Restriction map for
pF1KSDF0106
(Circular) (Six-base) MAPSORT of: pF1KSDF0106.seq Check: 1341 from: 1 to: 4237
pF1KSDF0106 4237 bp
With 182 enzymes: SgfI *
June 20, 2008 10:36 ..
AccI GT'mk_AC
Cuts at: 1273 1273
Size: 4237
Acc65I G'GTAC_C
Cuts at: 1037 1251 1037
Size: 214 4023
AclI AA'CG_TT
Cuts at: 4077 4077
Size: 4237
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1105 2060 2492 3241 3509 1105
Size: 955 432 749 268 1833
Fragments arranged by size:
1833 955 749 432 268
AflII C'TTAA_G
Cuts at: 477 477
Size: 4237
AflIII A'CryG_T
Cuts at: 1587 2693 3670 1587
Size: 1106 977 2154
Fragments arranged by size:
2154 1106 977
AgeI A'CCGG_T
Cuts at: 368 2625 368
Size: 2257 1980
AhdI GACnn_n'nnGTC
Cuts at: 633 633
Size: 4237
AleI CACnn'nnGTG
Cuts at: 980 980
Size: 4237
AlwNI CAG_nnn'CTG
Cuts at: 597 1540 3109 597
Size: 943 1569 1725
Fragments arranged by size:
1725 1569 943
ApaI G_GGCC'C
Cuts at: 724 954 724
Size: 230 4007
ApaLI G'TGCA_C
Cuts at: 3007 3007
Size: 4237
ApoI r'AATT_y
Cuts at: 1239 3590 3846 1239
Size: 2351 256 1630
Fragments arranged by size:
2351 1630 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4237
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4237
AvaI C'yCGr_G
Cuts at: 513 609 717 1255 513
Size: 96 108 538 3495
Fragments arranged by size:
3495 538 108 96
BamHI G'GATC_C
Cuts at: 1260 1260
Size: 4237
BanI G'GyrC_C
Cuts at: 1037 1171 1251 1665 1913 1948 1037
Size: 134 80 414 248 35 3326
Fragments arranged by size:
3326 414 248 134 80 35
BanII G_rGCy'C
Cuts at: 343 445 724 925 954 1249 2279 343
Size: 102 279 201 29 295 1030 2301
Fragments arranged by size:
2301 1030 295 279 201 102 29
BbeI G_GCGC'C
Cuts at: 1175 1669 1917 1175
Size: 494 248 3495
Fragments arranged by size:
3495 494 248
BbsI GAAGACnn'nnnn_
Cuts at: 783 1201 783
Size: 418 3819
BbvCI CC'TCA_GC
Cuts at: 508 878 508
Size: 370 3867
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 793 827 793
Size: 34 4203
BciVI GTATCCnnnnn_n'
Cuts at: 2133 2896 4237 2133
Size: 763 1341 2133
Fragments arranged by size:
2133 1341 763
BclI T'GATC_A
Cuts at: 1755 1755
Size: 4237
BglI GCCn_nnn'nGGC
Cuts at: 354 683 874 949 1049 354
Size: 329 191 75 100 3542
Fragments arranged by size:
3542 329 191 100 75
BglII A'GATC_T
Cuts at: 1750 3789 1750
Size: 2039 2198
BlpI GC'TnA_GC
Cuts at: 1347 1347
Size: 4237
Bme1580I G_kGCm'C
Cuts at: 724 954 1100 1860 1953 3011 724
Size: 230 146 760 93 1058 1950
Fragments arranged by size:
1950 1058 760 230 146 93
BmrI ACTGGGnnnn_n'
Cuts at: 921 1447 1528 1863 4009 921
Size: 526 81 335 2146 1149
Fragments arranged by size:
2146 1149 526 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2602 2602
Size: 4237
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 281 371 477 584 717 892 930 281
Size: 90 106 107 133 175 38 3588
Fragments arranged by size:
3588 175 133 107 106 90 38
Bpu10I CC'TnA_GC
Cuts at: 508 878 508
Size: 370 3867
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1411 2290 2784 3082 1411
