Restriction map for
pF1KSDB2506
(Circular) (Six-base) MAPSORT of: pF1KSDB2506.seq Check: 5063 from: 1 to: 4346
pF1KSDB2506 4346 bp
With 182 enzymes: SgfI *
June 20, 2008 10:36 ..
AccI GT'mk_AC
Cuts at: 1384 1384
Size: 4346
Acc65I G'GTAC_C
Cuts at: 1362 1362
Size: 4346
AclI AA'CG_TT
Cuts at: 4186 4186
Size: 4346
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2171 2603 3350 3618 2171
Size: 432 747 268 2899
Fragments arranged by size:
2899 747 432 268
AfeI AGC'GCT
Cuts at: 1230 1230
Size: 4346
AflIII A'CryG_T
Cuts at: 260 1052 1247 1698 2802 3779 260
Size: 792 195 451 1104 977 827
Fragments arranged by size:
1104 977 827 792 451 195
AgeI A'CCGG_T
Cuts at: 2734 2734
Size: 4346
AleI CACnn'nnGTG
Cuts at: 199 199
Size: 4346
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 935 1030 935
Size: 95 4251
AlwNI CAG_nnn'CTG
Cuts at: 965 1651 3218 965
Size: 686 1567 2093
Fragments arranged by size:
2093 1567 686
ApaI G_GGCC'C
Cuts at: 595 595
Size: 4346
ApaLI G'TGCA_C
Cuts at: 3116 3116
Size: 4346
ApoI r'AATT_y
Cuts at: 1350 3699 3955 1350
Size: 2349 256 1741
Fragments arranged by size:
2349 1741 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4346
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4346
AvaI C'yCGr_G
Cuts at: 227 1366 227
Size: 1139 3207
BamHI G'GATC_C
Cuts at: 1371 1371
Size: 4346
BanI G'GyrC_C
Cuts at: 322 1362 1776 2024 2059 322
Size: 1040 414 248 35 2609
Fragments arranged by size:
2609 1040 414 248 35
BanII G_rGCy'C
Cuts at: 159 400 595 716 1360 2390 159
Size: 241 195 121 644 1030 2115
Fragments arranged by size:
2115 1030 644 241 195 121
BbeI G_GCGC'C
Cuts at: 326 1780 2028 326
Size: 1454 248 2644
Fragments arranged by size:
2644 1454 248
BbsI GAAGACnn'nnnn_
Cuts at: 963 1329 963
Size: 366 3980
BciVI GTATCCnnnnn_n'
Cuts at: 2244 3005 4346 2244
Size: 761 1341 2244
Fragments arranged by size:
2244 1341 761
BclI T'GATC_A
Cuts at: 191 329 1866 191
Size: 138 1537 2671
Fragments arranged by size:
2671 1537 138
BglI GCCn_nnn'nGGC
Cuts at: 404 599 404
Size: 195 4151
BglII A'GATC_T
Cuts at: 1861 3898 1861
Size: 2037 2309
BlpI GC'TnA_GC
Cuts at: 547 1458 547
Size: 911 3435
Bme1580I G_kGCm'C
Cuts at: 595 1971 2064 3120 595
Size: 1376 93 1056 1821
Fragments arranged by size:
1821 1376 1056 93
BmgBI CAC'GTC
Cuts at: 1055 1095 1055
Size: 40 4306
BmrI ACTGGGnnnn_n'
Cuts at: 1196 1558 1639 1974 4118 1196
Size: 362 81 335 2144 1424
Fragments arranged by size:
2144 1424 362 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2713 2713
Size: 4346
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1522 2401 2893 3191 1522
Size: 879 492 298 2677
Fragments arranged by size:
2677 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 475 4050 475
Size: 3575 771
BsaAI yAC'GTr
Cuts at: 261 1338 1699 261
Size: 1077 361 2908
Fragments arranged by size:
2908 1077 361
BsaBI GATnn'nnATC
Cuts at: 6 349 1860 3897 6
Size: 343 1511 2037 455
Fragments arranged by size:
2037 1511 455 343
BsaHI Gr'CG_yC
Cuts at: 281 323 515 1777 2025 2727 4223 281
Size: 42 192 1262 248 702 1496 404
Fragments arranged by size:
1496 1262 702 404 248 192 42
BsaWI w'CCGG_w
Cuts at: 1535 2056 2734 3008 3155 3566 1535
Size: 521 678 274 147 411 2315
Fragments arranged by size:
2315 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 238 823 1102 238
Size: 585 279 3482
Fragments arranged by size:
3482 585 279
BseYI C'CCAG_C
Cuts at: 965 1225 1771 2529 3106 3602 965
Size: 260 546 758 577 496 1709
Fragments arranged by size:
1709 758 577 546 496 260
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 990 1117 990
Size: 127 4219
BsiEI CG_ry'CG
Cuts at: 104 1888 1934 3142 104
Size: 1784 46 1208 1308
Fragments arranged by size:
1784 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 159 170 674 860 1280 1360 2138 2328 3120
