Restriction map for
pF1KSDF0128
(Circular) (Six-base) MAPSORT of: pF1KSDF0128.seq Check: 2013 from: 1 to: 7696
pF1KSDF0128 7696 bp
With 182 enzymes: SgfI *
September 4, 2008 14:04 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 305 438 2487 305
Size: 133 2049 5514
Fragments arranged by size:
5514 2049 133
AatII G_ACGT'C
Cuts at: 4496 4496
Size: 7696
AccI GT'mk_AC
Cuts at: 3269 4732 3269
Size: 1463 6233
Acc65I G'GTAC_C
Cuts at: 282 793 936 1234 4710 282
Size: 511 143 298 3476 3268
Fragments arranged by size:
3476 3268 511 298 143
AclI AA'CG_TT
Cuts at: 7536 7536
Size: 7696
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 923 1241 1523 1942 3544 3943 4064 5519 5951
Size: 318 282 419 1602 399 121 1455 432
Cuts at: 5951 6700 6968 923
Size: 749 268 1651
Fragments arranged by size:
1651 1602 1455 749 432 419 399 318
282 268 121
AfeI AGC'GCT
Cuts at: 2610 2752 2610
Size: 142 7554
AflIII A'CryG_T
Cuts at: 3294 5046 6152 7129 3294
Size: 1752 1106 977 3861
Fragments arranged by size:
3861 1752 1106 977
AgeI A'CCGG_T
Cuts at: 6084 6084
Size: 7696
AhdI GACnn_n'nnGTC
Cuts at: 2565 2565
Size: 7696
AleI CACnn'nnGTG
Cuts at: 489 567 3262 489
Size: 78 2695 4923
Fragments arranged by size:
4923 2695 78
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 317 3898 317
Size: 3581 4115
AlwNI CAG_nnn'CTG
Cuts at: 617 651 901 1090 1144 1453 1881 1992 2141
Size: 34 250 189 54 309 428 111 149
Cuts at: 2141 2481 2706 2809 3390 3747 4236 4999 6568
Size: 340 225 103 581 357 489 763 1569
Cuts at: 6568 617
Size: 1745
Fragments arranged by size:
1745 1569 763 581 489 428 357 340
309 250 225 189 149 111 103 54
34
ApaI G_GGCC'C
Cuts at: 2521 2739 2859 2898 3060 3071 3187 3831 4042
Size: 218 120 39 162 11 116 644 211
Cuts at: 4042 2521
Size: 6175
Fragments arranged by size:
6175 644 218 211 162 120 116 39
11
ApaLI G'TGCA_C
Cuts at: 685 4160 6466 685
Size: 3475 2306 1915
Fragments arranged by size:
3475 2306 1915
ApoI r'AATT_y
Cuts at: 4502 4698 7049 7305 4502
Size: 196 2351 256 4893
Fragments arranged by size:
4893 2351 256 196
AseI AT'TA_AT
Cuts at: 20 20
Size: 7696
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7696
AvaI C'yCGr_G
Cuts at: 1016 1817 2514 3512 3728 4379 4652 4714 1016
Size: 801 697 998 216 651 273 62 3998
Fragments arranged by size:
3998 998 801 697 651 273 216 62
BamHI G'GATC_C
Cuts at: 2978 4719 2978
Size: 1741 5955
BanI G'GyrC_C
Cuts at: 282 453 793 936 1234 1914 2875 3040 3343
Size: 171 340 143 298 680 961 165 303
Cuts at: 3343 3487 4710 5124 5372 5407 282
Size: 144 1223 414 248 35 2571
Fragments arranged by size:
2571 1223 961 680 414 340 303 298
248 171 165 144 143 35
BanII G_rGCy'C
Cuts at: 668 892 1133 1296 1330 1469 1770 1808 2203
Size: 224 241 163 34 139 301 38 395
Cuts at: 2203 2521 2739 2859 2867 2898 3010 3060 3071
Size: 318 218 120 8 31 112 50 11
Cuts at: 3071 3187 3703 3735 3788 3812 3831 4042 4268
