Restriction map for
pF1KSDB1467
(Circular) (Six-base) MAPSORT of: pF1KSDB1467.seq Check: 2063 from: 1 to: 8039
pF1KSDB1467 8039 bp
With 182 enzymes: SgfI *
June 20, 2008 10:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1315 2199 1315
Size: 884 7155
AccI GT'mk_AC
Cuts at: 320 1328 5077 320
Size: 1008 3749 3282
Fragments arranged by size:
3749 3282 1008
Acc65I G'GTAC_C
Cuts at: 5055 5055
Size: 8039
AclI AA'CG_TT
Cuts at: 7879 7879
Size: 8039
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 903 1279 1726 2336 2337 2770 2923 3019 3754
Size: 376 447 610 1 433 153 96 735
Cuts at: 3754 5864 6296 7043 7311 903
Size: 2110 432 747 268 1631
Fragments arranged by size:
2110 1631 747 735 610 447 433 432
376 268 153 96 1
AflIII A'CryG_T
Cuts at: 3749 5391 6495 7472 3749
Size: 1642 1104 977 4316
Fragments arranged by size:
4316 1642 1104 977
AgeI A'CCGG_T
Cuts at: 6427 6427
Size: 8039
AhdI GACnn_n'nnGTC
Cuts at: 3743 3743
Size: 8039
AleI CACnn'nnGTG
Cuts at: 2238 2238
Size: 8039
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 979 2146 3230 979
Size: 1167 1084 5788
Fragments arranged by size:
5788 1167 1084
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3007 3039 3007
Size: 32 8007
AlwNI CAG_nnn'CTG
Cuts at: 890 1350 1635 2364 2863 2901 3045 3284 5344
Size: 460 285 729 499 38 144 239 2060
Cuts at: 5344 6911 890
Size: 1567 2018
Fragments arranged by size:
2060 2018 1567 729 499 460 285 239
144 38
ApaI G_GGCC'C
Cuts at: 4620 4620
Size: 8039
ApaLI G'TGCA_C
Cuts at: 2432 6809 2432
Size: 4377 3662
ApoI r'AATT_y
Cuts at: 1829 4754 5043 7392 7648 1829
Size: 2925 289 2349 256 2220
Fragments arranged by size:
2925 2349 2220 289 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 8039
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 8039
AvaI C'yCGr_G
Cuts at: 608 1240 1963 2225 4436 4559 5059 608
Size: 632 723 262 2211 123 500 3588
Fragments arranged by size:
3588 2211 723 632 500 262 123
BamHI G'GATC_C
Cuts at: 743 1839 3339 3983 4856 5064 743
Size: 1096 1500 644 873 208 3718
Fragments arranged by size:
3718 1500 1096 873 644 208
BanI G'GyrC_C
Cuts at: 785 975 2310 4579 5020 5055 5469 5717 5752
Size: 190 1335 2269 441 35 414 248 35
Cuts at: 5752 785
Size: 3072
Fragments arranged by size:
3072 2269 1335 441 414 248 190 35
35
BanII G_rGCy'C
Cuts at: 574 1066 1567 2148 2284 2680 2792 4162 4216
Size: 492 501 581 136 396 112 1370 54
Cuts at: 4216 4620 4889 5053 6083 574
Size: 404 269 164 1030 2530
Fragments arranged by size:
2530 1370 1030 581 501 492 404 396
269 164 136 112 54
BbeI G_GCGC'C
Cuts at: 789 5473 5721 789
Size: 4684 248 3107
Fragments arranged by size:
4684 3107 248
BbsI GAAGACnn'nnnn_
Cuts at: 122 1633 2573 3742 3822 4990 122
Size: 1511 940 1169 80 1168 3171
Fragments arranged by size:
3171 1511 1169 1168 940 80
BbvCI CC'TCA_GC
Cuts at: 2098 2098
Size: 8039
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2642 2676 3400 3434 2642
Size: 34 724 34 7247
Fragments arranged by size:
7247 724 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 618 4038 5937 6698 8039 618
Size: 3420 1899 761 1341 618
Fragments arranged by size:
3420 1899 1341 761 618
