Restriction map for
pF1KSDF0168
(Circular) (Six-base) MAPSORT of: pF1KSDF0168.seq Check: 3762 from: 1 to: 4840
pF1KSDF0168 4840 bp
With 182 enzymes: SgfI *
June 20, 2008 10:36 ..
AccI GT'mk_AC
Cuts at: 1876 1876
Size: 4840
Acc65I G'GTAC_C
Cuts at: 1854 1854
Size: 4840
AclI AA'CG_TT
Cuts at: 4680 4680
Size: 4840
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 316 1075 1084 2663 3095 3844 4112 316
Size: 759 9 1579 432 749 268 1044
Fragments arranged by size:
1579 1044 759 749 432 268 9
AflIII A'CryG_T
Cuts at: 557 1319 2190 3296 4273 557
Size: 762 871 1106 977 1124
Fragments arranged by size:
1124 1106 977 871 762
AgeI A'CCGG_T
Cuts at: 3228 3228
Size: 4840
AhdI GACnn_n'nnGTC
Cuts at: 376 822 376
Size: 446 4394
AleI CACnn'nnGTG
Cuts at: 1186 1186
Size: 4840
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 735 735
Size: 4840
AlwNI CAG_nnn'CTG
Cuts at: 516 810 2143 3712 516
Size: 294 1333 1569 1644
Fragments arranged by size:
1644 1569 1333 294
ApaI G_GGCC'C
Cuts at: 873 873
Size: 4840
ApaLI G'TGCA_C
Cuts at: 571 1015 3610 571
Size: 444 2595 1801
Fragments arranged by size:
2595 1801 444
ApoI r'AATT_y
Cuts at: 1842 4193 4449 1842
Size: 2351 256 2233
Fragments arranged by size:
2351 2233 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4840
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4840
AvaI C'yCGr_G
Cuts at: 613 772 1675 1858 613
Size: 159 903 183 3595
Fragments arranged by size:
3595 903 183 159
BamHI G'GATC_C
Cuts at: 240 491 1863 240
Size: 251 1372 3217
Fragments arranged by size:
3217 1372 251
BanI G'GyrC_C
Cuts at: 609 1435 1854 2268 2516 2551 609
Size: 826 419 414 248 35 2898
Fragments arranged by size:
2898 826 419 414 248 35
BanII G_rGCy'C
Cuts at: 284 524 873 952 1852 2882 284
Size: 240 349 79 900 1030 2242
Fragments arranged by size:
2242 1030 900 349 240 79
BbeI G_GCGC'C
Cuts at: 2272 2520 2272
Size: 248 4592
BbsI GAAGACnn'nnnn_
Cuts at: 671 671
Size: 4840
BbvCI CC'TCA_GC
Cuts at: 172 883 172
Size: 711 4129
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1425 1459 1425
Size: 34 4806
BciVI GTATCCnnnnn_n'
Cuts at: 2736 3499 4840 2736
Size: 763 1341 2736
Fragments arranged by size:
2736 1341 763
BclI T'GATC_A
Cuts at: 2358 2358
Size: 4840
BfrBI ATG'CAT
Cuts at: 233 233
Size: 4840
BglI GCCn_nnn'nGGC
Cuts at: 136 868 1215 1481 136
Size: 732 347 266 3495
Fragments arranged by size:
3495 732 347 266
BglII A'GATC_T
Cuts at: 2353 4392 2353
Size: 2039 2801
BlpI GC'TnA_GC
Cuts at: 358 946 1950 358
Size: 588 1004 3248
Fragments arranged by size:
3248 1004 588
Bme1580I G_kGCm'C
Cuts at: 575 614 873 1019 1685 2463 2556 3614 575
Size: 39 259 146 666 778 93 1058 1801
Fragments arranged by size:
1801 1058 778 666 259 146 93 39
BmgBI CAC'GTC
Cuts at: 819 1074 1392 819
Size: 255 318 4267
Fragments arranged by size:
4267 318 255
BmrI ACTGGGnnnn_n'
Cuts at: 537 724 2050 2131 2466 4612 537
Size: 187 1326 81 335 2146 765
Fragments arranged by size:
2146 1326 765 335 187 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3205 3205
Size: 4840
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 409 880 1757 409
Size: 471 877 3492
Fragments arranged by size:
3492 877 471
Bpu10I CC'TnA_GC
Cuts at: 172 787 883 1195 172
Size: 615 96 312 3817
Fragments arranged by size:
3817 615 312 96
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 247 691 1374 2014 2893 3387 3685 247
