Restriction map for
pF1KSDF0070
(Circular) (Six-base) MAPSORT of: pF1KSDF0070.seq Check: 5656 from: 1 to: 7045
pF1KSDF0070 7045 bp
With 182 enzymes: SgfI *
June 20, 2008 10:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 905 905
Size: 7045
AccI GT'mk_AC
Cuts at: 3005 4081 3005
Size: 1076 5969
Acc65I G'GTAC_C
Cuts at: 571 4059 571
Size: 3488 3557
AclI AA'CG_TT
Cuts at: 6885 6885
Size: 7045
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2764 4868 5300 6049 6317 2764
Size: 2104 432 749 268 3492
Fragments arranged by size:
3492 2104 749 432 268
AfeI AGC'GCT
Cuts at: 304 1447 3937 304
Size: 1143 2490 3412
Fragments arranged by size:
3412 2490 1143
AflIII A'CryG_T
Cuts at: 2501 3530 4395 5501 6478 2501
Size: 1029 865 1106 977 3068
Fragments arranged by size:
3068 1106 1029 977 865
AgeI A'CCGG_T
Cuts at: 1671 1937 5433 1671
Size: 266 3496 3283
Fragments arranged by size:
3496 3283 266
AleI CACnn'nnGTG
Cuts at: 1099 2840 2929 1099
Size: 1741 89 5215
Fragments arranged by size:
5215 1741 89
AlwNI CAG_nnn'CTG
Cuts at: 510 897 1117 1846 2381 3032 3348 4348 5917
Size: 387 220 729 535 651 316 1000 1569
Cuts at: 5917 510
Size: 1638
Fragments arranged by size:
1638 1569 1000 729 651 535 387 316
220
ApaI G_GGCC'C
Cuts at: 137 612 713 1903 3470 137
Size: 475 101 1190 1567 3712
Fragments arranged by size:
3712 1567 1190 475 101
ApaLI G'TGCA_C
Cuts at: 847 5815 847
Size: 4968 2077
ApoI r'AATT_y
Cuts at: 4047 6398 6654 4047
Size: 2351 256 4438
Fragments arranged by size:
4438 2351 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 7045
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7045
AvaI C'yCGr_G
Cuts at: 130 137 712 762 829 1741 2001 2287 2409
Size: 7 575 50 67 912 260 286 122
Cuts at: 2409 2742 3463 3493 4063 130
Size: 333 721 30 570 3112
Fragments arranged by size:
3112 912 721 575 570 333 286 260
122 67 50 30 7
AvrII C'CTAG_G
Cuts at: 756 756
Size: 7045
BamHI G'GATC_C
Cuts at: 4068 4068
Size: 7045
BanI G'GyrC_C
Cuts at: 546 571 750 1462 2077 3459 3634 3823 4059
Size: 25 179 712 615 1382 175 189 236
Cuts at: 4059 4473 4721 4756 546
Size: 414 248 35 2835
Fragments arranged by size:
2835 1382 712 615 414 248 236 189
179 175 35 25
BanII G_rGCy'C
Cuts at: 137 385 439 612 713 865 1354 1612 1903
Size: 248 54 173 101 152 489 258 291
Cuts at: 1903 1994 2416 2788 3355 3430 3470 3502 4057
Size: 91 422 372 567 75 40 32 555
Cuts at: 4057 5087 137
Size: 1030 2095
Fragments arranged by size:
2095 1030 567 555 489 422 372 291
258 248 173 152 101 91 75 54
40 32
BbeI G_GCGC'C
Cuts at: 2081 3638 3827 4477 4725 2081
Size: 1557 189 650 248 4401
Fragments arranged by size:
4401 1557 650 248 189
BbsI GAAGACnn'nnnn_
Cuts at: 1031 2234 1031
Size: 1203 5842
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 488 522 3102 3136 488
Size: 34 2580 34 4397
Fragments arranged by size:
4397 2580 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 1495 4941 5704 7045 1495
