Restriction map for
pF1KB9867
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# NO CUT ENZYMES ( 135 enzymes) #
##################################
Acc65I AccI AccIII AclI AcsI
AflII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AvaI AviII AvrII BamHI BanI
BbeI BbrPI BcgI BcnI BfaI
BfrI BglI BlnI BsaAI BsaBI
BseAI BsiEI BsiWI BsmI BsoBI
Bsp1407I BspDI BspEI BspLU11I BsrBI
BsrFI BsrGI BssHII Bst1107I BstAPI
BstBI BstZ17I Bsu36I Cfr10I ClaI
CpoI DraI DraIII EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoRI EcoRV EcoT22I FauI FseI
FspI HaeII HincII HindII HpaI
KasI KpnI Ksp632I KspI MaeI
MamI MfeI MluI MroI MunI
NaeI NarI NciI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Van91I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AatII 1285 GACGTC GACGT^C
AccII 1104 CGCG CG^CG
AcyI 1285 GACGTC G[GA]^CG[CT]C
AfaI 454 GTAC GT^AC
AflIII 941 ACGTGT A^C[GA][CT]GT
AlwNI 245 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 315 GTGCTC G[AT]GC[AT]^C
BalI 620 TGGCCA TGG^CCA
BanII 1185 GGGCTC G[GA]GC[CT]^C
BbsI 813 GAAGAC GAAGAC(2/6)
BbvCI 567 CCTCAGC CCTCAGC(-5/-2)
BciVI 68 GTATCC GTATCC(6/5)
BglII 207 AGATCT A^GATCT
BlpI 480 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 480 GCTCAGC GC^T[ACGT]AGC
BpuAI 813 GAAGAC GAAGAC(2/6)
BsaHI 1285 GACGTC G[GA]^CG[CT]C
BsaI 541 GAGACC GGTCTC(1/5)
BsgI 475 GTGCAG GTGCAG(16/14)
BsiHKAI 315 GTGCTC G[AT]GC[AT]^C
BspMI 158 ACCTGC ACCTGC(4/8)
BsrDI 681 CATTGC GCAATG(2/0)
BssSI 1130 CACGAG CACGAG(-5/-1)
BstEII 171 GGTGACC G^GT[ACGT]ACC
BstPI 171 GGTGACC G^GT[ACGT]ACC
BstUI 1104 CGCG CG^CG
CelII 480 GCTCAGC GC^T[ACGT]AGC
CfoI 678 GCGC GCG^C
DrdI 1279 GACACTGACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 23 CCATGG C^C[GA][CT]GG
EcoO65I 171 GGTGACC G^GT[ACGT]ACC
HgaI 906 GACGC GACGC(5/10)
HhaI 678 GCGC GCG^C
Hin1I 1285 GACGTC G[GA]^CG[CT]C
HinP1I 678 GCGC G^CGC
HindIII 603 AAGCTT A^AGCTT
MluNI 620 TGGCCA TGG^CCA
MscI 620 TGGCCA TGG^CCA
MvnI 1104 CGCG CG^CG
NcoI 23 CCATGG C^CATGG
NdeI 578 CATATG CA^TATG
NspI 1176 ACATGC [GA]CATG^[CT]
RsaI 454 GTAC GT^AC
TaqI 752 TCGA T^CGA
TfiI 925 GATTC G^A[AT]TC
TthHB8I 752 TCGA T^CGA
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AspI 444 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
816 GACTCTGTC
BmyI 315 GTGCTC G[AGT]GC[ACT]^C
1185 GGGCTC
BsaWI 386 TCCGGT [AT]^CCGG[AT]
765 ACCGGA
BseRI 1188 CTCCTC GAGGAG(10/8)
1210 GAGGAG
Bsp1286I 315 GTGCTC G[AGT]GC[ACT]^C
1185 GGGCTC
BspHI 626 TCATGA T^CATGA
1122 TCATGA
BsrI 34 ACTGG ACTGG(1/-1)
472 CCAGT
DraII 277 GGGTCCT [GA]G^G[ACGT]CC[CT]
1196 AGGACCT
EaeI 218 TGGCCG [CT]^GGCC[GA]
620 TGGCCA
EcoO109I 277 GGGTCCT [GA]G^G[ACGT]CC[CT]
1196 AGGACCT
HapII 387 CCGG C^CGG
766 CCGG
HpaII 387 CCGG C^CGG
766 CCGG
MaeII 941 ACGT A^CGT
1286 ACGT
MseI 779 TTAA T^TAA
998 TTAA
MspA1I 483 CAGCTG C[AC]G^C[GT]G
1217 CAGCTG
MspI 387 CCGG C^CGG
766 CCGG
PflFI 444 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
816 GACTCTGTC
PpuMI 277 GGGTCCT [GA]G^G[AT]CC[CT]
1196 AGGACCT
PvuII 483 CAGCTG CAG^CTG
1217 CAGCTG
RcaI 626 TCATGA T^CATGA
1122 TCATGA
SfcI 651 CTACAG C^T[GA][CT]AG
1041 CTACAG
Tru9I 779 TTAA T^TAA
998 TTAA
Tsp509I 718 AATT ^AATT
967 AATT
Tth111I 444 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
816 GACTCTGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 53 enzymes) #
#########################################
AciI AluI AlwI AvaII BbvI
BclI BpmI BsaJI BsiYI BslI
BsmAI BsmBI BsmFI BssKI BstF5I
BstNI BstXI BstYI Cac8I Cfr13I
DdeI DpnII EcoNI EcoRII EcoT14I
FbaI Fnu4HI FokI HaeIII HinfI
HphI ItaI MaeIII MboI MboII
MflI MnlI MslI MvaI MwoI
NdeII NlaIII NlaIV PleI Sau3AI
Sau96I ScrFI SfaNI StyI TseI
Tsp45I TspRI XhoII