Restriction map for
pF1KSDF0400
(Circular) (Six-base) MAPSORT of: pF1KSDF0400.seq Check: 6768 from: 1 to: 5317
pF1KSDF0400 5317 bp
With 182 enzymes: SgfI *
June 20, 2008 10:36 ..
AccI GT'mk_AC
Cuts at: 2353 2353
Size: 5317
Acc65I G'GTAC_C
Cuts at: 2331 2331
Size: 5317
AclI AA'CG_TT
Cuts at: 1361 5157 1361
Size: 3796 1521
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1285 1690 1905 3140 3572 4321 4589 1285
Size: 405 215 1235 432 749 268 2013
Fragments arranged by size:
2013 1235 749 432 405 268 215
AflIII A'CryG_T
Cuts at: 2667 3773 4750 2667
Size: 1106 977 3234
Fragments arranged by size:
3234 1106 977
AgeI A'CCGG_T
Cuts at: 1487 3705 1487
Size: 2218 3099
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 907 907
Size: 5317
AlwNI CAG_nnn'CTG
Cuts at: 375 1140 2620 4189 375
Size: 765 1480 1569 1503
Fragments arranged by size:
1569 1503 1480 765
ApaI G_GGCC'C
Cuts at: 162 996 2107 2203 162
Size: 834 1111 96 3276
Fragments arranged by size:
3276 1111 834 96
ApaLI G'TGCA_C
Cuts at: 4087 4087
Size: 5317
ApoI r'AATT_y
Cuts at: 440 2319 4670 4926 440
Size: 1879 2351 256 831
Fragments arranged by size:
2351 1879 831 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5317
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5317
AvaI C'yCGr_G
Cuts at: 380 989 2088 2175 2335 380
Size: 609 1099 87 160 3362
Fragments arranged by size:
3362 1099 609 160 87
BamHI G'GATC_C
Cuts at: 2340 2340
Size: 5317
BanI G'GyrC_C
Cuts at: 168 350 843 2045 2254 2331 2745 2993 3028
Size: 182 493 1202 209 77 414 248 35
Cuts at: 3028 168
Size: 2457
Fragments arranged by size:
2457 1202 493 414 248 209 182 77
35
BanII G_rGCy'C
Cuts at: 162 290 395 996 1049 1874 1912 2107 2203
Size: 128 105 601 53 825 38 195 96
Cuts at: 2203 2329 3359 162
Size: 126 1030 2120
Fragments arranged by size:
2120 1030 825 601 195 128 126 105
96 53 38
BbeI G_GCGC'C
Cuts at: 354 847 2749 2997 354
Size: 493 1902 248 2674
Fragments arranged by size:
2674 1902 493 248
BbsI GAAGACnn'nnnn_
Cuts at: 823 855 1620 823
Size: 32 765 4520
Fragments arranged by size:
4520 765 32
BbvCI CC'TCA_GC
Cuts at: 745 745
Size: 5317
BciVI GTATCCnnnnn_n'
Cuts at: 3213 3976 5317 3213
Size: 763 1341 3213
Fragments arranged by size:
3213 1341 763
BclI T'GATC_A
Cuts at: 2835 2835
Size: 5317
BglI GCCn_nnn'nGGC
Cuts at: 431 1000 1473 431
Size: 569 473 4275
Fragments arranged by size:
4275 569 473
BglII A'GATC_T
Cuts at: 2830 4869 2830
Size: 2039 3278
BlpI GC'TnA_GC
Cuts at: 490 1945 2427 490
Size: 1455 482 3380
Fragments arranged by size:
3380 1455 482
Bme1580I G_kGCm'C
Cuts at: 162 586 996 1769 1896 2107 2203 2259 2940
Size: 424 410 773 127 211 96 56 681
Cuts at: 2940 3033 4091 162
Size: 93 1058 1388
Fragments arranged by size:
1388 1058 773 681 424 410 211 127
96 93 56
BmrI ACTGGGnnnn_n'
Cuts at: 2527 2608 2943 5089 2527
Size: 81 335 2146 2755
Fragments arranged by size:
2755 2146 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 729 2114 3682 729
Size: 1385 1568 2364
Fragments arranged by size:
2364 1568 1385
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 562 970 1117 1228 1662 1692 562
Size: 408 147 111 434 30 4187
Fragments arranged by size:
4187 434 408 147 111 30
Bpu10I CC'TnA_GC
Cuts at: 745 745
Size: 5317
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2491 3370 3864 4162 2491
Size: 879 494 298 3646
Fragments arranged by size:
3646 879 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 5021 5021