Size: 116 516 32 53 24 19 211 226
Cuts at: 4268 4659 4708 5738 668
Size: 391 49 1030 2626
Fragments arranged by size:
2626 1030 516 395 391 318 301 241
226 224 218 211 163 139 120 116
112 53 50 49 38 34 32 31
24 19 11 8
BbeI G_GCGC'C
Cuts at: 5128 5376 5128
Size: 248 7448
BbvCI CC'TCA_GC
Cuts at: 459 1091 1493 2048 2612 3091 459
Size: 632 402 555 564 479 5064
Fragments arranged by size:
5064 632 564 555 479 402
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3965 3999 3965
Size: 34 7662
BciVI GTATCCnnnnn_n'
Cuts at: 188 5592 6355 7696 188
Size: 5404 763 1341 188
Fragments arranged by size:
5404 1341 763 188
BclI T'GATC_A
Cuts at: 1689 5214 1689
Size: 3525 4171
BglI GCCn_nnn'nGGC
Cuts at: 1043 1592 3014 3835 3895 4390 1043
Size: 549 1422 821 60 495 4349
Fragments arranged by size:
4349 1422 821 549 495 60
BglII A'GATC_T
Cuts at: 3918 5209 7248 3918
Size: 1291 2039 4366
Fragments arranged by size:
4366 2039 1291
BlpI GC'TnA_GC
Cuts at: 662 4806 662
Size: 4144 3552
Bme1580I G_kGCm'C
Cuts at: 330 458 536 689 818 1320 1795 2521 2739
Size: 128 78 153 129 502 475 726 218
Cuts at: 2739 2859 2898 3060 3071 3187 3381 3490 3513
Size: 120 39 162 11 116 194 109 23
Cuts at: 3513 3831 4042 4164 4353 5319 5412 6470 330
Size: 318 211 122 189 966 93 1058 1556
Fragments arranged by size:
1556 1058 966 726 502 475 318 218
211 194 189 162 153 129 128 122
120 116 109 93 78 39 23 11
BmgBI CAC'GTC
Cuts at: 3278 3278
Size: 7696
BmrI ACTGGGnnnn_n'
Cuts at: 1891 2129 2151 2861 3054 4906 4987 5322 7468
Size: 238 22 710 193 1852 81 335 2146
Cuts at: 7468 1891
Size: 2119
Fragments arranged by size:
2146 2119 1852 710 335 238 193 81
22
BmtI G_CTAG'C
Cuts at: 403 403
Size: 7696
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4616 4667 6061 4616
Size: 51 1394 6251
Fragments arranged by size:
6251 1394 51
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 737 906 977 1567 1645 1913 2248 2395 2476
Size: 169 71 590 78 268 335 147 81
Cuts at: 2476 2677 2728 3182 3265 3682 3799 4556 737
Size: 201 51 454 83 417 117 757 3877
Fragments arranged by size:
3877 757 590 454 417 335 268 201
169 147 117 83 81 78 71 51
Bpu10I CC'TnA_GC
Cuts at: 134 459 1091 1437 1493 1861 2048 2612 3091
Size: 325 632 346 56 368 187 564 479
Cuts at: 3091 3188 3464 3806 134
Size: 97 276 342 4024
Fragments arranged by size:
4024 632 564 479 368 346 342 325
276 187 97 56
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 472 1968 3456 3675 4018 4192 4870 5749 6243
Size: 1496 1488 219 343 174 678 879 494
Cuts at: 6243 6541 472
Size: 298 1627
Fragments arranged by size:
1627 1496 1488 879 678 494 343 298
219 174
BsaI GGTCTCn'nnnn_
Cuts at: 3872 3944 7400 3872
Size: 72 3456 4168
Fragments arranged by size:
4168 3456 72
BsaAI yAC'GTr
Cuts at: 2454 3297 3426 3642 4686 5047 2454
Size: 843 129 216 1044 361 5103
Fragments arranged by size:
5103 1044 843 361 216 129
