Restriction map for
pF1KSDF0002
(Circular) (Six-base) MAPSORT of: pF1KSDF0002.seq Check: 2603 from: 1 to: 7615
pF1KSDF0002 7615 bp
With 182 enzymes: SgfI *
June 20, 2008 10:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2423 2423
Size: 7615
AccI GT'mk_AC
Cuts at: 1646 2345 4651 1646
Size: 699 2306 4610
Fragments arranged by size:
4610 2306 699
Acc65I G'GTAC_C
Cuts at: 1425 4629 1425
Size: 3204 4411
AclI AA'CG_TT
Cuts at: 7455 7455
Size: 7615
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 788 1018 1168 2884 4213 5438 5870 6619 6887
Size: 230 150 1716 1329 1225 432 749 268
Cuts at: 6887 788
Size: 1516
Fragments arranged by size:
1716 1516 1329 1225 749 432 268 230
150
AfeI AGC'GCT
Cuts at: 3618 3618
Size: 7615
AflIII A'CryG_T
Cuts at: 2738 4965 6071 7048 2738
Size: 2227 1106 977 3305
Fragments arranged by size:
3305 2227 1106 977
AgeI A'CCGG_T
Cuts at: 1665 6003 1665
Size: 4338 3277
AhdI GACnn_n'nnGTC
Cuts at: 1615 1615
Size: 7615
AleI CACnn'nnGTG
Cuts at: 942 1732 4208 942
Size: 790 2476 4349
Fragments arranged by size:
4349 2476 790
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 622 680 1508 622
Size: 58 828 6729
Fragments arranged by size:
6729 828 58
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2132 2164 2132
Size: 32 7583
AlwNI CAG_nnn'CTG
Cuts at: 266 700 1566 3576 3825 4191 4554 4557 4918
Size: 434 866 2010 249 366 363 3 361
Cuts at: 4918 6487 266
Size: 1569 1394
Fragments arranged by size:
2010 1569 1394 866 434 366 363 361
249 3
ApaI G_GGCC'C
Cuts at: 629 1034 1241 629
Size: 405 207 7003
Fragments arranged by size:
7003 405 207
ApaLI G'TGCA_C
Cuts at: 6385 6385
Size: 7615
ApoI r'AATT_y
Cuts at: 3057 4617 6968 7224 3057
Size: 1560 2351 256 3448
Fragments arranged by size:
3448 2351 1560 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 7615
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7615
AvaI C'yCGr_G
Cuts at: 282 515 742 1574 1595 3720 4633 282
Size: 233 227 832 21 2125 913 3264
Fragments arranged by size:
3264 2125 913 832 233 227 21
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2235 2268 2235
Size: 33 7582
BamHI G'GATC_C
Cuts at: 1835 2147 3194 4638 1835
Size: 312 1047 1444 4812
Fragments arranged by size:
4812 1444 1047 312
BanI G'GyrC_C
Cuts at: 277 323 813 1425 1712 2525 2722 3100 3156
Size: 46 490 612 287 813 197 378 56
Cuts at: 3156 3404 3542 3835 4629 5043 5291 5326 277
Size: 248 138 293 794 414 248 35 2566
Fragments arranged by size:
2566 813 794 612 490 414 378 293
287 248 248 197 138 56 46 35
BanII G_rGCy'C
Cuts at: 629 726 734 848 1034 1241 1575 1596 2047
Size: 97 8 114 186 207 334 21 451
Cuts at: 2047 2075 2363 2924 3293 3310 4022 4115 4311
Size: 28 288 561 369 17 712 93 196
Cuts at: 4311 4627 5657 629
Size: 316 1030 2587
Fragments arranged by size:
2587 1030 712 561 451 369 334 316
288 207 196 186 114 97 93 28
21 17 8
BbeI G_GCGC'C
Cuts at: 327 2529 2726 3408 5047 5295 327
Size: 2202 197 682 1639 248 2647
Fragments arranged by size:
2647 2202 1639 682 248 197
BbsI GAAGACnn'nnnn_
Cuts at: 351 903 2194 3977 4375 351
Size: 552 1291 1783 398 3591
Fragments arranged by size:
3591 1783 1291 552 398
BciVI GTATCCnnnnn_n'
