Restriction map for
pF1KSDB1510
(Circular) (Six-base) MAPSORT of: pF1KSDB1510.seq Check: 5869 from: 1 to: 4880
pF1KSDB1510 4880 bp
With 182 enzymes: SgfI *
June 20, 2008 10:36 ..
AccI GT'mk_AC
Cuts at: 1004 1918 1004
Size: 914 3966
Acc65I G'GTAC_C
Cuts at: 715 1896 715
Size: 1181 3699
AclI AA'CG_TT
Cuts at: 4720 4720
Size: 4880
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1061 1308 1702 1711 2705 3137 3884 4152 1061
Size: 247 394 9 994 432 747 268 1789
Fragments arranged by size:
1789 994 747 432 394 268 247 9
AfeI AGC'GCT
Cuts at: 173 173
Size: 4880
AflIII A'CryG_T
Cuts at: 2232 3336 4313 2232
Size: 1104 977 2799
Fragments arranged by size:
2799 1104 977
AgeI A'CCGG_T
Cuts at: 3268 3268
Size: 4880
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 709 1327 709
Size: 618 4262
AlwNI CAG_nnn'CTG
Cuts at: 144 669 846 1164 1331 2185 3752 144
Size: 525 177 318 167 854 1567 1272
Fragments arranged by size:
1567 1272 854 525 318 177 167
ApaI G_GGCC'C
Cuts at: 232 409 759 999 232
Size: 177 350 240 4113
Fragments arranged by size:
4113 350 240 177
ApaLI G'TGCA_C
Cuts at: 722 3650 722
Size: 2928 1952
ApoI r'AATT_y
Cuts at: 1884 4233 4489 1884
Size: 2349 256 2275
Fragments arranged by size:
2349 2275 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4880
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4880
AvaI C'yCGr_G
Cuts at: 307 1549 1625 1900 307
Size: 1242 76 275 3287
Fragments arranged by size:
3287 1242 275 76
BamHI G'GATC_C
Cuts at: 1905 1905
Size: 4880
BanI G'GyrC_C
Cuts at: 715 1896 2310 2558 2593 715
Size: 1181 414 248 35 3002
Fragments arranged by size:
3002 1181 414 248 35
BanII G_rGCy'C
Cuts at: 232 308 314 409 750 759 999 1894 2924
Size: 76 6 95 341 9 240 895 1030
Cuts at: 2924 232
Size: 2188
Fragments arranged by size:
2188 1030 895 341 240 95 76 9
6
BbeI G_GCGC'C
Cuts at: 2314 2562 2314
Size: 248 4632
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 148 182 549 559 583 593 148
Size: 34 367 10 24 10 4435
Fragments arranged by size:
4435 367 34 24 10 10
BciVI GTATCCnnnnn_n'
Cuts at: 2778 3539 4880 2778
Size: 761 1341 2778
Fragments arranged by size:
2778 1341 761
BclI T'GATC_A
Cuts at: 2400 2400
Size: 4880
BglI GCCn_nnn'nGGC
Cuts at: 355 413 355
Size: 58 4822
BglII A'GATC_T
Cuts at: 2395 4432 2395
Size: 2037 2843
BlpI GC'TnA_GC
Cuts at: 1532 1992 1532
Size: 460 4420
Bme1580I G_kGCm'C
Cuts at: 232 409 726 759 999 1590 2505 2598 3654
Size: 177 317 33 240 591 915 93 1056
Cuts at: 3654 232
Size: 1458
Fragments arranged by size:
1458 1056 915 591 317 240 177 93
33
BmgBI CAC'GTC
Cuts at: 1569 1569
Size: 4880
BmrI ACTGGGnnnn_n'
Cuts at: 2092 2173 2508 4652 2092
Size: 81 335 2144 2320
Fragments arranged by size:
2320 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3247 3247
Size: 4880
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 604 691 795 1547 1579 1831 604
Size: 87 104 752 32 252 3653
Fragments arranged by size:
3653 752 252 104 87 32
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2056 2935 3427 3725 2056
Size: 879 492 298 3211
Fragments arranged by size:
