Restriction map for
pF1KSDF0380
(Circular) (Six-base) MAPSORT of: pF1KSDF0380.seq Check: 8793 from: 1 to: 5455
pF1KSDF0380 5455 bp
With 182 enzymes: SgfI *
June 20, 2008 10:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 922 922
Size: 5455
AccI GT'mk_AC
Cuts at: 2491 2491
Size: 5455
Acc65I G'GTAC_C
Cuts at: 2469 2469
Size: 5455
AclI AA'CG_TT
Cuts at: 5295 5295
Size: 5455
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 157 1242 1625 3278 3710 4459 4727 157
Size: 1085 383 1653 432 749 268 885
Fragments arranged by size:
1653 1085 885 749 432 383 268
AflIII A'CryG_T
Cuts at: 2805 3911 4888 2805
Size: 1106 977 3372
Fragments arranged by size:
3372 1106 977
AgeI A'CCGG_T
Cuts at: 3843 3843
Size: 5455
AlwNI CAG_nnn'CTG
Cuts at: 2274 2758 4327 2274
Size: 484 1569 3402
Fragments arranged by size:
3402 1569 484
ApaI G_GGCC'C
Cuts at: 263 393 263
Size: 130 5325
ApaLI G'TGCA_C
Cuts at: 4225 4225
Size: 5455
ApoI r'AATT_y
Cuts at: 1540 2457 4808 5064 1540
Size: 917 2351 256 1931
Fragments arranged by size:
2351 1931 917 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5455
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5455
AvaI C'yCGr_G
Cuts at: 496 1264 1979 2473 496
Size: 768 715 494 3478
Fragments arranged by size:
3478 768 715 494
BamHI G'GATC_C
Cuts at: 1797 2478 1797
Size: 681 4774
BanI G'GyrC_C
Cuts at: 408 849 1863 2102 2469 2883 3131 3166 408
Size: 441 1014 239 367 414 248 35 2697
Fragments arranged by size:
2697 1014 441 414 367 248 239 35
BanII G_rGCy'C
Cuts at: 263 393 992 1373 1980 2467 3497 263
Size: 130 599 381 607 487 1030 2221
Fragments arranged by size:
2221 1030 607 599 487 381 130
BbeI G_GCGC'C
Cuts at: 2106 2887 3135 2106
Size: 781 248 4426
Fragments arranged by size:
4426 781 248
BciVI GTATCCnnnnn_n'
Cuts at: 204 334 3351 4114 5455 204
Size: 130 3017 763 1341 204
Fragments arranged by size:
3017 1341 763 204 130
BclI T'GATC_A
Cuts at: 2973 2973
Size: 5455
BglII A'GATC_T
Cuts at: 642 1071 2968 5007 642
Size: 429 1897 2039 1090
Fragments arranged by size:
2039 1897 1090 429
BlpI GC'TnA_GC
Cuts at: 1805 2080 2565 1805
Size: 275 485 4695
Fragments arranged by size:
4695 485 275
Bme1580I G_kGCm'C
Cuts at: 263 393 1143 1875 1908 3078 3171 4229 263
Size: 130 750 732 33 1170 93 1058 1489
Fragments arranged by size:
1489 1170 1058 750 732 130 93 33
BmgBI CAC'GTC
Cuts at: 207 207
Size: 5455
BmrI ACTGGGnnnn_n'
Cuts at: 257 279 604 836 1862 1895 2324 2665 2746
Size: 22 325 232 1026 33 429 341 81
Cuts at: 2746 3081 5227 257
Size: 335 2146 485
Fragments arranged by size:
2146 1026 485 429 341 335 325 232
81 33 22
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3820 3820
Size: 5455
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 148 1169 1552 1557 1849 2191 2329 148
Size: 1021 383 5 292 342 138 3274
Fragments arranged by size:
3274 1021 383 342 292 138 5
Bpu10I CC'TnA_GC
Cuts at: 1594 1594
Size: 5455
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1894 2629 3508 4002 4300 1894
Size: 735 879 494 298 3049
Fragments arranged by size:
3049 879 735 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 440 1145 1711 5159 440
Size: 705 566 3448 736
Fragments arranged by size:
3448 736 705 566
BsaAI yAC'GTr
