Restriction map for
pF1KSDF0261
(Circular) (Six-base) MAPSORT of: pF1KSDF0261.seq Check: 2900 from: 1 to: 7930
pF1KSDF0261 7930 bp
With 182 enzymes: SgfI *
June 20, 2008 10:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1107 1722 4388 4809 1107
Size: 615 2666 421 4228
Fragments arranged by size:
4228 2666 615 421
AatII G_ACGT'C
Cuts at: 3809 3809
Size: 7930
AccI GT'mk_AC
Cuts at: 3914 4966 3914
Size: 1052 6878
Acc65I G'GTAC_C
Cuts at: 4944 4944
Size: 7930
AclI AA'CG_TT
Cuts at: 7770 7770
Size: 7930
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 629 1715 2152 2404 3260 4129 4882 5753 6185
Size: 1086 437 252 856 869 753 871 432
Cuts at: 6185 6934 7202 629
Size: 749 268 1357
Fragments arranged by size:
1357 1086 871 869 856 753 749 437
432 268 252
AflIII A'CryG_T
Cuts at: 4366 5280 6386 7363 4366
Size: 914 1106 977 4933
Fragments arranged by size:
4933 1106 977 914
AgeI A'CCGG_T
Cuts at: 6318 6318
Size: 7930
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3634 3634
Size: 7930
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3941 3973 3941
Size: 32 7898
AlwNI CAG_nnn'CTG
Cuts at: 1789 3243 3325 3468 3966 4860 5233 6802 1789
Size: 1454 82 143 498 894 373 1569 2917
Fragments arranged by size:
2917 1569 1454 894 498 373 143 82
ApaLI G'TGCA_C
Cuts at: 4850 6700 4850
Size: 1850 6080
ApoI r'AATT_y
Cuts at: 181 577 3661 4932 7283 7539 181
Size: 396 3084 1271 2351 256 572
Fragments arranged by size:
3084 2351 1271 572 396 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 7930
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7930
AvaI C'yCGr_G
Cuts at: 1423 2720 3490 4418 4694 4948 1423
Size: 1297 770 928 276 254 4405
Fragments arranged by size:
4405 1297 928 770 276 254
AvrII C'CTAG_G
Cuts at: 2415 2415
Size: 7930
BamHI G'GATC_C
Cuts at: 1585 2285 4953 1585
Size: 700 2668 4562
Fragments arranged by size:
4562 2668 700
BanI G'GyrC_C
Cuts at: 1119 1230 1396 1915 4549 4944 5358 5606 5641
Size: 111 166 519 2634 395 414 248 35
Cuts at: 5641 1119
Size: 3408
Fragments arranged by size:
3408 2634 519 414 395 248 166 111
35
BanII G_rGCy'C
Cuts at: 389 497 788 1004 1490 1528 2960 3274 4942
Size: 108 291 216 486 38 1432 314 1668
Cuts at: 4942 5972 389
Size: 1030 2347
Fragments arranged by size:
2347 1668 1432 1030 486 314 291 216
108 38
BbeI G_GCGC'C
Cuts at: 4553 5362 5610 4553
Size: 809 248 6873
Fragments arranged by size:
6873 809 248
BbsI GAAGACnn'nnnn_
Cuts at: 3644 3644
Size: 7930
BbvCI CC'TCA_GC
Cuts at: 134 410 1448 1594 3044 134
Size: 276 1038 146 1450 5020
Fragments arranged by size:
5020 1450 1038 276 146
BciVI GTATCCnnnnn_n'
Cuts at: 5826 6589 7930 5826
Size: 763 1341 5826
Fragments arranged by size:
5826 1341 763
BclI T'GATC_A
Cuts at: 5448 5448
Size: 7930
BglI GCCn_nnn'nGGC
Cuts at: 461 2025 3147 3239 4153 461
Size: 1564 1122 92 914 4238
Fragments arranged by size:
4238 1564 1122 914 92
BglII A'GATC_T
Cuts at: 5443 7482 5443