BsaBI GATnn'nnATC
Cuts at: 6 5208 7247 6
Size: 5202 2039 455
Fragments arranged by size:
5202 2039 455
BsaHI Gr'CG_yC
Cuts at: 3472 4493 5125 5373 6077 7573 3472
Size: 1021 632 248 704 1496 3595
Fragments arranged by size:
3595 1496 1021 704 632 248
BsaWI w'CCGG_w
Cuts at: 929 974 1060 1159 3536 4883 5404 6084 6358
Size: 45 86 99 2377 1347 521 680 274
Cuts at: 6358 6505 6916 929
Size: 147 411 1709
Fragments arranged by size:
2377 1709 1347 680 521 411 274 147
99 86 45
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2086 2116 3836 3866 2086
Size: 30 1720 30 5916
Fragments arranged by size:
5916 1720 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 796 1208 1211 1214 1217 1220 1319 1816 2107
Size: 412 3 3 3 3 99 497 291
Cuts at: 2107 2236 2269 2542 2674 2988 3013 3796 4006
Size: 129 33 273 132 314 25 783 210
Cuts at: 4006 4486 4489 796
Size: 480 3 4003
Fragments arranged by size:
4003 783 497 480 412 314 291 273
210 132 129 99 33 25 3 3
3 3 3
BseYI C'CCAG_C
Cuts at: 763 789 1134 2196 2362 2388 2809 2919 3085
Size: 26 345 1062 166 26 421 110 166
Cuts at: 3085 3109 3303 3380 3799 4229 4567 5119 5877
Size: 24 194 77 419 430 338 552 758
Cuts at: 5877 6456 6952 763
Size: 579 496 1507
Fragments arranged by size:
1507 1062 758 579 552 496 430 421
419 345 338 194 166 166 110 77
26 26 24
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 175 415 538 1654 2434 2476 2822 3662 4294
Size: 240 123 1116 780 42 346 840 632
Cuts at: 4294 175
Size: 3577
Fragments arranged by size:
3577 1116 840 780 632 346 240 123
42
BsiEI CG_ry'CG
Cuts at: 104 5236 5282 6492 104
Size: 5132 46 1210 1308
Fragments arranged by size:
5132 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 163 668 689 1035 1394 1469 1770 3283 3788
Size: 505 21 346 359 75 301 1513 505
Cuts at: 3788 3812 4164 4268 4708 5486 5676 6470 163
Size: 24 352 104 440 778 190 794 1389
Fragments arranged by size:
1513 1389 794 778 505 505 440 359
352 346 301 190 104 75 24 21
BsmI GAATG_Cn'
Cuts at: 1411 2273 2670 1411
Size: 862 397 6437
Fragments arranged by size:
6437 862 397
BsmBI CGTCTCn'nnnn_
Cuts at: 1689 1689
Size: 7696
Bsp1286I G_dGCh'C
Cuts at: 163 330 458 536 668 689 818 892 1035
Size: 167 128 78 132 21 129 74 143
Cuts at: 1035 1133 1296 1320 1330 1394 1469 1770 1795
Size: 98 163 24 10 64 75 301 25
Cuts at: 1795 1808 2203 2521 2739 2859 2867 2898 3010
Size: 13 395 318 218 120 8 31 112
Cuts at: 3010 3060 3071 3187 3283 3381 3490 3513 3703
Size: 50 11 116 96 98 109 23 190
Cuts at: 3703 3735 3788 3812 3831 4042 4164 4268 4353
Size: 32 53 24 19 211 122 104 85
Cuts at: 4353 4659 4708 5319 5412 5486 5676 5738 6470
Size: 306 49 611 93 74 190 62 732
Cuts at: 6470 163
Size: 1389
Fragments arranged by size:
1389 732 611 395 318 306 301 218
211 190 190 167 163 143 132 129
128 122 120 116 112 109 104 98
98 96 93 85 78 75 74 74
64 62 53 50 49 32 31 25
24 24 23 21 19 13 11 10
8