Cuts at: 1836 2445 2806 1836
Size: 609 361 4485
Fragments arranged by size:
4485 609 361
BsaBI GATnn'nnATC
Cuts at: 6 652 674 2967 5006 6
Size: 646 22 2293 2039 455
Fragments arranged by size:
2293 2039 646 455 22
BsaHI Gr'CG_yC
Cuts at: 1222 2103 2884 3132 3836 5332 1222
Size: 881 781 248 704 1496 1345
Fragments arranged by size:
1496 1345 881 781 704 248
BsaWI w'CCGG_w
Cuts at: 2432 2642 3163 3843 4117 4264 4675 2432
Size: 210 521 680 274 147 411 3212
Fragments arranged by size:
3212 680 521 411 274 210 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 364 1010 1549 1620 1623 2047 2362 364
Size: 646 539 71 3 424 315 3457
Fragments arranged by size:
3457 646 539 424 315 71 3
BseYI C'CCAG_C
Cuts at: 685 1318 1385 1526 2075 2878 3636 4215 4711
Size: 633 67 141 549 803 758 579 496
Cuts at: 4711 685
Size: 1429
Fragments arranged by size:
1429 803 758 633 579 549 496 141
67
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 827 1537 827
Size: 710 4745
BsiEI CG_ry'CG
Cuts at: 104 2995 3041 4251 104
Size: 2891 46 1210 1308
Fragments arranged by size:
2891 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 1165 1835 1973 2467 3245 3435 4229 1165
Size: 670 138 494 778 190 794 2391
Fragments arranged by size:
2391 794 778 670 494 190 138
BsmI GAATG_Cn'
Cuts at: 936 936
Size: 5455
Bsp1286I G_dGCh'C
Cuts at: 263 393 992 1143 1165 1373 1835 1875 1908
Size: 130 599 151 22 208 462 40 33
Cuts at: 1908 1973 1980 2467 3078 3171 3245 3435 3497
Size: 65 7 487 611 93 74 190 62
Cuts at: 3497 4229 263
Size: 732 1489
Fragments arranged by size:
1489 732 611 599 487 462 208 190
151 130 93 74 65 62 40 33
22 7
BspEI T'CCGG_A
Cuts at: 4675 4675
Size: 5455
BspHI T'CATG_A
Cuts at: 4631 4631
Size: 5455
BspMI ACCTGCnnnn'nnnn_
Cuts at: 815 922 1898 2030 2198 2503 2717 3019 3400
Size: 107 976 132 168 305 214 302 381
Cuts at: 3400 815
Size: 2870
Fragments arranged by size:
2870 976 381 305 302 214 168 132
107
BsrBI CCG'CTC
Cuts at: 50 3745 3799 5285 5450 50
Size: 3695 54 1486 165 55
Fragments arranged by size:
3695 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3365 3365
Size: 5455
BsrFI r'CCGG_y
Cuts at: 3451 3632 3843 3451
Size: 181 211 5063
Fragments arranged by size:
5063 211 181
BsrGI T'GTAC_A
Cuts at: 1133 1133
Size: 5455
BssSI C'ACGA_G
Cuts at: 3724 4084 3724
Size: 360 5095
BstAPI GCAn_nnn'nTGC
Cuts at: 1870 2830 1870
Size: 960 4495
BstBI TT'CG_AA
Cuts at: 110 110
Size: 5455
BstXI CCAn_nnnn'nTGG
Cuts at: 1393 1875 1393
Size: 482 4973
BstYI r'GATC_y
Cuts at: 1 642 1071 1725 1797 1991 2478 2945 2968
Size: 641 429 654 72 194 487 467 23
Cuts at: 2968 3303 3549 4563 4649 4661 5007 1
Size: 335 246 1014 86 12 346 449
Fragments arranged by size:
1014 654 641 487 467 449 429 346
335 246 194 86 72 23 12
Bsu36I CC'TnA_GG
Cuts at: 298 895 298
Size: 597 4858
BtgI C'CryG_G
Cuts at: 1100 1100
Size: 5455
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3403 3584 3747 3403
Size: 181 163 5111
Fragments arranged by size:
5111 181 163
BtsI GCAGTG_nn'
Cuts at: 359 977 1069 2370 2696 359
Size: 618 92 1301 326 3118
Fragments arranged by size:
3118 1301 618 326 92
DraI TTT'AAA
Cuts at: 2452 2452
Size: 5455
DraIII CAC_nnn'GTG
Cuts at: 1870 1903 1870
Size: 33 5422
