Restriction map for
pF1KA0089
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# NO CUT ENZYMES ( 144 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AflIII AgeI AhdI Alw44I AocI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AviII
AvrII BalI BamHI BbrPI BbvCI
BcgI BciVI BcnI BglI BlnI
BlpI BpmI Bpu1102I BsaAI BsaBI
BsaWI BseAI BseRI BsiHKAI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstEII
BstPI BstUI BstZ17I Bsu36I Cac8I
CelII Cfr10I ClaI CpoI DraI
DraIII DrdI DsaI Eam1105I Eco81I
EcoO65I EcoRI EcoRV EcoT22I FseI
FspI HapII HgaI HincII HindII
HindIII HpaI HpaII KpnI KspI
MamI MfeI MluI MluNI MroI
MscI MspI MunI MvnI NaeI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI Tsp45I Tth111I Van91I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AccI 747 GTCTAC GT^[AC][GT]AC
AcyI 9 GGCGCC G[GA]^CG[CT]C
AfaI 924 GTAC GT^AC
AflII 613 CTTAAG C^TTAAG
AlwI 725 GGATC GGATC(4/5)
Aor51HI 342 AGCGCT AGC^GCT
ApaI 378 GGGCCC GGGCC^C
AvaII 901 GGACC G^G[AT]CC
BanI 9 GGCGCC G^G[CT][GA]CC
BbeI 9 GGCGCC GGCGC^C
BclI 788 TGATCA T^GATCA
BfaI 763 CTAG C^TAG
BfrI 613 CTTAAG C^TTAAG
BglII 986 AGATCT A^GATCT
BsaHI 9 GGCGCC G[GA]^CG[CT]C
BsaI 493 GAGACC GGTCTC(1/5)
BsgI 253 GTGCAG GTGCAG(16/14)
BsiEI 680 CGGCCG CG[GA][CT]^CG
BspMI 266 ACCTGC ACCTGC(4/8)
BsrDI 459 CATTGC GCAATG(2/0)
BstBI 563 TTCGAA TT^CGAA
BstXI 464 CCAATGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DdeI 241 CTTAG C^T[ACGT]AG
EagI 680 CGGCCG C^GGCCG
EclXI 680 CGGCCG C^GGCCG
Eco47III 342 AGCGCT AGC^GCT
Eco52I 680 CGGCCG C^GGCCG
EcoNI 357 CCTCATCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 897 CCAAGG C^C[AT][AT]GG
FauI 773 GCGGG CCCGC(4/6)
FbaI 788 TGATCA T^GATCA
Hin1I 9 GGCGCC G[GA]^CG[CT]C
KasI 9 GGCGCC G^GCGCC
MaeI 763 CTAG C^TAG
MaeII 80 ACGT A^CGT
MaeIII 800 GTTAC ^GT[ACGT]AC
MseI 614 TTAA T^TAA
NarI 9 GGCGCC GG^CGCC
NspV 563 TTCGAA TT^CGAA
PvuII 53 CAGCTG CAG^CTG
RsaI 924 GTAC GT^AC
SfoI 9 GGCGCC GGC^GCC
SfuI 563 TTCGAA TT^CGAA
SmlI 613 CTTAAG C^T[CT][GA]AG
StuI 830 AGGCCT AGG^CCT
StyI 897 CCAAGG C^C[AT][AT]GG
TaqI 564 TCGA T^CGA
Tru9I 614 TTAA T^TAA
TthHB8I 564 TCGA T^CGA
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AcsI 97 AAATTT [GA]^AATT[CT]
558 AAATTT
AlwNI 303 CAGAATCTG CAG[ACGT][ACGT][ACGT]^CTG
587 CAGACACTG
ApoI 97 AAATTT [GA]^AATT[CT]
558 AAATTT
AvaI 36 CTCGGG C^[CT]CG[GA]G
382 CCCGAG
BanII 33 GGGCTC G[GA]GC[CT]^C
378 GGGCCC
BbsI 849 GAAGAC GAAGAC(2/6)
1028 GTCTTC
BpuAI 849 GAAGAC GAAGAC(2/6)
1028 GTCTTC
BsmAI 493 GAGAC GTCTC(1/5)
665 GAGAC
BsmFI 696 GGGAC GGGAC(10/14)
945 GGGAC
BsoBI 36 CTCGGG C^[CT]CG[GA]G
382 CCCGAG
BstYI 725 GGATCT [GA]^GATC[CT]
986 AGATCT
DraII 377 AGGGCCC [GA]G^G[ACGT]CC[CT]
378 GGGCCCC
EarI 532 CTCTTC CTCTTC(1/4)
859 GAAGAG
EcoO109I 377 AGGGCCC [GA]G^G[ACGT]CC[CT]
378 GGGCCCC
HaeII 9 GGCGCC [GA]GCGC^[CT]
342 AGCGCT
HphI 353 TCACC GGTGA(8/7)
791 TCACC
Ksp632I 532 CTCTTC CTCTTC(1/4)
859 GAAGAG
MflI 725 GGATCT [GA]^GATC[CT]
986 AGATCT
PleI 552 GACTC GAGTC(4/5)
698 GACTC
TfiI 305 GAATC G^A[AT]TC
350 GAATC
XcmI 335 CCAAGAAAGCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
854 CCATTGAAGAGTTGG
XhoII 725 GGATCT [GA]^GATC[CT]
986 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 43 enzymes) #
#########################################
AciI AluI BbvI BmyI BsaJI
BsiYI BslI Bsp1286I BsrI BssKI
BstF5I BstNI CfoI Cfr13I DpnII
EaeI EcoRII Fnu4HI FokI HaeIII
HhaI HinP1I HinfI ItaI MboI
MboII MnlI MslI MspA1I MvaI
MwoI NdeII NlaIII NlaIV PstI
Sau3AI Sau96I ScrFI SfaNI SfcI
TseI Tsp509I TspRI