Restriction map for
pF1KSDF0018
(Circular) (Six-base) MAPSORT of: pF1KSDF0018.seq Check: 5741 from: 1 to: 7297
pF1KSDF0018 7297 bp
With 182 enzymes: SgfI *
June 20, 2008 10:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 546 1301 546
Size: 755 6542
AccI GT'mk_AC
Cuts at: 2874 4333 2874
Size: 1459 5838
Acc65I G'GTAC_C
Cuts at: 3448 4311 3448
Size: 863 6434
AclI AA'CG_TT
Cuts at: 7137 7137
Size: 7297
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1775 1883 2285 3421 3869 5120 5552 6301 6569
Size: 108 402 1136 448 1251 432 749 268
Cuts at: 6569 1775
Size: 2503
Fragments arranged by size:
2503 1251 1136 749 448 432 402 268
108
AflIII A'CryG_T
Cuts at: 2745 2779 2877 4280 4647 5753 6730 2745
Size: 34 98 1403 367 1106 977 3312
Fragments arranged by size:
3312 1403 1106 977 367 98 34
AgeI A'CCGG_T
Cuts at: 5685 5685
Size: 7297
AleI CACnn'nnGTG
Cuts at: 3235 3235
Size: 7297
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1007 1007
Size: 7297
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 396 428 1066 1098 396
Size: 32 638 32 6595
Fragments arranged by size:
6595 638 32 32
AlwNI CAG_nnn'CTG
Cuts at: 238 322 904 1687 2066 3331 3655 3933 4600
Size: 84 582 783 379 1265 324 278 667
Cuts at: 4600 6169 238
Size: 1569 1366
Fragments arranged by size:
1569 1366 1265 783 667 582 379 324
278 84
ApaI G_GGCC'C
Cuts at: 329 517 898 1541 3325 3394 3940 4026 4254
Size: 188 381 643 1784 69 546 86 228
Cuts at: 4254 329
Size: 3372
Fragments arranged by size:
3372 1784 643 546 381 228 188 86
69
ApaLI G'TGCA_C
Cuts at: 6067 6067
Size: 7297
ApoI r'AATT_y
Cuts at: 2027 2054 2072 4299 6650 6906 2027
Size: 27 18 2227 2351 256 2418
Fragments arranged by size:
2418 2351 2227 256 27 18
AseI AT'TA_AT
Cuts at: 20 20
Size: 7297
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7297
AvaI C'yCGr_G
Cuts at: 175 263 446 714 1238 1484 2547 2570 2633
Size: 88 183 268 524 246 1063 23 63
Cuts at: 2633 2666 4037 4315 175
Size: 33 1371 278 3157
Fragments arranged by size:
3157 1371 1063 524 278 268 246 183
88 63 33 23
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3680 3713 3680
Size: 33 7264
BamHI G'GATC_C
Cuts at: 312 404 2606 3381 3491 4320 312
Size: 92 2202 775 110 829 3289
Fragments arranged by size:
3289 2202 829 775 110 92
BanI G'GyrC_C
Cuts at: 211 1845 3021 3448 4311 4725 4973 5008 211
Size: 1634 1176 427 863 414 248 35 2500
Fragments arranged by size:
2500 1634 1176 863 427 414 248 35
BanII G_rGCy'C
Cuts at: 141 280 304 329 362 517 611 721 898
Size: 139 24 25 33 155 94 110 177
Cuts at: 898 1049 1454 1541 1979 2547 2729 3274 3325
Size: 151 405 87 438 568 182 545 51
Cuts at: 3325 3394 3530 3940 4026 4045 4254 4263 4309
Size: 69 136 410 86 19 209 9 46
Cuts at: 4309 5339 141
Size: 1030 2099
Fragments arranged by size:
2099 1030 568 545 438 410 405 209
182 177 155 151 139 136 110 94
87 86 69 51 46 33 25 24
19 9
BbeI G_GCGC'C
Cuts at: 4729 4977 4729
Size: 248 7049
BbsI GAAGACnn'nnnn_
Cuts at: 3875 3875
Size: 7297
BbvCI CC'TCA_GC