Size: 444 683 640 879 494 298 1402
Fragments arranged by size:
1402 879 683 640 494 444 298
BsaI GGTCTCn'nnnn_
Cuts at: 1024 4544 1024
Size: 3520 1320
BsaAI yAC'GTr
Cuts at: 560 1020 1173 1322 1830 2191 560
Size: 460 153 149 508 361 3209
Fragments arranged by size:
3209 508 460 361 153 149
BsaBI GATnn'nnATC
Cuts at: 6 2352 4391 6
Size: 2346 2039 455
Fragments arranged by size:
2346 2039 455
BsaHI Gr'CG_yC
Cuts at: 1442 2269 2517 3221 4717 1442
Size: 827 248 704 1496 1565
Fragments arranged by size:
1565 1496 827 704 248
BsaWI w'CCGG_w
Cuts at: 2027 2548 3228 3502 3649 4060 2027
Size: 521 680 274 147 411 2807
Fragments arranged by size:
2807 680 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 139 160 1237 139
Size: 21 1077 3742
Fragments arranged by size:
3742 1077 21
BseYI C'CCAG_C
Cuts at: 459 478 621 920 1589 2263 3021 3600 4096
Size: 19 143 299 669 674 758 579 496
Cuts at: 4096 459
Size: 1203
Fragments arranged by size:
1203 758 674 669 579 496 299 143
19
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 127 823 1042 1150 127
Size: 696 219 108 3817
Fragments arranged by size:
3817 696 219 108
BsiEI CG_ry'CG
Cuts at: 104 796 800 1257 2380 2426 3636 104
Size: 692 4 457 1123 46 1210 1308
Fragments arranged by size:
1308 1210 1123 692 457 46 4
BsiHKAI G_wGCw'C
Cuts at: 359 575 952 1019 1229 1571 1852 2630 2820
Size: 216 377 67 210 342 281 778 190
Cuts at: 2820 3614 359
Size: 794 1585
Fragments arranged by size:
1585 794 778 377 342 281 216 210
190 67
BsmBI CGTCTCn'nnnn_
Cuts at: 809 1591 809
Size: 782 4058
Bsp1286I G_dGCh'C
Cuts at: 284 359 524 575 614 873 952 1019 1229
Size: 75 165 51 39 259 79 67 210
Cuts at: 1229 1571 1685 1852 2463 2556 2630 2820 2882
Size: 342 114 167 611 93 74 190 62
Cuts at: 2882 3614 284
Size: 732 1510
Fragments arranged by size:
1510 732 611 342 259 210 190 167
165 114 93 79 75 74 67 62
51 39
BspEI T'CCGG_A
Cuts at: 4060 4060
Size: 4840
BspHI T'CATG_A
Cuts at: 4016 4016
Size: 4840
BspMI ACCTGCnnnn'nnnn_
Cuts at: 935 1888 2102 2404 2785 935
Size: 953 214 302 381 2990
Fragments arranged by size:
2990 953 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1094 3130 3184 4670 4835 50
Size: 1044 2036 54 1486 165 55
Fragments arranged by size:
2036 1486 1044 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1399 2750 1399
Size: 1351 3489
BsrFI r'CCGG_y
Cuts at: 791 795 1098 1176 1348 1438 1500 1561 2836
Size: 4 303 78 172 90 62 61 1275
Cuts at: 2836 3017 3228 791
Size: 181 211 2403
Fragments arranged by size:
2403 1275 303 211 181 172 90 78
62 61 4
BssHII G'CGCG_C
Cuts at: 936 938 1298 1620 936
Size: 2 360 322 4156
Fragments arranged by size:
4156 360 322 2
BssSI C'ACGA_G
Cuts at: 1812 3109 3469 1812
Size: 1297 360 3183
Fragments arranged by size:
3183 1297 360
BstAPI GCAn_nnn'nTGC
Cuts at: 1014 1173 2215 1014
Size: 159 1042 3639
Fragments arranged by size:
3639 1042 159
BstBI TT'CG_AA
Cuts at: 110 110
Size: 4840
BstEII G'GTnAC_C
Cuts at: 270 378 270
Size: 108 4732
BstYI r'GATC_y
Cuts at: 1 240 491 1863 2330 2353 2688 2934 3948
Size: 239 251 1372 467 23 335 246 1014
Cuts at: 3948 4034 4046 4392 1
Size: 86 12 346 449
Fragments arranged by size:
1372 1014 467 449 346 335 251 246
239 86 23 12
BtgI C'CryG_G
Cuts at: 125 983 125
Size: 858 3982
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 377 745 1006 2788 2969 3132 377
Size: 368 261 1782 181 163 2085
Fragments arranged by size:
2085 1782 368 261 181 163
BtsI GCAGTG_nn'
Cuts at: 2081 2081
Size: 4840
DraI TTT'AAA
Cuts at: 1837 1837
Size: 4840
DraIII CAC_nnn'GTG
Cuts at: 579 1014 1173 579
Size: 435 159 4246
Fragments arranged by size:
4246 435 159
DrdI GACnn_nn'nnGTC
Cuts at: 1534 2544 3404 1534
Size: 1010 860 2970
Fragments arranged by size:
2970 1010 860
EaeI y'GGCC_r
Cuts at: 332 344 793 797 1178 1239 1545 1667 1724
Size: 12 449 4 381 61 306 122 57
Cuts at: 1724 2423 2597 2988 3015 4362 332
Size: 699 174 391 27 1347 810
Fragments arranged by size:
1347 810 699 449 391 381 306 174
122 61 57 27 12 4
EagI C'GGCC_G
Cuts at: 793 797 2423 793
Size: 4 1626 3210
Fragments arranged by size:
3210 1626 4
EarI CTCTTCn'nnn_
Cuts at: 1358 2861 3071 1358
Size: 1503 210 3127
Fragments arranged by size:
3127 1503 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1474 3046 3358 3504 4650 1474
Size: 1572 312 146 1146 1664
Fragments arranged by size:
1664 1572 1146 312 146
EcoICRI GAG'CTC
Cuts at: 950 1850 950
Size: 900 3940
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 316 409 880 1075 1084 1757 2663 3095 3844
Size: 93 471 195 9 673 906 432 749
Cuts at: 3844 4112 316
Size: 268 1044
Fragments arranged by size:
1044 906 749 673 471 432 268 195
93 9
EcoNI CCTnn'n_nnAGG
Cuts at: 1750 1750
Size: 4840
EcoO109I rG'GnC_Cy
Cuts at: 513 693 861 924 1208 1651 1817 1977 513
Size: 180 168 63 284 443 166 160 3376
Fragments arranged by size:
3376 443 284 180 168 166 160 63
EcoRI G'AATT_C
Cuts at: 1842 1842
Size: 4840
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2857 2857
Size: 4840
FseI GG_CCGG'CC
Cuts at: 799 799
Size: 4840
FspI TGC'GCA
Cuts at: 472 589 2619 472
Size: 117 2030 2693
Fragments arranged by size:
2693 2030 117
FspAI rTGC'GCAy
Cuts at: 472 472
Size: 4840
HaeII r_GCGC'y
Cuts at: 326 1156 1384 1507 1618 2272 2520 3544 4523
Size: 830 228 123 111 654 248 1024 979
Cuts at: 4523 326
Size: 643
Fragments arranged by size:
1024 979 830 654 643 248 228 123
111
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 362 394 808 840 1246 1278 2680 2710 2712
Size: 32 414 32 406 32 1402 30 2
Cuts at: 2712 2742 362
Size: 30 2460
Fragments arranged by size:
2460 1402 414 406 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1877 1877
Size: 4840
HindIII A'AGCT_T
Cuts at: 1197 1197
Size: 4840
KasI G'GCGC_C
Cuts at: 2268 2516 2268
Size: 248 4592
KpnI G_GTAC'C
Cuts at: 1858 1858
Size: 4840
MluI A'CGCG_T
Cuts at: 4273 4273
Size: 4840
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1307 3511 3695 1307
Size: 2204 184 2452
Fragments arranged by size:
2452 2204 184
MscI TGG'CCA
Cuts at: 1726 2599 1726
Size: 873 3967
MslI CAynn'nnrTG
Cuts at: 472 1186 1715 2827 2954 472
Size: 714 529 1112 127 2358
Fragments arranged by size:
2358 1112 714 529 127
MspA1I CmG'CkG
Cuts at: 258 878 1131 1949 2263 2623 3638 3883 258
Size: 620 253 818 314 360 1015 245 1215
Fragments arranged by size:
1215 1015 818 620 360 314 253 245
NaeI GCC'GGC
Cuts at: 793 797 1178 1350 1502 1563 3019 793
Size: 4 381 172 152 61 1456 2614
Fragments arranged by size:
2614 1456 381 172 152 61 4
NarI GG'CG_CC
Cuts at: 2269 2517 2269
Size: 248 4592
NcoI C'CATG_G
Cuts at: 125 125
Size: 4840
NgoMIV G'CCGG_C
Cuts at: 791 795 1176 1348 1500 1561 3017 791
Size: 4 381 172 152 61 1456 2614
Fragments arranged by size:
2614 1456 381 172 152 61 4
NruI TCG'CGA
Cuts at: 3201 3201
Size: 4840
