Restriction map for
pF1KSDF0390
(Circular) (Six-base) MAPSORT of: pF1KSDF0390.seq Check: 7707 from: 1 to: 3964
pF1KSDF0390 3964 bp
With 182 enzymes: SgfI *
June 20, 2008 10:36 ..
AccI GT'mk_AC
Cuts at: 1000 1000
Size: 3964
Acc65I G'GTAC_C
Cuts at: 978 978
Size: 3964
AclI AA'CG_TT
Cuts at: 3804 3804
Size: 3964
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1787 2219 2968 3236 1787
Size: 432 749 268 2515
Fragments arranged by size:
2515 749 432 268
AflIII A'CryG_T
Cuts at: 1314 2420 3397 1314
Size: 1106 977 1881
Fragments arranged by size:
1881 1106 977
AgeI A'CCGG_T
Cuts at: 2352 2352
Size: 3964
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 901 933 901
Size: 32 3932
AlwNI CAG_nnn'CTG
Cuts at: 162 429 459 531 941 1267 2836 162
Size: 267 30 72 410 326 1569 1290
Fragments arranged by size:
1569 1290 410 326 267 72 30
ApaI G_GGCC'C
Cuts at: 258 258
Size: 3964
ApaLI G'TGCA_C
Cuts at: 2734 2734
Size: 3964
ApoI r'AATT_y
Cuts at: 865 966 3317 3573 865
Size: 101 2351 256 1256
Fragments arranged by size:
2351 1256 256 101
AseI AT'TA_AT
Cuts at: 20 20
Size: 3964
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3964
AvaI C'yCGr_G
Cuts at: 444 541 662 982 444
Size: 97 121 320 3426
Fragments arranged by size:
3426 320 121 97
BamHI G'GATC_C
Cuts at: 987 987
Size: 3964
BanI G'GyrC_C
Cuts at: 275 978 1392 1640 1675 275
Size: 703 414 248 35 2564
Fragments arranged by size:
2564 703 414 248 35
BanII G_rGCy'C
Cuts at: 258 374 976 2006 258
Size: 116 602 1030 2216
Fragments arranged by size:
2216 1030 602 116
BbeI G_GCGC'C
Cuts at: 1396 1644 1396
Size: 248 3716
BciVI GTATCCnnnnn_n'
Cuts at: 1860 2623 3964 1860
Size: 763 1341 1860
Fragments arranged by size:
1860 1341 763
BclI T'GATC_A
Cuts at: 1482 1482
Size: 3964
BglI GCCn_nnn'nGGC
Cuts at: 332 667 332
Size: 335 3629
BglII A'GATC_T
Cuts at: 1477 3516 1477
Size: 2039 1925
BlpI GC'TnA_GC
Cuts at: 1074 1074
Size: 3964
Bme1580I G_kGCm'C
Cuts at: 258 423 762 1587 1680 2738 258
Size: 165 339 825 93 1058 1484
Fragments arranged by size:
1484 1058 825 339 165 93
BmrI ACTGGGnnnn_n'
Cuts at: 1174 1255 1590 3736 1174
Size: 81 335 2146 1402
Fragments arranged by size:
2146 1402 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2329 2329
Size: 3964
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 253 640 253
Size: 387 3577
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1138 2017 2511 2809 1138
Size: 879 494 298 2293
Fragments arranged by size:
2293 879 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 3668 3668
Size: 3964
BsaAI yAC'GTr
Cuts at: 954 1315 954
Size: 361 3603
BsaBI GATnn'nnATC
Cuts at: 6 1476 3515 6
Size: 1470 2039 455
Fragments arranged by size:
2039 1470 455
BsaHI Gr'CG_yC
Cuts at: 389 716 1393 1641 2345 3841 389
Size: 327 677 248 704 1496 512
Fragments arranged by size:
1496 704 677 512 327 248
BsaWI w'CCGG_w
Cuts at: 1151 1672 2352 2626 2773 3184 1151
Size: 521 680 274 147 411 1931
Fragments arranged by size:
1931 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 251 281 814 844 251
Size: 30 533 30 3371
Fragments arranged by size:
3371 533 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 637 822 928 637
Size: 185 106 3673
Fragments arranged by size:
3673 185 106
BseYI C'CCAG_C
Cuts at: 194 229 251 1387 2145 2724 3220 194
Size: 35 22 1136 758 579 496 938
Fragments arranged by size:
1136 938 758 579 496 35 22
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 600 600
Size: 3964
BsiEI CG_ry'CG
Cuts at: 104 1504 1550 2760 104
Size: 1400 46 1210 1308
