Restriction map for
pF1KSDF0183
(Circular) (Six-base) MAPSORT of: pF1KSDF0183.seq Check: 7691 from: 1 to: 3817
pF1KSDF0183 3817 bp
With 182 enzymes: SgfI *
June 20, 2008 10:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 402 423 402
Size: 21 3796
AatII G_ACGT'C
Cuts at: 557 557
Size: 3817
AccI GT'mk_AC
Cuts at: 853 853
Size: 3817
Acc65I G'GTAC_C
Cuts at: 831 831
Size: 3817
AclI AA'CG_TT
Cuts at: 3657 3657
Size: 3817
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 721 1640 2072 2821 3089 721
Size: 919 432 749 268 1449
Fragments arranged by size:
1449 919 749 432 268
AflIII A'CryG_T
Cuts at: 1167 2273 3250 1167
Size: 1106 977 1734
Fragments arranged by size:
1734 1106 977
AgeI A'CCGG_T
Cuts at: 2205 2205
Size: 3817
AlwNI CAG_nnn'CTG
Cuts at: 1120 2689 1120
Size: 1569 2248
ApaLI G'TGCA_C
Cuts at: 2587 2587
Size: 3817
ApoI r'AATT_y
Cuts at: 819 3170 3426 819
Size: 2351 256 1210
Fragments arranged by size:
2351 1210 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3817
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3817
AvaI C'yCGr_G
Cuts at: 192 835 192
Size: 643 3174
BamHI G'GATC_C
Cuts at: 840 840
Size: 3817
BanI G'GyrC_C
Cuts at: 187 831 1245 1493 1528 187
Size: 644 414 248 35 2476
Fragments arranged by size:
2476 644 414 248 35
BanII G_rGCy'C
Cuts at: 377 829 1859 377
Size: 452 1030 2335
Fragments arranged by size:
2335 1030 452
BbeI G_GCGC'C
Cuts at: 191 1249 1497 191
Size: 1058 248 2511
Fragments arranged by size:
2511 1058 248
BciVI GTATCCnnnnn_n'
Cuts at: 403 1713 2476 3817 403
Size: 1310 763 1341 403
Fragments arranged by size:
1341 1310 763 403
BclI T'GATC_A
Cuts at: 1335 1335
Size: 3817
BglII A'GATC_T
Cuts at: 1330 3369 1330
Size: 2039 1778
BlpI GC'TnA_GC
Cuts at: 927 927
Size: 3817
Bme1580I G_kGCm'C
Cuts at: 1440 1533 2591 1440
Size: 93 1058 2666
Fragments arranged by size:
2666 1058 93
BmrI ACTGGGnnnn_n'
Cuts at: 1027 1108 1443 3589 1027
Size: 81 335 2146 1255
Fragments arranged by size:
2146 1255 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2182 2182
Size: 3817
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 991 1870 2364 2662 991
Size: 879 494 298 2146
Fragments arranged by size:
2146 879 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 340 3521 340
Size: 3181 636
BsaAI yAC'GTr
Cuts at: 807 1168 807
Size: 361 3456
BsaBI GATnn'nnATC
Cuts at: 6 1329 3368 6
Size: 1323 2039 455
Fragments arranged by size:
2039 1323 455
BsaHI Gr'CG_yC
Cuts at: 188 554 1246 1494 2198 3694 188
Size: 366 692 248 704 1496 311
Fragments arranged by size:
1496 704 692 366 311 248
BsaWI w'CCGG_w
Cuts at: 1004 1525 2205 2479 2626 3037 1004
Size: 521 680 274 147 411 1784
Fragments arranged by size:
1784 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 478 508 478
Size: 30 3787
BseYI C'CCAG_C
Cuts at: 1240 1998 2577 3073 1240
Size: 758 579 496 1984
Fragments arranged by size:
1984 758 579 496
BsiEI CG_ry'CG
Cuts at: 104 185 389 1357 1403 2613 104
Size: 81 204 968 46 1210 1308
Fragments arranged by size:
1308 1210 968 204 81 46
BsiHKAI G_wGCw'C
Cuts at: 263 377 829 1607 1797 2591 263
Size: 114 452 778 190 794 1489
Fragments arranged by size:
1489 794 778 452 190 114
Bsp1286I G_dGCh'C
Cuts at: 263 377 829 1440 1533 1607 1797 1859 2591
Size: 114 452 611 93 74 190 62 732
Cuts at: 2591 263
Size: 1489
Fragments arranged by size:
1489 732 611 452 190 114 93 74
62