Size: 5317
BsaAI yAC'GTr
Cuts at: 2307 2668 2307
Size: 361 4956
BsaBI GATnn'nnATC
Cuts at: 6 2829 4868 6
Size: 2823 2039 455
Fragments arranged by size:
2823 2039 455
BsaHI Gr'CG_yC
Cuts at: 226 351 844 2746 2994 3698 5194 226
Size: 125 493 1902 248 704 1496 349
Fragments arranged by size:
1902 1496 704 493 349 248 125
BsaWI w'CCGG_w
Cuts at: 1487 2009 2504 3025 3705 3979 4126 4537 1487
Size: 522 495 521 680 274 147 411 2267
Fragments arranged by size:
2267 680 522 521 495 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 172 202 1105 1135 1390 1420 1894 1924 2047
Size: 30 903 30 255 30 474 30 123
Cuts at: 2047 2077 2104 2134 172
Size: 30 27 30 3355
Fragments arranged by size:
3355 903 474 255 123 30 30 30
30 30 30 27
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 196 733 757 858 967 1126 1414 1423 1540
Size: 537 24 101 109 159 288 9 117
Cuts at: 1540 1729 1788 1924 196
Size: 189 59 136 3589
Fragments arranged by size:
3589 537 288 189 159 136 117 109
101 59 24 9
BseYI C'CCAG_C
Cuts at: 825 1379 2287 2740 3498 4077 4573 825
Size: 554 908 453 758 579 496 1569
Fragments arranged by size:
1569 908 758 579 554 496 453
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 319 913 319
Size: 594 4723
BsiEI CG_ry'CG
Cuts at: 104 2197 2857 2903 4113 104
Size: 2093 660 46 1210 1308
Fragments arranged by size:
2093 1308 1210 660 46
BsiHKAI G_wGCw'C
Cuts at: 1739 2329 3107 3297 4091 1739
Size: 590 778 190 794 2965
Fragments arranged by size:
2965 794 778 590 190
BsmBI CGTCTCn'nnnn_
Cuts at: 1299 1974 1299
Size: 675 4642
Bsp1286I G_dGCh'C
Cuts at: 162 290 395 586 996 1049 1739 1769 1874
Size: 128 105 191 410 53 690 30 105
Cuts at: 1874 1896 1912 2107 2203 2259 2329 2940 3033
Size: 22 16 195 96 56 70 611 93
Cuts at: 3033 3107 3297 3359 4091 162
Size: 74 190 62 732 1388
Fragments arranged by size:
1388 732 690 611 410 195 191 190
128 105 105 96 93 74 70 62
56 53 30 22 16
BspEI T'CCGG_A
Cuts at: 2009 4537 2009
Size: 2528 2789
BspHI T'CATG_A
Cuts at: 4493 4493
Size: 5317
BspMI ACCTGCnnnn'nnnn_
Cuts at: 494 1031 1148 2019 2365 2579 2881 3262 494
Size: 537 117 871 346 214 302 381 2549
Fragments arranged by size:
2549 871 537 381 346 302 214 117
BsrBI CCG'CTC
Cuts at: 50 156 207 3607 3661 5147 5312 50
Size: 106 51 3400 54 1486 165 55
Fragments arranged by size:
3400 1486 165 106 55 54 51
BsrDI GCAATG_nn'
Cuts at: 3227 3227
Size: 5317
BsrFI r'CCGG_y
Cuts at: 329 1487 1499 3313 3494 3705 329
Size: 1158 12 1814 181 211 1941
Fragments arranged by size:
1941 1814 1158 211 181 12
BsrGI T'GTAC_A
Cuts at: 719 1406 719
Size: 687 4630
BssSI C'ACGA_G
Cuts at: 3586 3946 3586
Size: 360 4957
BstAPI GCAn_nnn'nTGC
Cuts at: 306 2692 306
Size: 2386 2931
BstBI TT'CG_AA
Cuts at: 110 110
Size: 5317
BstEII G'GTnAC_C
Cuts at: 1458 1575 1458
Size: 117 5200
BstXI CCAn_nnnn'nTGG
Cuts at: 586 586
Size: 5317
BstYI r'GATC_y
Cuts at: 1 2340 2807 2830 3165 3411 4425 4511 4523
Size: 2339 467 23 335 246 1014 86 12
Cuts at: 4523 4869 1
Size: 346 449
Fragments arranged by size:
2339 1014 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 136 290 1391 1439 2191 2219 136
Size: 154 1101 48 752 28 3234
Fragments arranged by size:
3234 1101 752 154 48 28
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1140 3265 3446 3609 1140
Size: 2125 181 163 2848
Fragments arranged by size:
2848 2125 181 163
BtsI GCAGTG_nn'
Cuts at: 1769 2558 1769
Size: 789 4528
DraI TTT'AAA
Cuts at: 2314 2314