Size: 3446 763 1341 1495
Fragments arranged by size:
3446 1495 1341 763
BclI T'GATC_A
Cuts at: 4563 4563
Size: 7045
BglI GCCn_nnn'nGGC
Cuts at: 236 1271 2267 2336 2561 3404 3620 236
Size: 1035 996 69 225 843 216 3661
Fragments arranged by size:
3661 1035 996 843 225 216 69
BglII A'GATC_T
Cuts at: 770 1481 4558 6597 770
Size: 711 3077 2039 1218
Fragments arranged by size:
3077 2039 1218 711
BlpI GC'TnA_GC
Cuts at: 3012 4155 3012
Size: 1143 5902
Bme1580I G_kGCm'C
Cuts at: 137 463 551 612 713 755 851 1903 2436
Size: 326 88 61 101 42 96 1052 533
Cuts at: 2436 3464 3470 3516 3655 4668 4761 5819 137
Size: 1028 6 46 139 1013 93 1058 1363
Fragments arranged by size:
1363 1058 1052 1028 1013 533 326 139
101 96 93 88 61 46 42 6
BmgBI CAC'GTC
Cuts at: 2355 2502 3231 3369 3531 2355
Size: 147 729 138 162 5869
Fragments arranged by size:
5869 729 162 147 138
BmrI ACTGGGnnnn_n'
Cuts at: 539 2995 4255 4336 4671 6817 539
Size: 2456 1260 81 335 2146 767
Fragments arranged by size:
2456 2146 1260 767 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 669 1767 5410 669
Size: 1098 3643 2304
Fragments arranged by size:
3643 2304 1098
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3558 3558
Size: 7045
Bpu10I CC'TnA_GC
Cuts at: 582 595 1355 3349 582
Size: 13 760 1994 4278
Fragments arranged by size:
4278 1994 760 13
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 334 4219 5098 5592 5890 334
Size: 3885 879 494 298 1489
Fragments arranged by size:
3885 1489 879 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 6749 6749
Size: 7045
BsaAI yAC'GTr
Cuts at: 1123 2073 4035 4396 1123
Size: 950 1962 361 3772
Fragments arranged by size:
3772 1962 950 361
BsaBI GATnn'nnATC
Cuts at: 6 4557 6596 6
Size: 4551 2039 455
Fragments arranged by size:
4551 2039 455
BsaHI Gr'CG_yC
Cuts at: 2078 3635 3824 3860 4474 4722 5426 6922 2078
Size: 1557 189 36 614 248 704 1496 2201
Fragments arranged by size:
2201 1557 1496 704 614 248 189 36
BsaWI w'CCGG_w
Cuts at: 642 1317 1602 1671 1937 2582 4232 4753 5433
Size: 675 285 69 266 645 1650 521 680
Cuts at: 5433 5707 5854 6265 642
Size: 274 147 411 1422
Fragments arranged by size:
1650 1422 680 675 645 521 411 285
274 266 147 69
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1231 1261 1707 1737 1877 1907 3133 3163 1231
Size: 30 446 30 140 30 1226 30 5113
Fragments arranged by size:
5113 1226 446 140 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 483 572 1336 1696 2860 3781 483
Size: 89 764 360 1164 921 3747
Fragments arranged by size:
3747 1164 921 764 360 89
BseYI C'CCAG_C
Cuts at: 225 731 890 1082 1488 1492 1592 1949 2494
Size: 506 159 192 406 4 100 357 545
Cuts at: 2494 2936 2969 3087 3629 4019 4468 5226 5805
Size: 442 33 118 542 390 449 758 579
Cuts at: 5805 6301 225
Size: 496 969
Fragments arranged by size:
969 758 579 545 542 506 496 449
442 406 390 357 192 159 118 100
33 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 805 1123 1552 1714 2053 2409 2751 3291 805