BspEI T'CCGG_A
Cuts at: 3037 3037
Size: 3817
BspHI T'CATG_A
Cuts at: 2993 2993
Size: 3817
BspMI ACCTGCnnnn'nnnn_
Cuts at: 402 423 865 1079 1381 1762 402
Size: 21 442 214 302 381 2457
Fragments arranged by size:
2457 442 381 302 214 21
BsrBI CCG'CTC
Cuts at: 50 326 2107 2161 3647 3812 50
Size: 276 1781 54 1486 165 55
Fragments arranged by size:
1781 1486 276 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1727 1727
Size: 3817
BsrFI r'CCGG_y
Cuts at: 184 388 1813 1994 2205 184
Size: 204 1425 181 211 1796
Fragments arranged by size:
1796 1425 211 204 181
BssSI C'ACGA_G
Cuts at: 2086 2446 2086
Size: 360 3457
BstAPI GCAn_nnn'nTGC
Cuts at: 228 1192 228
Size: 964 2853
BstBI TT'CG_AA
Cuts at: 110 110
Size: 3817
BstYI r'GATC_y
Cuts at: 1 196 840 1307 1330 1665 1911 2925 3011
Size: 195 644 467 23 335 246 1014 86
Cuts at: 3011 3023 3369 1
Size: 12 346 449
Fragments arranged by size:
1014 644 467 449 346 335 246 195
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 213 213
Size: 3817
BtgI C'CryG_G
Cuts at: 125 173 419 482 125
Size: 48 246 63 3460
Fragments arranged by size:
3460 246 63 48
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1765 1946 2109 1765
Size: 181 163 3473
Fragments arranged by size:
3473 181 163
BtsI GCAGTG_nn'
Cuts at: 1058 1058
Size: 3817
DraI TTT'AAA
Cuts at: 641 814 641
Size: 173 3644
DrdI GACnn_nn'nnGTC
Cuts at: 1521 2381 1521
Size: 860 2957
EaeI y'GGCC_r
Cuts at: 182 386 416 1400 1574 1965 1992 3339 182
Size: 204 30 984 174 391 27 1347 660
Fragments arranged by size:
1347 984 660 391 204 174 30 27
EagI C'GGCC_G
Cuts at: 182 386 1400 182
Size: 204 1014 2599
Fragments arranged by size:
2599 1014 204
EarI CTCTTCn'nnn_
Cuts at: 524 1838 2048 524
Size: 1314 210 2293
Fragments arranged by size:
2293 1314 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 391 676 2023 2335 2481 3627 391
Size: 285 1347 312 146 1146 581
Fragments arranged by size:
1347 1146 581 312 285 146
EcoICRI GAG'CTC
Cuts at: 375 827 375
Size: 452 3365
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 721 1640 2072 2821 3089 721
Size: 919 432 749 268 1449
Fragments arranged by size:
1449 919 749 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 409 409
Size: 3817
EcoO109I rG'GnC_Cy
Cuts at: 534 954 534
Size: 420 3397
EcoRI G'AATT_C
Cuts at: 819 819
Size: 3817
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1834 1834
Size: 3817
FspI TGC'GCA
Cuts at: 1596 1596
Size: 3817
HaeII r_GCGC'y
Cuts at: 191 1249 1497 2521 3500 191
Size: 1058 248 1024 979 508
Fragments arranged by size:
1058 1024 979 508 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 478 510 659 691 1657 1687 1689 1719 478
Size: 32 149 32 966 30 2 30 2576
Fragments arranged by size:
2576 966 149 32 32 30 30 2
HincII GTy'rAC
Cuts at: 854 854
Size: 3817
KasI G'GCGC_C
Cuts at: 187 1245 1493 187
Size: 1058 248 2511
Fragments arranged by size:
2511 1058 248
KpnI G_GTAC'C
Cuts at: 835 835
Size: 3817
MluI A'CGCG_T
Cuts at: 3250 3250
Size: 3817
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2488 2672 2488
Size: 184 3633
MscI TGG'CCA
Cuts at: 418 1576 418
Size: 1158 2659
MslI CAynn'nnrTG
Cuts at: 562 1804 1931 562
Size: 1242 127 2448
Fragments arranged by size:
2448 1242 127
MspA1I CmG'CkG
Cuts at: 175 181 447 585 926 1240 1600 2615 2860
Size: 6 266 138 341 314 360 1015 245
Cuts at: 2860 175
Size: 1132
Fragments arranged by size:
1132 1015 360 341 314 266 245 138
6
NaeI GCC'GGC
Cuts at: 186 1996 186
Size: 1810 2007
NarI GG'CG_CC
Cuts at: 188 1246 1494 188
Size: 1058 248 2511
Fragments arranged by size:
2511 1058 248
NcoI C'CATG_G
Cuts at: 125 419 482 125
Size: 294 63 3460
Fragments arranged by size:
3460 294 63
NgoMIV G'CCGG_C
Cuts at: 184 1994 184
Size: 1810 2007
NruI TCG'CGA
Cuts at: 2178 2178
Size: 3817
NspI r_CATG'y
Cuts at: 493 868 1368 2277 493
Size: 375 500 909 2033
Fragments arranged by size:
2033 909 500 375
PciI A'CATG_T
Cuts at: 2273 2273
Size: 3817
PflMI CCAn_nnn'nTGG
Cuts at: 768 768
Size: 3817
PmeI GTTT'AAAC
Cuts at: 814 814
Size: 3817
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2956 2988 2956
Size: 32 3785
PsiI TTA'TAA
Cuts at: 655 655
Size: 3817
PstI C_TGCA'G
Cuts at: 159 862 1547 159
Size: 703 685 2429
Fragments arranged by size:
2429 703 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3817
PvuII CAG'CTG
Cuts at: 585 1240 1600 585
Size: 655 360 2802
Fragments arranged by size:
2802 655 360
RsrII CG'GwC_CG
Cuts at: 2010 2010
Size: 3817
SacI G_AGCT'C
Cuts at: 377 829 377
Size: 452 3365
SacII CC_GC'GG
Cuts at: 176 176
Size: 3817
SalI G'TCGA_C
Cuts at: 852 852
Size: 3817
SapI GCTCTTCn'nnn_
Cuts at: 1838 2048 1838
Size: 210 3607
SbfI CC_TGCA'GG
Cuts at: 862 862
Size: 3817
ScaI AGT'ACT
Cuts at: 3334 3334
Size: 3817
SexAI A'CCwGG_T
Cuts at: 767 767
Size: 3817
SfcI C'TryA_G
Cuts at: 33 155 858 1543 2538 2729 33
Size: 122 703 685 995 191 1121
Fragments arranged by size:
1121 995 703 685 191 122
SfoI GGC'GCC
Cuts at: 189 1247 1495 189
Size: 1058 248 2511
Fragments arranged by size:
2511 1058 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3817
SmaI CCC'GGG
Cuts at: 194 837 194
Size: 643 3174
SmlI C'TyrA_G
Cuts at: 970 1885 2379 2641 970
Size: 915 494 262 2146
Fragments arranged by size:
2146 915 494 262
SnaBI TAC'GTA
Cuts at: 807 807
Size: 3817
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3817
SphI G_CATG'C
Cuts at: 868 1368 868
Size: 500 3317
StyI C'CwwG_G
Cuts at: 125 269 419 482 949 1303 125
Size: 144 150 63 467 354 2639
Fragments arranged by size:
2639 467 354 150 144 63
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 364 1398 1531 1990 2201 364
Size: 1034 133 459 211 1980
Fragments arranged by size:
1980 1034 459 211 133
TatI w'GTAC_w
Cuts at: 248 3332 248
Size: 3084 733
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 502 534 3334 3366 502
Size: 32 2800 32 953
Fragments arranged by size:
2800 953 32 32
Tth111I GACn'n_nGTC
Cuts at: 1612 1612
Size: 3817
XbaI T'CTAG_A
Cuts at: 846 846
Size: 3817
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1310 1310
Size: 3817
XmaI C'CCGG_G
Cuts at: 192 835 192
Size: 643 3174
ZraI GAC'GTC
Cuts at: 555 555
Size: 3817
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI Bsu36I BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NruI NspI PciI
PflMI PmeI PpiI PsiI PstI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SnaBI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AfeI AflII AhdI AleI AlfI AloI ApaI AscI
AvrII BaeI BbsI BbvCI BcgI BfrBI BglI BmgBI
BmtI BpmI Bpu10I BseRI BsgI BsiWI BsmI BsmBI
BsrGI BssHII BstEII BstXI BstZ17I ClaI CspCI DraIII
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NsiI PacI PasI PfoI PmlI PpuMI PshAI
PspOMI PspXI PsrI SanDI SfiI SgrAI SrfI SspI
StuI SwaI XhoI XmnI