Restriction map for
pF1KSDF0166
(Circular) (Six-base) MAPSORT of: pF1KSDF0166.seq Check: 9997 from: 1 to: 4456
pF1KSDF0166 4456 bp
With 182 enzymes: SgfI *
June 20, 2008 10:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 387 1404 387
Size: 1017 3439
AccI GT'mk_AC
Cuts at: 1492 1492
Size: 4456
Acc65I G'GTAC_C
Cuts at: 1470 1470
Size: 4456
AclI AA'CG_TT
Cuts at: 4296 4296
Size: 4456
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 824 1016 2279 2711 3460 3728 824
Size: 192 1263 432 749 268 1552
Fragments arranged by size:
1552 1263 749 432 268 192
AflII C'TTAA_G
Cuts at: 624 624
Size: 4456
AflIII A'CryG_T
Cuts at: 1806 2912 3889 1806
Size: 1106 977 2373
Fragments arranged by size:
2373 1106 977
AgeI A'CCGG_T
Cuts at: 2844 2844
Size: 4456
AlwNI CAG_nnn'CTG
Cuts at: 1759 3328 1759
Size: 1569 2887
ApaI G_GGCC'C
Cuts at: 259 259
Size: 4456
ApaLI G'TGCA_C
Cuts at: 3226 3226
Size: 4456
ApoI r'AATT_y
Cuts at: 322 538 916 987 1439 1458 3809 4065 322
Size: 216 378 71 452 19 2351 256 713
Fragments arranged by size:
2351 713 452 378 256 216 71 19
AseI AT'TA_AT
Cuts at: 20 395 20
Size: 375 4081
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4456
AvaI C'yCGr_G
Cuts at: 251 1474 251
Size: 1223 3233
BamHI G'GATC_C
Cuts at: 657 1479 657
Size: 822 3634
BanI G'GyrC_C
Cuts at: 1470 1884 2132 2167 1470
Size: 414 248 35 3759
Fragments arranged by size:
3759 414 248 35
BanII G_rGCy'C
Cuts at: 259 1468 2498 259
Size: 1209 1030 2217
Fragments arranged by size:
2217 1209 1030
BbeI G_GCGC'C
Cuts at: 1888 2136 1888
Size: 248 4208
BbsI GAAGACnn'nnnn_
Cuts at: 407 407
Size: 4456
BciVI GTATCCnnnnn_n'
Cuts at: 2352 3115 4456 2352
Size: 763 1341 2352
Fragments arranged by size:
2352 1341 763
BclI T'GATC_A
Cuts at: 1974 1974
Size: 4456
BglII A'GATC_T
Cuts at: 1969 4008 1969
Size: 2039 2417
BlpI GC'TnA_GC
Cuts at: 1566 1566
Size: 4456
Bme1580I G_kGCm'C
Cuts at: 259 2079 2172 3230 259
Size: 1820 93 1058 1485
Fragments arranged by size:
1820 1485 1058 93
BmrI ACTGGGnnnn_n'
Cuts at: 1666 1747 2082 4228 1666
Size: 81 335 2146 1894
Fragments arranged by size:
2146 1894 335 81
BmtI G_CTAG'C
Cuts at: 736 736
Size: 4456
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2821 2821
Size: 4456
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1630 2509 3003 3301 1630
Size: 879 494 298 2785
Fragments arranged by size:
2785 879 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 4160 4160
Size: 4456
BsaAI yAC'GTr
Cuts at: 1446 1807 1446
Size: 361 4095
BsaBI GATnn'nnATC
Cuts at: 6 1968 4007 6
Size: 1962 2039 455
Fragments arranged by size:
2039 1962 455
BsaHI Gr'CG_yC
Cuts at: 1089 1885 2133 2837 4333 1089
Size: 796 248 704 1496 1212
Fragments arranged by size:
1496 1212 796 704 248
BsaWI w'CCGG_w
Cuts at: 1643 2164 2844 3118 3265 3676 1643
Size: 521 680 274 147 411 2423
Fragments arranged by size:
2423 680 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1304 1304
Size: 4456
BseYI C'CCAG_C
Cuts at: 266 1879 2637 3216 3712 266
Size: 1613 758 579 496 1010
Fragments arranged by size:
1613 1010 758 579 496
BsiEI CG_ry'CG
Cuts at: 104 239 1996 2042 3252 104
Size: 135 1757 46 1210 1308
Fragments arranged by size:
1757 1308 1210 135 46
BsiHKAI G_wGCw'C
Cuts at: 1468 2246 2436 3230 1468
Size: 778 190 794 2694
Fragments arranged by size:
2694 794 778 190
Bsp1286I G_dGCh'C
Cuts at: 259 1468 2079 2172 2246 2436 2498 3230 259
Size: 1209 611 93 74 190 62 732 1485
Fragments arranged by size:
1485 1209 732 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3676 3676
Size: 4456
BspHI T'CATG_A
Cuts at: 581 3632 581
Size: 3051 1405
BspMI ACCTGCnnnn'nnnn_
Cuts at: 387 1404 1504 1718 2020 2401 387
Size: 1017 100 214 302 381 2442
Fragments arranged by size:
2442 1017 381 302 214 100
BsrBI CCG'CTC
Cuts at: 50 2746 2800 4286 4451 50
Size: 2696 54 1486 165 55
Fragments arranged by size:
2696 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2366 2366
Size: 4456
BsrFI r'CCGG_y
Cuts at: 2452 2633 2844 2452
Size: 181 211 4064
Fragments arranged by size:
4064 211 181
BssSI C'ACGA_G
Cuts at: 1163 2725 3085 1163
Size: 1562 360 2534
Fragments arranged by size:
2534 1562 360
BstAPI GCAn_nnn'nTGC
Cuts at: 276 1177 1384 1831 276
Size: 901 207 447 2901
Fragments arranged by size:
2901 901 447 207
BstBI TT'CG_AA
Cuts at: 110 110
Size: 4456
BstYI r'GATC_y
Cuts at: 1 342 657 1479 1946 1969 2304 2550 3564
Size: 341 315 822 467 23 335 246 1014
Cuts at: 3564 3650 3662 4008 1
Size: 86 12 346 449
Fragments arranged by size:
1014 822 467 449 346 341 335 315
246 86 23 12
BtgI C'CryG_G
Cuts at: 125 162 651 125
Size: 37 489 3930
Fragments arranged by size:
3930 489 37
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2404 2585 2748 2404
Size: 181 163 4112
Fragments arranged by size:
4112 181 163
BtsI GCAGTG_nn'
Cuts at: 1697 1697
Size: 4456
DraI TTT'AAA
Cuts at: 1049 1080 1322 1371 1453 1049
Size: 31 242 49 82 4052
Fragments arranged by size:
4052 242 82 49 31
DrdI GACnn_nn'nnGTC
Cuts at: 2160 3020 2160
Size: 860 3596
EaeI y'GGCC_r
Cuts at: 236 2039 2213 2604 2631 3978 236
Size: 1803 174 391 27 1347 714
Fragments arranged by size:
1803 1347 714 391 174 27
EagI C'GGCC_G
Cuts at: 236 2039 236
Size: 1803 2653
EarI CTCTTCn'nnn_
Cuts at: 289 759 764 800 2477 2687 289
Size: 470 5 36 1677 210 2058
Fragments arranged by size:
2058 1677 470 210 36 5
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2662 2974 3120 4266 2662
Size: 312 146 1146 2852
Fragments arranged by size:
2852 1146 312 146
EcoICRI GAG'CTC
Cuts at: 1466 1466
Size: 4456
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 824 1016 2279 2711 3460 3728 824
Size: 192 1263 432 749 268 1552
Fragments arranged by size:
1552 1263 749 432 268 192
EcoO109I rG'GnC_Cy
Cuts at: 135 255 1593 135
Size: 120 1338 2998
Fragments arranged by size:
2998 1338 120
EcoRI G'AATT_C
Cuts at: 538 916 1458 538
Size: 378 542 3536
Fragments arranged by size:
3536 542 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2473 2473
Size: 4456
FspI TGC'GCA
Cuts at: 2235 2235
Size: 4456
HaeII r_GCGC'y
Cuts at: 1888 2136 3160 4139 1888
Size: 248 1024 979 2205
Fragments arranged by size:
2205 1024 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 649 681 686 718 2296 2326 2328 2358 649
Size: 32 5 32 1578 30 2 30 2747
Fragments arranged by size:
2747 1578 32 32 30 30 5 2
HincII GTy'rAC
Cuts at: 303 1071 1493 303
Size: 768 422 3266
Fragments arranged by size:
3266 768 422
HindIII A'AGCT_T
Cuts at: 1121 1151 1346 1121
Size: 30 195 4231
Fragments arranged by size:
4231 195 30
KasI G'GCGC_C
Cuts at: 1884 2132 1884
Size: 248 4208
KpnI G_GTAC'C
Cuts at: 1474 1474
Size: 4456
MluI A'CGCG_T
Cuts at: 3889 3889
Size: 4456
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1065 1264 3127 3311 1065
Size: 199 1863 184 2210
Fragments arranged by size:
2210 1863 199 184
MscI TGG'CCA
Cuts at: 2215 2215
Size: 4456
MslI CAynn'nnrTG
