Restriction map for
pF1KA0130
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# NO CUT ENZYMES ( 78 enzymes) #
##################################
AatII Acc65I AclI AflII AgeI
AhdI Alw44I ApaLI AscI AseI
AsnI Asp718I AspEI AviII AvrII
BamHI BbeI BbrPI BciVI BfrI
BlnI BsaAI BsaBI BsiEI BsiWI
BspDI BssHII BssSI Bst1107I BstEII
BstPI BstZ17I ClaI DraI EagI
Eam1105I EclXI Eco52I EcoO65I EcoRV
FseI FspI HpaI KasI KpnI
KspI MamI MfeI MluI MunI
NarI NcoI NdeI NheI NotI
NruI PacI PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI SacII
SalI SapI SbfI SexAI SfiI
SfoI SgrAI SnaBI SpeI Sse8387I
SspI SwaI XbaI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AflIII 305 ACATGT A^C[GA][CT]GT
AocI 1600 CCTGAGG CC^T[ACGT]AGG
AspI 777 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbsI 2512 GAAGAC GAAGAC(2/6)
BcgI 2564 CGAAGTTGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1991 TGATCA T^GATCA
BglI 2428 GCCAAGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 851 AGATCT A^GATCT
BpuAI 2512 GAAGAC GAAGAC(2/6)
Bsp1407I 1075 TGTACA T^GTACA
BspHI 1994 TCATGA T^CATGA
BspLU11I 305 ACATGT A^CATGT
BsrGI 1075 TGTACA T^GTACA
BstBI 2562 TTCGAA TT^CGAA
Bsu36I 1600 CCTGAGG CC^T[ACGT]AGG
CpoI 1160 CGGACCG CG^G[AT]CCG
DraIII 733 CACGCCGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 271 GACCTGTGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 2817 CCGTGG C^C[GA][CT]GG
Eco81I 1600 CCTGAGG CC^T[ACGT]AGG
EcoRI 1417 GAATTC G^AATTC
EcoT22I 348 ATGCAT ATGCA^T
FbaI 1991 TGATCA T^GATCA
HindIII 860 AAGCTT A^AGCTT
MseI 2246 TTAA T^TAA
NaeI 442 GCCGGC GCC^GGC
NgoMIV 442 GCCGGC G^CCGGC
NsiI 348 ATGCAT ATGCA^T
NspV 2562 TTCGAA TT^CGAA
PaeR7I 748 CTCGAG C^TCGAG
PflFI 777 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 451 CCAGCCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 1994 TCATGA T^CATGA
RsrII 1160 CGGACCG CG^G[AT]CCG
ScaI 965 AGTACT AGT^ACT
SfuI 2562 TTCGAA TT^CGAA
SmaI 2412 CCCGGG CCC^GGG
SspBI 1075 TGTACA T^GTACA
Tru9I 2246 TTAA T^TAA
Tth111I 777 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 451 CCAGCCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 748 CTCGAG C^TCGAG
XmaCI 2412 CCCGGG C^CCGGG
XmaI 2412 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 29 enzymes) #
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AccI 2170 GTAGAC GT^[AC][GT]AC
2221 GTCTAC
AccIII 632 TCCGGA T^CCGGA
1883 TCCGGA
AcyI 2218 GGCGTC G[GA]^CG[CT]C
2481 GGCGTC
Aor51HI 47 AGCGCT AGC^GCT
1058 AGCGCT
ApaI 524 GGGCCC GGGCC^C
1466 GGGCCC
BalI 2702 TGGCCA TGG^CCA
2903 TGGCCA
BlpI 1147 GCTAAGC GC^T[ACGT]AGC
2580 GCTGAGC
Bpu1102I 1147 GCTAAGC GC^T[ACGT]AGC
2580 GCTGAGC
BsaHI 2218 GGCGTC G[GA]^CG[CT]C
2481 GGCGTC
BsaI 1576 GAGACC GGTCTC(1/5)
2738 GGTCTC
BseAI 632 TCCGGA T^CCGGA
1883 TCCGGA
BsmBI 775 GAGACG CGTCTC(1/5)
2263 GAGACG
BspEI 632 TCCGGA T^CCGGA
1883 TCCGGA
BspMI 1591 ACCTGC ACCTGC(4/8)
2096 ACCTGC
BsrBI 2178 CCGCTC CCGCTC(-3/-3)
418 GAGCGG
BsrDI 287 CATTGC GCAATG(2/0)
2955 CATTGC
CelII 1147 GCTAAGC GC^T[ACGT]AGC
2580 GCTGAGC
Eco47III 47 AGCGCT AGC^GCT
1058 AGCGCT
EcoT14I 2147 CCAAGG C^C[AT][AT]GG
2891 CCAAGG
Hin1I 2218 GGCGTC G[GA]^CG[CT]C
2481 GGCGTC
MaeII 1880 ACGT A^CGT
2027 ACGT
MluNI 2702 TGGCCA TGG^CCA
2903 TGGCCA
MroI 632 TCCGGA T^CCGGA
1883 TCCGGA
MscI 2702 TGGCCA TGG^CCA
2903 TGGCCA
PshAI 2337 GACCCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2477 GACAGGCGTC
SacI 904 GAGCTC GAGCT^C
2583 GAGCTC
StuI 554 AGGCCT AGG^CCT
1727 AGGCCT
StyI 2147 CCAAGG C^C[AT][AT]GG
2891 CCAAGG
TfiI 936 GATTC G^A[AT]TC
1533 GATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 107 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApoI Asp700I AspHI
AvaI AvaII BanI BanII BbvCI
BbvI BcnI BfaI BmyI BpmI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BsrFI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII EaeI EarI
EcoNI EcoO109I EcoRII FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MflI MnlI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SphI TaqI TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoII XmnI