Restriction map for
pF1KA0100
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# NO CUT ENZYMES ( 60 enzymes) #
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AclI AgeI AhdI Alw44I ApaLI
AscI AseI AsnI AspEI AviII
AvrII BbrPI BglI BlnI BsaAI
BsiEI BsiWI Bsp1407I BspDI BsrGI
BssHII Bst1107I BstZ17I ClaI CpoI
DraI EagI Eam1105I EclXI Eco52I
FseI FspI KspI MfeI MluI
MunI NdeI NotI NruI PacI
PaeR7I PinAI PmaCI PmeI PmlI
PpuMI PshBI Psp1406I PvuI RsrII
SacII SfiI SgrAI SmaI SnaBI
SspBI SwaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AatII 1821 GACGTC GACGT^C
Acc65I 3092 GGTACC G^GTACC
AccI 3128 GTCGAC GT^[AC][GT]AC
AccIII 2435 TCCGGA T^CCGGA
AflII 169 CTTAAG C^TTAAG
Asp718I 3092 GGTACC G^GTACC
AvaI 4067 CTCGGG C^[CT]CG[GA]G
BcgI 4275 GCATAGCAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 169 CTTAAG C^TTAAG
BsaI 2066 GAGACC GGTCTC(1/5)
BseAI 2435 TCCGGA T^CCGGA
BsoBI 4067 CTCGGG C^[CT]CG[GA]G
BspEI 2435 TCCGGA T^CCGGA
BsrBI 1965 CCGCTC CCGCTC(-3/-3)
BstAPI 1046 GCATTGTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 956 TTCGAA TT^CGAA
DrdI 1723 GACTTGAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 3559 GATATC GAT^ATC
EcoT22I 1465 ATGCAT ATGCA^T
HpaI 4546 GTTAAC GTT^AAC
KpnI 3092 GGTACC GGTAC^C
MroI 2435 TCCGGA T^CCGGA
NaeI 2279 GCCGGC GCC^GGC
NgoMIV 2279 GCCGGC G^CCGGC
NsiI 1465 ATGCAT ATGCA^T
NspV 956 TTCGAA TT^CGAA
SalI 3128 GTCGAC G^TCGAC
SapI 3909 GCTCTTC GCTCTTC(1/4)
SbfI 2295 CCTGCAGG CCTGCA^GG
SfuI 956 TTCGAA TT^CGAA
SpeI 4522 ACTAGT A^CTAGT
SphI 4892 GCATGC GCATG^C
Sse8387I 2295 CCTGCAGG CCTGCA^GG
SspI 3267 AATATT AAT^ATT
StuI 3889 AGGCCT AGG^CCT
XbaI 2511 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 23 enzymes) #
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AccII 18 CGCG CG^CG
5114 CGCG
AflIII 2024 ACATGT A^C[GA][CT]GT
4601 ACATGT
Aor51HI 334 AGCGCT AGC^GCT
1854 AGCGCT
AspI 829 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
5320 GACATTGTC
BciVI 444 GGATAC GTATCC(6/5)
5250 GGATAC
BclI 3666 TGATCA T^GATCA
4206 TGATCA
BsaBI 937 GATGAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4315 GATGGGGATC
BsmBI 2854 CGTCTC CGTCTC(1/5)
1819 GAGACG
BsmI 3038 GAATGC GAATGC(1/-1)
4200 GAATGC
BspLU11I 2024 ACATGT A^CATGT
4601 ACATGT
BstUI 18 CGCG CG^CG
5114 CGCG
Eco47III 334 AGCGCT AGC^GCT
1854 AGCGCT
EcoRI 1150 GAATTC G^AATTC
3821 GAATTC
FbaI 3666 TGATCA T^GATCA
4206 TGATCA
HgaI 1388 GCGTC GACGC(5/10)
3174 GCGTC
HindIII 960 AAGCTT A^AGCTT
5569 AAGCTT
MamI 937 GATGAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4315 GATGGGGATC
MvnI 18 CGCG CG^CG
5114 CGCG
NheI 1173 GCTAGC G^CTAGC
5952 GCTAGC
PflFI 829 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
5320 GACATTGTC
ScaI 4898 AGTACT AGT^ACT
5913 AGTACT
Tth111I 829 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
5320 GACATTGTC
XcmI 3370 CCACCTTTGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
5600 CCAACCTGCAGCTGG
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# 3 OR MORE CUT ENZYMES ( 139 enzymes) #
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AciI AcsI AcyI AfaI AluI
AlwI AlwNI AocI ApaI ApoI
Asp700I AspHI AvaII BalI BamHI
BanI BanII BbeI BbsI BbvCI
BbvI BcnI BfaI BglII BlpI
BmyI BpmI Bpu1102I BpuAI BsaHI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspHI BspMI BsrDI BsrFI BsrI
BssKI BssSI BstEII BstF5I BstNI
BstPI BstXI BstYI Bsu36I Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DraIII DsaI EaeI
EarI Eco81I EcoNI EcoO109I EcoO65I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI KasI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MwoI NarI
NciI NcoI NdeII NlaIII NlaIV
NspI PflMI PleI PshAI PstI
PvuII RcaI RsaI SacI Sau3AI
Sau96I ScrFI SexAI SfaNI SfcI
SfoI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XhoII XmnI