3211 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 495 1202 1608 4584 495
Size: 707 406 2976 791
Fragments arranged by size:
2976 791 707 406
BsaAI yAC'GTr
Cuts at: 1338 1872 2233 1338
Size: 534 361 3985
Fragments arranged by size:
3985 534 361
BsaBI GATnn'nnATC
Cuts at: 6 2394 4431 6
Size: 2388 2037 455
Fragments arranged by size:
2388 2037 455
BsaHI Gr'CG_yC
Cuts at: 2311 2559 3261 4757 2311
Size: 248 702 1496 2434
Fragments arranged by size:
2434 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2069 2590 3268 3542 3689 4100 2069
Size: 521 678 274 147 411 2849
Fragments arranged by size:
2849 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 601 1708 1831 1834 601
Size: 1107 123 3 3647
Fragments arranged by size:
3647 1107 123 3
BseYI C'CCAG_C
Cuts at: 144 162 266 447 852 874 1126 2305 3063
Size: 18 104 181 405 22 252 1179 758
Cuts at: 3063 3640 4136 144
Size: 577 496 888
Fragments arranged by size:
1179 888 758 577 496 405 252 181
104 22 18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1526 1526
Size: 4880
BsiEI CG_ry'CG
Cuts at: 104 204 226 417 1733 2422 2468 3676 104
Size: 100 22 191 1316 689 46 1208 1308
Fragments arranged by size:
1316 1308 1208 689 191 100 46 22
BsiHKAI G_wGCw'C
Cuts at: 688 726 750 1894 2672 2862 3654 688
Size: 38 24 1144 778 190 792 1914
Fragments arranged by size:
1914 1144 792 778 190 38 24
BsmBI CGTCTCn'nnnn_
Cuts at: 328 555 328
Size: 227 4653
Bsp1286I G_dGCh'C
Cuts at: 232 308 314 409 688 726 750 759 999
Size: 76 6 95 279 38 24 9 240
Cuts at: 999 1590 1894 2505 2598 2672 2862 2924 3654
Size: 591 304 611 93 74 190 62 730
Cuts at: 3654 232
Size: 1458
Fragments arranged by size:
1458 730 611 591 304 279 240 190
95 93 76 74 62 38 24 9
6
BspEI T'CCGG_A
Cuts at: 4100 4100
Size: 4880
BspHI T'CATG_A
Cuts at: 4056 4056
Size: 4880
BspMI ACCTGCnnnn'nnnn_
Cuts at: 793 1548 1930 2144 2446 2827 793
Size: 755 382 214 302 381 2846
Fragments arranged by size:
2846 755 382 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3172 3226 4710 4875 50
Size: 3122 54 1484 165 55
Fragments arranged by size:
3122 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 655 2792 655
Size: 2137 2743
BsrFI r'CCGG_y
Cuts at: 479 2878 3059 3268 479
Size: 2399 181 209 2091
Fragments arranged by size:
2399 2091 209 181
BssSI C'ACGA_G
Cuts at: 558 3151 3509 558
Size: 2593 358 1929
Fragments arranged by size:
2593 1929 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2257 2257
Size: 4880
BstBI TT'CG_AA
Cuts at: 110 3241 110
Size: 3131 1749
BstXI CCAn_nnnn'nTGG
Cuts at: 1743 1743
Size: 4880
BstYI r'GATC_y
Cuts at: 1 506 1905 2372 2395 2730 2976 3988 4074
Size: 505 1399 467 23 335 246 1012 86
Cuts at: 4074 4086 4432 1
Size: 12 346 449
Fragments arranged by size:
1399 1012 505 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 125 150 249 1053 1382 125
Size: 25 99 804 329 3623
Fragments arranged by size:
3623 804 329 99 25
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1182 1434 2830 3011 3174 1182
Size: 252 1396 181 163 2888
Fragments arranged by size:
2888 1396 252 181 163
BtsI GCAGTG_nn'