BclI T'GATC_A
Cuts at: 5559 5559
Size: 8039
BfrBI ATG'CAT
Cuts at: 4698 4698
Size: 8039
BglI GCCn_nnn'nGGC
Cuts at: 1413 2108 3133 1413
Size: 695 1025 6319
Fragments arranged by size:
6319 1025 695
BglII A'GATC_T
Cuts at: 671 2192 3434 4256 5554 7591 671
Size: 1521 1242 822 1298 2037 1119
Fragments arranged by size:
2037 1521 1298 1242 1119 822
BlpI GC'TnA_GC
Cuts at: 2864 4894 5151 2864
Size: 2030 257 5752
Fragments arranged by size:
5752 2030 257
Bme1580I G_kGCm'C
Cuts at: 980 1964 2436 3278 3822 4582 4620 5664 5757
Size: 984 472 842 544 760 38 1044 93
Cuts at: 5757 6813 980
Size: 1056 2206
Fragments arranged by size:
2206 1056 1044 984 842 760 544 472
93 38
BmgBI CAC'GTC
Cuts at: 1386 1386
Size: 8039
BmrI ACTGGGnnnn_n'
Cuts at: 677 802 1838 2197 3245 5251 5332 5667 7811
Size: 125 1036 359 1048 2006 81 335 2144
Cuts at: 7811 677
Size: 905
Fragments arranged by size:
2144 2006 1048 1036 905 359 335 125
81
BmtI G_CTAG'C
Cuts at: 199 199
Size: 8039
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 523 2694 2993 6406 523
Size: 2171 299 3413 2156
Fragments arranged by size:
3413 2171 2156 299
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 187 518 1230 1438 2540 3214 4024 4705 4873
Size: 331 712 208 1102 674 810 681 168
Cuts at: 4873 187
Size: 3353
Fragments arranged by size:
3353 1102 810 712 681 674 331 208
168
Bpu10I CC'TnA_GC
Cuts at: 2098 4976 2098
Size: 2878 5161
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 343 2988 2995 3997 4192 5215 6094 6586 6884
Size: 2645 7 1002 195 1023 879 492 298
Cuts at: 6884 343
Size: 1498
Fragments arranged by size:
2645 1498 1023 1002 879 492 298 195
7
BsaI GGTCTCn'nnnn_
Cuts at: 379 1113 3526 7743 379
Size: 734 2413 4217 675
Fragments arranged by size:
4217 2413 734 675
BsaAI yAC'GTr
Cuts at: 3689 5031 5392 3689
Size: 1342 361 6336
Fragments arranged by size:
6336 1342 361
BsaBI GATnn'nnATC
Cuts at: 6 3421 5553 7590 6
Size: 3415 2132 2037 455
Fragments arranged by size:
3415 2132 2037 455
BsaHI Gr'CG_yC
Cuts at: 786 1024 5470 5718 6420 7916 786
Size: 238 4446 248 702 1496 909
Fragments arranged by size:
4446 1496 909 702 248 238
BsaWI w'CCGG_w
Cuts at: 5228 5749 6427 6701 6848 7259 5228
Size: 521 678 274 147 411 6008
Fragments arranged by size:
6008 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 430 460 1303 1333 3283 3313 3616 3646 4433
Size: 30 843 30 1950 30 303 30 787
Cuts at: 4433 4463 4631 4661 430
Size: 30 168 30 3808
Fragments arranged by size:
3808 1950 843 787 303 168 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 274 587 1141 1435 2274 3211 4360 4483 4924
Size: 313 554 294 839 937 1149 123 441
Cuts at: 4924 274
Size: 3389
Fragments arranged by size:
3389 1149 937 839 554 441 313 294
123
BseYI C'CCAG_C
Cuts at: 302 677 765 2327 2416 2707 2711 2885 3137
Size: 375 88 1562 89 291 4 174 252
Cuts at: 3137 4402 4587 5464 6222 6799 7295 302
Size: 1265 185 877 758 577 496 1046
Fragments arranged by size:
1562 1265 1046 877 758 577 496 375
291 252 185 174 89 88 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2142 2227 2857 2142
Size: 85 630 7324
Fragments arranged by size:
7324 630 85