Cuts at: 2263 5511 6274 7615 2263
Size: 3248 763 1341 2263
Fragments arranged by size:
3248 2263 1341 763
BclI T'GATC_A
Cuts at: 1796 5133 1796
Size: 3337 4278
BglI GCCn_nnn'nGGC
Cuts at: 596 811 832 1123 1591 3274 3487 3971 596
Size: 215 21 291 468 1683 213 484 4240
Fragments arranged by size:
4240 1683 484 468 291 215 213 21
BglII A'GATC_T
Cuts at: 5128 7167 5128
Size: 2039 5576
BlpI GC'TnA_GC
Cuts at: 226 2443 4725 226
Size: 2217 2282 3116
Fragments arranged by size:
3116 2282 2217
Bme1580I G_kGCm'C
Cuts at: 280 629 939 1034 1241 1715 2322 3838 5238
Size: 349 310 95 207 474 607 1516 1400
Cuts at: 5238 5331 6389 280
Size: 93 1058 1506
Fragments arranged by size:
1516 1506 1400 1058 607 474 349 310
207 95 93
BmgBI CAC'GTC
Cuts at: 3876 3876
Size: 7615
BmrI ACTGGGnnnn_n'
Cuts at: 694 1265 1576 1945 2622 2965 4016 4083 4825
Size: 571 311 369 677 343 1051 67 742
Cuts at: 4825 4906 5241 7387 694
Size: 81 335 2146 922
Fragments arranged by size:
2146 1051 922 742 677 571 369 343
335 311 81 67
BmtI G_CTAG'C
Cuts at: 1117 1117
Size: 7615
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2631 2895 4289 4524 5980 2631
Size: 264 1394 235 1456 4266
Fragments arranged by size:
4266 1456 1394 264 235
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 542 1831 1987 2087 2285 2509 2686 3962 4087
Size: 1289 156 100 198 224 177 1276 125
Cuts at: 4087 4369 542
Size: 282 3788
Fragments arranged by size:
3788 1289 1276 282 224 198 177 156
125 100
Bpu10I CC'TnA_GC
Cuts at: 725 2302 2781 725
Size: 1577 479 5559
Fragments arranged by size:
5559 1577 479
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3547 4789 5668 6162 6460 3547
Size: 1242 879 494 298 4702
Fragments arranged by size:
4702 1242 879 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 1408 1810 1963 2261 7319 1408
Size: 402 153 298 5058 1704
Fragments arranged by size:
5058 1704 402 298 153
BsaAI yAC'GTr
Cuts at: 2739 3366 4605 4966 2739
Size: 627 1239 361 5388
Fragments arranged by size:
5388 1239 627 361
BsaBI GATnn'nnATC
Cuts at: 6 5127 7166 6
Size: 5121 2039 455
Fragments arranged by size:
5121 2039 455
BsaHI Gr'CG_yC
Cuts at: 324 2526 2723 3405 5044 5292 5996 7492 324
Size: 2202 197 682 1639 248 704 1496 447
Fragments arranged by size:
2202 1639 1496 704 682 447 248 197
BsaWI w'CCGG_w
Cuts at: 1665 1832 4802 5323 6003 6277 6424 6835 1665
Size: 167 2970 521 680 274 147 411 2445
Fragments arranged by size:
2970 2445 680 521 411 274 167 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1562 1592 3193 3223 3370 3400 3411 3441 1562
Size: 30 1601 30 147 30 11 30 5736
Fragments arranged by size:
5736 1601 147 30 30 30 30 11
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 238 300 2359 2683 2686 2932 2997 3201 3378
Size: 62 2059 324 3 246 65 204 177
Cuts at: 3378 3520 3523 4303 238
Size: 142 3 780 3550
Fragments arranged by size:
3550 2059 780 324 246 204 177 142
65 62 3 3
BseYI C'CCAG_C
Cuts at: 143 215 1131 1233 1240 1309 1602 2907 3285
Size: 72 916 102 7 69 293 1305 378
Cuts at: 3285 3303 3831 4076 4248 5038 5796 6375 6871
Size: 18 528 245 172 790 758 579 496
Cuts at: 6871 143
Size: 887
Fragments arranged by size:
1305 916 887 790 758 579 528 496
