Restriction map for
pF1KSDB1480
(Circular) (Six-base) MAPSORT of: pF1KSDB1480.seq Check: 4018 from: 1 to: 6095
pF1KSDB1480 6095 bp
With 182 enzymes: SgfI *
June 20, 2008 10:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1989 1989
Size: 6095
AatII G_ACGT'C
Cuts at: 2957 2957
Size: 6095
AccI GT'mk_AC
Cuts at: 1196 3133 1196
Size: 1937 4158
Acc65I G'GTAC_C
Cuts at: 3111 3111
Size: 6095
AclI AA'CG_TT
Cuts at: 5935 5935
Size: 6095
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1037 2065 3920 4352 5099 5367 1037
Size: 1028 1855 432 747 268 1765
Fragments arranged by size:
1855 1765 1028 747 432 268
AflIII A'CryG_T
Cuts at: 314 3447 4551 5528 314
Size: 3133 1104 977 881
Fragments arranged by size:
3133 1104 977 881
AgeI A'CCGG_T
Cuts at: 4483 4483
Size: 6095
AhdI GACnn_n'nnGTC
Cuts at: 2958 2958
Size: 6095
AleI CACnn'nnGTG
Cuts at: 610 2206 610
Size: 1596 4499
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2845 2845
Size: 6095
AlwNI CAG_nnn'CTG
Cuts at: 214 3400 4967 214
Size: 3186 1567 1342
Fragments arranged by size:
3186 1567 1342
ApaLI G'TGCA_C
Cuts at: 4865 4865
Size: 6095
ApoI r'AATT_y
Cuts at: 3099 5448 5704 3099
Size: 2349 256 3490
Fragments arranged by size:
3490 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6095
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6095
AvaI C'yCGr_G
Cuts at: 791 1224 2679 3115 791
Size: 433 1455 436 3771
Fragments arranged by size:
3771 1455 436 433
BamHI G'GATC_C
Cuts at: 3120 3120
Size: 6095
BanI G'GyrC_C
Cuts at: 362 370 736 870 922 1234 1627 2805 3111
Size: 8 366 134 52 312 393 1178 306
Cuts at: 3111 3525 3773 3808 362
Size: 414 248 35 2649
Fragments arranged by size:
2649 1178 414 393 366 312 306 248
134 52 35 8
BanII G_rGCy'C
Cuts at: 1381 1559 2426 3109 4139 1381
Size: 178 867 683 1030 3337
Fragments arranged by size:
3337 1030 867 683 178
BbeI G_GCGC'C
Cuts at: 366 374 740 1631 2809 3529 3777 366
Size: 8 366 891 1178 720 248 2684
Fragments arranged by size:
2684 1178 891 720 366 248 8
BbsI GAAGACnn'nnnn_
Cuts at: 693 1632 1776 693
Size: 939 144 5012
Fragments arranged by size:
5012 939 144
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 786 820 1415 1449 2364 2398 2635 2669 2670
Size: 34 595 34 915 34 237 34 1
Cuts at: 2670 2704 786
Size: 34 4177
Fragments arranged by size:
4177 915 595 237 34 34 34 34
34 1
BciVI GTATCCnnnnn_n'
Cuts at: 534 3046 3993 4754 6095 534
Size: 2512 947 761 1341 534
Fragments arranged by size:
2512 1341 947 761 534
BclI T'GATC_A
Cuts at: 2213 3615 2213
Size: 1402 4693
BfrBI ATG'CAT
Cuts at: 1452 1452
Size: 6095
BglI GCCn_nnn'nGGC
Cuts at: 879 1046 1283 879
Size: 167 237 5691
Fragments arranged by size:
5691 237 167
BglII A'GATC_T
Cuts at: 1907 2066 3610 5647 1907
Size: 159 1544 2037 2355
Fragments arranged by size:
2355 2037 1544 159
BlpI GC'TnA_GC
Cuts at: 218 3207 218
Size: 2989 3106
Bme1580I G_kGCm'C
Cuts at: 718 875 1656 2475 3720 3813 4869 718
Size: 157 781 819 1245 93 1056 1944
Fragments arranged by size:
1944 1245 1056 819 781 157 93
