Restriction map for
pF1KSDF0136
(Circular) (Six-base) MAPSORT of: pF1KSDF0136.seq Check: 3237 from: 1 to: 5554
pF1KSDF0136 5554 bp
With 182 enzymes: SgfI *
June 20, 2008 10:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1195 2136 2173 1195
Size: 941 37 4576
Fragments arranged by size:
4576 941 37
AccI GT'mk_AC
Cuts at: 2590 2590
Size: 5554
Acc65I G'GTAC_C
Cuts at: 2273 2568 2273
Size: 295 5259
AclI AA'CG_TT
Cuts at: 5394 5394
Size: 5554
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 847 1369 1882 3377 3809 4558 4826 847
Size: 522 513 1495 432 749 268 1575
Fragments arranged by size:
1575 1495 749 522 513 432 268
AflII C'TTAA_G
Cuts at: 633 633
Size: 5554
AflIII A'CryG_T
Cuts at: 2904 4010 4987 2904
Size: 1106 977 3471
Fragments arranged by size:
3471 1106 977
AgeI A'CCGG_T
Cuts at: 1775 2270 3942 1775
Size: 495 1672 3387
Fragments arranged by size:
3387 1672 495
AleI CACnn'nnGTG
Cuts at: 1198 1198
Size: 5554
AlwNI CAG_nnn'CTG
Cuts at: 138 591 804 1519 1770 1929 2857 4426 138
Size: 453 213 715 251 159 928 1569 1266
Fragments arranged by size:
1569 1266 928 715 453 251 213 159
ApaI G_GGCC'C
Cuts at: 190 943 976 1584 190
Size: 753 33 608 4160
Fragments arranged by size:
4160 753 608 33
ApaLI G'TGCA_C
Cuts at: 214 255 1107 2266 4324 214
Size: 41 852 1159 2058 1444
Fragments arranged by size:
2058 1444 1159 852 41
ApoI r'AATT_y
Cuts at: 2556 4907 5163 2556
Size: 2351 256 2947
Fragments arranged by size:
2947 2351 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5554
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5554
AvaI C'yCGr_G
Cuts at: 334 475 678 2157 2572 334
Size: 141 203 1479 415 3316
Fragments arranged by size:
3316 1479 415 203 141
BamHI G'GATC_C
Cuts at: 2577 2577
Size: 5554
BanI G'GyrC_C
Cuts at: 340 409 2037 2113 2273 2505 2568 2982 3230
Size: 69 1628 76 160 232 63 414 248
Cuts at: 3230 3265 340
Size: 35 2629
Fragments arranged by size:
2629 1628 414 248 232 160 76 69
63 35
BanII G_rGCy'C
Cuts at: 190 584 943 976 1004 1584 2566 3596 190
Size: 394 359 33 28 580 982 1030 2148
Fragments arranged by size:
2148 1030 982 580 394 359 33 28
BbeI G_GCGC'C
Cuts at: 2041 2117 2986 3234 2041
Size: 76 869 248 4361
Fragments arranged by size:
4361 869 248 76
BbsI GAAGACnn'nnnn_
Cuts at: 1266 1479 1989 2017 2070 2115 2292 1266
Size: 213 510 28 53 45 177 4528
Fragments arranged by size:
4528 510 213 177 53 45 28
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2482 2516 2482
Size: 34 5520
BciVI GTATCCnnnnn_n'
Cuts at: 3450 4213 5554 3450
Size: 763 1341 3450
Fragments arranged by size:
3450 1341 763
BclI T'GATC_A
Cuts at: 3072 3072
Size: 5554
BglI GCCn_nnn'nGGC
Cuts at: 704 799 848 2046 704
Size: 95 49 1198 4212
Fragments arranged by size:
4212 1198 95 49
BglII A'GATC_T
Cuts at: 3067 5106 3067
Size: 2039 3515
BlpI GC'TnA_GC
Cuts at: 2664 2664
Size: 5554
Bme1580I G_kGCm'C
Cuts at: 190 218 259 343 943 976 1111 1584 2270
Size: 28 41 84 600 33 135 473 686
Cuts at: 2270 3177 3270 4328 190
