Restriction map for
pF1KSDF0044
(Circular) (Six-base) MAPSORT of: pF1KSDF0044.seq Check: 4423 from: 1 to: 6106
pF1KSDF0044 6106 bp
With 182 enzymes: SgfI *
June 20, 2008 10:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 267 449 906 1205 1336 1653 2142 267
Size: 182 457 299 131 317 489 4231
Fragments arranged by size:
4231 489 457 317 299 182 131
AatII G_ACGT'C
Cuts at: 1235 1235
Size: 6106
AccI GT'mk_AC
Cuts at: 3142 3142
Size: 6106
Acc65I G'GTAC_C
Cuts at: 3120 3120
Size: 6106
AclI AA'CG_TT
Cuts at: 5946 5946
Size: 6106
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1692 3929 4361 5110 5378 1692
Size: 2237 432 749 268 2420
Fragments arranged by size:
2420 2237 749 432 268
AfeI AGC'GCT
Cuts at: 787 787
Size: 6106
AflIII A'CryG_T
Cuts at: 3456 4562 5539 3456
Size: 1106 977 4023
Fragments arranged by size:
4023 1106 977
AgeI A'CCGG_T
Cuts at: 4494 4494
Size: 6106
AleI CACnn'nnGTG
Cuts at: 580 766 1757 2287 580
Size: 186 991 530 4399
Fragments arranged by size:
4399 991 530 186
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1775 1775
Size: 6106
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 274 306 1714 1746 2257 2289 274
Size: 32 1408 32 511 32 4091
Fragments arranged by size:
4091 1408 511 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 930 966 1056 1620 1668 2871 3409 4978 930
Size: 36 90 564 48 1203 538 1569 2058
Fragments arranged by size:
2058 1569 1203 564 538 90 48 36
ApaI G_GGCC'C
Cuts at: 1143 1566 2809 1143
Size: 423 1243 4440
Fragments arranged by size:
4440 1243 423
ApaLI G'TGCA_C
Cuts at: 4876 4876
Size: 6106
ApoI r'AATT_y
Cuts at: 2956 3108 5459 5715 2956
Size: 152 2351 256 3347
Fragments arranged by size:
3347 2351 256 152
AseI AT'TA_AT
Cuts at: 20 20
Size: 6106
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6106
AvaI C'yCGr_G
Cuts at: 172 220 296 413 529 1453 1492 2972 3124
Size: 48 76 117 116 924 39 1480 152
Cuts at: 3124 172
Size: 3154
Fragments arranged by size:
3154 1480 924 152 117 116 76 48
39
BamHI G'GATC_C
Cuts at: 3129 3129
Size: 6106
BanI G'GyrC_C
Cuts at: 300 417 838 887 1358 2320 2793 2842 2853
Size: 117 421 49 471 962 473 49 11
Cuts at: 2853 2991 3002 3120 3534 3782 3817 300
Size: 138 11 118 414 248 35 2589
Fragments arranged by size:
2589 962 473 471 421 414 248 138
118 117 49 49 35 11 11
BanII G_rGCy'C
Cuts at: 311 530 817 1143 1168 1324 1501 1566 2023
Size: 219 287 326 25 156 177 65 457
Cuts at: 2023 2378 2711 2809 3118 4148 311
Size: 355 333 98 309 1030 2269
Fragments arranged by size:
2269 1030 457 355 333 326 309 287
219 177 156 98 65 25
BbeI G_GCGC'C
Cuts at: 2797 2857 2995 3538 3786 2797
Size: 60 138 543 248 5117
Fragments arranged by size:
5117 543 248 138 60
BbvCI CC'TCA_GC
Cuts at: 238 238
Size: 6106
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 391 425 2695 2729 391
Size: 34 2270 34 3768
Fragments arranged by size:
3768 2270 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4002 4765 6106 4002
Size: 763 1341 4002
Fragments arranged by size:
4002 1341 763
BclI T'GATC_A
Cuts at: 3624 3624
Size: 6106
BglI GCCn_nnn'nGGC
Cuts at: 1575 2482 2851 3000 1575
Size: 907 369 149 4681
Fragments arranged by size:
4681 907 369 149
BglII A'GATC_T
Cuts at: 3619 5658 3619
Size: 2039 4067
BlpI GC'TnA_GC
Cuts at: 782 2620 3216 782
Size: 1838 596 3672
Fragments arranged by size:
3672 1838 596
Bme1580I G_kGCm'C
Cuts at: 422 624 843 1143 1361 1493 1566 1748 1764
Size: 202 219 300 218 132 73 182 16