BspEI T'CCGG_A
Cuts at: 929 1159 6916 929
Size: 230 5757 1709
Fragments arranged by size:
5757 1709 230
BspHI T'CATG_A
Cuts at: 2007 6872 2007
Size: 4865 2831
BspMI ACCTGCnnnn'nnnn_
Cuts at: 305 438 625 1273 2456 2487 2703 3731 3890
Size: 133 187 648 1183 31 216 1028 159
Cuts at: 3890 4744 4958 5260 5641 305
Size: 854 214 302 381 2360
Fragments arranged by size:
2360 1183 1028 854 648 381 302 216
214 187 159 133 31
BsrBI CCG'CTC
Cuts at: 50 369 4338 5986 6040 7526 7691 50
Size: 319 3969 1648 54 1486 165 55
Fragments arranged by size:
3969 1648 1486 319 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3141 5606 3141
Size: 2465 5231
BsrFI r'CCGG_y
Cuts at: 593 2872 4414 5692 5873 6084 593
Size: 2279 1542 1278 181 211 2205
Fragments arranged by size:
2279 2205 1542 1278 211 181
BsrGI T'GTAC_A
Cuts at: 3843 3843
Size: 7696
BssSI C'ACGA_G
Cuts at: 4163 5965 6325 4163
Size: 1802 360 5534
Fragments arranged by size:
5534 1802 360
BstAPI GCAn_nnn'nTGC
Cuts at: 423 453 2289 2481 2502 2545 3040 5071 423
Size: 30 1836 192 21 43 495 2031 3048
Fragments arranged by size:
3048 2031 1836 495 192 43 30 21
BstBI TT'CG_AA
Cuts at: 110 110
Size: 7696
BstEII G'GTnAC_C
Cuts at: 3249 3249
Size: 7696
BstXI CCAn_nnnn'nTGG
Cuts at: 210 337 210
Size: 127 7569
BstYI r'GATC_y
Cuts at: 1 1162 1971 2298 2978 3918 4485 4622 4719
Size: 1161 809 327 680 940 567 137 97
Cuts at: 4719 5186 5209 5544 5790 6804 6890 6902 7248
Size: 467 23 335 246 1014 86 12 346
Cuts at: 7248 1
Size: 449
Fragments arranged by size:
1161 1014 940 809 680 567 467 449
346 335 327 246 137 97 86 23
12
Bsu36I CC'TnA_GG
Cuts at: 779 839 1174 1799 3790 779
Size: 60 335 625 1991 4685
Fragments arranged by size:
4685 1991 625 335 60
BtgI C'CryG_G
Cuts at: 125 3010 3124 3286 3609 3704 3870 3890 4289
Size: 2885 114 162 323 95 166 20 399
Cuts at: 4289 4388 125
Size: 99 3433
Fragments arranged by size:
3433 2885 399 323 166 162 114 99
95 20
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 5644 5825 5988 5644
Size: 181 163 7352
Fragments arranged by size:
7352 181 163
BtsI GCAGTG_nn'
Cuts at: 724 1855 1921 2107 2267 2278 2314 2482 2512
Size: 1131 66 186 160 11 36 168 30
Cuts at: 2512 3054 4937 724
Size: 542 1883 3483
Fragments arranged by size:
3483 1883 1131 542 186 168 160 66
36 30 11
DraI TTT'AAA
Cuts at: 4693 4693
Size: 7696
DraIII CAC_nnn'GTG
Cuts at: 1387 1387
Size: 7696
DrdI GACnn_nn'nnGTC
Cuts at: 5400 6260 5400
Size: 860 6836
EaeI y'GGCC_r
Cuts at: 364 595 2081 3127 3428 3887 3972 4391 4610
Size: 231 1486 1046 301 459 85 419 219
Cuts at: 4610 5279 5453 5844 5871 7218 364
Size: 669 174 391 27 1347 842
Fragments arranged by size:
1486 1347 1046 842 669 459 419 391
301 231 219 174 85 27
EagI C'GGCC_G
Cuts at: 5279 5279
Size: 7696
EarI CTCTTCn'nnn_
Cuts at: 352 1517 1658 1844 2246 3173 4009 4359 4536
Size: 1165 141 186 402 927 836 350 177
Cuts at: 4536 5717 5927 352