Cuts at: 630 1412 1445 1543 1588 2123 630
Size: 782 33 98 45 535 3387
Fragments arranged by size:
3387 782 535 98 45 33
DraI TTT'AAA
Cuts at: 1879 1879
Size: 4880
DrdI GACnn_nn'nnGTC
Cuts at: 2586 3444 2586
Size: 858 4022
EaeI y'GGCC_r
Cuts at: 201 223 347 414 773 1056 1488 1730 1745
Size: 22 124 67 359 283 432 242 15
Cuts at: 1745 2465 2639 3030 3057 4402 201
Size: 720 174 391 27 1345 679
Fragments arranged by size:
1345 720 679 432 391 359 283 242
174 124 67 27 22 15
EagI C'GGCC_G
Cuts at: 201 223 414 1730 2465 201
Size: 22 191 1316 735 2616
Fragments arranged by size:
2616 1316 735 191 22
EarI CTCTTCn'nnn_
Cuts at: 978 2903 3113 978
Size: 1925 210 2745
Fragments arranged by size:
2745 1925 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 325 487 1364 3088 3398 3544 4690 325
Size: 162 877 1724 310 146 1146 515
Fragments arranged by size:
1724 1146 877 515 310 162 146
EcoICRI GAG'CTC
Cuts at: 748 1892 748
Size: 1144 3736
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 604 691 795 1061 1308 1547 1579 1702 1711
Size: 87 104 266 247 239 32 123 9
Cuts at: 1711 1831 2705 3137 3884 4152 604
Size: 120 874 432 747 268 1332
Fragments arranged by size:
1332 874 747 432 268 266 247 239
123 120 104 87 32 9
EcoNI CCTnn'n_nnAGG
Cuts at: 457 457
Size: 4880
EcoO109I rG'GnC_Cy
Cuts at: 406 541 755 756 916 995 996 2019 406
Size: 135 214 1 160 79 1 1023 3267
Fragments arranged by size:
3267 1023 214 160 135 79 1 1
EcoRI G'AATT_C
Cuts at: 1884 1884
Size: 4880
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1812 2899 1812
Size: 1087 3793
FspI TGC'GCA
Cuts at: 2661 2661
Size: 4880
HaeII r_GCGC'y
Cuts at: 145 175 2314 2562 3584 4563 145
Size: 30 2139 248 1022 979 462
Fragments arranged by size:
2139 1022 979 462 248 30
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1201 1233 2722 2752 2754 2784 1201
Size: 32 1489 30 2 30 3297
Fragments arranged by size:
3297 1489 32 30 30 2
HincII GTy'rAC
Cuts at: 1005 1919 1005
Size: 914 3966
KasI G'GCGC_C
Cuts at: 2310 2558 2310
Size: 248 4632
KpnI G_GTAC'C
Cuts at: 719 1900 719
Size: 1181 3699
MluI A'CGCG_T
Cuts at: 4313 4313
Size: 4880
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 533 1244 1746 3551 3735 533
Size: 711 502 1805 184 1678
Fragments arranged by size:
1805 1678 711 502 184
MscI TGG'CCA
Cuts at: 775 1747 2641 775
Size: 972 894 3014
Fragments arranged by size:
3014 972 894
MslI CAynn'nnrTG
Cuts at: 130 781 1231 2869 2996 130
Size: 651 450 1638 127 2014
Fragments arranged by size:
2014 1638 651 450 127
MspA1I CmG'CkG
Cuts at: 152 162 251 372 984 1161 1331 1991 2305
Size: 10 89 121 612 177 170 660 314
Cuts at: 2305 2665 3678 3923 152
Size: 360 1013 245 1109
Fragments arranged by size:
1109 1013 660 612 360 314 245 177
170 121 89 10
NaeI GCC'GGC
Cuts at: 481 3061 481
Size: 2580 2300
NarI GG'CG_CC
Cuts at: 2311 2559 2311
Size: 248 4632
NcoI C'CATG_G
Cuts at: 125 1053 125
Size: 928 3952
NgoMIV G'CCGG_C
Cuts at: 479 3059 479
Size: 2580 2300
NspI r_CATG'y
Cuts at: 1933 2433 3340 1933
Size: 500 907 3473
Fragments arranged by size:
3473 907 500
PasI CC'CwG_GG
Cuts at: 1400 1400
