Restriction map for
pF1KSDF0201
(Circular) (Six-base) MAPSORT of: pF1KSDF0201.seq Check: 306 from: 1 to: 5248
pF1KSDF0201 5248 bp
With 182 enzymes: SgfI *
June 20, 2008 10:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1769 1972 1769
Size: 203 5045
AccI GT'mk_AC
Cuts at: 956 1982 2284 956
Size: 1026 302 3920
Fragments arranged by size:
3920 1026 302
Acc65I G'GTAC_C
Cuts at: 2262 2262
Size: 5248
AclI AA'CG_TT
Cuts at: 5088 5088
Size: 5248
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 370 1591 3071 3503 4252 4520 370
Size: 1221 1480 432 749 268 1098
Fragments arranged by size:
1480 1221 1098 749 432 268
AflIII A'CryG_T
Cuts at: 2021 2598 3704 4681 2021
Size: 577 1106 977 2588
Fragments arranged by size:
2588 1106 977 577
AgeI A'CCGG_T
Cuts at: 3636 3636
Size: 5248
AleI CACnn'nnGTG
Cuts at: 1861 2020 1861
Size: 159 5089
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 809 809
Size: 5248
AlwNI CAG_nnn'CTG
Cuts at: 315 928 1273 1951 2551 4120 315
Size: 613 345 678 600 1569 1443
Fragments arranged by size:
1569 1443 678 613 600 345
ApaI G_GGCC'C
Cuts at: 473 622 674 701 719 780 867 937 1006
Size: 149 52 27 18 61 87 70 69
Cuts at: 1006 1042 1111 1183 1247 1374 1400 1553 1598
Size: 36 69 72 64 127 26 153 45
Cuts at: 1598 1640 1649 1684 1703 473
Size: 42 9 35 19 4018
Fragments arranged by size:
4018 153 149 127 87 72 70 69
69 64 61 52 45 42 36 35
27 26 19 18 9
ApaLI G'TGCA_C
Cuts at: 2002 4018 2002
Size: 2016 3232
ApoI r'AATT_y
Cuts at: 1903 2250 4601 4857 1903
Size: 347 2351 256 2294
Fragments arranged by size:
2351 2294 347 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5248
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5248
AvaI C'yCGr_G
Cuts at: 379 386 615 763 1034 1696 1704 1887 2266
Size: 7 229 148 271 662 8 183 379
Cuts at: 2266 379
Size: 3361
Fragments arranged by size:
3361 662 379 271 229 183 148 8
7
BamHI G'GATC_C
Cuts at: 2271 2271
Size: 5248
BanI G'GyrC_C
Cuts at: 652 663 793 1717 1783 2014 2262 2676 2924
Size: 11 130 924 66 231 248 414 248
Cuts at: 2924 2959 652
Size: 35 2941
Fragments arranged by size:
2941 924 414 248 248 231 130 66
35 11
BanII G_rGCy'C
Cuts at: 362 473 515 622 674 701 719 780 867
Size: 111 42 107 52 27 18 61 87
Cuts at: 867 937 1006 1042 1111 1183 1247 1374 1400
Size: 70 69 36 69 72 64 127 26
Cuts at: 1400 1455 1481 1553 1598 1640 1649 1684 1703
Size: 55 26 72 45 42 9 35 19
Cuts at: 1703 2260 3290 362
Size: 557 1030 2320
Fragments arranged by size:
2320 1030 557 127 111 107 87 72
72 70 69 69 64 61 55 52
45 42 42 36 35 27 26 26
19 18 9
BbeI G_GCGC'C
Cuts at: 2680 2928 2680
Size: 248 5000
BbsI GAAGACnn'nnnn_
Cuts at: 143 143
Size: 5248
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1816 1850 1816
Size: 34 5214
BciVI GTATCCnnnnn_n'
Cuts at: 3144 3907 5248 3144
Size: 763 1341 3144
Fragments arranged by size:
3144 1341 763
BclI T'GATC_A
Cuts at: 2766 2766
Size: 5248
BglI GCCn_nnn'nGGC
Cuts at: 226 407 537 617 626 809 1064 1251 1565
Size: 181 130 80 9 183 255 187 314
Cuts at: 1565 1644 1698 1726 1849 1934 1973 226
Size: 79 54 28 123 85 39 3501
Fragments arranged by size:
3501 314 255 187 183 181 130 123
85 80 79 54 39 28 9
BglII A'GATC_T