Size: 318 429 162 339 356 342 540 4559
Fragments arranged by size:
4559 540 429 356 342 339 318 162
BsiEI CG_ry'CG
Cuts at: 104 260 931 2463 3477 3506 3649 3817 4585
Size: 156 671 1532 1014 29 143 168 768
Cuts at: 4585 4631 5841 104
Size: 46 1210 1308
Fragments arranged by size:
1532 1308 1210 1014 768 671 168 156
143 46 29
BsiHKAI G_wGCw'C
Cuts at: 493 851 865 1361 4057 4835 5025 5819 493
Size: 358 14 496 2696 778 190 794 1719
Fragments arranged by size:
2696 1719 794 778 496 358 190 14
BsmI GAATG_Cn'
Cuts at: 2116 3226 2116
Size: 1110 5935
Bsp1286I G_dGCh'C
Cuts at: 137 385 439 463 493 551 612 713 755
Size: 248 54 24 30 58 61 101 42
Cuts at: 755 851 865 1354 1361 1612 1903 1994 2416
Size: 96 14 489 7 251 291 91 422
Cuts at: 2416 2436 2788 3355 3430 3464 3470 3502 3516
Size: 20 352 567 75 34 6 32 14
Cuts at: 3516 3655 4057 4668 4761 4835 5025 5087 5819
Size: 139 402 611 93 74 190 62 732
Cuts at: 5819 137
Size: 1363
Fragments arranged by size:
1363 732 611 567 489 422 402 352
291 251 248 190 139 101 96 93
91 75 74 62 61 58 54 42
34 32 30 24 20 14 14 7
6
BspEI T'CCGG_A
Cuts at: 642 1602 6265 642
Size: 960 4663 1422
Fragments arranged by size:
4663 1422 960
BspHI T'CATG_A
Cuts at: 6221 6221
Size: 7045
BspMI ACCTGCnnnn'nnnn_
Cuts at: 905 3221 3428 4093 4307 4609 4990 905
Size: 2316 207 665 214 302 381 2960
Fragments arranged by size:
2960 2316 665 381 302 214 207
BsrBI CCG'CTC
Cuts at: 50 267 3522 5335 5389 6875 7040 50
Size: 217 3255 1813 54 1486 165 55
Fragments arranged by size:
3255 1813 1486 217 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1208 1304 3675 4955 1208
Size: 96 2371 1280 3298
Fragments arranged by size:
3298 2371 1280 96
BsrFI r'CCGG_y
Cuts at: 255 259 324 562 574 1671 1937 2026 2195
Size: 4 65 238 12 1097 266 89 169
Cuts at: 2195 2965 3979 5041 5222 5433 255
Size: 770 1014 1062 181 211 1867
Fragments arranged by size:
1867 1097 1062 1014 770 266 238 211
181 169 89 65 12 4
BssHII G'CGCG_C
Cuts at: 1433 3988 1433
Size: 2555 4490
BssSI C'ACGA_G
Cuts at: 2091 5314 5674 2091
Size: 3223 360 3462
Fragments arranged by size:
3462 3223 360
BstAPI GCAn_nnn'nTGC
Cuts at: 900 1178 2381 2711 4420 900
Size: 278 1203 330 1709 3525
Fragments arranged by size:
3525 1709 1203 330 278
BstBI TT'CG_AA
Cuts at: 110 110
Size: 7045
BstEII G'GTnAC_C
Cuts at: 1656 1656
Size: 7045
BstYI r'GATC_y
Cuts at: 1 770 1481 2536 4068 4535 4558 4893 5139
Size: 769 711 1055 1532 467 23 335 246
Cuts at: 5139 6153 6239 6251 6597 1
Size: 1014 86 12 346 449
Fragments arranged by size:
1532 1055 1014 769 711 467 449 346
335 246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1631 1631
Size: 7045
BtgI C'CryG_G
Cuts at: 800 1221 1661 1881 2055 2760 3624 3811 800
Size: 421 440 220 174 705 864 187 4034
Fragments arranged by size:
4034 864 705 440 421 220 187 174
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2289 2819 3671 4993 5174 5337 2289