Size: 907 93 1058 1416
Fragments arranged by size:
1416 1058 907 686 600 473 135 93
84 41 33 28
BmgBI CAC'GTC
Cuts at: 1485 1485
Size: 5554
BmrI ACTGGGnnnn_n'
Cuts at: 417 2764 2845 3180 5326 417
Size: 2347 81 335 2146 645
Fragments arranged by size:
2347 2146 645 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3919 3919
Size: 5554
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 295 544 565 568 837 1018 1306 1633 1657
Size: 249 21 3 269 181 288 327 24
Cuts at: 1657 1927 2248 295
Size: 270 321 3601
Fragments arranged by size:
3601 327 321 288 270 269 249 181
24 21 3
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 865 1657 2368 2728 3607 4101 4399 865
Size: 792 711 360 879 494 298 2020
Fragments arranged by size:
2020 879 792 711 494 360 298
BsaI GGTCTCn'nnnn_
Cuts at: 157 2253 5258 157
Size: 2096 3005 453
Fragments arranged by size:
3005 2096 453
BsaAI yAC'GTr
Cuts at: 1647 2544 2905 1647
Size: 897 361 4296
Fragments arranged by size:
4296 897 361
BsaBI GATnn'nnATC
Cuts at: 6 3066 5105 6
Size: 3060 2039 455
Fragments arranged by size:
3060 2039 455
BsaHI Gr'CG_yC
Cuts at: 1727 2038 2114 2983 3231 3935 5431 1727
Size: 311 76 869 248 704 1496 1850
Fragments arranged by size:
1850 1496 869 704 311 248 76
BsaWI w'CCGG_w
Cuts at: 1775 2177 2270 2741 3262 3942 4216 4363 4774
Size: 402 93 471 521 680 274 147 411
Cuts at: 4774 1775
Size: 2555
Fragments arranged by size:
2555 680 521 471 411 402 274 147
93
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 562 565 568 994 1558 1881 1951 2482 2487
Size: 3 3 426 564 323 70 531 5
Cuts at: 2487 2509 562
Size: 22 3607
Fragments arranged by size:
3607 564 531 426 323 70 22 5
3 3
BseYI C'CCAG_C
Cuts at: 131 190 382 502 1425 1461 1710 1745 1938
Size: 59 192 120 923 36 249 35 193
Cuts at: 1938 2977 3735 4314 4810 131
Size: 1039 758 579 496 875
Fragments arranged by size:
1039 923 875 758 579 496 249 193
192 120 59 36 35
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 463 474 972 1993 463
Size: 11 498 1021 4024
Fragments arranged by size:
4024 1021 498 11
BsiEI CG_ry'CG
Cuts at: 104 3094 3140 4350 104
Size: 2990 46 1210 1308
Fragments arranged by size:
2990 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 218 259 352 584 863 1004 1111 1949 2074
Size: 41 93 232 279 141 107 838 125
Cuts at: 2074 2164 2270 2566 3344 3534 4328 218
Size: 90 106 296 778 190 794 1444
Fragments arranged by size:
1444 838 794 778 296 279 232 190
141 125 107 106 93 90 41
Bsp1286I G_dGCh'C
Cuts at: 190 218 259 343 352 584 863 943 976
Size: 28 41 84 9 232 279 80 33
Cuts at: 976 1004 1111 1584 1949 2074 2164 2270 2566
Size: 28 107 473 365 125 90 106 296
Cuts at: 2566 3177 3270 3344 3534 3596 4328 190
Size: 611 93 74 190 62 732 1416
Fragments arranged by size:
1416 732 611 473 365 296 279 232
190 125 107 106 93 90 84 80
74 62 41 33 28 28 9
BspEI T'CCGG_A
Cuts at: 2177 4774 2177
Size: 2597 2957
BspHI T'CATG_A
Cuts at: 1499 4730 1499
Size: 3231 2323
