Restriction map for
pF1KA0143
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# NO CUT ENZYMES ( 116 enzymes) #
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AatII Acc65I AccI AccII AccIII
AflII AflIII AgeI Alw44I AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspHI AspI AvrII
BanI BanII BbeI BbrPI BclI
BfrI BglI BlnI BlpI BmyI
Bpu1102I BsaAI BsaI BseAI BsiEI
BsiHKAI BsiWI Bsp1286I Bsp1407I BspDI
BspEI BspLU11I BsrBI BsrFI BsrGI
BssHII BssSI Bst1107I BstUI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DrdI DsaI EagI EclXI Eco47III
Eco52I Eco81I EcoNI EcoT14I FauI
FbaI FseI HaeII HpaI KasI
KpnI KspI MluI MroI MvnI
NaeI NarI NcoI NgoMIV NheI
NotI NruI PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI PstI PvuI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SgrAI
SmlI SnaBI SpeI Sse8387I SspBI
StuI StyI SwaI Tth111I XcmI
XhoI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AciI 29 CCGC CCGC(-3/-1)
AclI 1627 AACGTT AA^CGTT
AcyI 2243 GGCGTC G[GA]^CG[CT]C
AhdI 1427 GACAGTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 1427 GACAGTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 774 TGCGCA TGC^GCA
BalI 1721 TGGCCA TGG^CCA
BbvCI 571 CCTCAGC CCTCAGC(-5/-2)
BcgI 10 CGAGTATGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2107 GTATCC GTATCC(6/5)
BpmI 140 CTCCAG CTGGAG(16/14)
BsaBI 2065 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 2243 GGCGTC G[GA]^CG[CT]C
BsaWI 1506 ACCGGA [AT]^CCGG[AT]
BseRI 2363 CTCCTC GAGGAG(10/8)
BsmBI 1036 CGTCTC CGTCTC(1/5)
BsmI 1137 GAATGC GAATGC(1/-1)
BspMI 934 GCAGGT ACCTGC(4/8)
BstEII 1326 GGTGACC G^GT[ACGT]ACC
BstPI 1326 GGTGACC G^GT[ACGT]ACC
CfoI 775 GCGC GCG^C
DraI 829 TTTAAA TTT^AAA
DraII 645 AGGCCCT [GA]G^G[ACGT]CC[CT]
EaeI 1721 TGGCCA [CT]^GGCC[GA]
Eam1105I 1427 GACAGTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 679 GAAGAG CTCTTC(1/4)
EcoO109I 645 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 1326 GGTGACC G^GT[ACGT]ACC
EcoRV 1258 GATATC GAT^ATC
EcoT22I 1447 ATGCAT ATGCA^T
FspI 774 TGCGCA TGC^GCA
HhaI 775 GCGC GCG^C
Hin1I 2243 GGCGTC G[GA]^CG[CT]C
HinP1I 775 GCGC G^CGC
Ksp632I 679 GAAGAG CTCTTC(1/4)
MamI 2065 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 2201 CAATTG C^AATTG
MluNI 1721 TGGCCA TGG^CCA
MscI 1721 TGGCCA TGG^CCA
MspA1I 1585 CAGCTG C[AC]G^C[GT]G
MunI 2201 CAATTG C^AATTG
NsiI 1447 ATGCAT ATGCA^T
PleI 1999 GAGTC GAGTC(4/5)
Psp1406I 1627 AACGTT AA^CGTT
PvuII 1585 CAGCTG CAG^CTG
SfcI 1079 CTGTAG C^T[GA][CT]AG
SmaI 923 CCCGGG CCC^GGG
Tsp45I 1327 GTGAC ^GT[GC]AC
XbaI 2080 TCTAGA T^CTAGA
XmaCI 923 CCCGGG C^CCGGG
XmaI 923 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 24 enzymes) #
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AlwNI 131 CAGTATCTG CAG[ACGT][ACGT][ACGT]^CTG
946 CAGGTTCTG
AvaI 8 CCCGAG C^[CT]CG[GA]G
923 CCCGGG
AvaII 243 GGACC G^G[AT]CC
991 GGTCC
BamHI 801 GGATCC G^GATCC
1383 GGATCC
BcnI 923 CCCGG CC^[GC]GG
924 CCGGG
BglII 1312 AGATCT A^GATCT
2448 AGATCT
BsgI 1361 CTGCAC GTGCAG(16/14)
1132 GTGCAG
BsmFI 1840 GTCCC GGGAC(10/14)
2422 GTCCC
BsoBI 8 CCCGAG C^[CT]CG[GA]G
923 CCCGGG
BsrDI 969 CATTGC GCAATG(2/0)
1725 CATTGC
BstAPI 130 GCAGTATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1795 GCACTGGTTGC
BstBI 1051 TTCGAA TT^CGAA
1880 TTCGAA
DraIII 868 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
2294 CACAGTGTG
EcoRI 1048 GAATTC G^AATTC
1292 GAATTC
HapII 924 CCGG C^CGG
1507 CCGG
HpaII 924 CCGG C^CGG
1507 CCGG
MspI 924 CCGG C^CGG
1507 CCGG
NciI 923 CCCGG CC^[GC]GG
924 CCGGG
NdeI 304 CATATG CA^TATG
577 CATATG
NspV 1051 TTCGAA TT^CGAA
1880 TTCGAA
SfaNI 901 GATGC GCATC(5/9)
916 GATGC
SfuI 1051 TTCGAA TT^CGAA
1880 TTCGAA
SphI 1934 GCATGC GCATG^C
2403 GCATGC
SspI 382 AATATT AAT^ATT
2340 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
Asp700I BbsI BbvI BfaI BpuAI
BsaJI BsiYI BslI BsmAI BspHI
BsrI BssKI BstF5I BstNI BstXI
BstYI Cac8I Cfr13I DdeI DpnII
EcoRII Fnu4HI FokI HaeIII HgaI
HincII HindII HindIII HinfI HphI
ItaI MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MvaI MwoI NdeII NlaIII NlaIV
NspI PflMI RcaI RsaI Sau3AI
Sau96I ScrFI TaqI TfiI Tru9I
TseI Tsp509I TspRI TthHB8I Van91I
XhoII XmnI