Fragments arranged by size:
1400 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 976 1754 1944 2738 976
Size: 778 190 794 2202
Fragments arranged by size:
2202 794 778 190
BsmI GAATG_Cn'
Cuts at: 240 240
Size: 3964
Bsp1286I G_dGCh'C
Cuts at: 258 374 423 762 976 1587 1680 1754 1944
Size: 116 49 339 214 611 93 74 190
Cuts at: 1944 2006 2738 258
Size: 62 732 1484
Fragments arranged by size:
1484 732 611 339 214 190 116 93
74 62 49
BspEI T'CCGG_A
Cuts at: 3184 3184
Size: 3964
BspHI T'CATG_A
Cuts at: 3140 3140
Size: 3964
BspMI ACCTGCnnnn'nnnn_
Cuts at: 840 1012 1226 1528 1909 840
Size: 172 214 302 381 2895
Fragments arranged by size:
2895 381 302 214 172
BsrBI CCG'CTC
Cuts at: 50 2254 2308 3794 3959 50
Size: 2204 54 1486 165 55
Fragments arranged by size:
2204 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 603 1874 603
Size: 1271 2693
BsrFI r'CCGG_y
Cuts at: 1960 2141 2352 1960
Size: 181 211 3572
Fragments arranged by size:
3572 211 181
BsrGI T'GTAC_A
Cuts at: 746 746
Size: 3964
BssSI C'ACGA_G
Cuts at: 2233 2593 2233
Size: 360 3604
BstAPI GCAn_nnn'nTGC
Cuts at: 1339 1339
Size: 3964
BstBI TT'CG_AA
Cuts at: 110 110
Size: 3964
BstYI r'GATC_y
Cuts at: 1 141 499 615 987 1454 1477 1812 2058
Size: 140 358 116 372 467 23 335 246
Cuts at: 2058 3072 3158 3170 3516 1
Size: 1014 86 12 346 449
Fragments arranged by size:
1014 467 449 372 358 346 335 246
140 116 86 23 12
BtgI C'CryG_G
Cuts at: 392 404 435 738 392
Size: 12 31 303 3618
Fragments arranged by size:
3618 303 31 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 660 1912 2093 2256 660
Size: 1252 181 163 2368
Fragments arranged by size:
2368 1252 181 163
BtsI GCAGTG_nn'
Cuts at: 136 223 711 783 1205 136
Size: 87 488 72 422 2895
Fragments arranged by size:
2895 488 422 87 72
DraI TTT'AAA
Cuts at: 961 961
Size: 3964
DrdI GACnn_nn'nnGTC
Cuts at: 850 1668 2528 850
Size: 818 860 2286
Fragments arranged by size:
2286 860 818
EaeI y'GGCC_r
Cuts at: 461 533 753 923 1547 1721 2112 2139 3486
Size: 72 220 170 624 174 391 27 1347
Cuts at: 3486 461
Size: 939
Fragments arranged by size:
1347 939 624 391 220 174 170 72
27
EagI C'GGCC_G
Cuts at: 1547 1547
Size: 3964
EarI CTCTTCn'nnn_
Cuts at: 1985 2195 1985
Size: 210 3754
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 463 577 877 2170 2482 2628 3774 463
Size: 114 300 1293 312 146 1146 653
Fragments arranged by size:
1293 1146 653 312 300 146 114
EcoICRI GAG'CTC
Cuts at: 974 974
Size: 3964
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 253 640 1787 2219 2968 3236 253
Size: 387 1147 432 749 268 981
Fragments arranged by size:
1147 981 749 432 387 268
EcoO109I rG'GnC_Cy
Cuts at: 159 255 325 440 456 1101 159
Size: 96 70 115 16 645 3022
Fragments arranged by size:
3022 645 115 96 70 16
EcoRI G'AATT_C
Cuts at: 865 966 865
Size: 101 3863
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1981 1981
Size: 3964
FspI TGC'GCA
Cuts at: 775 1743 775
Size: 968 2996
HaeII r_GCGC'y
Cuts at: 640 1396 1644 2668 3647 640
Size: 756 248 1024 979 957
Fragments arranged by size:
1024 979 957 756 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 600 632 1804 1834 1836 1866 600
Size: 32 1172 30 2 30 2698
Fragments arranged by size:
2698 1172 32 30 30 2
HincII GTy'rAC
Cuts at: 1001 1001
Size: 3964
KasI G'GCGC_C
Cuts at: 1392 1640 1392
Size: 248 3716
KpnI G_GTAC'C
Cuts at: 982 982
Size: 3964
MluI A'CGCG_T
Cuts at: 3397 3397
Size: 3964
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2635 2819 2635
Size: 184 3780
MscI TGG'CCA
