Restriction map for
pF1KA0082
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# NO CUT ENZYMES ( 92 enzymes) #
##################################
Acc65I AclI AflII AflIII AgeI
Alw44I AocI Aor51HI ApaLI AscI
AseI AsnI Asp718I AspI AvrII
BbeI BbrPI BcgI BciVI BfrI
BlnI BsaAI BsgI BsiEI BsiWI
BspDI BspHI BspLU11I BsrBI BssHII
Bst1107I BstEII BstPI BstXI BstZ17I
Bsu36I ClaI CpoI DraI DrdI
EagI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I FseI HpaI KasI
KpnI MaeIII MfeI MluI MunI
NarI NdeI NheI NotI NruI
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SalI ScaI SexAI SfiI SfoI
SgrAI SmlI SnaBI SpeI StuI
SwaI Tsp45I Tth111I Van91I XbaI
XcmI XhoI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AccI 1249 GTCTAC GT^[AC][GT]AC
AccII 761 CGCG CG^CG
AccIII 1184 TCCGGA T^CCGGA
AhdI 2455 GACAAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 409 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 909 GGGCCC GGGCC^C
AspEI 2455 GACAAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 647 GAGCTC G[AT]GC[AT]^C
AviII 829 TGCGCA TGC^GCA
BalI 284 TGGCCA TGG^CCA
BamHI 1619 GGATCC G^GATCC
BclI 1454 TGATCA T^GATCA
BglII 1829 AGATCT A^GATCT
BsaWI 1184 TCCGGA [AT]^CCGG[AT]
BseAI 1184 TCCGGA T^CCGGA
BsiHKAI 647 GAGCTC G[AT]GC[AT]^C
BsmBI 267 CGTCTC CGTCTC(1/5)
Bsp1407I 2117 TGTACA T^GTACA
BspEI 1184 TCCGGA T^CCGGA
BsrGI 2117 TGTACA T^GTACA
BssSI 1634 CTCGTG CACGAG(-5/-1)
BstAPI 61 GCAGAGCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 961 TTCGAA TT^CGAA
BstUI 761 CGCG CG^CG
Eam1105I 2455 GACAAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 2036 GAATTC G^AATTC
EcoRV 1003 GATATC GAT^ATC
EcoT22I 206 ATGCAT ATGCA^T
FbaI 1454 TGATCA T^GATCA
FspI 829 TGCGCA TGC^GCA
HaeII 1170 GGCGCT [GA]GCGC^[CT]
HgaI 266 GCGTC GACGC(5/10)
HincII 1429 GTCAAC GT[CT]^[GA]AC
HindII 1429 GTCAAC GT[CT]^[GA]AC
KspI 760 CCGCGG CCGC^GG
MluNI 284 TGGCCA TGG^CCA
MroI 1184 TCCGGA T^CCGGA
MscI 284 TGGCCA TGG^CCA
MvnI 761 CGCG CG^CG
NaeI 2390 GCCGGC GCC^GGC
NcoI 2234 CCATGG C^CATGG
NgoMIV 2390 GCCGGC G^CCGGC
NsiI 206 ATGCAT ATGCA^T
NspV 961 TTCGAA TT^CGAA
SacI 647 GAGCTC GAGCT^C
SacII 760 CCGCGG CCGC^GG
SbfI 1122 CCTGCAGG CCTGCA^GG
SfuI 961 TTCGAA TT^CGAA
SmaI 1895 CCCGGG CCC^GGG
SphI 879 GCATGC GCATG^C
Sse8387I 1122 CCTGCAGG CCTGCA^GG
SspBI 2117 TGTACA T^GTACA
SspI 1390 AATATT AAT^ATT
XmaCI 1895 CCCGGG C^CCGGG
XmaI 1895 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AatII 1426 GACGTC GACGT^C
2473 GACGTC
AcyI 1426 GACGTC G[GA]^CG[CT]C
2473 GACGTC
AvaI 656 CTCGGG C^[CT]CG[GA]G
1895 CCCGGG
BanI 2002 GGCACC G^G[CT][GA]CC
2185 GGCACC
BbsI 1881 GAAGAC GAAGAC(2/6)
688 GTCTTC
BbvCI 78 CCTCAGC CCTCAGC(-5/-2)
1134 CCTCAGC
BfaI 1873 CTAG C^TAG
2078 CTAG
BglI 836 GCCCAGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2076 GCCTAGTCGGC
BlpI 802 GCTGAGC GC^T[ACGT]AGC
2196 GCTCAGC
Bpu1102I 802 GCTGAGC GC^T[ACGT]AGC
2196 GCTCAGC
BpuAI 1881 GAAGAC GAAGAC(2/6)
688 GTCTTC
BsaBI 1698 GATTGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2160 GATGAAGATC
BsaHI 1426 GACGTC G[GA]^CG[CT]C
2473 GACGTC
BsaI 166 GAGACC GGTCTC(1/5)
1463 GAGACC
BsoBI 656 CTCGGG C^[CT]CG[GA]G
1895 CCCGGG
BsrDI 2361 CATTGC GCAATG(2/0)
709 GCAATG
BsrFI 2219 ACCGGC [GA]^CCGG[CT]
2390 GCCGGC
CelII 802 GCTGAGC GC^T[ACGT]AGC
2196 GCTCAGC
CfoI 830 GCGC GCG^C
1171 GCGC
Cfr10I 2219 ACCGGC [GA]^CCGG[CT]
2390 GCCGGC
DraIII 589 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
1565 CACTGAGTG
DsaI 760 CCGCGG C^C[GA][CT]GG
2234 CCATGG
FauI 1010 CCCGC CCCGC(4/6)
762 GCGGG
HhaI 830 GCGC GCG^C
1171 GCGC
Hin1I 1426 GACGTC G[GA]^CG[CT]C
2473 GACGTC
HinP1I 830 GCGC G^CGC
1171 GCGC
HindIII 277 AAGCTT A^AGCTT
2292 AAGCTT
MaeI 1873 CTAG C^TAG
2078 CTAG
MamI 1698 GATTGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2160 GATGAAGATC
MseI 1394 TTAA T^TAA
2027 TTAA
NspI 746 GCATGT [GA]CATG^[CT]
879 GCATGC
PvuII 1144 CAGCTG CAG^CTG
1405 CAGCTG
SapI 577 GAAGAGC GCTCTTC(1/4)
2394 GCTCTTC
Tru9I 1394 TTAA T^TAA
2027 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 77 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI Asp700I AvaII BanII BbvI
BcnI BmyI BpmI BsaJI BseRI
BsiYI BslI BsmAI BsmFI BsmI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII DraII EaeI EarI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
Ksp632I MaeII MboI MboII MflI
MnlI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PleI PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TaqI
TfiI TseI Tsp509I TspRI TthHB8I
XhoII XmnI