Size: 530 852 1322 181 163 3997
Fragments arranged by size:
3997 1322 852 530 181 163
BtsI GCAGTG_nn'
Cuts at: 1046 1176 1419 1920 2046 2589 2979 4286 1046
Size: 130 243 501 126 543 390 1307 3805
Fragments arranged by size:
3805 1307 543 501 390 243 130 126
DraI TTT'AAA
Cuts at: 4042 4042
Size: 7045
DrdI GACnn_nn'nnGTC
Cuts at: 4749 5609 4749
Size: 860 6185
EaeI y'GGCC_r
Cuts at: 257 411 483 659 928 1272 1878 2460 2921
Size: 154 72 176 269 344 606 582 461
Cuts at: 2921 3405 3474 3503 3578 3798 3814 4628 4802
Size: 484 69 29 75 220 16 814 174
Cuts at: 4802 5193 5220 6567 257
Size: 391 27 1347 735
Fragments arranged by size:
1347 814 735 606 582 484 461 391
344 269 220 176 174 154 75 72
69 29 27 16
EagI C'GGCC_G
Cuts at: 257 928 2460 3474 3503 3814 4628 257
Size: 671 1532 1014 29 311 814 2674
Fragments arranged by size:
2674 1532 1014 814 671 311 29
EarI CTCTTCn'nnn_
Cuts at: 1321 1640 3353 5066 5276 1321
Size: 319 1713 1713 210 3090
Fragments arranged by size:
3090 1713 1713 319 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1330 5251 5563 5709 6855 1330
Size: 3921 312 146 1146 1520
Fragments arranged by size:
3921 1520 1146 312 146
EcoICRI GAG'CTC
Cuts at: 863 4055 863
Size: 3192 3853
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 2764 3558 4868 5300 6049 6317 2764
Size: 794 1310 432 749 268 3492
Fragments arranged by size:
3492 1310 794 749 432 268
EcoO109I rG'GnC_Cy
Cuts at: 134 586 608 609 721 789 1114 1285 1900
Size: 452 22 1 112 68 325 171 615
Cuts at: 1900 2247 2342 2739 2765 3384 3467 3671 4007
Size: 347 95 397 26 619 83 204 336
Cuts at: 4007 4182 134
Size: 175 2997
Fragments arranged by size:
2997 619 615 452 397 347 336 325
204 175 171 112 95 83 68 26
22 1
EcoRI G'AATT_C
Cuts at: 4047 4047
Size: 7045
EcoRV GAT'ATC
Cuts at: 3885 3885
Size: 7045
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 333 3210 5062 333
Size: 2877 1852 2316
Fragments arranged by size:
2877 2316 1852
FseI GG_CCGG'CC
Cuts at: 259 259
Size: 7045
FspI TGC'GCA
Cuts at: 1200 4824 1200
Size: 3624 3421
HaeII r_GCGC'y
Cuts at: 202 292 306 537 628 1139 1449 1814 2081
Size: 90 14 231 91 511 310 365 267
Cuts at: 2081 2091 3342 3638 3827 3939 4026 4477 4725
Size: 10 1251 296 189 112 87 451 248
Cuts at: 4725 5749 6728 202
Size: 1024 979 519
Fragments arranged by size:
1251 1024 979 519 511 451 365 310
296 267 248 231 189 112 91 90
87 14 10
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1672 1704 1740 1772 2239 2271 4885 4915 4917
Size: 32 36 32 467 32 2614 30 2
Cuts at: 4917 4947 1672
Size: 30 3770
Fragments arranged by size:
3770 2614 467 36 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 3075 3417 4082 3075
Size: 342 665 6038
Fragments arranged by size:
6038 665 342
KasI G'GCGC_C
Cuts at: 2077 3634 3823 4473 4721 2077
Size: 1557 189 650 248 4401
Fragments arranged by size:
4401 1557 650 248 189
KpnI G_GTAC'C
Cuts at: 575 4063 575
Size: 3488 3557
MfeI C'AATT_G
Cuts at: 2040 2040