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1195 2136 2173 2602 2816 3118 3499 1195
Size: 941 37 429 214 302 381 3250
Fragments arranged by size:
3250 941 429 381 302 214 37
BsrBI CCG'CTC
Cuts at: 50 294 491 3844 3898 5384 5549 50
Size: 244 197 3353 54 1486 165 55
Fragments arranged by size:
3353 1486 244 197 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1889 2406 3464 1889
Size: 517 1058 3979
Fragments arranged by size:
3979 1058 517
BsrFI r'CCGG_y
Cuts at: 464 734 1775 2045 2203 2270 3550 3731 3942
Size: 270 1041 270 158 67 1280 181 211
Cuts at: 3942 464
Size: 2076
Fragments arranged by size:
2076 1280 1041 270 270 211 181 158
67
BsrGI T'GTAC_A
Cuts at: 1202 1202
Size: 5554
BssSI C'ACGA_G
Cuts at: 3823 4183 3823
Size: 360 5194
BstAPI GCAn_nnn'nTGC
Cuts at: 1106 2512 2929 1106
Size: 1406 417 3731
Fragments arranged by size:
3731 1406 417
BstBI TT'CG_AA
Cuts at: 110 110
Size: 5554
BstXI CCAn_nnnn'nTGG
Cuts at: 543 1081 2022 543
Size: 538 941 4075
Fragments arranged by size:
4075 941 538
BstYI r'GATC_y
Cuts at: 1 2577 3044 3067 3402 3648 4662 4748 4760
Size: 2576 467 23 335 246 1014 86 12
Cuts at: 4760 5106 1
Size: 346 449
Fragments arranged by size:
2576 1014 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 944 944
Size: 5554
BtgI C'CryG_G
Cuts at: 125 536 811 1472 125
Size: 411 275 661 4207
Fragments arranged by size:
4207 661 411 275
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3502 3683 3846 3502
Size: 181 163 5210
Fragments arranged by size:
5210 181 163
BtsI GCAGTG_nn'
Cuts at: 602 2795 602
Size: 2193 3361
DraI TTT'AAA
Cuts at: 2551 2551
Size: 5554
DrdI GACnn_nn'nnGTC
Cuts at: 3258 4118 3258
Size: 860 4694
EaeI y'GGCC_r
Cuts at: 353 371 396 732 1047 1361 1475 1524 1604
Size: 18 25 336 315 314 114 49 80
Cuts at: 1604 1649 1679 2024 2324 3137 3311 3702 3729
Size: 45 30 345 300 813 174 391 27
Cuts at: 3729 5076 353
Size: 1347 831
Fragments arranged by size:
1347 831 813 391 345 336 315 314
300 174 114 80 49 45 30 27
25 18
EagI C'GGCC_G
Cuts at: 3137 3137
Size: 5554
EarI CTCTTCn'nnn_
Cuts at: 661 1858 1954 3575 3785 661
Size: 1197 96 1621 210 2430
Fragments arranged by size:
2430 1621 1197 210 96
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 483 499 780 1887 3760 4072 4218 5364 483
Size: 16 281 1107 1873 312 146 1146 673
Fragments arranged by size:
1873 1146 1107 673 312 281 146 16
EcoICRI GAG'CTC
Cuts at: 582 1002 2564 582
Size: 420 1562 3572
Fragments arranged by size:
3572 1562 420
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 295 544 565 568 837 847 1018 1306 1369
Size: 249 21 3 269 10 171 288 63
Cuts at: 1369 1633 1657 1882 1927 2248 3377 3809 4558
Size: 264 24 225 45 321 1129 432 749
Cuts at: 4558 4826 295
Size: 268 1023
Fragments arranged by size:
1129 1023 749 432 321 288 269 268
264 249 225 171 63 45 24 21
10 3
EcoNI CCTnn'n_nnAGG
Cuts at: 547 574 1324 2383 547
Size: 27 750 1059 3718
Fragments arranged by size:
3718 1059 750 27
EcoO109I rG'GnC_Cy
Cuts at: 186 187 674 801 940 973 1580 1581 2239