BsiEI CG_ry'CG
Cuts at: 104 3495 4431 5581 5627 6835 104
Size: 3391 936 1150 46 1208 1308
Fragments arranged by size:
3391 1308 1208 1150 936 46
BsiHKAI G_wGCw'C
Cuts at: 1238 1788 2148 2284 2436 2680 2696 4216 4889
Size: 550 360 136 152 244 16 1520 673
Cuts at: 4889 5053 5831 6021 6813 1238
Size: 164 778 190 792 2464
Fragments arranged by size:
2464 1520 792 778 673 550 360 244
190 164 152 136 16
BsiWI C'GTAC_G
Cuts at: 3689 3689
Size: 8039
BsmI GAATG_Cn'
Cuts at: 1023 2917 1023
Size: 1894 6145
Bsp1286I G_dGCh'C
Cuts at: 574 980 1066 1238 1567 1788 1964 2148 2284
Size: 406 86 172 329 221 176 184 136
Cuts at: 2284 2436 2680 2696 2792 3278 3822 4162 4216
Size: 152 244 16 96 486 544 340 54
Cuts at: 4216 4582 4620 4889 5053 5664 5757 5831 6021
Size: 366 38 269 164 611 93 74 190
Cuts at: 6021 6083 6813 574
Size: 62 730 1800
Fragments arranged by size:
1800 730 611 544 486 406 366 340
329 269 244 221 190 184 176 172
164 152 136 96 93 86 74 62
54 38 16
BspEI T'CCGG_A
Cuts at: 7259 7259
Size: 8039
BspHI T'CATG_A
Cuts at: 1455 3425 7215 1455
Size: 1970 3790 2279
Fragments arranged by size:
3790 2279 1970
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1315 2199 2349 2888 3132 4825 4880 5089 5303
Size: 884 150 539 244 1693 55 209 214
Cuts at: 5303 5605 5986 1315
Size: 302 381 3368
Fragments arranged by size:
3368 1693 884 539 381 302 244 214
209 150 55
BsrBI CCG'CTC
Cuts at: 50 4433 4458 6331 6385 7869 8034 50
Size: 4383 25 1873 54 1484 165 55
Fragments arranged by size:
4383 1873 1484 165 55 54 25
BsrDI GCAATG_nn'
Cuts at: 150 5951 150
Size: 5801 2238
BsrFI r'CCGG_y
Cuts at: 2216 3490 3568 3584 4169 4987 6037 6218 6427
Size: 1274 78 16 585 818 1050 181 209
Cuts at: 6427 2216
Size: 3828
Fragments arranged by size:
3828 1274 1050 818 585 209 181 78
16
BsrGI T'GTAC_A
Cuts at: 4535 4535
Size: 8039
BssSI C'ACGA_G
Cuts at: 3228 4315 6310 6668 3228
Size: 1087 1995 358 4599
Fragments arranged by size:
4599 1995 1087 358
BstAPI GCAn_nnn'nTGC
Cuts at: 5416 5416
Size: 8039
BstBI TT'CG_AA
Cuts at: 110 6400 110
Size: 6290 1749
BstEII G'GTnAC_C
Cuts at: 3564 4867 3564
Size: 1303 6736
BstXI CCAn_nnnn'nTGG
Cuts at: 1851 2016 3258 3796 1851
Size: 165 1242 538 6094
Fragments arranged by size:
6094 1242 538 165
BstYI r'GATC_y
Cuts at: 1 471 671 743 1839 2192 3339 3434 3545
Size: 470 200 72 1096 353 1147 95 111
Cuts at: 3545 3983 4256 4856 5005 5064 5531 5554 5889
Size: 438 273 600 149 59 467 23 335
Cuts at: 5889 6135 7147 7233 7245 7591 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1147 1096 1012 600 470 467 449 438
353 346 335 273 246 200 149 111
95 86 72 59 23 12
Bsu36I CC'TnA_GG
Cuts at: 971 1372 4033 4040 4511 971
Size: 401 2661 7 471 4499
Fragments arranged by size:
4499 2661 471 401 7
BtgI C'CryG_G
Cuts at: 944 1760 2103 944
Size: 816 343 6880
Fragments arranged by size:
6880 816 343
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 214 5989 6170 6333 214
Size: 5775 181 163 1920
Fragments arranged by size:
5775 1920 181 163
BtsI GCAGTG_nn'
Cuts at: 1536 1964 2233 2761 2845 4107 4552 5282 1536
Size: 428 269 528 84 1262 445 730 4293
Fragments arranged by size:
4293 1262 730 528 445 428 269 84
DraI TTT'AAA
Cuts at: 5038 5038
Size: 8039
DrdI GACnn_nn'nnGTC
Cuts at: 1720 5745 6603 1720
Size: 4025 858 3156
Fragments arranged by size:
4025 3156 858
EaeI y'GGCC_r
Cuts at: 203 947 1089 1754 2373 2395 3125 3492 3683
Size: 744 142 665 619 22 730 367 191
Cuts at: 3683 3768 4218 4428 4451 4517 5624 5798 6189
Size: 85 450 210 23 66 1107 174 391
Cuts at: 6189 6216 7561 203
Size: 27 1345 681
Fragments arranged by size:
1345 1107 744 730 681 665 619 450
391 367 210 191 174 142 85 66
27 23 22
EagI C'GGCC_G
Cuts at: 3492 4428 5624 3492
Size: 936 1196 5907
Fragments arranged by size:
5907 1196 936
EarI CTCTTCn'nnn_
Cuts at: 546 746 752 1109 2136 2170 2350 3083 3952
Size: 200 6 357 1027 34 180 733 869
Cuts at: 3952 4387 4687 6062 6272 546
Size: 435 300 1375 210 2313
Fragments arranged by size:
2313 1375 1027 869 733 435 357 300
210 200 180 34 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 363 4476 4942 6247 6557 6703 7849 363
Size: 4113 466 1305 310 146 1146 553
Fragments arranged by size:
4113 1305 1146 553 466 310 146
EcoICRI GAG'CTC
Cuts at: 2146 2282 2678 4214 4887 5051 2146
Size: 136 396 1536 673 164 5134
Fragments arranged by size:
5134 1536 673 396 164 136
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 187 518 903 1230 1279 1438 1726 2336 2337
Size: 331 385 327 49 159 288 610 1
Cuts at: 2337 2540 2770 2923 3019 3214 3754 4024 4705
Size: 203 230 153 96 195 540 270 681
Cuts at: 4705 4873 5864 6296 7043 7311 187
Size: 168 991 432 747 268 915
Fragments arranged by size:
991 915 747 681 610 540 432 385
331 327 288 270 268 230 203 195
168 159 153 96 49 1
EcoNI CCTnn'n_nnAGG
Cuts at: 751 1987 3196 3391 4939 4975 751
Size: 1236 1209 195 1548 36 3815
Fragments arranged by size:
3815 1548 1236 1209 195 36
EcoO109I rG'GnC_Cy
Cuts at: 653 1632 2012 2361 2898 4008 5178 653
Size: 979 380 349 537 1110 1170 3514
Fragments arranged by size:
3514 1170 1110 979 537 380 349
EcoRI G'AATT_C
Cuts at: 5043 5043
Size: 8039
EcoRV GAT'ATC
Cuts at: 1158 1158
Size: 8039
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1461 2181 3228 6058 1461
Size: 720 1047 2830 3442
Fragments arranged by size:
3442 2830 1047 720
FspI TGC'GCA
Cuts at: 1528 3463 5820 1528
Size: 1935 2357 3747
Fragments arranged by size:
3747 2357 1935
FspAI rTGC'GCAy
Cuts at: 3463 3463
Size: 8039
HaeII r_GCGC'y
Cuts at: 789 2423 2640 2705 5473 5721 6743 7722 789
Size: 1634 217 65 2768 248 1022 979 1106
Fragments arranged by size:
2768 1634 1106 1022 979 248 217 65
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 220 252 602 634 1862 1894 3729 3761 5881
Size: 32 350 32 1228 32 1835 32 2120
Cuts at: 5881 5911 5913 5943 220
Size: 30 2 30 2316
Fragments arranged by size:
2316 2120 1835 1228 350 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1254 3900 5078 1254
Size: 2646 1178 4215
Fragments arranged by size:
4215 2646 1178
HindIII A'AGCT_T
Cuts at: 2380 2578 2380
Size: 198 7841
KasI G'GCGC_C
Cuts at: 785 5469 5717 785
Size: 4684 248 3107
Fragments arranged by size:
4684 3107 248
KpnI G_GTAC'C
Cuts at: 5059 5059
Size: 8039
MfeI C'AATT_G
Cuts at: 715 715
Size: 8039