Size: 2039 5891
BlpI GC'TnA_GC
Cuts at: 5040 5040
Size: 7930
Bme1580I G_kGCm'C
Cuts at: 1800 4854 5553 5646 6704 1800
Size: 3054 699 93 1058 3026
Fragments arranged by size:
3054 3026 1058 699 93
BmgBI CAC'GTC
Cuts at: 4731 4731
Size: 7930
BmrI ACTGGGnnnn_n'
Cuts at: 444 1016 1531 1679 1732 5140 5221 5556 7702
Size: 572 515 148 53 3408 81 335 2146
Cuts at: 7702 444
Size: 672
Fragments arranged by size:
3408 2146 672 572 515 335 148 81
53
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 657 1082 6295 657
Size: 425 5213 2292
Fragments arranged by size:
5213 2292 425
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 835 1766 1958 2944 3142 3249 3490 3552 4030
Size: 931 192 986 198 107 241 62 478
Cuts at: 4030 4081 4189 4666 4738 4789 835
Size: 51 108 477 72 51 3976
Fragments arranged by size:
3976 986 931 478 477 241 198 192
108 107 72 62 51 51
Bpu10I CC'TnA_GC
Cuts at: 134 410 1067 1448 1594 2528 3044 4237 134
Size: 276 657 381 146 934 516 1193 3827
Fragments arranged by size:
3827 1193 934 657 516 381 276 146
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2129 2607 4027 5104 5983 6477 6775 2129
Size: 478 1420 1077 879 494 298 3284
Fragments arranged by size:
3284 1420 1077 879 494 478 298
BsaI GGTCTCn'nnnn_
Cuts at: 228 694 2163 2208 2997 3534 7634 228
Size: 466 1469 45 789 537 4100 524
Fragments arranged by size:
4100 1469 789 537 524 466 45
BsaAI yAC'GTr
Cuts at: 1777 4920 5281 1777
Size: 3143 361 4426
Fragments arranged by size:
4426 3143 361
BsaBI GATnn'nnATC
Cuts at: 6 5442 7481 6
Size: 5436 2039 455
Fragments arranged by size:
5436 2039 455
BsaHI Gr'CG_yC
Cuts at: 3130 3806 4550 5359 5607 6311 7807 3130
Size: 676 744 809 248 704 1496 3253
Fragments arranged by size:
3253 1496 809 744 704 676 248
BsaWI w'CCGG_w
Cuts at: 426 2952 5117 5638 6318 6592 6739 7150 426
Size: 2526 2165 521 680 274 147 411 1206
Fragments arranged by size:
2526 2165 1206 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 468 498 1872 1902 2220 2250 3115 3145 3463
Size: 30 1374 30 318 30 865 30 318
Cuts at: 3463 3493 4079 4109 468
Size: 30 586 30 4289
Fragments arranged by size:
4289 1374 865 586 318 318 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 149 151 225 583 778 832 1320 1333 1364
Size: 2 74 358 195 54 488 13 31
Cuts at: 1364 1555 1646 1670 2297 2334 2393 2721 3390
Size: 191 91 24 627 37 59 328 669
Cuts at: 3390 3522 4119 4150 4237 4452 4645 149
Size: 132 597 31 87 215 193 3434
Fragments arranged by size:
3434 669 627 597 488 358 328 215
195 193 191 132 91 87 74 59
54 37 31 31 24 13 2
BseYI C'CCAG_C
Cuts at: 143 1284 1899 2173 2185 3106 3404 5353 6111
Size: 1141 615 274 12 921 298 1949 758
Cuts at: 6111 6690 7186 143
Size: 579 496 887
Fragments arranged by size:
1949 1141 921 887 758 615 579 496
298 274 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1757 3100 4366 4834 1757
Size: 1343 1266 468 4853
Fragments arranged by size:
4853 1343 1266 468
BsiEI CG_ry'CG
Cuts at: 104 5470 5516 6726 104
Size: 5366 46 1210 1308
Fragments arranged by size:
5366 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 497 1501 1782 2719 2960 3770 4854 4942 5720
Size: 1004 281 937 241 810 1084 88 778
Cuts at: 5720 5910 6704 497
Size: 190 794 1723
Fragments arranged by size:
1723 1084 1004 937 810 794 778 281
241 190 88
BsmBI CGTCTCn'nnnn_
Cuts at: 337 3797 337
Size: 3460 4470
Bsp1286I G_dGCh'C
Cuts at: 389 497 788 1004 1490 1501 1528 1782 1800
Size: 108 291 216 486 11 27 254 18
Cuts at: 1800 2719 2960 3274 3770 4854 4942 5553 5646
Size: 919 241 314 496 1084 88 611 93
Cuts at: 5646 5720 5910 5972 6704 389
Size: 74 190 62 732 1615
Fragments arranged by size:
1615 1084 919 732 611 496 486 314
291 254 241 216 190 108 93 88
74 62 27 18 11
BspEI T'CCGG_A
Cuts at: 426 2952 7150 426
Size: 2526 4198 1206
Fragments arranged by size:
4198 2526 1206
BspHI T'CATG_A
Cuts at: 1409 7106 1409
Size: 5697 2233
BspMI ACCTGCnnnn'nnnn_
Cuts at: 906 1107 1722 2046 2772 3554 3701 3926 4388
Size: 201 615 324 726 782 147 225 462
Cuts at: 4388 4809 4978 5192 5494 5875 906
Size: 421 169 214 302 381 2961
Fragments arranged by size:
2961 782 726 615 462 421 381 324
302 225 214 201 169 147
BsrBI CCG'CTC
Cuts at: 50 1351 4275 6220 6274 7760 7925 50
Size: 1301 2924 1945 54 1486 165 55
Fragments arranged by size:
2924 1945 1486 1301 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2683 5840 2683
Size: 3157 4773
BsrFI r'CCGG_y
Cuts at: 3233 5926 6107 6318 3233
Size: 2693 181 211 4845
Fragments arranged by size:
4845 2693 211 181
BssSI C'ACGA_G
Cuts at: 2363 6199 6559 2363
Size: 3836 360 3734
Fragments arranged by size:
3836 3734 360
BstAPI GCAn_nnn'nTGC
Cuts at: 3243 5305 3243
Size: 2062 5868
BstBI TT'CG_AA
Cuts at: 110 110
Size: 7930
BstEII G'GTnAC_C
Cuts at: 3071 3071
Size: 7930
BstXI CCAn_nnnn'nTGG
Cuts at: 497 552 1268 497
Size: 55 716 7159
Fragments arranged by size:
7159 716 55
BstYI r'GATC_y
Cuts at: 1 610 1585 2285 2627 3449 4604 4953 5420
Size: 609 975 700 342 822 1155 349 467
Cuts at: 5420 5443 5778 6024 7038 7124 7136 7482 1
Size: 23 335 246 1014 86 12 346 449
Fragments arranged by size:
1155 1014 975 822 700 609 467 449
349 346 342 335 246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 197 761 1364 3005 3266 197
Size: 564 603 1641 261 4861
Fragments arranged by size:
4861 1641 603 564 261
BtgI C'CryG_G
Cuts at: 125 2270 2339 3296 3505 3560 3596 125
Size: 2145 69 957 209 55 36 4459
Fragments arranged by size:
4459 2145 957 209 69 55 36
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 5878 6059 6222 5878
Size: 181 163 7586
Fragments arranged by size:
7586 181 163
BtsI GCAGTG_nn'
Cuts at: 1298 3186 4761 5171 1298
Size: 1888 1575 410 4057
Fragments arranged by size:
4057 1888 1575 410
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 2666 2701 2666
Size: 35 7895
DraI TTT'AAA