Size: 879 494 298 2566
Fragments arranged by size:
2566 879 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 377 1197 3941 377
Size: 820 2744 673
Fragments arranged by size:
2744 820 673
BsaAI yAC'GTr
Cuts at: 1227 1588 1227
Size: 361 3876
BsaBI GATnn'nnATC
Cuts at: 6 1749 3788 6
Size: 1743 2039 455
Fragments arranged by size:
2039 1743 455
BsaHI Gr'CG_yC
Cuts at: 1172 1666 1914 2618 4114 1172
Size: 494 248 704 1496 1295
Fragments arranged by size:
1496 1295 704 494 248
BsaWI w'CCGG_w
Cuts at: 368 1424 1945 2625 2899 3046 3457 368
Size: 1056 521 680 274 147 411 1148
Fragments arranged by size:
1148 1056 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1021 1051 1021
Size: 30 4207
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 757 895 757
Size: 138 4099
BseYI C'CCAG_C
Cuts at: 739 910 1660 2418 2997 3493 739
Size: 171 750 758 579 496 1483
Fragments arranged by size:
1483 758 750 579 496 171
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1011 1011
Size: 4237
BsiEI CG_ry'CG
Cuts at: 104 678 1777 1823 3033 104
Size: 574 1099 46 1210 1308
Fragments arranged by size:
1308 1210 1099 574 46
BsiHKAI G_wGCw'C
Cuts at: 1249 2027 2217 3011 1249
Size: 778 190 794 2475
Fragments arranged by size:
2475 794 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 389 389
Size: 4237
Bsp1286I G_dGCh'C
Cuts at: 343 445 724 925 954 1100 1249 1860 1953
Size: 102 279 201 29 146 149 611 93
Cuts at: 1953 2027 2217 2279 3011 343
Size: 74 190 62 732 1569
Fragments arranged by size:
1569 732 611 279 201 190 149 146
102 93 74 62 29
BspEI T'CCGG_A
Cuts at: 3457 3457
Size: 4237
BspHI T'CATG_A
Cuts at: 3413 3413
Size: 4237
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1285 1499 1801 2182 1285
Size: 214 302 381 3340
Fragments arranged by size:
3340 381 302 214
BsrBI CCG'CTC
Cuts at: 50 228 2527 2581 4067 4232 50
Size: 178 2299 54 1486 165 55
Fragments arranged by size:
2299 1486 178 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2147 2147
Size: 4237
BsrFI r'CCGG_y
Cuts at: 368 520 667 1034 2233 2414 2625 368
Size: 152 147 367 1199 181 211 1980
Fragments arranged by size:
1980 1199 367 211 181 152 147
BssSI C'ACGA_G
Cuts at: 2506 2866 2506
Size: 360 3877
BstAPI GCAn_nnn'nTGC
Cuts at: 1612 1612
Size: 4237
BstBI TT'CG_AA
Cuts at: 110 110
Size: 4237
BstXI CCAn_nnnn'nTGG
Cuts at: 161 954 161
Size: 793 3444
BstYI r'GATC_y
Cuts at: 1 329 1260 1727 1750 2085 2331 3345 3431
Size: 328 931 467 23 335 246 1014 86
Cuts at: 3431 3443 3789 1
Size: 12 346 449
Fragments arranged by size:
1014 931 467 449 346 335 328 246
86 23 12
BtgI C'CryG_G
Cuts at: 125 125
Size: 4237
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 987 2185 2366 2529 987
Size: 1198 181 163 2695
Fragments arranged by size:
2695 1198 181 163
BtsI GCAGTG_nn'
Cuts at: 1478 1478
Size: 4237
DraI TTT'AAA
Cuts at: 1234 1234
Size: 4237
DrdI GACnn_nn'nnGTC
Cuts at: 787 1941 2801 787
Size: 1154 860 2223
Fragments arranged by size:
2223 1154 860
EaeI y'GGCC_r