Cuts at: 1764 2323 2754 2809 2820 3005 3729 3822 4880
Size: 559 431 55 11 185 724 93 1058
Cuts at: 4880 422
Size: 1648
Fragments arranged by size:
1648 1058 724 559 431 300 219 218
202 185 182 132 93 73 55 16
11
BmgBI CAC'GTC
Cuts at: 578 2664 578
Size: 2086 4020
BmrI ACTGGGnnnn_n'
Cuts at: 2026 2848 3316 3397 3732 5878 2026
Size: 822 468 81 335 2146 2254
Fragments arranged by size:
2254 2146 822 468 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 309 717 4471 309
Size: 408 3754 1944
Fragments arranged by size:
3754 1944 408
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 430 542 1186 1297 1420 1435 1549 1651 1867
Size: 112 644 111 123 15 114 102 216
Cuts at: 1867 1886 1960 2158 2992 430
Size: 19 74 198 834 3544
Fragments arranged by size:
3544 834 644 216 198 123 114 112
111 102 74 19 15
Bpu10I CC'TnA_GC
Cuts at: 238 305 490 1162 238
Size: 67 185 672 5182
Fragments arranged by size:
5182 672 185 67
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3280 4159 4653 4951 3280
Size: 879 494 298 4435
Fragments arranged by size:
4435 879 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 1936 1985 2971 5810 1936
Size: 49 986 2839 2232
Fragments arranged by size:
2839 2232 986 49
BsaAI yAC'GTr
Cuts at: 627 1482 3096 3457 627
Size: 855 1614 361 3276
Fragments arranged by size:
3276 1614 855 361
BsaBI GATnn'nnATC
Cuts at: 6 2254 3618 5657 6
Size: 2248 1364 2039 455
Fragments arranged by size:
2248 2039 1364 455
BsaHI Gr'CG_yC
Cuts at: 875 1232 2075 2794 2854 2992 3535 3783 4487
Size: 357 843 719 60 138 543 248 704
Cuts at: 4487 5983 875
Size: 1496 998
Fragments arranged by size:
1496 998 843 719 704 543 357 248
138 60
BsaWI w'CCGG_w
Cuts at: 3293 3814 4494 4768 4915 5326 3293
Size: 521 680 274 147 411 4073
Fragments arranged by size:
4073 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 274 304 532 562 1564 1594 1627 1657 1714
Size: 30 228 30 1002 30 33 30 57
Cuts at: 1714 1744 274
Size: 30 4636
Fragments arranged by size:
4636 1002 228 57 33 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 190 298 913 1432 1435 1624 1648 2386 190
Size: 108 615 519 3 189 24 738 3910
Fragments arranged by size:
3910 738 615 519 189 108 24 3
BseYI C'CCAG_C
Cuts at: 263 378 810 1134 1273 1542 1548 1879 2124
Size: 115 432 324 139 269 6 331 245
Cuts at: 2124 2142 3529 4287 4866 5362 263
Size: 18 1387 758 579 496 1007
Fragments arranged by size:
1387 1007 758 579 496 432 331 324
269 245 139 115 18 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 421 757 1681 1990 421
Size: 336 924 309 4537
Fragments arranged by size:
4537 924 336 309
BsiEI CG_ry'CG
Cuts at: 104 1982 2516 2850 3646 3692 4902 104
Size: 1878 534 334 796 46 1210 1308
Fragments arranged by size:
1878 1308 1210 796 534 334 46
BsiHKAI G_wGCw'C
Cuts at: 407 2378 2567 2711 3118 3896 4086 4880 407
Size: 1971 189 144 407 778 190 794 1633
Fragments arranged by size:
1971 1633 794 778 407 190 189 144
BsmI GAATG_Cn'
Cuts at: 650 1010 650
Size: 360 5746
BsmBI CGTCTCn'nnnn_
Cuts at: 688 688
Size: 6106
Bsp1286I G_dGCh'C
Cuts at: 311 407 422 530 624 817 843 1143 1168
Size: 96 15 108 94 193 26 300 25
Cuts at: 1168 1324 1361 1493 1501 1566 1748 1764 2023
Size: 156 37 132 8 65 182 16 259
Cuts at: 2023 2323 2378 2567 2711 2754 2809 2820 3005
Size: 300 55 189 144 43 55 11 185
Cuts at: 3005 3118 3729 3822 3896 4086 4148 4880 311
Size: 113 611 93 74 190 62 732 1537
Fragments arranged by size:
1537 732 611 300 300 259 193 190