Cuts at: 2234 2234
Size: 7297
BciVI GTATCCnnnnn_n'
Cuts at: 5193 5956 7297 5193
Size: 763 1341 5193
Fragments arranged by size:
5193 1341 763
BclI T'GATC_A
Cuts at: 3045 4815 3045
Size: 1770 5527
BglI GCCn_nnn'nGGC
Cuts at: 2440 2864 4258 2440
Size: 424 1394 5479
Fragments arranged by size:
5479 1394 424
BglII A'GATC_T
Cuts at: 4810 6849 4810
Size: 2039 5258
BlpI GC'TnA_GC
Cuts at: 342 544 1613 4123 4407 342
Size: 202 1069 2510 284 3232
Fragments arranged by size:
3232 2510 1069 284 202
Bme1580I G_kGCm'C
Cuts at: 329 517 565 898 1428 1541 2042 2787 3242
Size: 188 48 333 530 113 501 745 455
Cuts at: 3242 3325 3394 3940 4026 4254 4920 5013 6071
Size: 83 69 546 86 228 666 93 1058
Cuts at: 6071 329
Size: 1555
Fragments arranged by size:
1555 1058 745 666 546 530 501 455
333 228 188 113 93 86 83 69
48
BmgBI CAC'GTC
Cuts at: 1965 2037 4064 1965
Size: 72 2027 5198
Fragments arranged by size:
5198 2027 72
BmrI ACTGGGnnnn_n'
Cuts at: 894 914 2162 2296 3808 4507 4588 4923 7069
Size: 20 1248 134 1512 699 81 335 2146
Cuts at: 7069 894
Size: 1122
Fragments arranged by size:
2146 1512 1248 1122 699 335 134 81
20
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5662 5662
Size: 7297
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 317 333 454 634 988 1201 1282 1327 1643
Size: 16 121 180 354 213 81 45 316
Cuts at: 1643 1726 1951 2225 2518 2957 3001 3167 3942
Size: 83 225 274 293 439 44 166 775
Cuts at: 3942 317
Size: 3672
Fragments arranged by size:
3672 775 439 354 316 293 274 225
213 180 166 121 83 81 45 44
16
Bpu10I CC'TnA_GC
Cuts at: 289 1219 2234 2894 2942 3092 3143 3855 289
Size: 930 1015 660 48 150 51 712 3731
Fragments arranged by size:
3731 1015 930 712 660 150 51 48
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 411 832 4471 5350 5844 6142 411
Size: 421 3639 879 494 298 1566
Fragments arranged by size:
3639 1566 879 494 421 298
BsaI GGTCTCn'nnnn_
Cuts at: 1235 1478 7001 1235
Size: 243 5523 1531
Fragments arranged by size:
5523 1531 243
BsaAI yAC'GTr
Cuts at: 2748 4152 4287 4648 2748
Size: 1404 135 361 5397
Fragments arranged by size:
5397 1404 361 135
BsaBI GATnn'nnATC
Cuts at: 6 2507 4809 6848 6
Size: 2501 2302 2039 455
Fragments arranged by size:
2501 2302 2039 455
BsaHI Gr'CG_yC
Cuts at: 2566 3895 4726 4974 5678 7174 2566
Size: 1329 831 248 704 1496 2689
Fragments arranged by size:
2689 1496 1329 831 704 248
BsaWI w'CCGG_w
Cuts at: 396 1036 2426 3669 4193 4484 5005 5685 5959
Size: 640 1390 1243 524 291 521 680 274
Cuts at: 5959 6106 6517 396
Size: 147 411 1176
Fragments arranged by size:
1390 1243 1176 680 640 524 521 411
291 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 703 730 733 760 1066 1096 1280 1310 1357
Size: 27 3 27 306 30 184 30 47
Cuts at: 1357 1387 703
Size: 30 6613
Fragments arranged by size:
6613 306 184 47 30 30 30 27
27 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 684 1103 1466 1921 1924 1927 2338 3791 684
Size: 419 363 455 3 3 411 1453 4190
Fragments arranged by size:
4190 1453 455 419 411 363 3 3
BseYI C'CCAG_C
Cuts at: 788 1023 1885 2005 2059 2684 3495 4088 4094
Size: 235 862 120 54 625 811 593 6
Cuts at: 4094 4720 5478 6057 6553 788
Size: 626 758 579 496 1532
Fragments arranged by size:
1532 862 811 758 626 625 593 579
496 235 120 54 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 685 1033 1673 2995 3449 3641 685
Size: 348 640 1322 454 192 4341
Fragments arranged by size:
4341 1322 640 454 348 192
BsiEI CG_ry'CG
Cuts at: 104 4837 4883 6093 104
Size: 4733 46 1210 1308
Fragments arranged by size:
4733 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 280 295 611 721 1049 3530 4309 5087 5277
Size: 15 316 110 328 2481 779 778 190
Cuts at: 5277 6071 280
Size: 794 1506
Fragments arranged by size:
2481 1506 794 779 778 328 316 190
110 15
BsmI GAATG_Cn'
Cuts at: 842 2009 2404 842
Size: 1167 395 5735
Fragments arranged by size:
5735 1167 395
Bsp1286I G_dGCh'C
Cuts at: 141 280 295 304 329 362 517 565 611
Size: 139 15 9 25 33 155 48 46
Cuts at: 611 721 898 1049 1428 1454 1541 1979 2042
Size: 110 177 151 379 26 87 438 63
Cuts at: 2042 2547 2729 2787 3242 3274 3325 3394 3530
Size: 505 182 58 455 32 51 69 136
Cuts at: 3530 3940 4026 4045 4254 4263 4309 4920 5013
Size: 410 86 19 209 9 46 611 93
Cuts at: 5013 5087 5277 5339 6071 141
Size: 74 190 62 732 1367
Fragments arranged by size:
1367 732 611 505 455 438 410 379
209 190 182 177 155 151 139 136
110 93 87 86 74 69 63 62
58 51 48 46 46 33 32 26
25 19 15 9 9
BspEI T'CCGG_A
Cuts at: 2426 3669 6517 2426
Size: 1243 2848 3206
Fragments arranged by size:
3206 2848 1243
BspHI T'CATG_A
Cuts at: 6473 6473
Size: 7297
BspMI ACCTGCnnnn'nnnn_
Cuts at: 546 1301 4345 4559 4861 5242 546
Size: 755 3044 214 302 381 2601
Fragments arranged by size:
3044 2601 755 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1128 2411 2531 2604 5587 5641 7127 7292
Size: 1078 1283 120 73 2983 54 1486 165
Cuts at: 7292 50
Size: 55
Fragments arranged by size:
2983 1486 1283 1078 165 120 73 55
54
BsrDI GCAATG_nn'
Cuts at: 1988 5207 1988
Size: 3219 4078
BsrFI r'CCGG_y
Cuts at: 1575 2660 3024 5293 5474 5685 1575
Size: 1085 364 2269 181 211 3187
Fragments arranged by size:
3187 2269 1085 364 211 181
BssHII G'CGCG_C
Cuts at: 997 997
Size: 7297
BssSI C'ACGA_G
Cuts at: 5566 5926 5566
Size: 360 6937
BstAPI GCAn_nnn'nTGC
Cuts at: 2015 4672 2015
Size: 2657 4640
BstBI TT'CG_AA
Cuts at: 110 110
Size: 7297
BstEII G'GTnAC_C
Cuts at: 3253 3253
Size: 7297
BstXI CCAn_nnnn'nTGG
Cuts at: 1849 3966 1849
Size: 2117 5180
BstYI r'GATC_y
Cuts at: 1 312 404 1794 2508 2606 3381 3491 4320
Size: 311 92 1390 714 98 775 110 829
Cuts at: 4320 4787 4810 5145 5391 6405 6491 6503 6849
Size: 467 23 335 246 1014 86 12 346
Cuts at: 6849 1
Size: 449
Fragments arranged by size:
1390 1014 829 775 714 467 449 346
335 311 246 110 98 92 86 23
12
Bsu36I CC'TnA_GG
Cuts at: 131 1697 131
Size: 1566 5731
BtgI C'CryG_G
Cuts at: 251 3827 251
Size: 3576 3721
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 5245 5426 5589 5245