378 293 245 172 102 72 69 18
7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 650 2404 2451 3051 3388 3661 3955 650
Size: 1754 47 600 337 273 294 4310
Fragments arranged by size:
4310 1754 600 337 294 273 47
BsiEI CG_ry'CG
Cuts at: 104 5155 5201 6411 104
Size: 5051 46 1210 1308
Fragments arranged by size:
5051 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 227 260 848 2047 2075 2363 2683 3089 3476
Size: 33 588 1199 28 288 320 406 387
Cuts at: 3476 4004 4627 5405 5595 6389 227
Size: 528 623 778 190 794 1453
Fragments arranged by size:
1453 1199 794 778 623 588 528 406
387 320 288 190 33 28
BsmI GAATG_Cn'
Cuts at: 1529 1766 3912 1529
Size: 237 2146 5232
Fragments arranged by size:
5232 2146 237
Bsp1286I G_dGCh'C
Cuts at: 227 260 280 629 726 734 848 939 1034
Size: 33 20 349 97 8 114 91 95
Cuts at: 1034 1241 1575 1596 1715 2047 2075 2322 2363
Size: 207 334 21 119 332 28 247 41
Cuts at: 2363 2683 2924 3089 3293 3310 3476 3838 4004
Size: 320 241 165 204 17 166 362 166
Cuts at: 4004 4022 4115 4311 4627 5238 5331 5405 5595
Size: 18 93 196 316 611 93 74 190
Cuts at: 5595 5657 6389 227
Size: 62 732 1453
Fragments arranged by size:
1453 732 611 362 349 334 332 320
316 247 241 207 204 196 190 166
166 165 119 114 97 95 93 93
91 74 62 41 33 28 21 20
18 17 8
BspEI T'CCGG_A
Cuts at: 6835 6835
Size: 7615
BspHI T'CATG_A
Cuts at: 6791 6791
Size: 7615
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1368 1373 2423 3524 3806 4663 4877 5179 5560
Size: 5 1050 1101 282 857 214 302 381
Cuts at: 5560 1368
Size: 3423
Fragments arranged by size:
3423 1101 1050 857 381 302 282 214
5
BsrBI CCG'CTC
Cuts at: 50 174 3774 5905 5959 7445 7610 50
Size: 124 3600 2131 54 1486 165 55
Fragments arranged by size:
3600 2131 1486 165 124 55 54
BsrDI GCAATG_nn'
Cuts at: 585 3044 4430 5525 585
Size: 2459 1386 1095 2675
Fragments arranged by size:
2675 2459 1386 1095
BsrFI r'CCGG_y
Cuts at: 1117 1665 3838 3890 5611 5792 6003 1117
Size: 548 2173 52 1721 181 211 2729
Fragments arranged by size:
2729 2173 1721 548 211 181 52
BsrGI T'GTAC_A
Cuts at: 2207 2207
Size: 7615
BssSI C'ACGA_G
Cuts at: 710 905 2336 5884 6244 710
Size: 195 1431 3548 360 2081
Fragments arranged by size:
3548 2081 1431 360 195
BstAPI GCAn_nnn'nTGC
Cuts at: 4557 4990 4557
Size: 433 7182
BstBI TT'CG_AA
Cuts at: 110 110
Size: 7615
BstXI CCAn_nnnn'nTGG
Cuts at: 4065 4065
Size: 7615
BstYI r'GATC_y
Cuts at: 1 1835 1890 2147 2852 3194 4084 4328 4638
Size: 1834 55 257 705 342 890 244 310
Cuts at: 4638 5105 5128 5463 5709 6723 6809 6821 7167
Size: 467 23 335 246 1014 86 12 346
Cuts at: 7167 1
Size: 449
Fragments arranged by size:
1834 1014 890 705 467 449 346 342
335 310 257 246 244 86 55 23
12
BstZ17I GTA'TAC
Cuts at: 1647 1647
Size: 7615
Bsu36I CC'TnA_GG
Cuts at: 758 857 758
Size: 99 7516
BtgI C'CryG_G
Cuts at: 125 629 828 1089 2726 2762 3185 4226 125
Size: 504 199 261 1637 36 423 1041 3514
Fragments arranged by size:
3514 1637 1041 504 423 261 199 36
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2046 3112 3615 5563 5744 5907 2046
Size: 1066 503 1948 181 163 3754
Fragments arranged by size:
3754 1948 1066 503 181 163
BtsI GCAGTG_nn'