Size: 7045
MluI A'CGCG_T
Cuts at: 6478 6478
Size: 7045
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 410 3107 3278 5716 5900 410
Size: 2697 171 2438 184 1555
Fragments arranged by size:
2697 2438 1555 184 171
MscI TGG'CCA
Cuts at: 661 1274 1880 2923 4804 661
Size: 613 606 1043 1881 2902
Fragments arranged by size:
2902 1881 1043 613 606
MslI CAynn'nnrTG
Cuts at: 1099 2017 2840 2929 5032 5159 1099
Size: 918 823 89 2103 127 2985
Fragments arranged by size:
2985 2103 918 823 127 89
MspA1I CmG'CkG
Cuts at: 956 1086 1468 1492 2057 2423 3299 3410 3437
Size: 130 382 24 565 366 876 111 27
Cuts at: 3437 3626 3803 3813 3875 3953 3965 4154 4468
Size: 189 177 10 62 78 12 189 314
Cuts at: 4468 4828 5843 6088 956
Size: 360 1015 245 1913
Fragments arranged by size:
1913 1015 876 565 382 366 360 314
245 189 189 177 130 111 78 62
27 24 12 10
NaeI GCC'GGC
Cuts at: 257 261 2967 3981 5224 257
Size: 4 2706 1014 1243 2078
Fragments arranged by size:
2706 2078 1243 1014 4
NarI GG'CG_CC
Cuts at: 2078 3635 3824 4474 4722 2078
Size: 1557 189 650 248 4401
Fragments arranged by size:
4401 1557 650 248 189
NcoI C'CATG_G
Cuts at: 1221 2760 1221
Size: 1539 5506
NgoMIV G'CCGG_C
Cuts at: 255 259 2965 3979 5222 255
Size: 4 2706 1014 1243 2078
Fragments arranged by size:
2706 2078 1243 1014 4
NruI TCG'CGA
Cuts at: 498 2351 5406 498
Size: 1853 3055 2137
Fragments arranged by size:
3055 2137 1853
NspI r_CATG'y
Cuts at: 3117 3564 4096 4596 5505 3117
Size: 447 532 500 909 4657
Fragments arranged by size:
4657 909 532 500 447
PasI CC'CwG_GG
Cuts at: 948 1149 2436 2682 3455 948
Size: 201 1287 246 773 4538
Fragments arranged by size:
4538 1287 773 246 201
PciI A'CATG_T
Cuts at: 5501 5501
Size: 7045
PflMI CCAn_nnn'nTGG
Cuts at: 1459 1778 1459
Size: 319 6726
PmeI GTTT'AAAC
Cuts at: 4042 4042
Size: 7045
PmlI CAC'GTG
Cuts at: 2073 2073
Size: 7045
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6184 6216 6184
Size: 32 7013
PpuMI rG'GwC_Cy
Cuts at: 2247 2739 3384 3671 2247
Size: 492 645 287 5621
Fragments arranged by size:
5621 645 492 287
PshAI GACnn'nnGTC
Cuts at: 371 3782 371
Size: 3411 3634
PspOMI G'GGCC_C
Cuts at: 133 608 709 1899 3466 133
Size: 475 101 1190 1567 3712
Fragments arranged by size:
3712 1567 1190 475 101
PstI C_TGCA'G
Cuts at: 1091 1414 2524 2625 4090 4775 1091
Size: 323 1110 101 1465 685 3361
Fragments arranged by size:
3361 1465 1110 685 323 101
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7045
PvuII CAG'CTG
Cuts at: 1086 1492 4468 4828 1086
Size: 406 2976 360 3303
Fragments arranged by size:
3303 2976 406 360
RsrII CG'GwC_CG
Cuts at: 5238 5238
Size: 7045
SacI G_AGCT'C
Cuts at: 865 4057 865
Size: 3192 3853
SacII CC_GC'GG
Cuts at: 2058 3627 3814 2058
Size: 1569 187 5289
Fragments arranged by size:
5289 1569 187
SalI G'TCGA_C
Cuts at: 4080 4080
Size: 7045
SanDI GG'GwC_CC
Cuts at: 2247 3671 2247
Size: 1424 5621
SapI GCTCTTCn'nnn_
Cuts at: 1321 5066 5276 1321
