
Size: 828 1469 685 2741
Fragments arranged by size:
2741 1469 828 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5723
PvuII CAG'CTG
Cuts at: 1074 3148 3508 1074
Size: 2074 360 3289
Fragments arranged by size:
3289 2074 360
RsrII CG'GwC_CG
Cuts at: 3918 3918
Size: 5723
SacI G_AGCT'C
Cuts at: 1467 2737 1467
Size: 1270 4453
SacII CC_GC'GG
Cuts at: 1222 1222
Size: 5723
SalI G'TCGA_C
Cuts at: 2760 2760
Size: 5723
SanDI GG'GwC_CC
Cuts at: 1674 1674
Size: 5723
SapI GCTCTTCn'nnn_
Cuts at: 1528 3746 3956 1528
Size: 2218 210 3295
Fragments arranged by size:
3295 2218 210
SbfI CC_TGCA'GG
Cuts at: 2770 2770
Size: 5723
ScaI AGT'ACT
Cuts at: 5240 5240
Size: 5723
SexAI A'CCwGG_T
Cuts at: 239 239
Size: 5723
SfcI C'TryA_G
Cuts at: 33 469 1297 2766 3451 4444 4635 33
Size: 436 828 1469 685 993 191 1121
Fragments arranged by size:
1469 1121 993 828 685 436 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 1624 1624
Size: 5723
SfoI GGC'GCC
Cuts at: 3155 3403 3155
Size: 248 5475
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5723
SmaI CCC'GGG
Cuts at: 325 1031 1876 2745 325
Size: 706 845 869 3303
Fragments arranged by size:
3303 869 845 706
SmlI C'TyrA_G
Cuts at: 1486 2308 2878 3793 4285 4547 1486
Size: 822 570 915 492 262 2662
Fragments arranged by size:
2662 915 822 570 492 262
SnaBI TAC'GTA
Cuts at: 2715 2715
Size: 5723
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5723
SphI G_CATG'C
Cuts at: 2776 3276 2776
Size: 500 5223
StyI C'CwwG_G
Cuts at: 736 2157 2857 3211 736
Size: 1421 700 354 3248
Fragments arranged by size:
3248 1421 700 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 216 1179 1402 1744 2170 2590 3306 3439 3898
Size: 963 223 342 426 420 716 133 459
Cuts at: 3898 4107 216
Size: 209 1832
Fragments arranged by size:
1832 963 716 459 426 420 342 223
209 133
TatI w'GTAC_w
Cuts at: 311 1148 5238 311
Size: 837 4090 796
Fragments arranged by size:
4090 837 796
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2202 2234 5240 5272 2202
Size: 32 3006 32 2653
Fragments arranged by size:
3006 2653 32 32
Tth111I GACn'n_nGTC
Cuts at: 814 3520 814
Size: 2706 3017
XbaI T'CTAG_A
Cuts at: 2754 2754
Size: 5723
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1417 2052 2139 2142 2553 3218 1417
Size: 635 87 3 411 665 3922
Fragments arranged by size:
3922 665 635 411 87 3
XhoI C'TCGA_G
Cuts at: 1486 1486
Size: 5723
XmaI C'CCGG_G
Cuts at: 323 1029 1874 2743 323
Size: 706 845 869 3303
Fragments arranged by size:
3303 869 845 706
XmnI GAAnn'nnTTC
Cuts at: 699 699
Size: 5723
ZraI GAC'GTC
Cuts at: 656 2667 656
Size: 2011 3712
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI
PciI PflMI PfoI PmeI PpiI PpuMI PspXI PstI
PvuI PvuII RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SmaI
SmlI SnaBI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AhdI ApaI AscI AvrII BaeI BbvCI BfrBI
BmtI BsiWI BssHII BstZ17I ClaI EcoRV FseI FspAI
HindIII HpaI MfeI NcoI NdeI NheI NotI NruI
NsiI PacI PasI PmlI PshAI PsiI PspOMI PsrI
SgrAI SrfI SspI StuI SwaI