Size: 11 504 186 420 80 778 190 792
Cuts at: 3120 159
Size: 1385
Fragments arranged by size:
1385 792 778 504 420 190 186 80
11
BsmBI CGTCTCn'nnnn_
Cuts at: 1069 1069
Size: 4346
Bsp1286I G_dGCh'C
Cuts at: 159 170 400 595 674 716 860 1280 1360
Size: 11 230 195 79 42 144 420 80
Cuts at: 1360 1971 2064 2138 2328 2390 3120 159
Size: 611 93 74 190 62 730 1385
Fragments arranged by size:
1385 730 611 420 230 195 190 144
93 80 79 74 62 42 11
BspEI T'CCGG_A
Cuts at: 3566 3566
Size: 4346
BspHI T'CATG_A
Cuts at: 3522 3522
Size: 4346
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1396 1610 1912 2293 1396
Size: 214 302 381 3449
Fragments arranged by size:
3449 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2638 2692 4176 4341 50
Size: 2588 54 1484 165 55
Fragments arranged by size:
2588 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 903 2258 903
Size: 1355 2991
BsrFI r'CCGG_y
Cuts at: 2344 2525 2734 2344
Size: 181 209 3956
Fragments arranged by size:
3956 209 181
BssSI C'ACGA_G
Cuts at: 2617 2975 2617
Size: 358 3988
BstAPI GCAn_nnn'nTGC
Cuts at: 1723 1723
Size: 4346
BstBI TT'CG_AA
Cuts at: 110 2707 110
Size: 2597 1749
BstEII G'GTnAC_C
Cuts at: 762 762
Size: 4346
BstXI CCAn_nnnn'nTGG
Cuts at: 132 951 132
Size: 819 3527
BstYI r'GATC_y
Cuts at: 1 998 1371 1838 1861 2196 2442 3454 3540
Size: 997 373 467 23 335 246 1012 86
Cuts at: 3540 3552 3898 1
Size: 12 346 449
Fragments arranged by size:
1012 997 467 449 373 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 596 716 1105 1136 596
Size: 120 389 31 3806
Fragments arranged by size:
3806 389 120 31
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 636 2296 2477 2640 636
Size: 1660 181 163 2342
Fragments arranged by size:
2342 1660 181 163
BtsI GCAGTG_nn'
Cuts at: 334 1002 1589 334
Size: 668 587 3091
Fragments arranged by size:
3091 668 587
DraI TTT'AAA
Cuts at: 1345 1345
Size: 4346
DraIII CAC_nnn'GTG
Cuts at: 165 791 165
Size: 626 3720
DrdI GACnn_nn'nnGTC
Cuts at: 2052 2910 2052
Size: 858 3488
EaeI y'GGCC_r
Cuts at: 311 941 956 1268 1931 2105 2496 2523 3868
Size: 630 15 312 663 174 391 27 1345
Cuts at: 3868 311
Size: 789
Fragments arranged by size:
1345 789 663 630 391 312 174 27
15
EagI C'GGCC_G
Cuts at: 1931 1931
Size: 4346
EarI CTCTTCn'nnn_
Cuts at: 190 388 787 895 2369 2579 190
Size: 198 399 108 1474 210 1957
Fragments arranged by size:
1957 1474 399 210 198 108
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 487 1295 2554 2864 3010 4156 487
Size: 808 1259 310 146 1146 677
Fragments arranged by size:
1259 1146 808 677 310 146
EcoICRI GAG'CTC
Cuts at: 157 1358 157
Size: 1201 3145
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 2171 2603 3350 3618 2171
Size: 432 747 268 2899
Fragments arranged by size:
2899 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 431 592 824 1485 431
Size: 161 232 661 3292
Fragments arranged by size:
3292 661 232 161
EcoRI G'AATT_C
Cuts at: 1350 1350
Size: 4346
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2365 2365
Size: 4346
FspI TGC'GCA
Cuts at: 2127 2127
Size: 4346
HaeII r_GCGC'y
Cuts at: 326 625 1232 1780 2028 3050 4029 326
Size: 299 607 548 248 1022 979 643
Fragments arranged by size:
1022 979 643 607 548 299 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 141 173 2188 2218 2220 2250 141
Size: 32 2015 30 2 30 2237
Fragments arranged by size:
2237 2015 32 30 30 2
HincII GTy'rAC
Cuts at: 1385 1385
Size: 4346
KasI G'GCGC_C
Cuts at: 322 1776 2024 322
Size: 1454 248 2644
Fragments arranged by size:
2644 1454 248
KpnI G_GTAC'C
Cuts at: 1366 1366
Size: 4346
MluI A'CGCG_T
Cuts at: 3779 3779
Size: 4346
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3017 3201 3017
Size: 184 4162
MscI TGG'CCA
Cuts at: 943 2107 943