Size: 3745 210 3090
Fragments arranged by size:
3745 3090 210
SbfI CC_TGCA'GG
Cuts at: 4090 4090
Size: 7045
ScaI AGT'ACT
Cuts at: 6562 6562
Size: 7045
SfcI C'TryA_G
Cuts at: 33 1087 1410 2520 2621 2664 3006 4086 4771
Size: 1054 323 1110 101 43 342 1080 685
Cuts at: 4771 5766 5957 33
Size: 995 191 1121
Fragments arranged by size:
1121 1110 1080 1054 995 685 342 323
191 101 43
SfoI GGC'GCC
Cuts at: 2079 3636 3825 4475 4723 2079
Size: 1557 189 650 248 4401
Fragments arranged by size:
4401 1557 650 248 189
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7045
SgrAI Cr'CCGG_yG
Cuts at: 324 1671 2026 324
Size: 1347 355 5343
Fragments arranged by size:
5343 1347 355
SmaI CCC'GGG
Cuts at: 132 714 764 2003 2289 2411 3465 4065 132
Size: 582 50 1239 286 122 1054 600 3112
Fragments arranged by size:
3112 1239 1054 600 582 286 122 50
SmlI C'TyrA_G
Cuts at: 349 4198 5113 5607 5869 349
Size: 3849 915 494 262 1525
Fragments arranged by size:
3849 1525 915 494 262
SnaBI TAC'GTA
Cuts at: 4035 4035
Size: 7045
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7045
SphI G_CATG'C
Cuts at: 4096 4596 4096
Size: 500 6545
SrfI GCCC'GGGC
Cuts at: 132 3465 132
Size: 3333 3712
StuI AGG'CCT
Cuts at: 835 1426 835
Size: 591 6454
StyI C'CwwG_G
Cuts at: 756 1221 2760 4177 4531 756
Size: 465 1539 1417 354 3270
Fragments arranged by size:
3270 1539 1417 465 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 952 1189 1388 1599 1721 2689 2839 3070 3447
Size: 237 199 211 122 968 150 231 377
Cuts at: 3447 4626 4759 5218 5429 952
Size: 1179 133 459 211 2568
Fragments arranged by size:
2568 1179 968 459 377 237 231 211
211 199 150 133 122
TatI w'GTAC_w
Cuts at: 1091 3323 6560 1091
Size: 2232 3237 1576
Fragments arranged by size:
3237 2232 1576
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1707 1739 1877 1909 3133 3165 3547 3579 6562
Size: 32 138 32 1224 32 382 32 2983
Cuts at: 6562 6594 1707
Size: 32 2158
Fragments arranged by size:
2983 2158 1224 382 138 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 4840 4840
Size: 7045
XbaI T'CTAG_A
Cuts at: 4074 4074
Size: 7045
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1873 4538 1873
Size: 2665 4380
XmaI C'CCGG_G
Cuts at: 130 712 762 2001 2287 2409 3463 4063 130
Size: 582 50 1239 286 122 1054 600 3112
Fragments arranged by size:
3112 1239 1054 600 582 286 122 50
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
AvrII BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI
BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI EcoRV FalI FseI FspI HaeII Hin4I
HincII KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NruI NspI PasI
PciI PflMI PmeI PmlI PpiI PpuMI PshAI PspOMI
PstI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AflII AhdI AlfI AloI AscI BaeI BbvCI
BfrBI BmtI BsiWI BsmBI BsrGI BstXI BstZ17I ClaI
CspCI DraIII EcoNI FspAI HindIII HpaI NdeI NheI
NotI NsiI PacI PfoI PsiI PspXI PsrI SexAI
SfiI SspI SwaI XhoI XmnI ZraI