Size: 4880
PciI A'CATG_T
Cuts at: 3336 3336
Size: 4880
PflMI CCAn_nnn'nTGG
Cuts at: 345 669 846 345
Size: 324 177 4379
Fragments arranged by size:
4379 324 177
PmeI GTTT'AAAC
Cuts at: 1879 1879
Size: 4880
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4019 4051 4019
Size: 32 4848
PpuMI rG'GwC_Cy
Cuts at: 916 916
Size: 4880
PspOMI G'GGCC_C
Cuts at: 228 405 755 995 228
Size: 177 350 240 4113
Fragments arranged by size:
4113 350 240 177
PstI C_TGCA'G
Cuts at: 161 625 1160 1316 1927 2612 161
Size: 464 535 156 611 685 2429
Fragments arranged by size:
2429 685 611 535 464 156
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4880
PvuII CAG'CTG
Cuts at: 162 1161 1331 2305 2665 162
Size: 999 170 974 360 2377
Fragments arranged by size:
2377 999 974 360 170
RsrII CG'GwC_CG
Cuts at: 3075 3075
Size: 4880
SacI G_AGCT'C
Cuts at: 750 1894 750
Size: 1144 3736
SacII CC_GC'GG
Cuts at: 153 252 153
Size: 99 4781
SalI G'TCGA_C
Cuts at: 1003 1917 1003
Size: 914 3966
SapI GCTCTTCn'nnn_
Cuts at: 2903 3113 2903
Size: 210 4670
SbfI CC_TGCA'GG
Cuts at: 1927 1927
Size: 4880
ScaI AGT'ACT
Cuts at: 4397 4397
Size: 4880
SfcI C'TryA_G
Cuts at: 33 157 621 1156 1312 1416 1923 2608 3601
Size: 124 464 535 156 104 507 685 993
Cuts at: 3601 3792 33
Size: 191 1121
Fragments arranged by size:
1121 993 685 535 507 464 191 156
124 104
SfiI GGCCn_nnn'nGGCC
Cuts at: 413 413
Size: 4880
SfoI GGC'GCC
Cuts at: 2312 2560 2312
Size: 248 4632
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4880
SmaI CCC'GGG
Cuts at: 1902 1902
Size: 4880
SmlI C'TyrA_G
Cuts at: 2035 2950 3442 3704 2035
Size: 915 492 262 3211
Fragments arranged by size:
3211 915 492 262
SnaBI TAC'GTA
Cuts at: 1872 1872
Size: 4880
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4880
SphI G_CATG'C
Cuts at: 1933 2433 1933
Size: 500 4380
StuI AGG'CCT
Cuts at: 1046 1046
Size: 4880
StyI C'CwwG_G
Cuts at: 125 770 869 1053 2014 2368 125
Size: 645 99 184 961 354 2637
Fragments arranged by size:
2637 961 645 354 184 99
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1140 2463 2596 3055 3264 1140
Size: 1323 133 459 209 2756
Fragments arranged by size:
2756 1323 459 209 133
TatI w'GTAC_w
Cuts at: 4395 4395
Size: 4880
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1374 1406 4397 4429 1374
Size: 32 2991 32 1825
Fragments arranged by size:
2991 1825 32 32
Tth111I GACn'n_nGTC
Cuts at: 2677 2677
Size: 4880
XbaI T'CTAG_A
Cuts at: 1911 1911
Size: 4880
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2375 2375
Size: 4880
XmaI C'CCGG_G
Cuts at: 1900 1900
Size: 4880
XmnI GAAnn'nnTTC
Cuts at: 1670 1670
Size: 4880
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PasI PciI PflMI
PmeI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI ScaI SfcI SfiI
SfoI SgfI SmaI SmlI SnaBI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AloI AscI AvrII
BaeI BbsI BbvCI BfrBI BmtI Bpu10I BsaXI BsiWI
BsmI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI
DraIII EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PfoI PmlI PshAI
PsiI PspXI PsrI SanDI SexAI SgrAI SrfI SspI
SwaI XhoI ZraI