MluI A'CGCG_T
Cuts at: 7472 7472
Size: 8039
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2407 4608 6710 6894 2407
Size: 2201 2102 184 3552
Fragments arranged by size:
3552 2201 2102 184
MscI TGG'CCA
Cuts at: 949 1091 2397 3127 3685 5800 949
Size: 142 1306 730 558 2115 3188
Fragments arranged by size:
3188 2115 1306 730 558 142
MslI CAynn'nnrTG
Cuts at: 2238 2777 3463 4690 4693 6028 6155 2238
Size: 539 686 1227 3 1335 127 4122
Fragments arranged by size:
4122 1335 1227 686 539 127 3
MspA1I CmG'CkG
Cuts at: 302 912 1506 1797 1897 2304 2711 2889 3479
Size: 610 594 291 100 407 407 178 590
Cuts at: 3479 4335 4881 4893 5150 5464 5824 6837 7082
Size: 856 546 12 257 314 360 1013 245
Cuts at: 7082 302
Size: 1259
Fragments arranged by size:
1259 1013 856 610 594 590 546 407
407 360 314 291 257 245 178 100
12
NaeI GCC'GGC
Cuts at: 6220 6220
Size: 8039
NarI GG'CG_CC
Cuts at: 786 5470 5718 786
Size: 4684 248 3107
Fragments arranged by size:
4684 3107 248
NcoI C'CATG_G
Cuts at: 2103 2103
Size: 8039
NgoMIV G'CCGG_C
Cuts at: 6218 6218
Size: 8039
NheI G'CTAG_C
Cuts at: 195 195
Size: 8039
NsiI A_TGCA'T
Cuts at: 4700 4700
Size: 8039
NspI r_CATG'y
Cuts at: 1152 1444 1821 2800 3402 3462 3753 5092 5592
Size: 292 377 979 602 60 291 1339 500
Cuts at: 5592 6499 1152
Size: 907 2692
Fragments arranged by size:
2692 1339 979 907 602 500 377 292
291 60
PasI CC'CwG_GG
Cuts at: 2322 2784 3278 3345 3619 5001 2322
Size: 462 494 67 274 1382 5360
Fragments arranged by size:
5360 1382 494 462 274 67
PciI A'CATG_T
Cuts at: 3749 6495 3749
Size: 2746 5293
PflMI CCAn_nnn'nTGG
Cuts at: 463 890 1152 2057 3336 4137 463
Size: 427 262 905 1279 801 4365
Fragments arranged by size:
4365 1279 905 801 427 262
PfoI T'CCnGG_A
Cuts at: 1988 2558 2807 3146 3443 4352 4721 1988
Size: 570 249 339 297 909 369 5306
Fragments arranged by size:
5306 909 570 369 339 297 249
PmeI GTTT'AAAC
Cuts at: 5038 5038
Size: 8039
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4188 4220 7178 7210 4188
Size: 32 2958 32 5017
Fragments arranged by size:
5017 2958 32 32
PpuMI rG'GwC_Cy
Cuts at: 653 2012 2361 4008 653
Size: 1359 349 1647 4684
Fragments arranged by size:
4684 1647 1359 349
PspOMI G'GGCC_C
Cuts at: 4616 4616
Size: 8039
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3643 3675 3643
Size: 32 8007
PstI C_TGCA'G
Cuts at: 218 1053 1433 2135 2471 3413 3608 4877 5086
Size: 835 380 702 336 942 195 1269 209
Cuts at: 5086 5771 218
Size: 685 2486
Fragments arranged by size:
2486 1269 942 835 702 685 380 336
209 195
PvuI CG_AT'CG
Cuts at: 104 104
Size: 8039
PvuII CAG'CTG
Cuts at: 302 1506 1797 2711 2889 4893 5464 5824 302
Size: 1204 291 914 178 2004 571 360 2517
Fragments arranged by size:
2517 2004 1204 914 571 360 291 178
RsrII CG'GwC_CG
Cuts at: 350 6234 350
Size: 5884 2155
SacI G_AGCT'C
Cuts at: 2148 2284 2680 4216 4889 5053 2148
Size: 136 396 1536 673 164 5134
Fragments arranged by size:
5134 1536 673 396 164 136
SalI G'TCGA_C
Cuts at: 5076 5076
Size: 8039
SanDI GG'GwC_CC
Cuts at: 653 653
Size: 8039
SapI GCTCTTCn'nnn_
Cuts at: 2136 4387 6062 6272 2136
Size: 2251 1675 210 3903
Fragments arranged by size:
3903 2251 1675 210