BmrI ACTGGGnnnn_n'
Cuts at: 1326 2616 3307 3388 3723 5867 1326
Size: 1290 691 81 335 2144 1554
Fragments arranged by size:
2144 1554 1290 691 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 582 1207 2256 4462 582
Size: 625 1049 2206 2215
Fragments arranged by size:
2215 2206 1049 625
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1171 2200 2911 1171
Size: 1029 711 4355
Fragments arranged by size:
4355 1029 711
Bpu10I CC'TnA_GC
Cuts at: 2128 2128
Size: 6095
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2074 3271 4150 4642 4940 2074
Size: 1197 879 492 298 3229
Fragments arranged by size:
3229 1197 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 445 1129 5799 445
Size: 684 4670 741
Fragments arranged by size:
4670 741 684
BsaAI yAC'GTr
Cuts at: 299 387 3087 3448 299
Size: 88 2700 361 2946
Fragments arranged by size:
2946 2700 361 88
BsaBI GATnn'nnATC
Cuts at: 6 1912 2215 2491 3609 5646 6
Size: 1906 303 276 1118 2037 455
Fragments arranged by size:
2037 1906 1118 455 303 276
BsaHI Gr'CG_yC
Cuts at: 363 371 737 1628 2806 2954 3526 3774 4476
Size: 8 366 891 1178 148 572 248 702
Cuts at: 4476 5972 363
Size: 1496 486
Fragments arranged by size:
1496 1178 891 702 572 486 366 248
148 8
BsaWI w'CCGG_w
Cuts at: 1174 2525 3284 3805 4483 4757 4904 5315 1174
Size: 1351 759 521 678 274 147 411 1954
Fragments arranged by size:
1954 1351 759 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 382 412 2569 2599 382
Size: 30 2157 30 3878
Fragments arranged by size:
3878 2157 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1755 2805 1755
Size: 1050 5045
BseYI C'CCAG_C
Cuts at: 141 222 427 1496 3520 4278 4855 5351 141
Size: 81 205 1069 2024 758 577 496 885
Fragments arranged by size:
2024 1069 885 758 577 496 205 81
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1071 1224 1630 2674 1071
Size: 153 406 1044 4492
Fragments arranged by size:
4492 1044 406 153
BsiEI CG_ry'CG
Cuts at: 104 1772 3637 3683 4891 104
Size: 1668 1865 46 1208 1308
Fragments arranged by size:
1865 1668 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 775 1559 2203 3109 3887 4077 4869 775
Size: 784 644 906 778 190 792 2001
Fragments arranged by size:
2001 906 792 784 778 644 190
Bsp1286I G_dGCh'C
Cuts at: 718 775 875 1381 1559 1656 2203 2426 2475
Size: 57 100 506 178 97 547 223 49
Cuts at: 2475 3109 3720 3813 3887 4077 4139 4869 718
Size: 634 611 93 74 190 62 730 1944
Fragments arranged by size:
1944 730 634 611 547 506 223 190
178 100 97 93 74 62 57 49
BspEI T'CCGG_A
Cuts at: 1174 5315 1174
Size: 4141 1954
BspHI T'CATG_A
Cuts at: 593 5271 593
Size: 4678 1417
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1325 1989 2837 3145 3359 3661 4042 1325
Size: 664 848 308 214 302 381 3378
Fragments arranged by size:
3378 848 664 381 308 302 214
BsrBI CCG'CTC
Cuts at: 50 2598 4387 4441 5925 6090 50
Size: 2548 1789 54 1484 165 55
Fragments arranged by size:
2548 1789 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 545 1050 1790 4007 545