Cuts at: 2761 4800 2761
Size: 2039 3209
BlpI GC'TnA_GC
Cuts at: 2358 2358
Size: 5248
Bme1580I G_kGCm'C
Cuts at: 473 622 657 674 701 719 780 798 867
Size: 149 35 17 27 18 61 18 69
Cuts at: 867 937 1006 1042 1111 1183 1247 1374 1400
Size: 70 69 36 69 72 64 127 26
Cuts at: 1400 1553 1598 1640 1649 1684 1703 1722 2006
Size: 153 45 42 9 35 19 19 284
Cuts at: 2006 2017 2871 2964 4022 473
Size: 11 854 93 1058 1699
Fragments arranged by size:
1699 1058 854 284 153 149 127 93
72 70 69 69 69 64 61 45
42 36 35 35 27 26 19 19
18 18 17 11 9
BmgBI CAC'GTC
Cuts at: 1240 2007 1240
Size: 767 4481
BmrI ACTGGGnnnn_n'
Cuts at: 238 1403 2458 2539 2874 5020 238
Size: 1165 1055 81 335 2146 466
Fragments arranged by size:
2146 1165 1055 466 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3613 3613
Size: 5248
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 917 971 1295 1451 1510 1622 2104 917
Size: 54 324 156 59 112 482 4061
Fragments arranged by size:
4061 482 324 156 112 59 54
Bpu10I CC'TnA_GC
Cuts at: 831 831
Size: 5248
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 721 2422 3301 3795 4093 721
Size: 1701 879 494 298 1876
Fragments arranged by size:
1876 1701 879 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 4952 4952
Size: 5248
BsaAI yAC'GTr
Cuts at: 2238 2599 2238
Size: 361 4887
BsaBI GATnn'nnATC
Cuts at: 6 2760 4799 6
Size: 2754 2039 455
Fragments arranged by size:
2754 2039 455
BsaHI Gr'CG_yC
Cuts at: 202 1979 2677 2925 3629 5125 202
Size: 1777 698 248 704 1496 325
Fragments arranged by size:
1777 1496 704 698 325 248
BsaWI w'CCGG_w
Cuts at: 1910 2435 2956 3636 3910 4057 4468 1910
Size: 525 521 680 274 147 411 2690
Fragments arranged by size:
2690 680 525 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1668 1698 2185 2215 1668
Size: 30 487 30 4701
Fragments arranged by size:
4701 487 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 2193 2193
Size: 5248
BseYI C'CCAG_C
Cuts at: 129 183 466 1110 1114 1330 1633 1791 1821
Size: 54 283 644 4 216 303 158 30
Cuts at: 1821 1944 2671 3429 4008 4504 129
Size: 123 727 758 579 496 873
Fragments arranged by size:
873 758 727 644 579 496 303 283
216 158 123 54 30 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2170 2170
Size: 5248
BsiEI CG_ry'CG
Cuts at: 104 2788 2834 4044 104
Size: 2684 46 1210 1308
Fragments arranged by size:
2684 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 1868 2006 2120 2260 3038 3228 4022 1868
Size: 138 114 140 778 190 794 3094
Fragments arranged by size:
3094 794 778 190 140 138 114
BsmI GAATG_Cn'
Cuts at: 1298 1298
Size: 5248
Bsp1286I G_dGCh'C
Cuts at: 362 473 515 622 657 674 701 719 780
Size: 111 42 107 35 17 27 18 61
Cuts at: 780 798 867 937 1006 1042 1111 1183 1247
Size: 18 69 70 69 36 69 72 64
Cuts at: 1247 1374 1400 1455 1481 1553 1598 1640 1649
Size: 127 26 55 26 72 45 42 9
Cuts at: 1649 1684 1703 1722 1868 2006 2017 2120 2260
Size: 35 19 19 146 138 11 103 140
Cuts at: 2260 2871 2964 3038 3228 3290 4022 362
Size: 611 93 74 190 62 732 1588
Fragments arranged by size:
1588 732 611 190 146 140 138 127
111 107 103 93 74 72 72 70
69 69 69 64 62 61 55 45
42 42 36 35 35 27 26 26
19 19 18 18 17 11 9