Cuts at: 463 535 925 1723 463
Size: 72 390 798 2704
Fragments arranged by size:
2704 798 390 72
MslI CAynn'nnrTG
Cuts at: 1951 2078 1951
Size: 127 3837
MspA1I CmG'CkG
Cuts at: 251 362 437 605 682 740 884 948 1073
Size: 111 75 168 77 58 144 64 125
Cuts at: 1073 1387 1747 2762 3007 251
Size: 314 360 1015 245 1208
Fragments arranged by size:
1208 1015 360 314 245 168 144 125
111 77 75 64 58
NaeI GCC'GGC
Cuts at: 2143 2143
Size: 3964
NarI GG'CG_CC
Cuts at: 1393 1641 1393
Size: 248 3716
NcoI C'CATG_G
Cuts at: 404 404
Size: 3964
NgoMIV G'CCGG_C
Cuts at: 2141 2141
Size: 3964
NruI TCG'CGA
Cuts at: 2325 2325
Size: 3964
NspI r_CATG'y
Cuts at: 1015 1515 2424 1015
Size: 500 909 2555
Fragments arranged by size:
2555 909 500
PciI A'CATG_T
Cuts at: 2420 2420
Size: 3964
PflMI CCAn_nnn'nTGG
Cuts at: 459 941 459
Size: 482 3482
PfoI T'CCnGG_A
Cuts at: 815 869 815
Size: 54 3910
PmeI GTTT'AAAC
Cuts at: 961 961
Size: 3964
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3103 3135 3103
Size: 32 3932
PspOMI G'GGCC_C
Cuts at: 254 254
Size: 3964
PstI C_TGCA'G
Cuts at: 1009 1694 1009
Size: 685 3279
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3964
PvuII CAG'CTG
Cuts at: 251 362 682 948 1387 1747 251
Size: 111 320 266 439 360 2468
Fragments arranged by size:
2468 439 360 320 266 111
RsrII CG'GwC_CG
Cuts at: 2157 2157
Size: 3964
SacI G_AGCT'C
Cuts at: 976 976
Size: 3964
SacII CC_GC'GG
Cuts at: 438 741 438
Size: 303 3661
SalI G'TCGA_C
Cuts at: 999 999
Size: 3964
SapI GCTCTTCn'nnn_
Cuts at: 1985 2195 1985
Size: 210 3754
SbfI CC_TGCA'GG
Cuts at: 1009 1009
Size: 3964
ScaI AGT'ACT
Cuts at: 3481 3481
Size: 3964
SfcI C'TryA_G
Cuts at: 33 879 1005 1690 2685 2876 33
Size: 846 126 685 995 191 1121
Fragments arranged by size:
1121 995 846 685 191 126
SfoI GGC'GCC
Cuts at: 1394 1642 1394
Size: 248 3716
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3964
SmaI CCC'GGG
Cuts at: 664 984 664
Size: 320 3644
SmlI C'TyrA_G
Cuts at: 1117 2032 2526 2788 1117
Size: 915 494 262 2293
Fragments arranged by size:
2293 915 494 262
SnaBI TAC'GTA
Cuts at: 954 954
Size: 3964
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3964
SphI G_CATG'C
Cuts at: 1015 1515 1015
Size: 500 3464
StyI C'CwwG_G
Cuts at: 404 767 1096 1450 404
Size: 363 329 354 2918
Fragments arranged by size:
2918 363 354 329
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 218 805 1545 1678 2137 2348 218
Size: 587 740 133 459 211 1834
Fragments arranged by size:
1834 740 587 459 211 133
TatI w'GTAC_w
Cuts at: 746 3479 746
Size: 2733 1231
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 251 283 3481 3513 251
Size: 32 3198 32 702
Fragments arranged by size:
3198 702 32 32
Tth111I GACn'n_nGTC
Cuts at: 715 1759 715
Size: 1044 2920
XbaI T'CTAG_A
Cuts at: 993 993
Size: 3964
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 381 543 1457 381
Size: 162 914 2888
Fragments arranged by size:
2888 914 162
XmaI C'CCGG_G
Cuts at: 662 982 662
Size: 320 3644
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AloI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NruI NspI PciI PflMI PfoI PmeI PpiI
PspOMI PstI PvuI PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SnaBI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AscI
AvrII BaeI BbsI BbvCI BcgI BfrBI BmgBI BmtI
Bpu10I BsiWI BsmBI BssHII BstEII BstXI BstZ17I Bsu36I
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NsiI PacI PasI
PmlI PpuMI PshAI PsiI PspXI PsrI SanDI SexAI
SfiI SgrAI SrfI SspI StuI SwaI XhoI XmnI
ZraI