Size: 505 740 2217 2633
Fragments arranged by size:
2633 2217 740 505
BsrFI r'CCGG_y
Cuts at: 1624 2732 2935 4093 4274 4483 1624
Size: 1108 203 1158 181 209 3236
Fragments arranged by size:
3236 1158 1108 209 203 181
BsrGI T'GTAC_A
Cuts at: 1260 1260
Size: 6095
BssSI C'ACGA_G
Cuts at: 4366 4724 4366
Size: 358 5737
BstAPI GCAn_nnn'nTGC
Cuts at: 929 1247 3472 929
Size: 318 2225 3552
Fragments arranged by size:
3552 2225 318
BstBI TT'CG_AA
Cuts at: 110 4456 110
Size: 4346 1749
BstEII G'GTnAC_C
Cuts at: 1602 1602
Size: 6095
BstXI CCAn_nnnn'nTGG
Cuts at: 251 516 667 1513 2475 2904 251
Size: 265 151 846 962 429 3442
Fragments arranged by size:
3442 962 846 429 265 151
BstYI r'GATC_y
Cuts at: 1 1346 1907 2066 2486 3120 3587 3610 3945
Size: 1345 561 159 420 634 467 23 335
Cuts at: 3945 4191 5203 5289 5301 5647 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1345 1012 634 561 467 449 420 346
335 246 159 86 23 12
BtgI C'CryG_G
Cuts at: 125 179 366 526 1469 1661 2669 2801 125
Size: 54 187 160 943 192 1008 132 3419
Fragments arranged by size:
3419 1008 943 192 187 160 132 54
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 672 761 789 1791 2298 4045 4226 4389 672
Size: 89 28 1002 507 1747 181 163 2378
Fragments arranged by size:
2378 1747 1002 507 181 163 89 28
BtsI GCAGTG_nn'
Cuts at: 1445 1955 2040 3338 1445
Size: 510 85 1298 4202
Fragments arranged by size:
4202 1298 510 85
ClaI AT'CG_AT
Cuts at: 1916 1916
Size: 6095
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1200 1235 1200
Size: 35 6060
DraI TTT'AAA
Cuts at: 3094 3094
Size: 6095
DrdI GACnn_nn'nnGTC
Cuts at: 2091 3801 4659 2091
Size: 1710 858 3527
Fragments arranged by size:
3527 1710 858
EaeI y'GGCC_r
Cuts at: 491 585 1769 1884 2037 2075 2540 2745 3009
Size: 94 1184 115 153 38 465 205 264
Cuts at: 3009 3680 3854 4245 4272 5617 491
Size: 671 174 391 27 1345 969
Fragments arranged by size:
1345 1184 969 671 465 391 264 205
174 153 115 94 38 27
EagI C'GGCC_G
Cuts at: 1769 3680 1769
Size: 1911 4184
EarI CTCTTCn'nnn_
Cuts at: 251 812 4118 4328 251
Size: 561 3306 210 2018
Fragments arranged by size:
3306 2018 561 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2495 4303 4613 4759 5905 2495
Size: 1808 310 146 1146 2685
Fragments arranged by size:
2685 1808 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1557 3107 1557
Size: 1550 4545
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1037 1171 2065 2200 2911 3920 4352 5099 5367
Size: 134 894 135 711 1009 432 747 268
Cuts at: 5367 1037
Size: 1765
Fragments arranged by size:
1765 1009 894 747 711 432 268 135
134
EcoNI CCTnn'n_nnAGG
Cuts at: 245 245
Size: 6095
EcoO109I rG'GnC_Cy
Cuts at: 1188 1614 1935 3234 1188
Size: 426 321 1299 4049
Fragments arranged by size:
4049 1299 426 321
EcoRI G'AATT_C
Cuts at: 3099 3099
Size: 6095
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 471 1713 2940 4114 471
Size: 1242 1227 1174 2452
Fragments arranged by size:
2452 1242 1227 1174
FspI TGC'GCA
Cuts at: 3876 3876
Size: 6095