Cuts at: 146 221 275 572 675 956 1820 1994 2385
Size: 75 54 297 103 281 864 174 391
Cuts at: 2385 2412 3759 146
Size: 27 1347 624
Fragments arranged by size:
1347 864 624 391 297 281 174 103
75 54 27
EagI C'GGCC_G
Cuts at: 675 1820 675
Size: 1145 3092
EarI CTCTTCn'nnn_
Cuts at: 2258 2468 2258
Size: 210 4027
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 322 2443 2755 2901 4047 322
Size: 2121 312 146 1146 512
Fragments arranged by size:
2121 1146 512 312 146
EcoICRI GAG'CTC
Cuts at: 1247 1247
Size: 4237
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 281 371 477 584 717 892 930 1105 2060
Size: 90 106 107 133 175 38 175 955
Cuts at: 2060 2492 3241 3509 281
Size: 432 749 268 1009
Fragments arranged by size:
1009 955 749 432 268 175 175 133
107 106 90 38
EcoNI CCTnn'n_nnAGG
Cuts at: 477 775 477
Size: 298 3939
EcoO109I rG'GnC_Cy
Cuts at: 482 721 950 951 1374 482
Size: 239 229 1 423 3345
Fragments arranged by size:
3345 423 239 229 1
EcoRI G'AATT_C
Cuts at: 1239 1239
Size: 4237
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2254 2254
Size: 4237
FseI GG_CCGG'CC
Cuts at: 671 671
Size: 4237
FspI TGC'GCA
Cuts at: 1021 2016 1021
Size: 995 3242
FspAI rTGC'GCAy
Cuts at: 1021 1021
Size: 4237
HaeII r_GCGC'y
Cuts at: 160 1175 1669 1917 2941 3920 160
Size: 1015 494 248 1024 979 477
Fragments arranged by size:
1024 1015 979 494 477 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 254 286 454 486 619 651 766 798 2077
Size: 32 168 32 133 32 115 32 1279
Cuts at: 2077 2107 2109 2139 254
Size: 30 2 30 2352
Fragments arranged by size:
2352 1279 168 133 115 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 134 1274 134
Size: 1140 3097
HindIII A'AGCT_T
Cuts at: 1148 1148
Size: 4237
KasI G'GCGC_C
Cuts at: 1171 1665 1913 1171
Size: 494 248 3495
Fragments arranged by size:
3495 494 248
KpnI G_GTAC'C
Cuts at: 1041 1255 1041
Size: 214 4023
MluI A'CGCG_T
Cuts at: 3670 3670
Size: 4237
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2908 3092 2908
Size: 184 4053
MscI TGG'CCA
Cuts at: 277 958 1996 277
Size: 681 1038 2518
Fragments arranged by size:
2518 1038 681
MslI CAynn'nnrTG
Cuts at: 208 271 980 2224 2351 208
Size: 63 709 1244 127 2094
Fragments arranged by size:
2094 1244 709 127 63
MspA1I CmG'CkG
Cuts at: 363 622 1346 1660 2020 3035 3280 363
Size: 259 724 314 360 1015 245 1320
Fragments arranged by size:
1320 1015 724 360 314 259 245
NaeI GCC'GGC
Cuts at: 522 669 2416 522
Size: 147 1747 2343
Fragments arranged by size:
2343 1747 147
NarI GG'CG_CC
Cuts at: 1172 1666 1914 1172
Size: 494 248 3495
Fragments arranged by size:
3495 494 248
NcoI C'CATG_G
Cuts at: 125 125
Size: 4237
NgoMIV G'CCGG_C
Cuts at: 520 667 2414 520
Size: 147 1747 2343
Fragments arranged by size:
2343 1747 147
NruI TCG'CGA
Cuts at: 815 1006 2598 815
Size: 191 1592 2454
Fragments arranged by size:
2454 1592 191
NspI r_CATG'y
Cuts at: 306 313 605 1288 1788 2697 306
Size: 7 292 683 500 909 1846
Fragments arranged by size:
1846 909 683 500 292 7
PasI CC'CwG_GG