Size: 1181 210 2121
Fragments arranged by size:
2121 1181 1165 927 836 402 350 210
186 177 141
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 5902 6214 6360 7506 5902
Size: 312 146 1146 6092
Fragments arranged by size:
6092 1146 312 146
EcoICRI GAG'CTC
Cuts at: 666 1467 1768 3786 3810 4266 4706 666
Size: 801 301 2018 24 456 440 3656
Fragments arranged by size:
3656 2018 801 456 440 301 24
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 737 906 923 977 1241 1523 1567 1645 1913
Size: 169 17 54 264 282 44 78 268
Cuts at: 1913 1942 2248 2395 2476 2677 2728 3182 3265
Size: 29 306 147 81 201 51 454 83
Cuts at: 3265 3544 3682 3799 3943 4064 4556 5519 5951
Size: 279 138 117 144 121 492 963 432
Cuts at: 5951 6700 6968 737
Size: 749 268 1465
Fragments arranged by size:
1465 963 749 492 454 432 306 282
279 268 268 264 201 169 147 144
138 121 117 83 81 78 54 51
44 29 17
EcoNI CCTnn'n_nnAGG
Cuts at: 830 1417 1909 2632 2995 830
Size: 587 492 723 363 5531
Fragments arranged by size:
5531 723 587 492 363
EcoO109I rG'GnC_Cy
Cuts at: 696 835 1005 1012 1594 1668 2124 2646 2736
Size: 139 170 7 582 74 456 522 90
Cuts at: 2736 2856 2895 3056 3067 3068 3183 3184 3215
Size: 120 39 161 11 1 115 1 31
Cuts at: 3215 3593 3828 4038 4039 4833 696
Size: 378 235 210 1 794 3559
Fragments arranged by size:
3559 794 582 522 456 378 235 210
170 161 139 120 115 90 74 39
31 11 7 1 1 1
EcoRI G'AATT_C
Cuts at: 4698 4698
Size: 7696
EcoRV GAT'ATC
Cuts at: 2691 2691
Size: 7696
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1381 1687 4095 5713 1381
Size: 306 2408 1618 3364
Fragments arranged by size:
3364 2408 1618 306
FspI TGC'GCA
Cuts at: 5475 5475
Size: 7696
HaeII r_GCGC'y
Cuts at: 790 956 2612 2628 2754 5128 5376 6400 7379
Size: 166 1656 16 126 2374 248 1024 979
Cuts at: 7379 790
Size: 1107
Fragments arranged by size:
2374 1656 1107 1024 979 248 166 126
16
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 228 260 470 502 1718 1750 2041 2073 2551
Size: 32 210 32 1216 32 291 32 478
Cuts at: 2551 2583 5536 5566 5568 5598 228
Size: 32 2953 30 2 30 2326
Fragments arranged by size:
2953 2326 1216 478 291 210 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 4733 4733
Size: 7696
HindIII A'AGCT_T
Cuts at: 1396 2541 1396
Size: 1145 6551
KasI G'GCGC_C
Cuts at: 5124 5372 5124
Size: 248 7448
KpnI G_GTAC'C
Cuts at: 286 797 940 1238 4714 286
Size: 511 143 298 3476 3268
Fragments arranged by size:
3476 3268 511 298 143
MluI A'CGCG_T
Cuts at: 7129 7129
Size: 7696
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 687 1636 2044 2468 6367 6551 687
Size: 949 408 424 3899 184 1832
Fragments arranged by size:
3899 1832 949 424 408 184
MscI TGG'CCA
Cuts at: 2083 3430 4612 5455 2083
Size: 1347 1182 843 4324
Fragments arranged by size:
4324 1347 1182 843
MslI CAynn'nnrTG
Cuts at: 489 567 1697 2341 2772 3262 5683 5810 489
Size: 78 1130 644 431 490 2421 127 2375