HaeII r_GCGC'y
Cuts at: 366 374 740 885 1631 1680 2118 2687 2809
Size: 8 366 145 746 49 438 569 122
Cuts at: 2809 3529 3777 4799 5778 366
Size: 720 248 1022 979 683
Fragments arranged by size:
1022 979 746 720 683 569 438 366
248 145 122 49 8
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 222 254 472 504 507 539 760 792 2434
Size: 32 218 32 3 32 221 32 1642
Cuts at: 2434 2466 2569 2601 2766 2798 2944 2976 3937
Size: 32 103 32 165 32 146 32 961
Cuts at: 3937 3967 3969 3999 222
Size: 30 2 30 2318
Fragments arranged by size:
2318 1642 961 221 218 165 146 103
32 32 32 32 32 32 32 32
30 30 3 2
HincII GTy'rAC
Cuts at: 2388 2757 3134 2388
Size: 369 377 5349
Fragments arranged by size:
5349 377 369
KasI G'GCGC_C
Cuts at: 362 370 736 1627 2805 3525 3773 362
Size: 8 366 891 1178 720 248 2684
Fragments arranged by size:
2684 1178 891 720 366 248 8
KpnI G_GTAC'C
Cuts at: 3115 3115
Size: 6095
MluI A'CGCG_T
Cuts at: 5528 5528
Size: 6095
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2393 4766 4950 2393
Size: 2373 184 3538
Fragments arranged by size:
3538 2373 184
MscI TGG'CCA
Cuts at: 1886 2039 2077 3011 3856 1886
Size: 153 38 934 845 4125
Fragments arranged by size:
4125 934 845 153 38
MslI CAynn'nnrTG
Cuts at: 249 385 610 691 1795 2206 2569 2902 4084
Size: 136 225 81 1104 411 363 333 1182
Cuts at: 4084 4211 249
Size: 127 2133
Fragments arranged by size:
2133 1182 1104 411 363 333 225 136
127 81
MspA1I CmG'CkG
Cuts at: 168 641 800 1247 1685 1774 2292 2320 2498
Size: 473 159 447 438 89 518 28 178
Cuts at: 2498 2835 3206 3520 3880 4893 5138 168
Size: 337 371 314 360 1013 245 1125
Fragments arranged by size:
1125 1013 518 473 447 438 371 360
337 314 245 178 159 89 28
NaeI GCC'GGC
Cuts at: 1626 2937 4276 1626
Size: 1311 1339 3445
Fragments arranged by size:
3445 1339 1311
NarI GG'CG_CC
Cuts at: 363 371 737 1628 2806 3526 3774 363
Size: 8 366 891 1178 720 248 2684
Fragments arranged by size:
2684 1178 891 720 366 248 8
NcoI C'CATG_G
Cuts at: 125 526 2669 2801 125
Size: 401 2143 132 3419
Fragments arranged by size:
3419 2143 401 132
NgoMIV G'CCGG_C
Cuts at: 1624 2935 4274 1624
Size: 1311 1339 3445
Fragments arranged by size:
3445 1339 1311
NsiI A_TGCA'T
Cuts at: 1454 1454
Size: 6095
NspI r_CATG'y
Cuts at: 681 891 1083 2304 3148 3648 4555 681
Size: 210 192 1221 844 500 907 2221
Fragments arranged by size:
2221 1221 907 844 500 210 192
PasI CC'CwG_GG
Cuts at: 2475 2475
Size: 6095
PciI A'CATG_T
Cuts at: 4551 4551
Size: 6095
PflMI CCAn_nnn'nTGG
Cuts at: 2295 2498 2295
Size: 203 5892
PfoI T'CCnGG_A
Cuts at: 659 746 1523 1736 659
Size: 87 777 213 5018
Fragments arranged by size:
5018 777 213 87
PmeI GTTT'AAAC
Cuts at: 3094 3094
Size: 6095
PmlI CAC'GTG
Cuts at: 387 387
Size: 6095
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 114 146 5234 5266 114
Size: 32 5088 32 943
Fragments arranged by size:
5088 943 32 32
PpuMI rG'GwC_Cy
Cuts at: 1188 1935 1188
Size: 747 5348
PshAI GACnn'nnGTC
Cuts at: 630 630
Size: 6095