SbfI CC_TGCA'GG
Cuts at: 5086 5086
Size: 8039
ScaI AGT'ACT
Cuts at: 7556 7556
Size: 8039
SexAI A'CCwGG_T
Cuts at: 1910 1982 1910
Size: 72 7967
SfcI C'TryA_G
Cuts at: 33 214 1049 1325 1429 1501 2131 2467 3409
Size: 181 835 276 104 72 630 336 942
Cuts at: 3409 3604 4873 5082 5767 6760 6951 33
Size: 195 1269 209 685 993 191 1121
Fragments arranged by size:
1269 1121 993 942 835 685 630 336
276 209 195 191 181 104 72
SfiI GGCCn_nnn'nGGCC
Cuts at: 3133 3133
Size: 8039
SfoI GGC'GCC
Cuts at: 787 5471 5719 787
Size: 4684 248 3107
Fragments arranged by size:
4684 3107 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 8039
SmaI CCC'GGG
Cuts at: 1965 4438 4561 5061 1965
Size: 2473 123 500 4943
Fragments arranged by size:
4943 2473 500 123
SmlI C'TyrA_G
Cuts at: 358 2967 3010 4012 4207 5194 6109 6601 6863
Size: 2609 43 1002 195 987 915 492 262
Cuts at: 6863 358
Size: 1534
Fragments arranged by size:
2609 1534 1002 987 915 492 262 195
43
SnaBI TAC'GTA
Cuts at: 5031 5031
Size: 8039
SpeI A'CTAG_T
Cuts at: 67 67
Size: 8039
SphI G_CATG'C
Cuts at: 1444 1821 2800 5092 5592 1444
Size: 377 979 2292 500 3891
Fragments arranged by size:
3891 2292 979 500 377
StuI AGG'CCT
Cuts at: 1605 1996 4038 1605
Size: 391 2042 5606
Fragments arranged by size:
5606 2042 391
StyI C'CwwG_G
Cuts at: 450 1445 2103 4862 5173 5527 450
Size: 995 658 2759 311 354 2962
Fragments arranged by size:
2962 2759 995 658 354 311
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 367 1375 1378 2053 3337 3725 3955 4565 5622
Size: 1008 3 675 1284 388 230 610 1057
Cuts at: 5622 5755 6214 6423 367
Size: 133 459 209 1983
Fragments arranged by size:
1983 1284 1057 1008 675 610 459 388
230 209 133 3
TatI w'GTAC_w
Cuts at: 1460 1922 2609 4535 7554 1460
Size: 462 687 1926 3019 1945
Fragments arranged by size:
3019 1945 1926 687 462
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 145 177 430 462 929 961 2426 2458 4433
Size: 32 253 32 467 32 1465 32 1975
Cuts at: 4433 4465 4631 4663 7556 7588 145
Size: 32 166 32 2893 32 596
Fragments arranged by size:
2893 1975 1465 596 467 253 166 32
32 32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1984 2458 5836 1984
Size: 474 3378 4187
Fragments arranged by size:
4187 3378 474
XbaI T'CTAG_A
Cuts at: 5070 5070
Size: 8039
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1908 3556 3693 3763 5534 1908
Size: 1648 137 70 1771 4413
Fragments arranged by size:
4413 1771 1648 137 70
XmaI C'CCGG_G
Cuts at: 1963 4436 4559 5059 1963
Size: 2473 123 500 4943
Fragments arranged by size:
4943 2473 500 123
XmnI GAAnn'nnTTC
Cuts at: 3208 4258 3208
Size: 1050 6989
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI FspAI HaeII Hin4I HincII
HindIII KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NheI NsiI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PspOMI
PsrI PstI PvuI PvuII RsrII SacI SalI SanDI
SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI
SmaI SmlI SnaBI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AscI AvrII BaeI BsmBI BssHII
BstZ17I ClaI CspCI DraIII FseI HpaI NdeI NotI
NruI PacI PmlI PshAI PsiI PspXI SacII SgrAI
SrfI SspI SwaI XhoI ZraI