Size: 181 163 6953
Fragments arranged by size:
6953 181 163
BtsI GCAGTG_nn'
Cuts at: 2485 4538 2485
Size: 2053 5244
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 3497 3532 3497
Size: 35 7262
DraI TTT'AAA
Cuts at: 4294 4294
Size: 7297
DraIII CAC_nnn'GTG
Cuts at: 218 218
Size: 7297
DrdI GACnn_nn'nnGTC
Cuts at: 532 1591 3592 5001 5861 532
Size: 1059 2001 1409 860 1968
Fragments arranged by size:
2001 1968 1409 1059 860
EaeI y'GGCC_r
Cuts at: 183 1163 2467 2865 4174 4880 5054 5445 5472
Size: 980 1304 398 1309 706 174 391 27
Cuts at: 5472 6819 183
Size: 1347 661
Fragments arranged by size:
1347 1309 1304 980 706 661 398 391
174 27
EagI C'GGCC_G
Cuts at: 4880 4880
Size: 7297
EarI CTCTTCn'nnn_
Cuts at: 183 1141 1310 1315 1357 1898 1902 1913 3721
Size: 958 169 5 42 541 4 11 1808
Cuts at: 3721 5318 5528 183
Size: 1597 210 1952
Fragments arranged by size:
1952 1808 1597 958 541 210 169 42
11 5 4
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 370 743 904 1109 2641 5503 5815 5961 7107
Size: 373 161 205 1532 2862 312 146 1146
Cuts at: 7107 370
Size: 560
Fragments arranged by size:
2862 1532 1146 560 373 312 205 161
146
EcoICRI GAG'CTC
Cuts at: 278 609 719 1047 3528 4307 278
Size: 331 110 328 2481 779 3268
Fragments arranged by size:
3268 2481 779 331 328 110
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 317 333 454 634 988 1201 1282 1327 1643
Size: 16 121 180 354 213 81 45 316
Cuts at: 1643 1726 1775 1883 1951 2225 2285 2518 2957
Size: 83 49 108 68 274 60 233 439
Cuts at: 2957 3001 3167 3421 3869 3942 5120 5552 6301
Size: 44 166 254 448 73 1178 432 749
Cuts at: 6301 6569 317
Size: 268 1045
Fragments arranged by size:
1178 1045 749 448 439 432 354 316
274 268 254 233 213 180 166 121
108 83 81 73 68 60 49 45
44 16
EcoNI CCTnn'n_nnAGG
Cuts at: 477 1297 2303 2945 3773 4016 477
Size: 820 1006 642 828 243 3758
Fragments arranged by size:
3758 1006 828 820 642 243
EcoO109I rG'GnC_Cy
Cuts at: 370 483 514 534 894 1538 1584 1701 1742
Size: 113 31 20 360 644 46 117 41
Cuts at: 1742 1948 2215 2806 3004 3321 3390 3798 3937
Size: 206 267 591 198 317 69 408 139
Cuts at: 3937 4009 4022 4023 4250 4434 370
Size: 72 13 1 227 184 3233
Fragments arranged by size:
3233 644 591 408 360 317 267 227
206 198 184 139 117 113 72 69
46 41 31 20 13 1
EcoRI G'AATT_C
Cuts at: 4299 4299
Size: 7297
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1420 5314 1420
Size: 3894 3403
FspI TGC'GCA
Cuts at: 5076 5076
Size: 7297
HaeII r_GCGC'y
Cuts at: 641 783 1350 2435 4729 4977 6001 6980 641
Size: 142 567 1085 2294 248 1024 979 958
Fragments arranged by size:
2294 1085 1024 979 958 567 248 142
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 137 169 1031 1063 1218 1250 1280 1312 1323
Size: 32 862 32 155 32 30 32 11
Cuts at: 1323 1355 3885 3917 5137 5167 5169 5199 137
Size: 32 2530 32 1220 30 2 30 2235
Fragments arranged by size:
2530 2235 1220 862 155 32 32 32
32 32 32 30 30 30 11 2
HincII GTy'rAC