BspEI T'CCGG_A
Cuts at: 4468 4468
Size: 5248
BspHI T'CATG_A
Cuts at: 4424 4424
Size: 5248
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1769 1972 2296 2510 2812 3193 1769
Size: 203 324 214 302 381 3824
Fragments arranged by size:
3824 381 324 302 214 203
BsrBI CCG'CTC
Cuts at: 50 3538 3592 5078 5243 50
Size: 3488 54 1486 165 55
Fragments arranged by size:
3488 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3158 3158
Size: 5248
BsrFI r'CCGG_y
Cuts at: 220 1848 2052 3244 3425 3636 220
Size: 1628 204 1192 181 211 1832
Fragments arranged by size:
1832 1628 1192 211 204 181
BsrGI T'GTAC_A
Cuts at: 2036 2036
Size: 5248
BssSI C'ACGA_G
Cuts at: 3517 3877 3517
Size: 360 4888
BstAPI GCAn_nnn'nTGC
Cuts at: 2623 2623
Size: 5248
BstBI TT'CG_AA
Cuts at: 110 110
Size: 5248
BstEII G'GTnAC_C
Cuts at: 1306 1306
Size: 5248
BstXI CCAn_nnnn'nTGG
Cuts at: 2104 2104
Size: 5248
BstYI r'GATC_y
Cuts at: 1 2271 2738 2761 3096 3342 4356 4442 4454
Size: 2270 467 23 335 246 1014 86 12
Cuts at: 4454 4800 1
Size: 346 449
Fragments arranged by size:
2270 1014 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 624 1256 1498 1509 624
Size: 632 242 11 4363
Fragments arranged by size:
4363 632 242 11
BtgI C'CryG_G
Cuts at: 196 447 1374 1640 2186 196
Size: 251 927 266 546 3258
Fragments arranged by size:
3258 927 546 266 251
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1731 3196 3377 3540 1731
Size: 1465 181 163 3439
Fragments arranged by size:
3439 1465 181 163
BtsI GCAGTG_nn'
Cuts at: 1856 2489 1856
Size: 633 4615
DraI TTT'AAA
Cuts at: 2245 2245
Size: 5248
DrdI GACnn_nn'nnGTC
Cuts at: 147 1529 2952 3812 147
Size: 1382 1423 860 1583
Fragments arranged by size:
1583 1423 1382 860
EaeI y'GGCC_r
Cuts at: 1926 2054 2831 3005 3396 3423 4770 1926
Size: 128 777 174 391 27 1347 2404
Fragments arranged by size:
2404 1347 777 391 174 128 27
EagI C'GGCC_G
Cuts at: 2831 2831
Size: 5248
EarI CTCTTCn'nnn_
Cuts at: 3269 3479 3269
Size: 210 5038
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3454 3766 3912 5058 3454
Size: 312 146 1146 3644
Fragments arranged by size:
3644 1146 312 146
EcoICRI GAG'CTC
Cuts at: 2258 2258
Size: 5248
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 370 917 971 1295 1451 1510 1591 1622 2104
Size: 547 54 324 156 59 81 31 482
Cuts at: 2104 3071 3503 4252 4520 370
Size: 967 432 749 268 1098
Fragments arranged by size:
1098 967 749 547 482 432 324 268
156 81 59 54 31
EcoNI CCTnn'n_nnAGG
Cuts at: 2095 2095
Size: 5248
EcoO109I rG'GnC_Cy
Cuts at: 274 391 470 582 592 601 619 670 671
Size: 117 79 112 10 9 18 51 1
Cuts at: 671 697 698 716 776 777 863 864 907
Size: 26 1 18 60 1 86 1 43
Cuts at: 907 943 1002 1031 1038 1039 1057 1090 1107
Size: 36 59 29 7 1 18 33 17
Cuts at: 1107 1119 1179 1198 1244 1270 1279 1315 1344
Size: 12 60 19 46 26 9 36 29
Cuts at: 1344 1370 1504 1550 1594 1595 1645 1646 1656
Size: 26 134 46 44 1 50 1 10
Cuts at: 1656 1681 1690 1700 1708 1727 2385 274
Size: 25 9 10 8 19 658 3137
Fragments arranged by size:
3137 658 134 117 112 86 79 60
60 59 51 50 46 46 44 43
36 36 33 29 29 26 26 26
25 19 19 18 18 18 17 12
10 10 10 9 9 9 8 7
1 1 1 1 1 1 1
EcoRI G'AATT_C