Size: 5317
DraIII CAC_nnn'GTG
Cuts at: 2052 2052
Size: 5317
DrdI GACnn_nn'nnGTC
Cuts at: 3021 3881 3021
Size: 860 4457
EaeI y'GGCC_r
Cuts at: 1271 1884 2194 2900 3074 3465 3492 4839 1271
Size: 613 310 706 174 391 27 1347 1749
Fragments arranged by size:
1749 1347 706 613 391 310 174 27
EagI C'GGCC_G
Cuts at: 2194 2900 2194
Size: 706 4611
EarI CTCTTCn'nnn_
Cuts at: 433 682 1597 1639 1822 3338 3548 433
Size: 249 915 42 183 1516 210 2202
Fragments arranged by size:
2202 1516 915 249 210 183 42
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 529 718 807 2256 3523 3835 3981 5127 529
Size: 189 89 1449 1267 312 146 1146 719
Fragments arranged by size:
1449 1267 1146 719 312 189 146 89
EcoICRI GAG'CTC
Cuts at: 2327 2327
Size: 5317
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 562 970 1117 1228 1285 1662 1690 1692 1905
Size: 408 147 111 57 377 28 2 213
Cuts at: 1905 3140 3572 4321 4589 562
Size: 1235 432 749 268 1290
Fragments arranged by size:
1290 1235 749 432 408 377 268 213
147 111 57 28 2
EcoNI CCTnn'n_nnAGG
Cuts at: 358 551 2053 358
Size: 193 1502 3622
Fragments arranged by size:
3622 1502 193
EcoO109I rG'GnC_Cy
Cuts at: 159 384 423 556 656 1177 2058 2104 2187
Size: 225 39 133 100 521 881 46 83
Cuts at: 2187 2200 2454 159
Size: 13 254 3022
Fragments arranged by size:
3022 881 521 254 225 133 100 83
46 39 13
EcoRI G'AATT_C
Cuts at: 440 2319 440
Size: 1879 3438
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3334 3334
Size: 5317
FspI TGC'GCA
Cuts at: 3096 3096
Size: 5317
HaeII r_GCGC'y
Cuts at: 267 330 354 409 847 857 1073 2749 2997
Size: 63 24 55 438 10 216 1676 248
Cuts at: 2997 4021 5000 267
Size: 1024 979 584
Fragments arranged by size:
1676 1024 979 584 438 248 216 63
55 24 10
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1477 1509 2047 2079 3157 3187 3189 3219 1477
Size: 32 538 32 1078 30 2 30 3575
Fragments arranged by size:
3575 1078 538 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1032 1167 1236 2354 1032
Size: 135 69 1118 3995
Fragments arranged by size:
3995 1118 135 69
HindIII A'AGCT_T
Cuts at: 510 2126 510
Size: 1616 3701
KasI G'GCGC_C
Cuts at: 350 843 2745 2993 350
Size: 493 1902 248 2674
Fragments arranged by size:
2674 1902 493 248
KpnI G_GTAC'C
Cuts at: 2335 2335
Size: 5317
MluI A'CGCG_T
Cuts at: 4750 4750
Size: 5317
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3988 4172 3988
Size: 184 5133
MscI TGG'CCA
Cuts at: 1273 1886 3076 1273
Size: 613 1190 3514
Fragments arranged by size:
3514 1190 613
MslI CAynn'nnrTG
Cuts at: 889 1582 3304 3431 889
Size: 693 1722 127 2775
Fragments arranged by size:
2775 1722 693 127
MspA1I CmG'CkG
Cuts at: 279 292 318 372 753 972 1206 1340 2019
Size: 13 26 54 381 219 234 134 679
Cuts at: 2019 2193 2221 2291 2426 2740 3100 4115 4360
Size: 174 28 70 135 314 360 1015 245
Cuts at: 4360 279
Size: 1236
Fragments arranged by size:
1236 1015 679 381 360 314 245 234
219 174 135 134 70 54 28 26
13
NaeI GCC'GGC
Cuts at: 331 3496 331
Size: 3165 2152
NarI GG'CG_CC
Cuts at: 351 844 2746 2994 351
Size: 493 1902 248 2674
Fragments arranged by size:
2674 1902 493 248
NgoMIV G'CCGG_C
Cuts at: 329 3494 329
Size: 3165 2152
NruI TCG'CGA
Cuts at: 2067 3678 2067
Size: 1611 3706
NspI r_CATG'y
Cuts at: 643 2368 2868 3777 643
Size: 1725 500 909 2183
Fragments arranged by size:
2183 1725 909 500
PasI CC'CwG_GG
Cuts at: 163 1147 1299 1770 163
Size: 984 152 471 3710
Fragments arranged by size:
3710 984 471 152
PciI A'CATG_T
Cuts at: 3773 3773
Size: 5317
PflMI CCAn_nnn'nTGG
Cuts at: 1079 1905 2167 1079
Size: 826 262 4229
Fragments arranged by size:
4229 826 262
PfoI T'CCnGG_A
Cuts at: 1850 1850
Size: 5317
PmeI GTTT'AAAC
Cuts at: 2314 2314
Size: 5317
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4456 4488 4456
Size: 32 5285
PpuMI rG'GwC_Cy
Cuts at: 1177 2058 1177
Size: 881 4436
PspOMI G'GGCC_C
Cuts at: 158 992 2103 2199 158
Size: 834 1111 96 3276
Fragments arranged by size:
3276 1111 834 96
PstI C_TGCA'G
Cuts at: 1764 2362 3047 1764
Size: 598 685 4034
Fragments arranged by size:
4034 685 598
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5317
PvuII CAG'CTG
Cuts at: 279 972 1206 1340 2019 2740 3100 279
Size: 693 234 134 679 721 360 2496
Fragments arranged by size:
2496 721 693 679 360 234 134
RsrII CG'GwC_CG
Cuts at: 3510 3510
Size: 5317
SacI G_AGCT'C
Cuts at: 2329 2329
Size: 5317
SacII CC_GC'GG
Cuts at: 293 2194 2222 293
Size: 1901 28 3388
Fragments arranged by size:
3388 1901 28
SalI G'TCGA_C
Cuts at: 2352 2352
Size: 5317
SanDI GG'GwC_CC
Cuts at: 1177 1177
Size: 5317
SapI GCTCTTCn'nnn_
Cuts at: 3338 3548 3338
Size: 210 5107
SbfI CC_TGCA'GG
Cuts at: 2362 2362
Size: 5317
ScaI AGT'ACT
Cuts at: 1093 4834 1093
Size: 3741 1576
SexAI A'CCwGG_T
Cuts at: 2048 2048
Size: 5317
SfcI C'TryA_G
Cuts at: 33 1760 2358 3043 4038 4229 33
Size: 1727 598 685 995 191 1121
Fragments arranged by size:
1727 1121 995 685 598 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 1000 1000
Size: 5317
SfoI GGC'GCC
Cuts at: 352 845 2747 2995 352
Size: 493 1902 248 2674
Fragments arranged by size:
2674 1902 493 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5317
SgrAI Cr'CCGG_yG
Cuts at: 329 329
Size: 5317
SmaI CCC'GGG
Cuts at: 382 991 2177 2337 382
Size: 609 1186 160 3362
Fragments arranged by size:
3362 1186 609 160
SmlI C'TyrA_G
Cuts at: 2470 3385 3879 4141 2470
Size: 915 494 262 3646
Fragments arranged by size:
3646 915 494 262
SnaBI TAC'GTA
Cuts at: 2307 2307
Size: 5317
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5317
SphI G_CATG'C
Cuts at: 2368 2868 2368
Size: 500 4817
StyI C'CwwG_G
Cuts at: 387 857 1866 2449 2803 387
Size: 470 1009 583 354 2901
Fragments arranged by size:
2901 1009 583 470 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 434 2898 3031 3490 3701 434
Size: 2464 133 459 211 2050
Fragments arranged by size:
2464 2050 459 211 133
TatI w'GTAC_w
Cuts at: 719 1091 1406 1658 4832 719
Size: 372 315 252 3174 1204
Fragments arranged by size:
3174 1204 372 315 252
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 172 204 4834 4866 172
Size: 32 4630 32 623
Fragments arranged by size:
4630 623 32 32
Tth111I GACn'n_nGTC
Cuts at: 3112 3112
Size: 5317
XbaI T'CTAG_A
Cuts at: 2346 2346
Size: 5317
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 604 1548 2810 604
Size: 944 1262 3111
Fragments arranged by size:
3111 1262 944
XmaI C'CCGG_G
Cuts at: 380 989 2175 2335 380
Size: 609 1186 160 3362
Fragments arranged by size:
3362 1186 609 160
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NruI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PspOMI PstI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI AscI
AvrII BaeI BcgI BfrBI BmgBI BmtI BsiWI BsmI
BssHII BstZ17I Bsu36I ClaI CspCI EcoRV FseI FspAI
HpaI MfeI NcoI NdeI NheI NotI NsiI PacI
PmlI PshAI PsiI PspXI PsrI SrfI SspI StuI
SwaI XhoI XmnI ZraI