Cuts at: 1097 1250 2443 2570 1097
Size: 153 1193 127 2983
Fragments arranged by size:
2983 1193 153 127
MspA1I CmG'CkG
Cuts at: 1565 1879 2239 3254 3499 1565
Size: 314 360 1015 245 2522
Fragments arranged by size:
2522 1015 360 314 245
NaeI GCC'GGC
Cuts at: 2635 2635
Size: 4456
NarI GG'CG_CC
Cuts at: 1885 2133 1885
Size: 248 4208
NcoI C'CATG_G
Cuts at: 125 651 125
Size: 526 3930
NgoMIV G'CCGG_C
Cuts at: 2633 2633
Size: 4456
NheI G'CTAG_C
Cuts at: 732 732
Size: 4456
NruI TCG'CGA
Cuts at: 2817 2817
Size: 4456
NspI r_CATG'y
Cuts at: 1507 2007 2916 1507
Size: 500 909 3047
Fragments arranged by size:
3047 909 500
PciI A'CATG_T
Cuts at: 2912 2912
Size: 4456
PfoI T'CCnGG_A
Cuts at: 361 903 361
Size: 542 3914
PmeI GTTT'AAAC
Cuts at: 1453 1453
Size: 4456
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3595 3627 3595
Size: 32 4424
PpuMI rG'GwC_Cy
Cuts at: 135 135
Size: 4456
PspOMI G'GGCC_C
Cuts at: 255 255
Size: 4456
PstI C_TGCA'G
Cuts at: 1501 2186 1501
Size: 685 3771
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4456
PvuII CAG'CTG
Cuts at: 1879 2239 1879
Size: 360 4096
RsrII CG'GwC_CG
Cuts at: 2649 2649
Size: 4456
SacI G_AGCT'C
Cuts at: 1468 1468
Size: 4456
SalI G'TCGA_C
Cuts at: 1491 1491
Size: 4456
SapI GCTCTTCn'nnn_
Cuts at: 759 800 2477 2687 759
Size: 41 1677 210 2528
Fragments arranged by size:
2528 1677 210 41
SbfI CC_TGCA'GG
Cuts at: 1501 1501
Size: 4456
ScaI AGT'ACT
Cuts at: 3973 3973
Size: 4456
SfcI C'TryA_G
Cuts at: 33 822 1497 2182 3177 3368 33
Size: 789 675 685 995 191 1121
Fragments arranged by size:
1121 995 789 685 675 191
SfoI GGC'GCC
Cuts at: 1886 2134 1886
Size: 248 4208
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4456
SmaI CCC'GGG
Cuts at: 253 1476 253
Size: 1223 3233
SmlI C'TyrA_G
Cuts at: 624 1609 2524 3018 3280 624
Size: 985 915 494 262 1800
Fragments arranged by size:
1800 985 915 494 262
SnaBI TAC'GTA
Cuts at: 1446 1446
Size: 4456
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4456
SphI G_CATG'C
Cuts at: 1507 2007 1507
Size: 500 3956
StuI AGG'CCT
Cuts at: 974 974
Size: 4456
StyI C'CwwG_G
Cuts at: 125 651 887 1588 1942 125
Size: 526 236 701 354 2639
Fragments arranged by size:
2639 701 526 354 236
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2037 2170 2629 2840 2037
Size: 133 459 211 3653
Fragments arranged by size:
3653 459 211 133
TatI w'GTAC_w
Cuts at: 3971 3971
Size: 4456
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1079 1111 3973 4005 1079
Size: 32 2862 32 1530
Fragments arranged by size:
2862 1530 32 32
Tth111I GACn'n_nGTC
Cuts at: 2251 2251
Size: 4456
XbaI T'CTAG_A
Cuts at: 924 1485 924
Size: 561 3895
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1949 1949
Size: 4456
XmaI C'CCGG_G
Cuts at: 251 1474 251
Size: 1223 3233
XmnI GAAnn'nnTTC
Cuts at: 525 809 920 525
Size: 284 111 4061
Fragments arranged by size:
4061 284 111
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BglII BlpI
Bme1580I BmrI BmtI BplI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NheI
NruI NspI PciI PfoI PmeI PpiI PpuMI PspOMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SnaBI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AhdI AleI AlfI AloI AscI AvrII
BaeI BbvCI BcgI BfrBI BglI BmgBI BpmI Bpu10I
BsaXI BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HpaI MfeI NdeI NotI NsiI PacI
PasI PflMI PmlI PshAI PsiI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SrfI SspI SwaI XhoI
ZraI