Cuts at: 2250 2250
Size: 5248
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3265 3265
Size: 5248
FspI TGC'GCA
Cuts at: 3027 3027
Size: 5248
HaeII r_GCGC'y
Cuts at: 2680 2928 3952 4931 2680
Size: 248 1024 979 2997
Fragments arranged by size:
2997 1024 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2086 2118 2207 2239 3088 3118 3120 3150 2086
Size: 32 89 32 849 30 2 30 4184
Fragments arranged by size:
4184 849 89 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2285 2285
Size: 5248
KasI G'GCGC_C
Cuts at: 2676 2924 2676
Size: 248 5000
KpnI G_GTAC'C
Cuts at: 2266 2266
Size: 5248
MluI A'CGCG_T
Cuts at: 4681 4681
Size: 5248
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2141 3919 4103 2141
Size: 1778 184 3286
Fragments arranged by size:
3286 1778 184
MscI TGG'CCA
Cuts at: 1928 3007 1928
Size: 1079 4169
MslI CAynn'nnrTG
Cuts at: 1861 1897 2020 3235 3362 1861
Size: 36 123 1215 127 3747
Fragments arranged by size:
3747 1215 127 123 36
MspA1I CmG'CkG
Cuts at: 198 333 826 1114 1537 1934 2124 2357 2671
Size: 135 493 288 423 397 190 233 314
Cuts at: 2671 3031 4046 4291 198
Size: 360 1015 245 1155
Fragments arranged by size:
1155 1015 493 423 397 360 314 288
245 233 190 135
NaeI GCC'GGC
Cuts at: 2054 3427 2054
Size: 1373 3875
NarI GG'CG_CC
Cuts at: 2677 2925 2677
Size: 248 5000
NcoI C'CATG_G
Cuts at: 447 1374 447
Size: 927 4321
NgoMIV G'CCGG_C
Cuts at: 2052 3425 2052
Size: 1373 3875
NruI TCG'CGA
Cuts at: 3609 3609
Size: 5248
NspI r_CATG'y
Cuts at: 1896 2299 2799 3708 1896
Size: 403 500 909 3436
Fragments arranged by size:
3436 909 500 403
PasI CC'CwG_GG
Cuts at: 370 508 702 781 911 973 1045 1249 1455
Size: 138 194 79 130 62 72 204 206
Cuts at: 1455 1483 1651 1723 370
Size: 28 168 72 3895
Fragments arranged by size:
3895 206 204 194 168 138 130 79
72 72 62 28
PciI A'CATG_T
Cuts at: 3704 3704
Size: 5248
PflMI CCAn_nnn'nTGG
Cuts at: 315 1816 1951 2024 315
Size: 1501 135 73 3539
Fragments arranged by size:
3539 1501 135 73
PfoI T'CCnGG_A
Cuts at: 484 894 948 484
Size: 410 54 4784
Fragments arranged by size:
4784 410 54
PmeI GTTT'AAAC
Cuts at: 2245 2245
Size: 5248
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4387 4419 4387
Size: 32 5216
PpuMI rG'GwC_Cy
Cuts at: 274 391 592 601 943 1031 1119 1198 1279
Size: 117 201 9 342 88 88 79 81
Cuts at: 1279 1344 1504 1656 1708 274
Size: 65 160 152 52 3814
Fragments arranged by size:
3814 342 201 160 152 117 88 88
81 79 65 52 9
PspOMI G'GGCC_C
Cuts at: 469 618 670 697 715 776 863 933 1002
Size: 149 52 27 18 61 87 70 69
Cuts at: 1002 1038 1107 1179 1243 1370 1396 1549 1594
Size: 36 69 72 64 127 26 153 45
Cuts at: 1594 1636 1645 1680 1699 469
Size: 42 9 35 19 4018
Fragments arranged by size:
4018 153 149 127 87 72 70 69
69 64 61 52 45 42 36 35
27 26 19 18 9
PstI C_TGCA'G
Cuts at: 1224 2293 2978 1224
Size: 1069 685 3494
Fragments arranged by size:
3494 1069 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5248
PvuII CAG'CTG
Cuts at: 826 1114 1537 2124 2671 3031 826
Size: 288 423 587 547 360 3043
Fragments arranged by size:
3043 587 547 423 360 288
RsrII CG'GwC_CG
Cuts at: 3441 3441
Size: 5248
SacI G_AGCT'C
Cuts at: 2260 2260
Size: 5248
SacII CC_GC'GG