Cuts at: 161 161
Size: 4237
PciI A'CATG_T
Cuts at: 2693 2693
Size: 4237
PflMI CCAn_nnn'nTGG
Cuts at: 597 597
Size: 4237
PfoI T'CCnGG_A
Cuts at: 770 1025 770
Size: 255 3982
PmeI GTTT'AAAC
Cuts at: 1234 1234
Size: 4237
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3376 3408 3376
Size: 32 4205
PpuMI rG'GwC_Cy
Cuts at: 482 482
Size: 4237
PspOMI G'GGCC_C
Cuts at: 720 950 720
Size: 230 4007
PstI C_TGCA'G
Cuts at: 362 806 1282 1967 362
Size: 444 476 685 2632
Fragments arranged by size:
2632 685 476 444
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4237
PvuII CAG'CTG
Cuts at: 363 1660 2020 363
Size: 1297 360 2580
Fragments arranged by size:
2580 1297 360
RsrII CG'GwC_CG
Cuts at: 2430 2430
Size: 4237
SacI G_AGCT'C
Cuts at: 1249 1249
Size: 4237
SalI G'TCGA_C
Cuts at: 1272 1272
Size: 4237
SapI GCTCTTCn'nnn_
Cuts at: 2258 2468 2258
Size: 210 4027
SbfI CC_TGCA'GG
Cuts at: 1282 1282
Size: 4237
ScaI AGT'ACT
Cuts at: 3754 3754
Size: 4237
SfcI C'TryA_G
Cuts at: 33 358 802 1278 1963 2958 3149 33
Size: 325 444 476 685 995 191 1121
Fragments arranged by size:
1121 995 685 476 444 325 191
SfoI GGC'GCC
Cuts at: 1173 1667 1915 1173
Size: 494 248 3495
Fragments arranged by size:
3495 494 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4237
SmaI CCC'GGG
Cuts at: 611 719 1257 611
Size: 108 538 3591
Fragments arranged by size:
3591 538 108
SmlI C'TyrA_G
Cuts at: 477 1390 2305 2799 3061 477
Size: 913 915 494 262 1653
Fragments arranged by size:
1653 915 913 494 262
SnaBI TAC'GTA
Cuts at: 1227 1227
Size: 4237
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4237
SphI G_CATG'C
Cuts at: 306 313 1288 1788 306
Size: 7 975 500 2755
Fragments arranged by size:
2755 975 500 7
StyI C'CwwG_G
Cuts at: 125 239 413 1369 1723 125
Size: 114 174 956 354 2639
Fragments arranged by size:
2639 956 354 174 114
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1818 1951 2410 2621 1818
Size: 133 459 211 3434
Fragments arranged by size:
3434 459 211 133
TatI w'GTAC_w
Cuts at: 3752 3752
Size: 4237
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3754 3786 3754
Size: 32 4205
Tth111I GACn'n_nGTC
Cuts at: 2032 2032
Size: 4237
XbaI T'CTAG_A
Cuts at: 1266 1266
Size: 4237
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 741 1730 741
Size: 989 3248
XmaI C'CCGG_G
Cuts at: 609 717 1255 609
Size: 108 538 3591
Fragments arranged by size:
3591 538 108
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI
AleI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FseI FspI FspAI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NruI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PspOMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SnaBI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AlfI AloI AscI AvrII BaeI
BfrBI BmgBI BmtI BsiWI BsmI BsrGI BssHII BstEII
BstZ17I Bsu36I ClaI CspCI DraIII EcoRV HpaI MfeI
NdeI NheI NotI NsiI PacI PmlI PshAI PsiI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SrfI
SspI StuI SwaI XhoI XmnI ZraI