Cuts at: 4927 4927
Size: 7930
DrdI GACnn_nn'nnGTC
Cuts at: 1542 2177 2768 2782 5634 6494 1542
Size: 635 591 14 2852 860 2978
Fragments arranged by size:
2978 2852 860 635 591 14
EaeI y'GGCC_r
Cuts at: 583 2355 2477 2825 4616 5513 5687 6078 6105
Size: 1772 122 348 1791 897 174 391 27
Cuts at: 6105 7452 583
Size: 1347 1061
Fragments arranged by size:
1791 1772 1347 1061 897 391 348 174
122 27
EagI C'GGCC_G
Cuts at: 5513 5513
Size: 7930
EarI CTCTTCn'nnn_
Cuts at: 560 626 648 680 743 1091 1099 1121 1293
Size: 66 22 32 63 348 8 22 172
Cuts at: 1293 1310 2934 3279 3784 3830 4105 4393 4573
Size: 17 1624 345 505 46 275 288 180
Cuts at: 4573 5951 6161 560
Size: 1378 210 2329
Fragments arranged by size:
2329 1624 1378 505 348 345 288 275
210 180 172 66 63 46 32 22
22 17 8
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1153 3895 6136 6448 6594 7740 1153
Size: 2742 2241 312 146 1146 1343
Fragments arranged by size:
2742 2241 1343 1146 312 146
EcoICRI GAG'CTC
Cuts at: 495 2958 4940 495
Size: 2463 1982 3485
Fragments arranged by size:
3485 2463 1982
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 629 835 1715 1766 1958 2152 2404 2944 3142
Size: 206 880 51 192 194 252 540 198
Cuts at: 3142 3249 3260 3490 3552 4030 4081 4129 4189
Size: 107 11 230 62 478 51 48 60
Cuts at: 4189 4666 4738 4789 4882 5753 6185 6934 7202
Size: 477 72 51 93 871 432 749 268
Cuts at: 7202 629
Size: 1357
Fragments arranged by size:
1357 880 871 749 540 478 477 432
268 252 230 206 198 194 192 107
93 72 62 60 51 51 51 48
11
EcoNI CCTnn'n_nnAGG
Cuts at: 1749 3047 3124 3442 3583 1749
Size: 1298 77 318 141 6096
Fragments arranged by size:
6096 1298 318 141 77
EcoO109I rG'GnC_Cy
Cuts at: 258 1733 1895 2027 2965 3009 3240 3313 3522
Size: 1475 162 132 938 44 231 73 209
Cuts at: 3522 5067 258
Size: 1545 3121
Fragments arranged by size:
3121 1545 1475 938 231 209 162 132
73 44
EcoRI G'AATT_C
Cuts at: 181 4932 181
Size: 4751 3179
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2467 2783 5947 2467
Size: 316 3164 4450
Fragments arranged by size:
4450 3164 316
FspI TGC'GCA
Cuts at: 3247 3604 3697 5709 3247
Size: 357 93 2012 5468
Fragments arranged by size:
5468 2012 357 93
HaeII r_GCGC'y
Cuts at: 3815 4035 4553 5362 5610 6634 7613 3815
Size: 220 518 809 248 1024 979 4132
Fragments arranged by size:
4132 1024 979 809 518 248 220
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 247 279 468 500 507 539 1441 1473 1804
Size: 32 189 32 7 32 902 32 331
Cuts at: 1804 1836 1872 1904 3027 3059 3115 3147 3242
Size: 32 36 32 1123 32 56 32 95
Cuts at: 3242 3274 3463 3495 4728 4760 5770 5800 5802
Size: 32 189 32 1233 32 1010 30 2
Cuts at: 5802 5832 247
Size: 30 2345
Fragments arranged by size:
2345 1233 1123 1010 902 331 189 189
95 56 36 32 32 32 32 32
32 32 32 32 32 32 30 30
7 2
HincII GTy'rAC
Cuts at: 4967 4967
Size: 7930
HindIII A'AGCT_T
Cuts at: 4594 4594
Size: 7930
KasI G'GCGC_C