NsiI A_TGCA'T
Cuts at: 235 235
Size: 4840
NspI r_CATG'y
Cuts at: 237 477 1146 1714 1891 2391 3300 237
Size: 240 669 568 177 500 909 1777
Fragments arranged by size:
1777 909 669 568 500 240 177
PasI CC'CwG_GG
Cuts at: 999 999
Size: 4840
PciI A'CATG_T
Cuts at: 3296 3296
Size: 4840
PflMI CCAn_nnn'nTGG
Cuts at: 516 516
Size: 4840
PfoI T'CCnGG_A
Cuts at: 386 386
Size: 4840
PmeI GTTT'AAAC
Cuts at: 1837 1837
Size: 4840
PmlI CAC'GTG
Cuts at: 560 1020 1322 560
Size: 460 302 4078
Fragments arranged by size:
4078 460 302
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3979 4011 3979
Size: 32 4808
PpuMI rG'GwC_Cy
Cuts at: 924 1817 924
Size: 893 3947
PshAI GACnn'nnGTC
Cuts at: 1603 1603
Size: 4840
PspOMI G'GGCC_C
Cuts at: 869 869
Size: 4840
PstI C_TGCA'G
Cuts at: 724 1885 2570 724
Size: 1161 685 2994
Fragments arranged by size:
2994 1161 685
PvuI CG_AT'CG
Cuts at: 104 1257 104
Size: 1153 3687
PvuII CAG'CTG
Cuts at: 258 878 2263 2623 258
Size: 620 1385 360 2475
Fragments arranged by size:
2475 1385 620 360
RsrII CG'GwC_CG
Cuts at: 1096 3033 1096
Size: 1937 2903
SacI G_AGCT'C
Cuts at: 952 1852 952
Size: 900 3940
SalI G'TCGA_C
Cuts at: 1875 1875
Size: 4840
SapI GCTCTTCn'nnn_
Cuts at: 2861 3071 2861
Size: 210 4630
SbfI CC_TGCA'GG
Cuts at: 1885 1885
Size: 4840
ScaI AGT'ACT
Cuts at: 4357 4357
Size: 4840
SfcI C'TryA_G
Cuts at: 33 720 1881 2566 3561 3752 33
Size: 687 1161 685 995 191 1121
Fragments arranged by size:
1161 1121 995 687 685 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 136 868 136
Size: 732 4108
SfoI GGC'GCC
Cuts at: 2270 2518 2270
Size: 248 4592
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4840
SgrAI Cr'CCGG_yG
Cuts at: 1438 1500 1438
Size: 62 4778
SmaI CCC'GGG
Cuts at: 1860 1860
Size: 4840
SmlI C'TyrA_G
Cuts at: 262 706 1353 1993 2908 3402 3664 262
Size: 444 647 640 915 494 262 1438
Fragments arranged by size:
1438 915 647 640 494 444 262
SnaBI TAC'GTA
Cuts at: 1830 1830
Size: 4840
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4840
SphI G_CATG'C
Cuts at: 237 477 1891 2391 237
Size: 240 1414 500 2686
Fragments arranged by size:
2686 1414 500 240
StyI C'CwwG_G
Cuts at: 125 350 1184 1779 1972 2326 125
Size: 225 834 595 193 354 2639
Fragments arranged by size:
2639 834 595 354 225 193
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 556 904 1581 2421 2554 3013 3224 556
Size: 348 677 840 133 459 211 2172
Fragments arranged by size:
2172 840 677 459 348 211 133
TatI w'GTAC_w
Cuts at: 899 4355 899
Size: 3456 1384
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1088 1120 4357 4389 1088
Size: 32 3237 32 1539
Fragments arranged by size:
3237 1539 32 32
Tth111I GACn'n_nGTC
Cuts at: 2635 2635
Size: 4840
XbaI T'CTAG_A
Cuts at: 1869 1869
Size: 4840
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 132 2333 132
Size: 2201 2639
XmaI C'CCGG_G
Cuts at: 1858 1858
Size: 4840
XmnI GAAnn'nnTTC
Cuts at: 767 767
Size: 4840
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AleI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FseI FspI FspAI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NruI NsiI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PspOMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AloI AscI AvrII BaeI
BmtI BsaXI BsiWI BsmI BsrGI BstXI BstZ17I Bsu36I
ClaI CspCI EcoRV HpaI MfeI NdeI NheI NotI
PacI PsiI PspXI PsrI SacII SanDI SexAI SrfI
SspI StuI SwaI XhoI ZraI