DrdI GACnn_nn'nnGTC
Cuts at: 876 2365 3159 4019 876
Size: 1489 794 860 2312
Fragments arranged by size:
2312 1489 860 794
EaeI y'GGCC_r
Cuts at: 1286 1490 2390 3038 3212 3603 3630 4977 1286
Size: 204 900 648 174 391 27 1347 1764
Fragments arranged by size:
1764 1347 900 648 391 204 174 27
EagI C'GGCC_G
Cuts at: 3038 3038
Size: 5455
EarI CTCTTCn'nnn_
Cuts at: 881 1004 1028 1261 1378 1626 1641 1647 1674
Size: 123 24 233 117 248 15 6 27
Cuts at: 1674 1727 3476 3686 881
Size: 53 1749 210 2650
Fragments arranged by size:
2650 1749 248 233 210 123 117 53
27 24 15 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3661 3973 4119 5265 3661
Size: 312 146 1146 3851
Fragments arranged by size:
3851 1146 312 146
EcoICRI GAG'CTC
Cuts at: 2465 2465
Size: 5455
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 148 157 1169 1242 1552 1557 1625 1849 2191
Size: 9 1012 73 310 5 68 224 342
Cuts at: 2191 2329 3278 3710 4459 4727 148
Size: 138 949 432 749 268 876
Fragments arranged by size:
1012 949 876 749 432 342 310 268
224 138 73 68 9 5
EcoNI CCTnn'n_nnAGG
Cuts at: 379 1216 2278 379
Size: 837 1062 3556
Fragments arranged by size:
3556 1062 837
EcoO109I rG'GnC_Cy
Cuts at: 259 389 390 2187 2592 259
Size: 130 1 1797 405 3122
Fragments arranged by size:
3122 1797 405 130 1
EcoRI G'AATT_C
Cuts at: 2457 2457
Size: 5455
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 162 910 3472 162
Size: 748 2562 2145
Fragments arranged by size:
2562 2145 748
FspI TGC'GCA
Cuts at: 3234 3234
Size: 5455
HaeII r_GCGC'y
Cuts at: 2106 2887 3135 4159 5138 2106
Size: 781 248 1024 979 2423
Fragments arranged by size:
2423 1024 979 781 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1063 1095 1537 1569 1710 1742 3295 3325 3327
Size: 32 442 32 141 32 1553 30 2
Cuts at: 3327 3357 1063
Size: 30 3161
Fragments arranged by size:
3161 1553 442 141 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 2492 2492
Size: 5455
KasI G'GCGC_C
Cuts at: 2102 2883 3131 2102
Size: 781 248 4426
Fragments arranged by size:
4426 781 248
KpnI G_GTAC'C
Cuts at: 2473 2473
Size: 5455
MluI A'CGCG_T
Cuts at: 4888 4888
Size: 5455
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1445 4126 4310 1445
Size: 2681 184 2590
Fragments arranged by size:
2681 2590 184
MscI TGG'CCA
Cuts at: 1288 1492 3214 1288
Size: 204 1722 3529
Fragments arranged by size:
3529 1722 204
MslI CAynn'nnrTG
Cuts at: 1090 1105 3442 3569 1090
Size: 15 2337 127 2976
Fragments arranged by size:
2976 2337 127 15
MspA1I CmG'CkG
Cuts at: 1530 2079 2564 2878 3238 4253 4498 1530
Size: 549 485 314 360 1015 245 2487
Fragments arranged by size:
2487 1015 549 485 360 314 245
NaeI GCC'GGC
Cuts at: 3634 3634
Size: 5455
NarI GG'CG_CC
Cuts at: 2103 2884 3132 2103
Size: 781 248 4426
Fragments arranged by size:
4426 781 248
NcoI C'CATG_G
Cuts at: 1100 1100
Size: 5455
NgoMIV G'CCGG_C
Cuts at: 3632 3632
Size: 5455
NruI TCG'CGA
Cuts at: 3816 3816
Size: 5455
NspI r_CATG'y
Cuts at: 2506 3006 3915 2506
Size: 500 909 4046
Fragments arranged by size:
4046 909 500
PasI CC'CwG_GG
Cuts at: 1227 1227
Size: 5455
PciI A'CATG_T
Cuts at: 3911 3911
Size: 5455
PflMI CCAn_nnn'nTGG
Cuts at: 616 616
Size: 5455
PfoI T'CCnGG_A
Cuts at: 242 1146 1943 242