Fragments arranged by size:
2421 2375 1130 644 490 431 127 78
MspA1I CmG'CkG
Cuts at: 463 648 1623 1989 2472 2535 2703 2945 2957
Size: 185 975 366 483 63 168 242 12
Cuts at: 2957 3387 3756 3849 4124 4233 4805 5119 5479
Size: 430 369 93 275 109 572 314 360
Cuts at: 5479 6494 6739 463
Size: 1015 245 1420
Fragments arranged by size:
1420 1015 975 572 483 430 369 366
360 314 275 245 242 185 168 109
93 63 12
NaeI GCC'GGC
Cuts at: 2874 4416 5875 2874
Size: 1542 1459 4695
Fragments arranged by size:
4695 1542 1459
NarI GG'CG_CC
Cuts at: 5125 5373 5125
Size: 248 7448
NcoI C'CATG_G
Cuts at: 125 3704 4289 125
Size: 3579 585 3532
Fragments arranged by size:
3579 3532 585
NgoMIV G'CCGG_C
Cuts at: 2872 4414 5873 2872
Size: 1542 1459 4695
Fragments arranged by size:
4695 1542 1459
NheI G'CTAG_C
Cuts at: 399 399
Size: 7696
NruI TCG'CGA
Cuts at: 6057 6057
Size: 7696
NspI r_CATG'y
Cuts at: 4414 4747 5247 6156 4414
Size: 333 500 909 5954
Fragments arranged by size:
5954 909 500 333
PasI CC'CwG_GG
Cuts at: 502 656 1330 2521 3118 3332 3457 4588 502
Size: 154 674 1191 597 214 125 1131 3610
Fragments arranged by size:
3610 1191 1131 674 597 214 154 125
PciI A'CATG_T
Cuts at: 6152 6152
Size: 7696
PflMI CCAn_nnn'nTGG
Cuts at: 231 2141 2163 3457 231
Size: 1910 22 1294 4470
Fragments arranged by size:
4470 1910 1294 22
PfoI T'CCnGG_A
Cuts at: 2618 2882 3776 3854 4313 2618
Size: 264 894 78 459 6001
Fragments arranged by size:
6001 894 459 264 78
PmeI GTTT'AAAC
Cuts at: 4693 4693
Size: 7696
PmlI CAC'GTG
Cuts at: 2454 3297 2454
Size: 843 6853
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6835 6867 6835
Size: 32 7664
PpuMI rG'GwC_Cy
Cuts at: 835 2124 835
Size: 1289 6407
PshAI GACnn'nnGTC
Cuts at: 1907 1907
Size: 7696
PspOMI G'GGCC_C
Cuts at: 2517 2735 2855 2894 3056 3067 3183 3827 4038
Size: 218 120 39 162 11 116 644 211
Cuts at: 4038 2517
Size: 6175
Fragments arranged by size:
6175 644 218 211 162 120 116 39
11
PspXI vC'TCGA_Gb
Cuts at: 4652 4652
Size: 7696
PstI C_TGCA'G
Cuts at: 449 1284 2102 2285 2321 2579 3740 4184 4741
Size: 835 818 183 36 258 1161 444 557
Cuts at: 4741 5426 449
Size: 685 2719
Fragments arranged by size:
2719 1161 835 818 685 557 444 258
183 36
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7696
PvuII CAG'CTG
Cuts at: 463 2535 3756 3849 4233 5119 5479 463
Size: 2072 1221 93 384 886 360 2680
Fragments arranged by size:
2680 2072 1221 886 384 360 93
RsrII CG'GwC_CG
Cuts at: 5889 5889
Size: 7696
SacI G_AGCT'C
Cuts at: 668 1469 1770 3788 3812 4268 4708 668
Size: 801 301 2018 24 456 440 3656
Fragments arranged by size:
3656 2018 801 456 440 301 24
SalI G'TCGA_C
Cuts at: 4731 4731
Size: 7696
SanDI GG'GwC_CC
Cuts at: 2124 2124
Size: 7696
SapI GCTCTTCn'nnn_
Cuts at: 4359 5717 5927 4359
Size: 1358 210 6128
Fragments arranged by size:
6128 1358 210
SbfI CC_TGCA'GG
Cuts at: 2579 4741 2579
Size: 2162 5534