Cuts at: 199 199
Size: 5248
SalI G'TCGA_C
Cuts at: 2283 2283
Size: 5248
SanDI GG'GwC_CC
Cuts at: 391 592 1031 1119 1198 1344 1504 1656 1708
Size: 201 439 88 79 146 160 152 52
Cuts at: 1708 391
Size: 3931
Fragments arranged by size:
3931 439 201 160 152 146 88 79
52
SapI GCTCTTCn'nnn_
Cuts at: 3269 3479 3269
Size: 210 5038
SbfI CC_TGCA'GG
Cuts at: 2293 2293
Size: 5248
ScaI AGT'ACT
Cuts at: 4765 4765
Size: 5248
SexAI A'CCwGG_T
Cuts at: 648 840 2138 648
Size: 192 1298 3758
Fragments arranged by size:
3758 1298 192
SfcI C'TryA_G
Cuts at: 33 1220 2289 2974 3969 4160 33
Size: 1187 1069 685 995 191 1121
Fragments arranged by size:
1187 1121 1069 995 685 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 617 809 1251 1565 1644 1698 617
Size: 192 442 314 79 54 4167
Fragments arranged by size:
4167 442 314 192 79 54
SfoI GGC'GCC
Cuts at: 2678 2926 2678
Size: 248 5000
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5248
SmaI CCC'GGG
Cuts at: 381 617 765 1036 1698 1706 2268 381
Size: 236 148 271 662 8 562 3361
Fragments arranged by size:
3361 662 562 271 236 148 8
SmlI C'TyrA_G
Cuts at: 736 2401 3316 3810 4072 736
Size: 1665 915 494 262 1912
Fragments arranged by size:
1912 1665 915 494 262
SnaBI TAC'GTA
Cuts at: 2238 2238
Size: 5248
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5248
SphI G_CATG'C
Cuts at: 1896 2299 2799 1896
Size: 403 500 4345
Fragments arranged by size:
4345 500 403
SrfI GCCC'GGGC
Cuts at: 381 765 381
Size: 384 4864
StuI AGG'CCT
Cuts at: 734 1559 1586 734
Size: 825 27 4396
Fragments arranged by size:
4396 825 27
StyI C'CwwG_G
Cuts at: 447 657 1094 1374 1400 1554 2380 2734 447
Size: 210 437 280 26 154 826 354 2961
Fragments arranged by size:
2961 826 437 354 280 210 154 26
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2016 2136 2829 2962 3421 3632 2016
Size: 120 693 133 459 211 3632
Fragments arranged by size:
3632 693 459 211 133 120
TatI w'GTAC_w
Cuts at: 251 2036 2075 2192 4763 251
Size: 1785 39 117 2571 736
Fragments arranged by size:
2571 1785 736 117 39
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1427 1459 1668 1700 4765 4797 1427
Size: 32 209 32 3065 32 1878
Fragments arranged by size:
3065 1878 209 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2140 3043 2140
Size: 903 4345
XbaI T'CTAG_A
Cuts at: 2277 2277
Size: 5248
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 454 709 1318 1381 1534 1773 2025 2104 2741
Size: 255 609 63 153 239 252 79 637
Cuts at: 2741 454
Size: 2961
Fragments arranged by size:
2961 637 609 255 252 239 153 79
63
XmaI C'CCGG_G
Cuts at: 379 615 763 1034 1696 1704 2266 379
Size: 236 148 271 662 8 562 3361
Fragments arranged by size:
3361 662 562 271 236 148 8
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NruI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PspOMI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SgfI SmaI SmlI SnaBI
SpeI SphI SrfI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AloI AscI AvrII BaeI
BbvCI BfrBI BmtI BsiWI BsmBI BssHII BstZ17I ClaI
CspCI DraIII EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NsiI PacI PmlI PshAI PsiI
PspXI PsrI SgrAI SspI SwaI XhoI XmnI ZraI