Size: 904 797 3754
Fragments arranged by size:
3754 904 797
PmeI GTTT'AAAC
Cuts at: 2452 2452
Size: 5455
PmlI CAC'GTG
Cuts at: 1836 1836
Size: 5455
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4594 4626 4594
Size: 32 5423
PpuMI rG'GwC_Cy
Cuts at: 2187 2187
Size: 5455
PshAI GACnn'nnGTC
Cuts at: 707 707
Size: 5455
PspOMI G'GGCC_C
Cuts at: 259 389 259
Size: 130 5325
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1759 1791 1759
Size: 32 5423
PstI C_TGCA'G
Cuts at: 1240 2195 2500 3185 1240
Size: 955 305 685 3510
Fragments arranged by size:
3510 955 685 305
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5455
PvuII CAG'CTG
Cuts at: 1530 2079 2878 3238 1530
Size: 549 799 360 3747
Fragments arranged by size:
3747 799 549 360
RsrII CG'GwC_CG
Cuts at: 3648 3648
Size: 5455
SacI G_AGCT'C
Cuts at: 2467 2467
Size: 5455
SalI G'TCGA_C
Cuts at: 2490 2490
Size: 5455
SapI GCTCTTCn'nnn_
Cuts at: 1004 1378 3476 3686 1004
Size: 374 2098 210 2773
Fragments arranged by size:
2773 2098 374 210
SbfI CC_TGCA'GG
Cuts at: 2500 2500
Size: 5455
ScaI AGT'ACT
Cuts at: 4972 4972
Size: 5455
SfcI C'TryA_G
Cuts at: 33 599 705 1170 1236 1701 2191 2496 3181
Size: 566 106 465 66 465 490 305 685
Cuts at: 3181 4176 4367 33
Size: 995 191 1121
Fragments arranged by size:
1121 995 685 566 490 465 465 305
191 106 66
SfoI GGC'GCC
Cuts at: 2104 2885 3133 2104
Size: 781 248 4426
Fragments arranged by size:
4426 781 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5455
SmaI CCC'GGG
Cuts at: 2475 2475
Size: 5455
SmlI C'TyrA_G
Cuts at: 496 1909 2608 3523 4017 4279 496
Size: 1413 699 915 494 262 1672
Fragments arranged by size:
1672 1413 915 699 494 262
SnaBI TAC'GTA
Cuts at: 2445 2445
Size: 5455
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5455
SphI G_CATG'C
Cuts at: 2506 3006 2506
Size: 500 4955
StuI AGG'CCT
Cuts at: 488 1522 1559 488
Size: 1034 37 4384
Fragments arranged by size:
4384 1034 37
StyI C'CwwG_G
Cuts at: 308 1100 2587 2941 308
Size: 792 1487 354 2822
Fragments arranged by size:
2822 1487 792 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1881 1914 3036 3169 3628 3839 1881
Size: 33 1122 133 459 211 3497
Fragments arranged by size:
3497 1122 459 211 133 33
TatI w'GTAC_w
Cuts at: 1133 4970 1133
Size: 3837 1618
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1048 1080 4972 5004 1048
Size: 32 3892 32 1499
Fragments arranged by size:
3892 1499 32 32
Tth111I GACn'n_nGTC
Cuts at: 1038 3250 1038
Size: 2212 3243
XbaI T'CTAG_A
Cuts at: 2484 2484
Size: 5455
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2166 2287 2948 2166
Size: 121 661 4673
Fragments arranged by size:
4673 661 121
XhoI C'TCGA_G
Cuts at: 496 496
Size: 5455
XmaI C'CCGG_G
Cuts at: 2473 2473
Size: 5455
XmnI GAAnn'nnTTC
Cuts at: 158 158
Size: 5455
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NruI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PspOMI PsrI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SnaBI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BbsI BbvCI BcgI BfrBI BglI BmtI
BsaXI BsiWI BsmBI BssHII BstEII BstZ17I ClaI CspCI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NsiI PacI PsiI PspXI SacII SanDI SexAI
SfiI SgrAI SrfI SspI SwaI ZraI