Cuts at: 204 1414 1721 2105 2980 3089 3577 3614 3823
Size: 1210 307 384 875 109 488 37 209
Cuts at: 3823 4204 4856 204
Size: 381 652 2963
Fragments arranged by size:
2963 1210 875 652 488 384 381 307
209 109 37
ClaI AT'CG_AT
Cuts at: 4340 4340
Size: 7615
DraI TTT'AAA
Cuts at: 4612 4612
Size: 7615
DrdI GACnn_nn'nnGTC
Cuts at: 5319 6179 5319
Size: 860 6755
EaeI y'GGCC_r
Cuts at: 169 507 1349 1460 2129 2384 2405 3266 3296
Size: 338 842 111 669 255 21 861 30
Cuts at: 3296 3446 3892 4301 4514 5198 5372 5763 5790
Size: 150 446 409 213 684 174 391 27
Cuts at: 5790 7137 169
Size: 1347 647
Fragments arranged by size:
1347 861 842 684 669 647 446 409
391 338 255 213 174 150 111 30
27 21
EagI C'GGCC_G
Cuts at: 5198 5198
Size: 7615
EarI CTCTTCn'nnn_
Cuts at: 1061 2035 2797 2929 3776 4009 5636 5846 1061
Size: 974 762 132 847 233 1627 210 2830
Fragments arranged by size:
2830 1627 974 847 762 233 210 132
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 5821 6133 6279 7425 5821
Size: 312 146 1146 6011
Fragments arranged by size:
6011 1146 312 146
EcoICRI GAG'CTC
Cuts at: 846 2045 2073 2361 4625 846
Size: 1199 28 288 2264 3836
Fragments arranged by size:
3836 2264 1199 288 28
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 542 788 1018 1168 1831 1987 2087 2285 2509
Size: 246 230 150 663 156 100 198 224
Cuts at: 2509 2686 2884 3962 4087 4213 4369 5438 5870
Size: 177 198 1078 125 126 156 1069 432
Cuts at: 5870 6619 6887 542
Size: 749 268 1270
Fragments arranged by size:
1270 1078 1069 749 663 432 268 246
230 224 198 198 177 156 156 150
126 125 100
EcoNI CCTnn'n_nnAGG
Cuts at: 742 1066 2698 3802 4165 4210 742
Size: 324 1632 1104 363 45 4147
Fragments arranged by size:
4147 1632 1104 363 324 45
EcoO109I rG'GnC_Cy
Cuts at: 412 520 625 833 950 962 1030 1031 1237
Size: 108 105 208 117 12 68 1 206
Cuts at: 1237 1263 1332 1947 3383 3654 4158 4175 4188
Size: 26 69 615 1436 271 504 17 13
Cuts at: 4188 4597 4752 412
Size: 409 155 3275
Fragments arranged by size:
3275 1436 615 504 409 271 208 206
155 117 108 105 69 68 26 17
13 12 1
EcoRI G'AATT_C
Cuts at: 4617 4617
Size: 7615
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 5632 5632
Size: 7615
FspI TGC'GCA
Cuts at: 4525 5394 4525
Size: 869 6746
HaeII r_GCGC'y
Cuts at: 233 327 2529 2726 3375 3408 3620 5047 5295
Size: 94 2202 197 649 33 212 1427 248
Cuts at: 5295 6319 7298 233
Size: 1024 979 550
Fragments arranged by size:
2202 1427 1024 979 649 550 248 212
197 94 33
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 311 343 1601 1633 1788 1820 2159 2191 5455
Size: 32 1258 32 155 32 339 32 3264
Cuts at: 5455 5485 5487 5517 311
Size: 30 2 30 2409
Fragments arranged by size:
3264 2409 1258 339 155 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1434 4652 1434
Size: 3218 4397
HindIII A'AGCT_T
Cuts at: 1504 2747 1504
Size: 1243 6372
KasI G'GCGC_C
Cuts at: 323 2525 2722 3404 5043 5291 323
Size: 2202 197 682 1639 248 2647
Fragments arranged by size:
2647 2202 1639 682 248 197
KpnI G_GTAC'C
Cuts at: 1429 4633 1429
Size: 3204 4411
MluI A'CGCG_T
Cuts at: 7048 7048
Size: 7615
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 354 1935 6286 6470 354