189 185 182 156 144 132 113 108
96 94 93 74 65 62 55 55
43 37 26 25 16 15 11 8
BspEI T'CCGG_A
Cuts at: 5326 5326
Size: 6106
BspHI T'CATG_A
Cuts at: 1040 1791 1898 5282 1040
Size: 751 107 3384 1864
Fragments arranged by size:
3384 1864 751 107
BspMI ACCTGCnnnn'nnnn_
Cuts at: 267 449 906 1205 1336 1653 2142 3154 3368
Size: 182 457 299 131 317 489 1012 214
Cuts at: 3368 3670 4051 267
Size: 302 381 2322
Fragments arranged by size:
2322 1012 489 457 381 317 302 299
214 182 131
BsrBI CCG'CTC
Cuts at: 50 288 452 494 1653 2580 2859 4396 4450
Size: 238 164 42 1159 927 279 1537 54
Cuts at: 4450 5936 6101 50
Size: 1486 165 55
Fragments arranged by size:
1537 1486 1159 927 279 238 165 164
55 54 42
BsrDI GCAATG_nn'
Cuts at: 4016 4016
Size: 6106
BsrFI r'CCGG_y
Cuts at: 215 856 1115 2515 2699 4102 4283 4494 215
Size: 641 259 1400 184 1403 181 211 1827
Fragments arranged by size:
1827 1403 1400 641 259 211 184 181
BssHII G'CGCG_C
Cuts at: 1024 1024
Size: 6106
BssSI C'ACGA_G
Cuts at: 2704 4375 4735 2704
Size: 1671 360 4075
Fragments arranged by size:
4075 1671 360
BstAPI GCAn_nnn'nTGC
Cuts at: 1056 1668 1861 2157 3481 1056
Size: 612 193 296 1324 3681
Fragments arranged by size:
3681 1324 612 296 193
BstBI TT'CG_AA
Cuts at: 110 110
Size: 6106
BstEII G'GTnAC_C
Cuts at: 382 382
Size: 6106
BstXI CCAn_nnnn'nTGG
Cuts at: 1887 2124 2838 1887
Size: 237 714 5155
Fragments arranged by size:
5155 714 237
BstYI r'GATC_y
Cuts at: 1 800 905 3129 3596 3619 3954 4200 5214
Size: 799 105 2224 467 23 335 246 1014
Cuts at: 5214 5300 5312 5658 1
Size: 86 12 346 449
Fragments arranged by size:
2224 1014 799 467 449 346 335 246
105 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 2811 2811
Size: 6106
BtgI C'CryG_G
Cuts at: 125 137 164 429 458 1289 2568 2932 125
Size: 12 27 265 29 831 1279 364 3299
Fragments arranged by size:
3299 1279 831 364 265 29 27 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 197 2115 2345 2428 2610 4054 4235 4398 197
Size: 1918 230 83 182 1444 181 163 1905
Fragments arranged by size:
1918 1905 1444 230 182 181 163 83
BtsI GCAGTG_nn'
Cuts at: 354 776 1170 1537 1780 2318 2413 3347 354
Size: 422 394 367 243 538 95 934 3113
Fragments arranged by size:
3113 934 538 422 394 367 243 95
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 2214 2249 2214
Size: 35 6071
DraI TTT'AAA
Cuts at: 3103 3103
Size: 6106
DraIII CAC_nnn'GTG
Cuts at: 349 630 669 2842 349
Size: 281 39 2173 3613
Fragments arranged by size:
3613 2173 281 39
DrdI GACnn_nn'nnGTC
Cuts at: 2881 3810 4670 2881
Size: 929 860 4317
Fragments arranged by size:
4317 929 860
EaeI y'GGCC_r
Cuts at: 140 207 1932 1979 2513 3689 3863 4254 4281
Size: 67 1725 47 534 1176 174 391 27
Cuts at: 4281 5628 140
Size: 1347 618
Fragments arranged by size:
1725 1347 1176 618 534 391 174 67
47 27
EagI C'GGCC_G
Cuts at: 1979 2513 3689 1979
Size: 534 1176 4396
Fragments arranged by size:
4396 1176 534
EarI CTCTTCn'nnn_
Cuts at: 616 1241 2545 4127 4337 616
Size: 625 1304 1582 210 2385
Fragments arranged by size:
2385 1582 1304 625 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2205 4312 4624 4770 5916 2205
Size: 2107 312 146 1146 2395
Fragments arranged by size:
2395 2107 1146 312 146
EcoICRI GAG'CTC
Cuts at: 2376 2709 3116 2376
Size: 333 407 5366
Fragments arranged by size:
5366 407 333
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 430 542 1186 1297 1420 1435 1549 1651 1692
Size: 112 644 111 123 15 114 102 41