Cuts at: 3343 4143 4334 3343
Size: 800 191 6306
Fragments arranged by size:
6306 800 191
HpaI GTT'AAC
Cuts at: 4143 4143
Size: 7297
KasI G'GCGC_C
Cuts at: 4725 4973 4725
Size: 248 7049
KpnI G_GTAC'C
Cuts at: 3452 4315 3452
Size: 863 6434
MluI A'CGCG_T
Cuts at: 6730 6730
Size: 7297
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1052 3698 3857 5968 6152 1052
Size: 2646 159 2111 184 2197
Fragments arranged by size:
2646 2197 2111 184 159
MscI TGG'CCA
Cuts at: 1165 2867 5056 1165
Size: 1702 2189 3406
Fragments arranged by size:
3406 2189 1702
MslI CAynn'nnrTG
Cuts at: 2778 3235 4150 5284 5411 2778
Size: 457 915 1134 127 4664
Fragments arranged by size:
4664 1134 915 457 127
MspA1I CmG'CkG
Cuts at: 949 1017 1996 2066 2233 2460 2525 2622 2926
Size: 68 979 70 167 227 65 97 304
Cuts at: 2926 3652 3754 3949 4406 4720 5080 6095 6340
Size: 726 102 195 457 314 360 1015 245
Cuts at: 6340 949
Size: 1906
Fragments arranged by size:
1906 1015 979 726 457 360 314 304
245 227 195 167 102 97 70 68
65
NaeI GCC'GGC
Cuts at: 2662 3026 5476 2662
Size: 364 2450 4483
Fragments arranged by size:
4483 2450 364
NarI GG'CG_CC
Cuts at: 4726 4974 4726
Size: 248 7049
NcoI C'CATG_G
Cuts at: 251 3827 251
Size: 3576 3721
NgoMIV G'CCGG_C
Cuts at: 2660 3024 5474 2660
Size: 364 2450 4483
Fragments arranged by size:
4483 2450 364
NruI TCG'CGA
Cuts at: 914 5658 914
Size: 4744 2553
NspI r_CATG'y
Cuts at: 994 2783 2881 2965 4284 4348 4848 5757 994
Size: 1789 98 84 1319 64 500 909 2534
Fragments arranged by size:
2534 1789 1319 909 500 98 84 64
PasI CC'CwG_GG
Cuts at: 322 1507 2802 3387 4254 322
Size: 1185 1295 585 867 3365
Fragments arranged by size:
3365 1295 1185 867 585
PciI A'CATG_T
Cuts at: 2779 2877 4280 5753 2779
Size: 98 1403 1473 4323
Fragments arranged by size:
4323 1473 1403 98
PflMI CCAn_nnn'nTGG
Cuts at: 322 904 1534 1968 3993 322
Size: 582 630 434 2025 3626
Fragments arranged by size:
3626 2025 630 582 434
PfoI T'CCnGG_A
Cuts at: 611 734 1004 1763 1967 2495 611
Size: 123 270 759 204 528 5413
Fragments arranged by size:
5413 759 528 270 204 123
PmeI GTTT'AAAC
Cuts at: 4294 4294
Size: 7297
PmlI CAC'GTG
Cuts at: 2748 4152 2748
Size: 1404 5893
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1066 1098 6436 6468 1066
Size: 32 5338 32 1895
Fragments arranged by size:
5338 1895 32 32
PpuMI rG'GwC_Cy
Cuts at: 483 534 1584 1701 1742 2806 483
Size: 51 1050 117 41 1064 4974
Fragments arranged by size:
4974 1064 1050 117 51 41
PspOMI G'GGCC_C
Cuts at: 325 513 894 1537 3321 3390 3936 4022 4250
Size: 188 381 643 1784 69 546 86 228
Cuts at: 4250 325
Size: 3372
Fragments arranged by size:
3372 1784 643 546 381 228 188 86
69
PstI C_TGCA'G
Cuts at: 243 776 815 869 2225 3340 4342 5027 243
Size: 533 39 54 1356 1115 1002 685 2513
Fragments arranged by size:
2513 1356 1115 1002 685 533 54 39
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7297
PvuII CAG'CTG
Cuts at: 1996 2233 2460 2926 3754 3949 4720 5080 1996
Size: 237 227 466 828 195 771 360 4213