Size: 1581 4351 184 1499
Fragments arranged by size:
4351 1581 1499 184
MscI TGG'CCA
Cuts at: 509 1351 1462 2131 2407 3268 4303 5374 509
Size: 842 111 669 276 861 1035 1071 2750
Fragments arranged by size:
2750 1071 1035 861 842 669 276 111
MslI CAynn'nnrTG
Cuts at: 687 942 1490 1732 2971 3760 4208 5602 5729
Size: 255 548 242 1239 789 448 1394 127
Cuts at: 5729 687
Size: 2573
Fragments arranged by size:
2573 1394 1239 789 548 448 255 242
127
MspA1I CmG'CkG
Cuts at: 147 180 219 366 451 660 1233 2205 2389
Size: 33 39 147 85 209 573 972 184
Cuts at: 2389 2862 2937 3115 3831 4065 4290 4380 4554
Size: 473 75 178 716 234 225 90 174
Cuts at: 4554 4724 5038 5398 6413 6658 147
Size: 170 314 360 1015 245 1104
Fragments arranged by size:
1104 1015 972 716 573 473 360 314
245 234 225 209 184 178 174 170
147 90 85 75 39 33
NaeI GCC'GGC
Cuts at: 5794 5794
Size: 7615
NarI GG'CG_CC
Cuts at: 324 2526 2723 3405 5044 5292 324
Size: 2202 197 682 1639 248 2647
Fragments arranged by size:
2647 2202 1639 682 248 197
NcoI C'CATG_G
Cuts at: 125 629 4226 125
Size: 504 3597 3514
Fragments arranged by size:
3597 3514 504
NgoMIV G'CCGG_C
Cuts at: 5792 5792
Size: 7615
NheI G'CTAG_C
Cuts at: 1113 1113
Size: 7615
NruI TCG'CGA
Cuts at: 5976 5976
Size: 7615
NspI r_CATG'y
Cuts at: 2095 2442 4666 5166 6075 2095
Size: 347 2224 500 909 3635
Fragments arranged by size:
3635 2224 909 500 347
PasI CC'CwG_GG
Cuts at: 622 719 3667 3813 3852 4155 622
Size: 97 2948 146 39 303 4082
Fragments arranged by size:
4082 2948 303 146 97 39
PciI A'CATG_T
Cuts at: 6071 6071
Size: 7615
PflMI CCAn_nnn'nTGG
Cuts at: 474 2588 2775 3876 474
Size: 2114 187 1101 4213
Fragments arranged by size:
4213 2114 1101 187
PfoI T'CCnGG_A
Cuts at: 315 1061 1808 3869 315
Size: 746 747 2061 4061
Fragments arranged by size:
4061 2061 747 746
PmeI GTTT'AAAC
Cuts at: 4612 4612
Size: 7615
PmlI CAC'GTG
Cuts at: 2739 3366 2739
Size: 627 6988
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6754 6786 6754
Size: 32 7583
PpuMI rG'GwC_Cy
Cuts at: 412 520 1947 4597 412
Size: 108 1427 2650 3430
Fragments arranged by size:
3430 2650 1427 108
PshAI GACnn'nnGTC
Cuts at: 2607 2607
Size: 7615
PspOMI G'GGCC_C
Cuts at: 625 1030 1237 625
Size: 405 207 7003
Fragments arranged by size:
7003 405 207
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1198 1230 1198
Size: 32 7583
PstI C_TGCA'G
Cuts at: 313 659 890 1148 1902 2702 3830 4562 4660
Size: 346 231 258 754 800 1128 732 98
Cuts at: 4660 5345 313
Size: 685 2583
Fragments arranged by size:
2583 1128 800 754 732 685 346 258
231 98
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7615
PvuII CAG'CTG
Cuts at: 147 219 451 660 1233 2205 2862 2937 3115
Size: 72 232 209 573 972 657 75 178
Cuts at: 3115 3831 4065 4290 4380 4554 5038 5398 147
Size: 716 234 225 90 174 484 360 2364
Fragments arranged by size:
2364 972 716 657 573 484 360 234
232 225 209 178 174 90 75 72
RsrII CG'GwC_CG
Cuts at: 5808 5808
Size: 7615
SacI G_AGCT'C
Cuts at: 848 2047 2075 2363 4627 848
Size: 1199 28 288 2264 3836
Fragments arranged by size:
3836 2264 1199 288 28
SalI G'TCGA_C
Cuts at: 4650 4650
Size: 7615
SanDI GG'GwC_CC
Cuts at: 520 1947 520
Size: 1427 6188