Size: 1164 3182
MslI CAynn'nnrTG
Cuts at: 130 199 298 655 865 2335 2462 130
Size: 69 99 357 210 1470 127 2014
Fragments arranged by size:
2014 1470 357 210 127 99 69
MspA1I CmG'CkG
Cuts at: 598 609 962 1101 1107 1457 1771 2131 3144
Size: 11 353 139 6 350 314 360 1013
Cuts at: 3144 3389 598
Size: 245 1555
Fragments arranged by size:
1555 1013 360 353 350 314 245 139
11 6
NaeI GCC'GGC
Cuts at: 2527 2527
Size: 4346
NarI GG'CG_CC
Cuts at: 323 1777 2025 323
Size: 1454 248 2644
Fragments arranged by size:
2644 1454 248
NcoI C'CATG_G
Cuts at: 716 1136 716
Size: 420 3926
NgoMIV G'CCGG_C
Cuts at: 2525 2525
Size: 4346
NspI r_CATG'y
Cuts at: 1399 1899 2806 1399
Size: 500 907 2939
Fragments arranged by size:
2939 907 500
PasI CC'CwG_GG
Cuts at: 435 768 435
Size: 333 4013
PciI A'CATG_T
Cuts at: 2802 2802
Size: 4346
PflMI CCAn_nnn'nTGG
Cuts at: 965 965
Size: 4346
PfoI T'CCnGG_A
Cuts at: 470 470
Size: 4346
PmeI GTTT'AAAC
Cuts at: 1345 1345
Size: 4346
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3485 3517 3485
Size: 32 4314
PpuMI rG'GwC_Cy
Cuts at: 431 431
Size: 4346
PspOMI G'GGCC_C
Cuts at: 591 591
Size: 4346
PstI C_TGCA'G
Cuts at: 911 1028 1393 2078 911
Size: 117 365 685 3179
Fragments arranged by size:
3179 685 365 117
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4346
PvuII CAG'CTG
Cuts at: 609 1101 1771 2131 609
Size: 492 670 360 2824
Fragments arranged by size:
2824 670 492 360
RsrII CG'GwC_CG
Cuts at: 2541 2541
Size: 4346
SacI G_AGCT'C
Cuts at: 159 1360 159
Size: 1201 3145
SacII CC_GC'GG
Cuts at: 599 1108 599
Size: 509 3837
SalI G'TCGA_C
Cuts at: 1383 1383
Size: 4346
SanDI GG'GwC_CC
Cuts at: 431 431
Size: 4346
SapI GCTCTTCn'nnn_
Cuts at: 190 388 2369 2579 190
Size: 198 1981 210 1957
Fragments arranged by size:
1981 1957 210 198
SbfI CC_TGCA'GG
Cuts at: 1028 1393 1028
Size: 365 3981
ScaI AGT'ACT
Cuts at: 3863 3863
Size: 4346
SexAI A'CCwGG_T
Cuts at: 161 161
Size: 4346
SfcI C'TryA_G
Cuts at: 33 907 1024 1389 2074 3067 3258 33
Size: 874 117 365 685 993 191 1121
Fragments arranged by size:
1121 993 874 685 365 191 117
SfoI GGC'GCC
Cuts at: 324 1778 2026 324
Size: 1454 248 2644
Fragments arranged by size:
2644 1454 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4346
SmaI CCC'GGG
Cuts at: 1368 1368
Size: 4346
SmlI C'TyrA_G
Cuts at: 1501 2416 2908 3170 1501
Size: 915 492 262 2677
Fragments arranged by size:
2677 915 492 262
SnaBI TAC'GTA
Cuts at: 1338 1338
Size: 4346
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4346
SphI G_CATG'C
Cuts at: 1399 1899 1399
Size: 500 3846
StuI AGG'CCT
Cuts at: 1129 1129
Size: 4346
StyI C'CwwG_G
Cuts at: 716 938 1136 1480 1834 716
Size: 222 198 344 354 3228
Fragments arranged by size:
3228 354 344 222 198
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 855 1929 2062 2521 2730 855
Size: 1074 133 459 209 2471
Fragments arranged by size:
2471 1074 459 209 133
TatI w'GTAC_w
Cuts at: 3861 3861
Size: 4346
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3863 3895 3863
Size: 32 4314
Tth111I GACn'n_nGTC
Cuts at: 2143 2143
Size: 4346
XbaI T'CTAG_A
Cuts at: 1377 1377
Size: 4346
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1185 1209 1841 1185
Size: 24 632 3690
Fragments arranged by size:
3690 632 24
XmaI C'CCGG_G
Cuts at: 1366 1366
Size: 4346
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AleI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PspOMI PstI PvuI
PvuII RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SnaBI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AloI AscI AvrII BaeI
BbvCI BcgI BfrBI BmtI BpmI Bpu10I BsaXI BsiWI
BsmI BsrGI BssHII BstZ17I Bsu36I ClaI CspCI EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PmlI PshAI PsiI PspXI
PsrI SfiI SgrAI SrfI SspI SwaI XhoI XmnI
ZraI