Fragments arranged by size:
4213 828 771 466 360 237 227 195
RsrII CG'GwC_CG
Cuts at: 5490 5490
Size: 7297
SacI G_AGCT'C
Cuts at: 280 611 721 1049 3530 4309 280
Size: 331 110 328 2481 779 3268
Fragments arranged by size:
3268 2481 779 331 328 110
SalI G'TCGA_C
Cuts at: 4332 4332
Size: 7297
SapI GCTCTTCn'nnn_
Cuts at: 1141 3721 5318 5528 1141
Size: 2580 1597 210 2910
Fragments arranged by size:
2910 2580 1597 210
SbfI CC_TGCA'GG
Cuts at: 4342 4342
Size: 7297
ScaI AGT'ACT
Cuts at: 6814 6814
Size: 7297
SfcI C'TryA_G
Cuts at: 33 239 772 811 865 2221 3336 4338 5023
Size: 206 533 39 54 1356 1115 1002 685
Cuts at: 5023 6018 6209 33
Size: 995 191 1121
Fragments arranged by size:
1356 1121 1115 1002 995 685 533 206
191 54 39
SfoI GGC'GCC
Cuts at: 4727 4975 4727
Size: 248 7049
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7297
SmaI CCC'GGG
Cuts at: 448 4039 4317 448
Size: 3591 278 3428
Fragments arranged by size:
3591 3428 278
SmlI C'TyrA_G
Cuts at: 426 847 1484 4450 5365 5859 6121 426
Size: 421 637 2966 915 494 262 1602
Fragments arranged by size:
2966 1602 915 637 494 421 262
SnaBI TAC'GTA
Cuts at: 4287 4287
Size: 7297
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7297
SphI G_CATG'C
Cuts at: 994 4348 4848 994
Size: 3354 500 3443
Fragments arranged by size:
3443 3354 500
StuI AGG'CCT
Cuts at: 1787 1787
Size: 7297
StyI C'CwwG_G
Cuts at: 141 251 1385 2211 3194 3827 4181 4429 4783
Size: 110 1134 826 983 633 354 248 354
Cuts at: 4783 141
Size: 2655
Fragments arranged by size:
2655 1134 983 826 633 354 354 248
110
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1015 1825 2389 2503 3071 3630 3822 4878 5011
Size: 810 564 114 568 559 192 1056 133
Cuts at: 5011 5470 5681 1015
Size: 459 211 2631
Fragments arranged by size:
2631 1056 810 568 564 559 459 211
192 133 114
TatI w'GTAC_w
Cuts at: 698 1184 6812 698
Size: 486 5628 1183
Fragments arranged by size:
5628 1183 486
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1357 1389 1949 1981 2477 2509 3528 3560 6814
Size: 32 560 32 496 32 1019 32 3254
Cuts at: 6814 6846 1357
Size: 32 1808
Fragments arranged by size:
3254 1808 1019 560 496 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 2565 3331 5092 2565
Size: 766 1761 4770
Fragments arranged by size:
4770 1761 766
XbaI T'CTAG_A
Cuts at: 3786 4326 3786
Size: 540 6757
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 764 4790 764
Size: 4026 3271
XhoI C'TCGA_G
Cuts at: 1484 1484
Size: 7297
XmaI C'CCGG_G
Cuts at: 446 4037 4315 446
Size: 3591 278 3428
Fragments arranged by size:
3591 3428 278
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HpaI KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NruI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SnaBI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AscI AvrII BcgI BfrBI
BmtI BsiWI BsmBI BsrGI BstZ17I ClaI EcoRV FseI
FspAI HindIII MfeI NdeI NheI NotI NsiI PacI
PshAI PsiI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SrfI SspI SwaI XmnI ZraI