Size: 1 487 127 139 33 607 1 658
Cuts at: 2239 2691 186
Size: 452 3049
Fragments arranged by size:
3049 658 607 487 452 139 127 33
1 1
EcoRI G'AATT_C
Cuts at: 2556 2556
Size: 5554
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3571 3571
Size: 5554
FspI TGC'GCA
Cuts at: 3333 3333
Size: 5554
HaeII r_GCGC'y
Cuts at: 784 819 2041 2117 2986 3234 4258 5237 784
Size: 35 1222 76 869 248 1024 979 1101
Fragments arranged by size:
1222 1101 1024 979 869 248 76 35
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 398 430 1494 1526 3394 3424 3426 3456 398
Size: 32 1064 32 1868 30 2 30 2496
Fragments arranged by size:
2496 1868 1064 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2591 2591
Size: 5554
HindIII A'AGCT_T
Cuts at: 2434 2434
Size: 5554
KasI G'GCGC_C
Cuts at: 2037 2113 2982 3230 2037
Size: 76 869 248 4361
Fragments arranged by size:
4361 869 248 76
KpnI G_GTAC'C
Cuts at: 2277 2572 2277
Size: 295 5259
MluI A'CGCG_T
Cuts at: 4987 4987
Size: 5554
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4225 4409 4225
Size: 184 5370
MscI TGG'CCA
Cuts at: 373 1526 1651 1681 2026 3313 373
Size: 1153 125 30 345 1287 2614
Fragments arranged by size:
2614 1287 1153 345 125 30
MslI CAynn'nnrTG
Cuts at: 1036 1198 2347 3541 3668 1036
Size: 162 1149 1194 127 2922
Fragments arranged by size:
2922 1194 1149 162 127
MspA1I CmG'CkG
Cuts at: 606 710 1374 2119 2663 2977 3337 4352 4597
Size: 104 664 745 544 314 360 1015 245
Cuts at: 4597 606
Size: 1563
Fragments arranged by size:
1563 1015 745 664 544 360 314 245
104
NaeI GCC'GGC
Cuts at: 466 736 2047 2205 3733 466
Size: 270 1311 158 1528 2287
Fragments arranged by size:
2287 1528 1311 270 158
NarI GG'CG_CC
Cuts at: 2038 2114 2983 3231 2038
Size: 76 869 248 4361
Fragments arranged by size:
4361 869 248 76
NcoI C'CATG_G
Cuts at: 125 125
Size: 5554
NgoMIV G'CCGG_C
Cuts at: 464 734 2045 2203 3731 464
Size: 270 1311 158 1528 2287
Fragments arranged by size:
2287 1528 1311 270 158
NruI TCG'CGA
Cuts at: 3915 3915
Size: 5554
NspI r_CATG'y
Cuts at: 381 618 1143 2605 3105 4014 381
Size: 237 525 1462 500 909 1921
Fragments arranged by size:
1921 1462 909 525 500 237
PasI CC'CwG_GG
Cuts at: 169 1115 2243 2261 169
Size: 946 1128 18 3462
Fragments arranged by size:
3462 1128 946 18
PciI A'CATG_T
Cuts at: 4010 4010
Size: 5554
PflMI CCAn_nnn'nTGG
Cuts at: 138 591 1710 138
Size: 453 1119 3982
Fragments arranged by size:
3982 1119 453
PfoI T'CCnGG_A
Cuts at: 239 1904 239
Size: 1665 3889
PmeI GTTT'AAAC
Cuts at: 2551 2551
Size: 5554
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4693 4725 4693
Size: 32 5522
PpuMI rG'GwC_Cy
Cuts at: 674 674
Size: 5554
PspOMI G'GGCC_C
Cuts at: 186 939 972 1580 186
Size: 753 33 608 4160
Fragments arranged by size:
4160 753 608 33
PspXI vC'TCGA_Gb
Cuts at: 2157 2157
Size: 5554
PstI C_TGCA'G
Cuts at: 284 800 1660 1736 2599 3284 284
Size: 516 860 76 863 685 2554
Fragments arranged by size:
2554 863 860 685 516 76