Cuts at: 1692 1867 1886 1960 2158 2992 3929 4361 5110
Size: 175 19 74 198 834 937 432 749
Cuts at: 5110 5378 430
Size: 268 1158
Fragments arranged by size:
1158 937 834 749 644 432 268 198
175 123 114 112 111 102 74 41
19 15
EcoNI CCTnn'n_nnAGG
Cuts at: 895 1570 1630 2965 895
Size: 675 60 1335 4036
Fragments arranged by size:
4036 1335 675 60
EcoO109I rG'GnC_Cy
Cuts at: 169 1139 1140 1450 1563 1702 2421 2806 3243
Size: 970 1 310 113 139 719 385 437
Cuts at: 3243 169
Size: 3032
Fragments arranged by size:
3032 970 719 437 385 310 139 113
1
EcoRI G'AATT_C
Cuts at: 2956 3108 2956
Size: 152 5954
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4123 4123
Size: 6106
FseI GG_CCGG'CC
Cuts at: 219 219
Size: 6106
FspI TGC'GCA
Cuts at: 1603 1672 3885 1603
Size: 69 2213 3824
Fragments arranged by size:
3824 2213 69
HaeII r_GCGC'y
Cuts at: 229 245 537 755 789 1396 1528 2105 2797
Size: 16 292 218 34 607 132 577 692
Cuts at: 2797 2857 2995 3538 3786 4810 5789 229
Size: 60 138 543 248 1024 979 546
Fragments arranged by size:
1024 979 692 607 577 546 543 292
248 218 138 132 60 34 16
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 3946 3976 3978 4008 3946
Size: 30 2 30 6044
Fragments arranged by size:
6044 30 30 2
HincII GTy'rAC
Cuts at: 1976 2658 3143 1976
Size: 682 485 4939
Fragments arranged by size:
4939 682 485
KasI G'GCGC_C
Cuts at: 2793 2853 2991 3534 3782 2793
Size: 60 138 543 248 5117
Fragments arranged by size:
5117 543 248 138 60
KpnI G_GTAC'C
Cuts at: 3124 3124
Size: 6106
MluI A'CGCG_T
Cuts at: 5539 5539
Size: 6106
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1040 1625 4777 4961 1040
Size: 585 3152 184 2185
Fragments arranged by size:
3152 2185 585 184
MscI TGG'CCA
Cuts at: 1934 3865 1934
Size: 1931 4175
MslI CAynn'nnrTG
Cuts at: 580 766 1757 2287 4093 4220 580
Size: 186 991 530 1806 127 2466
Fragments arranged by size:
2466 1806 991 530 186 127
MspA1I CmG'CkG
Cuts at: 139 166 431 723 966 1560 1617 1887 1919
Size: 27 265 292 243 594 57 270 32
Cuts at: 1919 2219 2352 2570 2780 2934 3215 3529 3889
Size: 300 133 218 210 154 281 314 360
Cuts at: 3889 4904 5149 139
Size: 1015 245 1096
Fragments arranged by size:
1096 1015 594 360 314 300 292 281
270 265 245 243 218 210 154 133
57 32 27
NaeI GCC'GGC
Cuts at: 217 858 1117 2517 2701 4285 217
Size: 641 259 1400 184 1584 2038
Fragments arranged by size:
2038 1584 1400 641 259 184
NarI GG'CG_CC
Cuts at: 2794 2854 2992 3535 3783 2794
Size: 60 138 543 248 5117
Fragments arranged by size:
5117 543 248 138 60
NcoI C'CATG_G
Cuts at: 125 1289 125
Size: 1164 4942
NgoMIV G'CCGG_C
Cuts at: 215 856 1115 2515 2699 4283 215
Size: 641 259 1400 184 1584 2038
Fragments arranged by size:
2038 1584 1400 641 259 184
NruI TCG'CGA
Cuts at: 4467 4467
Size: 6106
NspI r_CATG'y
Cuts at: 1032 3157 3657 4566 1032
Size: 2125 500 909 2572
Fragments arranged by size:
2572 2125 909 500
PasI CC'CwG_GG
Cuts at: 1501 2694 1501
Size: 1193 4913
PciI A'CATG_T
Cuts at: 4562 4562
Size: 6106
PflMI CCAn_nnn'nTGG
Cuts at: 630 669 884 2694 2871 630
Size: 39 215 1810 177 3865
Fragments arranged by size:
3865 1810 215 177 39
PfoI T'CCnGG_A
Cuts at: 519 908 1397 1733 2870 519
Size: 389 489 336 1137 3755
Fragments arranged by size:
3755 1137 489 389 336
PmeI GTTT'AAAC
Cuts at: 3103 3103
Size: 6106
PmlI CAC'GTG
Cuts at: 627 1482 627
Size: 855 5251
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 274 306 1714 1746 2026 2058 5245 5277 274