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2515 2547 2515
Size: 32 6063
PstI C_TGCA'G
Cuts at: 418 1541 2035 3142 3827 418
Size: 1123 494 1107 685 2686
Fragments arranged by size:
2686 1123 1107 685 494
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6095
PvuII CAG'CTG
Cuts at: 800 2292 2320 3520 3880 800
Size: 1492 28 1200 360 3015
Fragments arranged by size:
3015 1492 1200 360 28
RsrII CG'GwC_CG
Cuts at: 4290 4290
Size: 6095
SacI G_AGCT'C
Cuts at: 1559 3109 1559
Size: 1550 4545
SalI G'TCGA_C
Cuts at: 3132 3132
Size: 6095
SapI GCTCTTCn'nnn_
Cuts at: 4118 4328 4118
Size: 210 5885
SbfI CC_TGCA'GG
Cuts at: 3142 3142
Size: 6095
ScaI AGT'ACT
Cuts at: 1543 5612 1543
Size: 4069 2026
SexAI A'CCwGG_T
Cuts at: 302 2396 302
Size: 2094 4001
SfcI C'TryA_G
Cuts at: 33 206 414 1053 1537 1680 2031 3138 3823
Size: 173 208 639 484 143 351 1107 685
Cuts at: 3823 4816 5007 33
Size: 993 191 1121
Fragments arranged by size:
1121 1107 993 685 639 484 351 208
191 173 143
SfoI GGC'GCC
Cuts at: 364 372 738 1629 2807 3527 3775 364
Size: 8 366 891 1178 720 248 2684
Fragments arranged by size:
2684 1178 891 720 366 248 8
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6095
SmaI CCC'GGG
Cuts at: 793 3117 793
Size: 2324 3771
SmlI C'TyrA_G
Cuts at: 2089 3250 4165 4657 4919 2089
Size: 1161 915 492 262 3265
Fragments arranged by size:
3265 1161 915 492 262
SnaBI TAC'GTA
Cuts at: 3087 3087
Size: 6095
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6095
SphI G_CATG'C
Cuts at: 2304 3148 3648 2304
Size: 844 500 4751
Fragments arranged by size:
4751 844 500
StuI AGG'CCT
Cuts at: 979 1286 979
Size: 307 5788
StyI C'CwwG_G
Cuts at: 125 526 966 1964 2669 2801 3229 3583 125
Size: 401 440 998 705 132 428 354 2637
Fragments arranged by size:
2637 998 705 440 428 401 354 132
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 519 571 1306 1345 3678 3811 4270 4479 519
Size: 52 735 39 2333 133 459 209 2135
Fragments arranged by size:
2333 2135 735 459 209 133 52 39
TatI w'GTAC_w
Cuts at: 1260 1334 1541 1565 1694 1853 2912 5610 1260
Size: 74 207 24 129 159 1059 2698 1745
Fragments arranged by size:
2698 1745 1059 207 159 129 74 24
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 847 879 5612 5644 847
Size: 32 4733 32 1298
Fragments arranged by size:
4733 1298 32 32
Tth111I GACn'n_nGTC
Cuts at: 1192 3892 1192
Size: 2700 3395
XbaI T'CTAG_A
Cuts at: 2436 3126 2436
Size: 690 5405
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3590 3590
Size: 6095
XmaI C'CCGG_G
Cuts at: 791 3115 791
Size: 2324 3771
ZraI GAC'GTC
Cuts at: 2955 2955
Size: 6095
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NsiI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsrI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SnaBI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AfeI AflII AloI ApaI AscI AvrII BaeI BbvCI
BmgBI BmtI BsiWI BsmI BsmBI BssHII BstZ17I Bsu36I
DraIII EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI PacI PsiI PspOMI PspXI SacII
SanDI SfiI SgrAI SrfI SspI SwaI XhoI XmnI