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5554
PvuII CAG'CTG
Cuts at: 1374 2977 3337 1374
Size: 1603 360 3591
Fragments arranged by size:
3591 1603 360
RsrII CG'GwC_CG
Cuts at: 3747 3747
Size: 5554
SacI G_AGCT'C
Cuts at: 584 1004 2566 584
Size: 420 1562 3572
Fragments arranged by size:
3572 1562 420
SalI G'TCGA_C
Cuts at: 2589 2589
Size: 5554
SapI GCTCTTCn'nnn_
Cuts at: 661 1954 3575 3785 661
Size: 1293 1621 210 2430
Fragments arranged by size:
2430 1621 1293 210
SbfI CC_TGCA'GG
Cuts at: 800 1736 2599 800
Size: 936 863 3755
Fragments arranged by size:
3755 936 863
ScaI AGT'ACT
Cuts at: 427 5071 427
Size: 4644 910
SfcI C'TryA_G
Cuts at: 33 280 796 1053 1656 1732 2595 3280 4275
Size: 247 516 257 603 76 863 685 995
Cuts at: 4275 4466 33
Size: 191 1121
Fragments arranged by size:
1121 995 863 685 603 516 257 247
191 76
SfoI GGC'GCC
Cuts at: 2039 2115 2984 3232 2039
Size: 76 869 248 4361
Fragments arranged by size:
4361 869 248 76
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5554
SmaI CCC'GGG
Cuts at: 2574 2574
Size: 5554
SmlI C'TyrA_G
Cuts at: 633 880 1672 2157 2383 2707 3622 4116 4378
Size: 247 792 485 226 324 915 494 262
Cuts at: 4378 633
Size: 1809
Fragments arranged by size:
1809 915 792 494 485 324 262 247
226
SnaBI TAC'GTA
Cuts at: 2544 2544
Size: 5554
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5554
SphI G_CATG'C
Cuts at: 381 2605 3105 381
Size: 2224 500 2830
Fragments arranged by size:
2830 2224 500
SspI AAT'ATT
Cuts at: 1335 1335
Size: 5554
StuI AGG'CCT
Cuts at: 995 995
Size: 5554
StyI C'CwwG_G
Cuts at: 125 2686 3040 125
Size: 2561 354 2639
Fragments arranged by size:
2639 2561 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 358 1042 1111 1128 2205 2253 3135 3268 3727
Size: 684 69 17 1077 48 882 133 459
Cuts at: 3727 3938 358
Size: 211 1974
Fragments arranged by size:
1974 1077 882 684 459 211 133 69
48 17
TatI w'GTAC_w
Cuts at: 425 1040 1202 5069 425
Size: 615 162 3867 910
Fragments arranged by size:
3867 910 615 162
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 350 382 1819 1851 5071 5103 350
Size: 32 1437 32 3220 32 801
Fragments arranged by size:
3220 1437 801 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1795 3349 1795
Size: 1554 4000
XbaI T'CTAG_A
Cuts at: 2583 2583
Size: 5554
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3047 3047
Size: 5554
XhoI C'TCGA_G
Cuts at: 2157 2157
Size: 5554
XmaI C'CCGG_G
Cuts at: 2572 2572
Size: 5554
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AleI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI
Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NruI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PspOMI PspXI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AfeI AhdI AlfI AloI AscI AvrII BaeI
BbvCI BfrBI BmtI Bpu10I BsaXI BsiWI BsmI BsmBI
BssHII BstEII BstZ17I ClaI CspCI DraIII EcoRV FseI
FspAI HpaI MfeI NdeI NheI NotI NsiI PacI
PmlI PshAI PsiI PsrI SacII SanDI SexAI SfiI
SgrAI SrfI SwaI XmnI ZraI