Size: 32 1408 32 280 32 3187 32 1103
Fragments arranged by size:
3187 1408 1103 280 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 169 1702 2421 169
Size: 1533 719 3854
Fragments arranged by size:
3854 1533 719
PspOMI G'GGCC_C
Cuts at: 1139 1562 2805 1139
Size: 423 1243 4440
Fragments arranged by size:
4440 1243 423
PstI C_TGCA'G
Cuts at: 728 1061 1283 1390 2153 2351 3151 3836 728
Size: 333 222 107 763 198 800 685 2998
Fragments arranged by size:
2998 800 763 685 333 222 198 107
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6106
PvuII CAG'CTG
Cuts at: 723 966 1617 2219 2352 3529 3889 723
Size: 243 651 602 133 1177 360 2940
Fragments arranged by size:
2940 1177 651 602 360 243 133
RsrII CG'GwC_CG
Cuts at: 4299 4299
Size: 6106
SacI G_AGCT'C
Cuts at: 2378 2711 3118 2378
Size: 333 407 5366
Fragments arranged by size:
5366 407 333
SacII CC_GC'GG
Cuts at: 140 167 432 2571 2935 140
Size: 27 265 2139 364 3311
Fragments arranged by size:
3311 2139 364 265 27
SalI G'TCGA_C
Cuts at: 3141 3141
Size: 6106
SanDI GG'GwC_CC
Cuts at: 169 1702 169
Size: 1533 4573
SapI GCTCTTCn'nnn_
Cuts at: 4127 4337 4127
Size: 210 5896
SbfI CC_TGCA'GG
Cuts at: 1061 3151 1061
Size: 2090 4016
ScaI AGT'ACT
Cuts at: 5623 5623
Size: 6106
SexAI A'CCwGG_T
Cuts at: 665 665
Size: 6106
SfcI C'TryA_G
Cuts at: 33 724 1057 1279 1386 2149 2347 3147 3832
Size: 691 333 222 107 763 198 800 685
Cuts at: 3832 4827 5018 33
Size: 995 191 1121
Fragments arranged by size:
1121 995 800 763 691 685 333 222
198 191 107
SfoI GGC'GCC
Cuts at: 2795 2855 2993 3536 3784 2795
Size: 60 138 543 248 5117
Fragments arranged by size:
5117 543 248 138 60
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6106
SgrAI Cr'CCGG_yG
Cuts at: 856 856
Size: 6106
SmaI CCC'GGG
Cuts at: 298 415 1494 2974 3126 298
Size: 117 1079 1480 152 3278
Fragments arranged by size:
3278 1480 1079 152 117
SmlI C'TyrA_G
Cuts at: 3259 4174 4668 4930 3259
Size: 915 494 262 4435
Fragments arranged by size:
4435 915 494 262
SnaBI TAC'GTA
Cuts at: 3096 3096
Size: 6106
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6106
SphI G_CATG'C
Cuts at: 1032 3157 3657 1032
Size: 2125 500 3481
Fragments arranged by size:
3481 2125 500
StuI AGG'CCT
Cuts at: 1405 1405
Size: 6106
StyI C'CwwG_G
Cuts at: 125 1289 1929 1985 3238 3592 125
Size: 1164 640 56 1253 354 2639
Fragments arranged by size:
2639 1253 1164 640 354 56
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1060 1341 2830 3687 3820 4279 4490 1060
Size: 281 1489 857 133 459 211 2676
Fragments arranged by size:
2676 1489 857 459 281 211 133
TatI w'GTAC_w
Cuts at: 368 5621 368
Size: 5253 853
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5623 5655 5623
Size: 32 6074
Tth111I GACn'n_nGTC
Cuts at: 3901 3901
Size: 6106
XbaI T'CTAG_A
Cuts at: 3135 3135
Size: 6106
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3599 3599
Size: 6106
XmaI C'CCGG_G
Cuts at: 296 413 1492 2972 3124 296
Size: 117 1079 1480 152 3278
Fragments arranged by size:
3278 1480 1079 152 117
ZraI GAC'GTC
Cuts at: 1233 1233
Size: 6106
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FseI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NruI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PspOMI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AflII AhdI AscI AvrII BaeI BbsI BfrBI BmtI
BsiWI BsrGI BstZ17I ClaI EcoRV FspAI HindIII HpaI
MfeI NdeI NheI NotI NsiI PacI PshAI PsiI
PspXI PsrI SfiI SrfI SspI SwaI XhoI XmnI