SapI GCTCTTCn'nnn_
Cuts at: 2035 2929 4009 5636 5846 2035
Size: 894 1080 1627 210 3804
Fragments arranged by size:
3804 1627 1080 894 210
SbfI CC_TGCA'GG
Cuts at: 4660 4660
Size: 7615
ScaI AGT'ACT
Cuts at: 7132 7132
Size: 7615
SfcI C'TryA_G
Cuts at: 33 309 655 886 1144 1480 1881 1898 1910
Size: 276 346 231 258 336 401 17 12
Cuts at: 1910 2698 3378 3495 3589 3826 4558 4656 5341
Size: 788 680 117 94 237 732 98 685
Cuts at: 5341 6336 6527 33
Size: 995 191 1121
Fragments arranged by size:
1121 995 788 732 685 680 401 346
336 276 258 237 231 191 117 98
94 17 12
SfiI GGCCn_nnn'nGGCC
Cuts at: 832 3487 832
Size: 2655 4960
SfoI GGC'GCC
Cuts at: 325 2527 2724 3406 5045 5293 325
Size: 2202 197 682 1639 248 2647
Fragments arranged by size:
2647 2202 1639 682 248 197
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7615
SmaI CCC'GGG
Cuts at: 517 4635 517
Size: 4118 3497
SmlI C'TyrA_G
Cuts at: 1595 3526 4768 5683 6177 6439 1595
Size: 1931 1242 915 494 262 2771
Fragments arranged by size:
2771 1931 1242 915 494 262
SnaBI TAC'GTA
Cuts at: 4605 4605
Size: 7615
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7615
SphI G_CATG'C
Cuts at: 2442 4666 5166 2442
Size: 2224 500 4891
Fragments arranged by size:
4891 2224 500
StuI AGG'CCT
Cuts at: 1585 1876 3281 1585
Size: 291 1405 5919
Fragments arranged by size:
5919 1405 291
StyI C'CwwG_G
Cuts at: 125 524 530 629 1730 1789 1826 2114 3455
Size: 399 6 99 1101 59 37 288 1341
Cuts at: 3455 4226 4747 5101 125
Size: 771 521 354 2639
Fragments arranged by size:
2639 1341 1101 771 521 399 354 288
99 59 37 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 229 474 1123 1387 1782 2149 2485 3198 4219
Size: 245 649 264 395 367 336 713 1021
Cuts at: 4219 4240 5196 5329 5788 5999 229
Size: 21 956 133 459 211 1845
Fragments arranged by size:
1845 1021 956 713 649 459 395 367
336 264 245 211 133 21
TatI w'GTAC_w
Cuts at: 1214 2207 2634 3356 3785 7130 1214
Size: 993 427 722 429 3345 1699
Fragments arranged by size:
3345 1699 993 722 429 427
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3411 3443 7132 7164 3411
Size: 32 3689 32 3862
Fragments arranged by size:
3862 3689 32 32
Tth111I GACn'n_nGTC
Cuts at: 5410 5410
Size: 7615
XbaI T'CTAG_A
Cuts at: 4644 4644
Size: 7615
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 716 1455 1824 2083 4233 4356 5108 716
Size: 739 369 259 2150 123 752 3223
Fragments arranged by size:
3223 2150 752 739 369 259 123
XhoI C'TCGA_G
Cuts at: 1595 1595
Size: 7615
XmaI C'CCGG_G
Cuts at: 515 4633 515
Size: 4118 3497
XmnI GAAnn'nnTTC
Cuts at: 134 134
Size: 7615
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbsI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI BstZ17I
Bsu36I BtgI BtgZI BtsI ClaI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NheI NruI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PspOMI PsrI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SfcI SfiI SfoI SgfI SmaI SmlI SnaBI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AflII AscI AvrII BbvCI BcgI BfrBI BsiWI
BsmBI BssHII BstEII CspCI DraIII EcoRV FseI FspAI
HpaI MfeI NdeI NotI NsiI PacI PsiI PspXI
SacII SexAI SgrAI SrfI SspI SwaI ZraI