ScaI AGT'ACT
Cuts at: 7213 7213
Size: 7696
SexAI A'CCwGG_T
Cuts at: 1230 1412 1230
Size: 182 7514
SfcI C'TryA_G
Cuts at: 33 445 1280 1517 1873 2098 2281 2317 2575
Size: 412 835 237 356 225 183 36 258
Cuts at: 2575 2746 3736 4180 4737 5422 6417 6608 33
Size: 171 990 444 557 685 995 191 1121
Fragments arranged by size:
1121 995 990 835 685 557 444 412
356 258 237 225 191 183 171 36
SfiI GGCCn_nnn'nGGCC
Cuts at: 1592 4390 1592
Size: 2798 4898
SfoI GGC'GCC
Cuts at: 5126 5374 5126
Size: 248 7448
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7696
SmaI CCC'GGG
Cuts at: 3514 4381 4716 3514
Size: 867 335 6494
Fragments arranged by size:
6494 867 335
SmlI C'TyrA_G
Cuts at: 487 1947 3435 3690 4033 4207 4652 4849 5764
Size: 1460 1488 255 343 174 445 197 915
Cuts at: 5764 6258 6520 487
Size: 494 262 1663
Fragments arranged by size:
1663 1488 1460 915 494 445 343 262
255 197 174
SnaBI TAC'GTA
Cuts at: 4686 4686
Size: 7696
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7696
SphI G_CATG'C
Cuts at: 4414 4747 5247 4414
Size: 333 500 6863
Fragments arranged by size:
6863 500 333
StuI AGG'CCT
Cuts at: 917 1559 1866 3242 4078 4480 917
Size: 642 307 1376 836 402 4133
Fragments arranged by size:
4133 1376 836 642 402 307
StyI C'CwwG_G
Cuts at: 125 305 1365 2187 2382 3704 3831 4289 4329
Size: 180 1060 822 195 1322 127 458 40
Cuts at: 4329 4828 5182 125
Size: 499 354 2639
Fragments arranged by size:
2639 1322 1060 822 499 458 354 195
180 127 40
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2766 3589 5277 5410 5869 6080 2766
Size: 823 1688 133 459 211 4382
Fragments arranged by size:
4382 1688 823 459 211 133
TatI w'GTAC_w
Cuts at: 721 3843 7211 721
Size: 3122 3368 1206
Fragments arranged by size:
3368 3122 1206
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2086 2118 4332 4364 7213 7245 2086
Size: 32 2214 32 2849 32 2537
Fragments arranged by size:
2849 2537 2214 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 4643 5491 4643
Size: 848 6848
XbaI T'CTAG_A
Cuts at: 4725 4725
Size: 7696
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1363 2223 2370 3261 3329 5189 1363
Size: 860 147 891 68 1860 3870
Fragments arranged by size:
3870 1860 891 860 147 68
XhoI C'TCGA_G
Cuts at: 4652 4652
Size: 7696
XmaI C'CCGG_G
Cuts at: 3512 4379 4714 3512
Size: 867 335 6494
Fragments arranged by size:
6494 867 335
XmnI GAAnn'nnTTC
Cuts at: 3521 3521
Size: 7696
ZraI GAC'GTC
Cuts at: 4494 4494
Size: 7696
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NheI NruI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PspOMI
PspXI PstI PvuI PvuII RsrII SacI SalI SanDI
SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI
SmaI SmlI SnaBI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AflII AloI AscI AvrII BaeI BbsI BfrBI BsiWI
BssHII BstZ17I ClaI CspCI FseI FspAI HpaI MfeI
NdeI NotI NsiI PacI PsiI PsrI SacII SgrAI
SrfI SspI SwaI