Restriction map for
pF1KA0144
##################################
# NO CUT ENZYMES ( 95 enzymes) #
##################################
AatII AccII AccIII AclI AcsI
AcyI AflII AflIII AgeI AocI
Aor51HI ApoI AscI AseI AsnI
AspI AviII BbeI BbrPI BcgI
BclI BfrI BglI BsaBI BsaHI
BsaWI BseAI BsiWI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrGI BssHII
BstAPI BstUI Bsu36I ClaI CpoI
DraI DraIII DrdI Eco47III Eco81I
EcoRI FbaI FseI FspI Hin1I
HindIII HpaI KasI KspI MamI
MluI MroI MvnI NarI NcoI
NdeI NheI NotI NruI PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SapI SbfI
SexAI SfiI SfoI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Tth111I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
Acc65I 1802 GGTACC G^GTACC
AhdI 1414 GACAACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1240 GTGCAC G^TGCAC
ApaI 1764 GGGCCC GGGCC^C
ApaLI 1240 GTGCAC G^TGCAC
Asp718I 1802 GGTACC G^GTACC
AspEI 1414 GACAACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 1240 GTGCAC G[AT]GC[AT]^C
AvrII 2295 CCTAGG C^CTAGG
BamHI 906 GGATCC G^GATCC
BciVI 2890 GGATAC GTATCC(6/5)
BlnI 2295 CCTAGG C^CTAGG
BsaAI 660 TACGTG [CT]AC^GT[GA]
BsiEI 2595 CGGCCG CG[GA][CT]^CG
BsiHKAI 1240 GTGCAC G[AT]GC[AT]^C
BsmBI 911 CGTCTC CGTCTC(1/5)
BsrBI 1716 GAGCGG CCGCTC(-3/-3)
BsrFI 1437 GCCGGC [GA]^CCGG[CT]
BssSI 1611 CACGAG CACGAG(-5/-1)
Bst1107I 1647 GTATAC GTA^TAC
BstBI 954 TTCGAA TT^CGAA
BstEII 2578 GGTGACC G^GT[ACGT]ACC
BstPI 2578 GGTGACC G^GT[ACGT]ACC
BstZ17I 1647 GTATAC GTA^TAC
Cfr10I 1437 GCCGGC [GA]^CCGG[CT]
EagI 2595 CGGCCG C^GGCCG
Eam1105I 1414 GACAACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 2595 CGGCCG C^GGCCG
Eco52I 2595 CGGCCG C^GGCCG
EcoO65I 2578 GGTGACC G^GT[ACGT]ACC
EcoRV 1528 GATATC GAT^ATC
EcoT22I 2843 ATGCAT ATGCA^T
HgaI 2658 GACGC GACGC(5/10)
KpnI 1802 GGTACC GGTAC^C
MfeI 166 CAATTG C^AATTG
MseI 596 TTAA T^TAA
MunI 166 CAATTG C^AATTG
NaeI 1437 GCCGGC GCC^GGC
NgoMIV 1437 GCCGGC G^CCGGC
NsiI 2843 ATGCAT ATGCA^T
NspV 954 TTCGAA TT^CGAA
PshAI 385 GACTATAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SalI 1731 GTCGAC G^TCGAC
ScaI 1066 AGTACT AGT^ACT
SfuI 954 TTCGAA TT^CGAA
Tru9I 596 TTAA T^TAA
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccI 1647 GTATAC GT^[AC][GT]AC
1731 GTCGAC
AfaI 1067 GTAC GT^AC
1803 GTAC
Asp700I 566 GAAGGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1854 GAATGGCTTC
AvaI 29 CCCGGG C^[CT]CG[GA]G
2751 CCCGGG
BbvCI 1432 CCTCAGC CCTCAGC(-5/-2)
2289 CCTCAGC
BglII 753 AGATCT A^GATCT
770 AGATCT
BsoBI 29 CCCGGG C^[CT]CG[GA]G
2751 CCCGGG
BspMI 1317 ACCTGC ACCTGC(4/8)
1544 ACCTGC
BsrDI 1559 CATTGC GCAATG(2/0)
224 GCAATG
BstXI 321 CCAGAAGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2627 CCACAACCTTGG
EcoNI 979 CCTGCTTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1839 CCTGACTCAGG
FauI 2385 CCCGC CCCGC(4/6)
2439 CCCGC
HincII 235 GTCAAC GT[CT]^[GA]AC
1731 GTCGAC
HindII 235 GTCAAC GT[CT]^[GA]AC
1731 GTCGAC
NspI 1019 GCATGT [GA]CATG^[CT]
2430 ACATGC
PvuII 602 CAGCTG CAG^CTG
1337 CAGCTG
RsaI 1067 GTAC GT^AC
1803 GTAC
SmaI 29 CCCGGG CCC^GGG
2751 CCCGGG
SmlI 578 CTCAAG C^T[CT][GA]AG
2034 CTTGAG
XcmI 321 CCAGAAGGATGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2862 CCAACAGCCGAGTGG
XmaCI 29 CCCGGG C^CCGGG
2751 CCCGGG
XmaI 29 CCCGGG C^CCGGG
2751 CCCGGG
XmnI 566 GAAGGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1854 GAATGGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 94 enzymes) #
#########################################
AciI AluI AlwI AlwNI AvaII
BalI BanI BanII BbsI BbvI
BcnI BfaI BlpI BmyI BpmI
Bpu1102I BpuAI BsaI BsaJI BseRI
BsgI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I BsrI BssKI BstF5I
BstNI BstYI Cac8I CelII CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PflMI PleI PpuMI PstI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TaqI TfiI TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XhoII