Cuts at: 4549 5358 5606 4549
Size: 809 248 6873
Fragments arranged by size:
6873 809 248
KpnI G_GTAC'C
Cuts at: 4948 4948
Size: 7930
MluI A'CGCG_T
Cuts at: 4366 7363 4366
Size: 2997 4933
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3750 3782 4092 4545 4616 4859 6601 6785 3750
Size: 32 310 453 71 243 1742 184 4895
Fragments arranged by size:
4895 1742 453 310 243 184 71 32
MscI TGG'CCA
Cuts at: 585 2357 2479 4618 5689 585
Size: 1772 122 2139 1071 2826
Fragments arranged by size:
2826 2139 1772 1071 122
MslI CAynn'nnrTG
Cuts at: 2363 3712 5917 6044 2363
Size: 1349 2205 127 4249
Fragments arranged by size:
4249 2205 1349 127
MspA1I CmG'CkG
Cuts at: 1241 2473 3137 3408 3465 3507 3552 3572 4176
Size: 1232 664 271 57 42 45 20 604
Cuts at: 4176 4899 5039 5353 5713 6728 6973 1241
Size: 723 140 314 360 1015 245 2198
Fragments arranged by size:
2198 1232 1015 723 664 604 360 314
271 245 140 57 45 42 20
NaeI GCC'GGC
Cuts at: 3235 6109 3235
Size: 2874 5056
NarI GG'CG_CC
Cuts at: 4550 5359 5607 4550
Size: 809 248 6873
Fragments arranged by size:
6873 809 248
NcoI C'CATG_G
Cuts at: 125 2270 3560 125
Size: 2145 1290 4495
Fragments arranged by size:
4495 2145 1290
NdeI CA'TA_TG
Cuts at: 2732 2732
Size: 7930
NgoMIV G'CCGG_C
Cuts at: 3233 6107 3233
Size: 2874 5056
NruI TCG'CGA
Cuts at: 6291 6291
Size: 7930
NspI r_CATG'y
Cuts at: 2434 4981 5481 6390 2434
Size: 2547 500 909 3974
Fragments arranged by size:
3974 2547 909 500
PasI CC'CwG_GG
Cuts at: 2198 3064 4342 2198
Size: 866 1278 5786
Fragments arranged by size:
5786 1278 866
PciI A'CATG_T
Cuts at: 6386 6386
Size: 7930
PflMI CCAn_nnn'nTGG
Cuts at: 813 2945 4167 813
Size: 2132 1222 4576
Fragments arranged by size:
4576 2132 1222
PfoI T'CCnGG_A
Cuts at: 3309 3989 4181 3309
Size: 680 192 7058
Fragments arranged by size:
7058 680 192
PmeI GTTT'AAAC
Cuts at: 4927 4927
Size: 7930
PmlI CAC'GTG
Cuts at: 1777 1777
Size: 7930
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 275 307 7069 7101 275
Size: 32 6762 32 1104
Fragments arranged by size:
6762 1104 32 32
PpuMI rG'GwC_Cy
Cuts at: 258 2965 3313 258
Size: 2707 348 4875
Fragments arranged by size:
4875 2707 348
PstI C_TGCA'G
Cuts at: 378 699 773 1118 3551 4975 5660 378
Size: 321 74 345 2433 1424 685 2648
Fragments arranged by size:
2648 2433 1424 685 345 321 74
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7930
PvuII CAG'CTG
Cuts at: 3137 3408 4176 4899 5353 5713 3137
Size: 271 768 723 454 360 5354
Fragments arranged by size:
5354 768 723 454 360 271
RsrII CG'GwC_CG
Cuts at: 6123 6123
Size: 7930
SacI G_AGCT'C
Cuts at: 497 2960 4942 497
Size: 2463 1982 3485
Fragments arranged by size:
3485 2463 1982
SacII CC_GC'GG
Cuts at: 3508 3508
Size: 7930
SalI G'TCGA_C
Cuts at: 4965 4965
Size: 7930
SanDI GG'GwC_CC
Cuts at: 2965 3313 2965
Size: 348 7582
SapI GCTCTTCn'nnn_
Cuts at: 680 743 1293 3279 3784 5951 6161 680
Size: 63 550 1986 505 2167 210 2449
