Restriction map for
pF1KA0095
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# NO CUT ENZYMES ( 93 enzymes) #
##################################
Acc65I AccI AccIII AclI AflII
AgeI AhdI Alw44I AlwNI ApaLI
AscI AseI AsnI Asp718I AspEI
AspI AvrII BalI BamHI BbeI
BbrPI BfrI BglI BglII BlnI
BsaAI BsaBI BsaWI BseAI BsiEI
BsiWI BspDI BspEI BssHII Bst1107I
BstZ17I ClaI CpoI DraIII EaeI
EagI Eam1105I EclXI Eco52I EcoT22I
FseI HindIII HpaI KasI KpnI
KspI MamI MluI MluNI MroI
MscI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RsrII SacII SalI SapI SbfI
SfiI SfoI SgrAI SmaI SnaBI
Sse8387I SspI SwaI Tth111I XbaI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
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AatII 1192 GACGTC GACGT^C
Aor51HI 2144 AGCGCT AGC^GCT
ApaI 1130 GGGCCC GGGCC^C
Asp700I 2239 GAAGTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 749 TGCGCA TGC^GCA
BcgI 2426 CGAGGCTGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 3 GGATAC GTATCC(6/5)
BclI 2084 TGATCA T^GATCA
BsmBI 160 GAGACG CGTCTC(1/5)
BsmI 1899 GAATGC GAATGC(1/-1)
Bsp1407I 1601 TGTACA T^GTACA
BspHI 2450 TCATGA T^CATGA
BsrBI 1447 GAGCGG CCGCTC(-3/-3)
BsrDI 2007 CATTGC GCAATG(2/0)
BsrFI 708 ACCGGC [GA]^CCGG[CT]
BsrGI 1601 TGTACA T^GTACA
BssSI 1543 CACGAG CACGAG(-5/-1)
BstBI 878 TTCGAA TT^CGAA
BstEII 1552 GGTGACC G^GT[ACGT]ACC
BstPI 1552 GGTGACC G^GT[ACGT]ACC
BstXI 1542 CCACGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 708 ACCGGC [GA]^CCGG[CT]
DrdI 512 GACCCCCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 2144 AGCGCT AGC^GCT
EcoNI 2163 CCTGAATCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1552 GGTGACC G^GT[ACGT]ACC
EcoRI 428 GAATTC G^AATTC
EcoRV 2134 GATATC GAT^ATC
FbaI 2084 TGATCA T^GATCA
FspI 749 TGCGCA TGC^GCA
HincII 702 GTTGAC GT[CT]^[GA]AC
HindII 702 GTTGAC GT[CT]^[GA]AC
MfeI 871 CAATTG C^AATTG
MunI 871 CAATTG C^AATTG
NcoI 674 CCATGG C^CATGG
NspV 878 TTCGAA TT^CGAA
PflMI 225 CCAGCGATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 601 CTGCAG CTGCA^G
RcaI 2450 TCATGA T^CATGA
SacI 22 GAGCTC GAGCT^C
ScaI 1646 AGTACT AGT^ACT
SfuI 878 TTCGAA TT^CGAA
SpeI 1804 ACTAGT A^CTAGT
SphI 974 GCATGC GCATG^C
SspBI 1601 TGTACA T^GTACA
StuI 770 AGGCCT AGG^CCT
Van91I 225 CCAGCGATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 2420 CCAATGCGAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 2239 GAAGTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AccII 557 CGCG CG^CG
2341 CGCG
AlwI 105 GATCC GGATC(4/5)
1150 GATCC
AocI 1134 CCTCAGG CC^T[ACGT]AGG
1659 CCTCAGG
AspHI 22 GAGCTC G[AT]GC[AT]^C
1526 GTGCTC
AvaI 190 CTCGGG C^[CT]CG[GA]G
197 CTCGGG
BbsI 457 GAAGAC GAAGAC(2/6)
1336 GAAGAC
BbvCI 1536 CCTCAGC CCTCAGC(-5/-2)
373 GCTGAGG
BcnI 350 CCCGG CC^[GC]GG
1607 CCCGG
BfaI 1759 CTAG C^TAG
1805 CTAG
BlpI 1528 GCTCAGC GC^T[ACGT]AGC
1935 GCTGAGC
Bpu1102I 1528 GCTCAGC GC^T[ACGT]AGC
1935 GCTGAGC
BpuAI 457 GAAGAC GAAGAC(2/6)
1336 GAAGAC
BsaI 986 GAGACC GGTCTC(1/5)
194 GGTCTC
BsiHKAI 22 GAGCTC G[AT]GC[AT]^C
1526 GTGCTC
BsoBI 190 CTCGGG C^[CT]CG[GA]G
197 CTCGGG
BspLU11I 665 ACATGT A^CATGT
1691 ACATGT
BspMI 599 ACCTGC ACCTGC(4/8)
989 ACCTGC
BstUI 557 CGCG CG^CG
2341 CGCG
BstYI 104 AGATCC [GA]^GATC[CT]
1149 AGATCC
Bsu36I 1134 CCTCAGG CC^T[ACGT]AGG
1659 CCTCAGG
CelII 1528 GCTCAGC GC^T[ACGT]AGC
1935 GCTGAGC
DraI 1042 TTTAAA TTT^AAA
1506 TTTAAA
DsaI 674 CCATGG C^C[GA][CT]GG
1622 CCACGG
Eco81I 1134 CCTCAGG CC^T[ACGT]AGG
1659 CCTCAGG
HgaI 460 GACGC GACGC(5/10)
178 GCGTC
MaeI 1759 CTAG C^TAG
1805 CTAG
MflI 104 AGATCC [GA]^GATC[CT]
1149 AGATCC
MslI 540 CATCGAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
807 CACCCTTGTG
MspA1I 844 CAGCTG C[AC]G^C[GT]G
1030 CAGCTG
MvnI 557 CGCG CG^CG
2341 CGCG
NciI 350 CCCGG CC^[GC]GG
1607 CCCGG
PvuII 844 CAGCTG CAG^CTG
1030 CAGCTG
SexAI 611 ACCTGGT A^CC[AT]GGT
1048 ACCTGGT
SfcI 601 CTGCAG C^T[GA][CT]AG
922 CTACAG
TfiI 1746 GATTC G^A[AT]TC
2166 GAATC
XhoII 104 AGATCC [GA]^GATC[CT]
1149 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 80 enzymes) #
#########################################
AciI AcsI AcyI AfaI AflIII
AluI ApoI AvaII BanI BanII
BbvI BmyI BpmI BsaHI BsaJI
BseRI BsgI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstAPI
BstF5I BstNI Cac8I CfoI Cfr13I
DdeI DpnII DraII EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeII MaeIII MboI MboII
MnlI MseI MspI MvaI MwoI
NdeII NlaIII NlaIV NspI PleI
PpuMI RsaI Sau3AI Sau96I ScrFI
SfaNI SmlI StyI TaqI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I