Restriction map for
pF1KA0128
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# NO CUT ENZYMES ( 140 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AfaI AflII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspI AvaI AviII BalI BamHI
BanI BbeI BbrPI BbsI BbvCI
BcgI BclI BfrI BglI BpuAI
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BsiWI BsmBI BsoBI Bsp1407I
BspEI BspHI BsrBI BsrFI BsrGI
BssHII Bst1107I BstAPI BstEII BstPI
BstZ17I Bsu36I Cfr10I CpoI DraI
DraII DraIII DrdI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRV EcoT22I
FbaI FseI FspI HaeII HgaI
Hin1I HindIII HpaI KasI KpnI
KspI MamI MfeI MluI MluNI
MroI MscI MseI MunI NaeI
NarI NcoI NgoMIV NheI NotI
NruI NsiI PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I RcaI
RsaI RsrII SacII SalI ScaI
SexAI SfiI SfoI SgrAI SmaI
SmlI SnaBI SpeI SphI SspBI
SspI SwaI Tru9I Tth111I Van91I
XbaI XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AccII 758 CGCG CG^CG
AflIII 63 ACATGT A^C[GA][CT]GT
AlwNI 1249 CAGACTCTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 1066 GAGCTC G[AT]GC[AT]^C
AvrII 993 CCTAGG C^CTAGG
BciVI 765 GTATCC GTATCC(6/5)
BglII 632 AGATCT A^GATCT
BlnI 993 CCTAGG C^CTAGG
BlpI 1207 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1207 GCTGAGC GC^T[ACGT]AGC
BsiHKAI 1066 GAGCTC G[AT]GC[AT]^C
BsmI 1179 GAATGC GAATGC(1/-1)
BspDI 364 ATCGAT AT^CGAT
BspLU11I 63 ACATGT A^CATGT
BssSI 1093 CACGAG CACGAG(-5/-1)
BstUI 758 CGCG CG^CG
BstXI 167 CCACCCTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 1207 GCTGAGC GC^T[ACGT]AGC
CfoI 757 GCGC GCG^C
ClaI 364 ATCGAT AT^CGAT
DdeI 1208 CTGAG C^T[ACGT]AG
DsaI 470 CCACGG C^C[GA][CT]GG
EcoRI 358 GAATTC G^AATTC
HhaI 757 GCGC GCG^C
HinP1I 757 GCGC G^CGC
HincII 841 GTCAAC GT[CT]^[GA]AC
HindII 841 GTCAAC GT[CT]^[GA]AC
MaeII 275 ACGT A^CGT
MaeIII 502 GTGAC ^GT[ACGT]AC
MslI 168 CACCCTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 758 CGCG CG^CG
NdeI 968 CATATG CA^TATG
NspI 63 ACATGT [GA]CATG^[CT]
PstI 388 CTGCAG CTGCA^G
PvuI 293 CGATCG CGAT^CG
PvuII 91 CAGCTG CAG^CTG
SacI 1066 GAGCTC GAGCT^C
SapI 394 GAAGAGC GCTCTTC(1/4)
SbfI 387 CCTGCAGG CCTGCA^GG
Sse8387I 387 CCTGCAGG CCTGCA^GG
StuI 380 AGGCCT AGG^CCT
Tsp45I 502 GTGAC ^GT[GC]AC
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# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AcsI 196 AAATTC [GA]^AATT[CT]
358 GAATTC
AlwI 405 GATCC GGATC(4/5)
852 GGATC
ApoI 196 AAATTC [GA]^AATT[CT]
358 GAATTC
Asp700I 984 GAACGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1179 GAATGCTTTC
AvaII 318 GGACC G^G[AT]CC
495 GGACC
BanII 1066 GAGCTC G[GA]GC[CT]^C
1227 GGGCTC
BcnI 229 CCGGG CC^[GC]GG
875 CCCGG
BfaI 499 CTAG C^TAG
994 CTAG
BseRI 910 GAGGAG GAGGAG(10/8)
1018 GAGGAG
BsiEI 8 CGACCG CG[GA][CT]^CG
293 CGATCG
BsrDI 462 CATTGC GCAATG(2/0)
546 CATTGC
BstBI 199 TTCGAA TT^CGAA
567 TTCGAA
EcoT14I 769 CCTTGG C^C[AT][AT]GG
993 CCTAGG
FauI 822 GCGGG CCCGC(4/6)
858 GCGGG
HapII 229 CCGG C^CGG
876 CCGG
HpaII 229 CCGG C^CGG
876 CCGG
MaeI 499 CTAG C^TAG
994 CTAG
MspA1I 91 CAGCTG C[AC]G^C[GT]G
897 CCGCTG
MspI 229 CCGG C^CGG
876 CCGG
NciI 229 CCGGG CC^[GC]GG
875 CCCGG
NspV 199 TTCGAA TT^CGAA
567 TTCGAA
SfcI 388 CTGCAG C^T[GA][CT]AG
647 CTACAG
SfuI 199 TTCGAA TT^CGAA
567 TTCGAA
StyI 769 CCTTGG C^C[AT][AT]GG
993 CCTAGG
TfiI 440 GAATC G^A[AT]TC
834 GATTC
TspRI 777 CACTG CA[GC]TG[ACGT][ACGT]^
802 CACTG
XmnI 984 GAACGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1179 GAATGCTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 49 enzymes) #
#########################################
AciI AluI BbvI BmyI BpmI
BsaJI BsgI BsiYI BslI BsmAI
BsmFI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI BstYI Cac8I Cfr13I
DpnII EarI EcoRII Fnu4HI FokI
HaeIII HinfI HphI ItaI Ksp632I
MboI MboII MflI MnlI MvaI
MwoI NdeII NlaIII NlaIV PleI
Sau3AI Sau96I ScrFI SfaNI TaqI
TseI Tsp509I TthHB8I XhoII