Fragments arranged by size:
2449 2167 1986 550 505 210 63
SbfI CC_TGCA'GG
Cuts at: 4975 4975
Size: 7930
ScaI AGT'ACT
Cuts at: 7447 7447
Size: 7930
SexAI A'CCwGG_T
Cuts at: 1979 1979
Size: 7930
SfcI C'TryA_G
Cuts at: 33 374 695 769 1114 2046 2091 2631 3547
Size: 341 321 74 345 932 45 540 916
Cuts at: 3547 4894 4971 5656 6651 6842 33
Size: 1347 77 685 995 191 1121
Fragments arranged by size:
1347 1121 995 932 916 685 540 345
341 321 191 77 74 45
SfoI GGC'GCC
Cuts at: 4551 5360 5608 4551
Size: 809 248 6873
Fragments arranged by size:
6873 809 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7930
SmaI CCC'GGG
Cuts at: 1425 3492 4950 1425
Size: 2067 1458 4405
Fragments arranged by size:
4405 2067 1458
SmlI C'TyrA_G
Cuts at: 2144 2622 4042 5083 5998 6492 6754 2144
Size: 478 1420 1041 915 494 262 3320
Fragments arranged by size:
3320 1420 1041 915 494 478 262
SnaBI TAC'GTA
Cuts at: 4920 4920
Size: 7930
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7930
SphI G_CATG'C
Cuts at: 2434 4981 5481 2434
Size: 2547 500 4883
Fragments arranged by size:
4883 2547 500
SspI AAT'ATT
Cuts at: 4268 4268
Size: 7930
StuI AGG'CCT
Cuts at: 415 2637 3685 415
Size: 2222 1048 4660
Fragments arranged by size:
4660 2222 1048
StyI C'CwwG_G
Cuts at: 125 230 362 1617 1660 1891 2270 2415 3560
Size: 105 132 1255 43 231 379 145 1145
Cuts at: 3560 4322 4613 5062 5416 125
Size: 762 291 449 354 2639
Fragments arranged by size:
2639 1255 1145 762 449 379 354 291
231 145 132 105 43
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2344 5511 5644 6103 6314 2344
Size: 3167 133 459 211 3960
Fragments arranged by size:
3960 3167 459 211 133
TatI w'GTAC_w
Cuts at: 7445 7445
Size: 7930
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2220 2252 3176 3208 7447 7479 2220
Size: 32 924 32 4239 32 2671
Fragments arranged by size:
4239 2671 924 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1437 3880 4021 5725 1437
Size: 2443 141 1704 3642
Fragments arranged by size:
3642 2443 1704 141
XbaI T'CTAG_A
Cuts at: 525 2163 4959 525
Size: 1638 2796 3496
Fragments arranged by size:
3496 2796 1638
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 269 1518 4896 5423 269
Size: 1249 3378 527 2776
Fragments arranged by size:
3378 2776 1249 527
XmaI C'CCGG_G
Cuts at: 1423 3490 4948 1423
Size: 2067 1458 4405
Fragments arranged by size:
4405 2067 1458
XmnI GAAnn'nnTTC
Cuts at: 4595 4595
Size: 7930
ZraI GAC'GTC
Cuts at: 3807 3807
Size: 7930
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI
AvrII BamHI BanI BanII BbeI BbsI BbvCI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NruI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PstI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AflII AhdI AleI ApaI AscI BaeI BcgI
BfrBI BmtI BsiWI BsmI BsrGI BssHII BstZ17I ClaI
DraIII EcoRV FseI FspAI HpaI MfeI NheI NotI
NsiI PacI PshAI PsiI PspOMI PspXI PsrI SfiI
SgrAI SrfI SwaI XhoI