Restriction map for
pF1KA0148
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# NO CUT ENZYMES ( 89 enzymes) #
##################################
AccI AgeI Alw44I AlwNI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I AspI AviII AvrII BbrPI
BbvCI BcgI BclI BglI BlnI
BsaBI BsgI Bsp1407I BspDI BspHI
BspLU11I BsrBI BsrDI BsrGI BssHII
Bst1107I BstZ17I ClaI CpoI DraI
DrdI EagI EclXI Eco47III Eco52I
EcoRV EcoT22I FbaI FseI FspI
HpaI KspI MamI MfeI MluI
MunI NaeI NdeI NgoMIV NheI
NotI NruI NsiI NspI PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshBI PvuI RcaI RsrII
SacII SalI SapI SbfI SexAI
SfiI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tth111I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 63 enzymes) #
###################################
Acc65I 1632 GGTACC G^GTACC
AccII 1491 CGCG CG^CG
AccIII 14 TCCGGA T^CCGGA
AclI 81 AACGTT AA^CGTT
AflII 1737 CTTAAG C^TTAAG
AflIII 1794 ACACGT A^C[GA][CT]GT
AhdI 244 GACCCTGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 1278 CCTCAGG CC^T[ACGT]AGG
Asp718I 1632 GGTACC G^GTACC
AspEI 244 GACCCTGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 1615 TGGCCA TGG^CCA
BamHI 51 GGATCC G^GATCC
BbeI 125 GGCGCC GGCGC^C
BciVI 1790 GGATAC GTATCC(6/5)
BfrI 1737 CTTAAG C^TTAAG
BglII 393 AGATCT A^GATCT
BlpI 768 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 768 GCTCAGC GC^T[ACGT]AGC
BsaAI 1795 CACGTA [CT]AC^GT[GA]
BseAI 14 TCCGGA T^CCGGA
BseRI 1068 GAGGAG GAGGAG(10/8)
BsiEI 941 CGGTCG CG[GA][CT]^CG
BsiWI 1982 CGTACG C^GTACG
BsmI 231 GCATTC GAATGC(1/-1)
BspEI 14 TCCGGA T^CCGGA
BspMI 1965 GCAGGT ACCTGC(4/8)
BsrFI 1238 ACCGGC [GA]^CCGG[CT]
BssSI 655 CTCGTG CACGAG(-5/-1)
BstBI 1272 TTCGAA TT^CGAA
BstUI 1491 CGCG CG^CG
Bsu36I 1278 CCTCAGG CC^T[ACGT]AGG
CelII 768 GCTCAGC GC^T[ACGT]AGC
Cfr10I 1238 ACCGGC [GA]^CCGG[CT]
DraII 1925 GGGACCC [GA]G^G[ACGT]CC[CT]
DraIII 159 CACACCGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 1615 TGGCCA [CT]^GGCC[GA]
Eam1105I 244 GACCCTGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1278 CCTCAGG CC^T[ACGT]AGG
EcoNI 490 CCTGAAAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 1925 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoRI 313 GAATTC G^AATTC
HincII 871 GTTGAC GT[CT]^[GA]AC
HindII 871 GTTGAC GT[CT]^[GA]AC
HindIII 1246 AAGCTT A^AGCTT
KasI 125 GGCGCC G^GCGCC
KpnI 1632 GGTACC GGTAC^C
MluNI 1615 TGGCCA TGG^CCA
MroI 14 TCCGGA T^CCGGA
MscI 1615 TGGCCA TGG^CCA
MvnI 1491 CGCG CG^CG
NarI 125 GGCGCC GG^CGCC
NcoI 851 CCATGG C^CATGG
NspV 1272 TTCGAA TT^CGAA
PpuMI 1925 GGGACCC [GA]G^G[AT]CC[CT]
Psp1406I 81 AACGTT AA^CGTT
ScaI 971 AGTACT AGT^ACT
SfoI 125 GGCGCC GGC^GCC
SfuI 1272 TTCGAA TT^CGAA
SmaI 1376 CCCGGG CCC^GGG
SmlI 1737 CTTAAG C^T[CT][GA]AG
XcmI 1838 CCAAGTCTGATATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 1376 CCCGGG C^CCGGG
XmaI 1376 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AatII 1364 GACGTC GACGT^C
1875 GACGTC
AcsI 313 GAATTC [GA]^AATT[CT]
1269 AAATTC
ApoI 313 GAATTC [GA]^AATT[CT]
1269 AAATTC
AvaI 1092 CTCGGG C^[CT]CG[GA]G
1376 CCCGGG
AvaII 243 GGACC G^G[AT]CC
1926 GGACC
BanII 1114 GAGCTC G[GA]GC[CT]^C
1732 GAGCTC
BsaI 193 GAGACC GGTCTC(1/5)
1405 GAGACC
BsmBI 883 GAGACG CGTCTC(1/5)
1362 GAGACG
BsoBI 1092 CTCGGG C^[CT]CG[GA]G
1376 CCCGGG
BstAPI 231 GCATTCTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
793 GCAAAAGCTGC
BstEII 927 GGTAACC G^GT[ACGT]ACC
2013 GGTTACC
BstPI 927 GGTAACC G^GT[ACGT]ACC
2013 GGTTACC
BstXI 1553 CCAACGACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2003 CCAAGCAGCTGG
BstYI 51 GGATCC [GA]^GATC[CT]
393 AGATCT
DsaI 163 CCGTGG C^C[GA][CT]GG
851 CCATGG
EarI 1350 CTCTTC CTCTTC(1/4)
1459 GAAGAG
EcoO65I 927 GGTAACC G^GT[ACGT]ACC
2013 GGTTACC
EcoT14I 851 CCATGG C^C[AT][AT]GG
1994 CCAAGG
FauI 1489 CCCGC CCCGC(4/6)
44 GCGGG
HgaI 1060 GACGC GACGC(5/10)
250 GCGTC
Ksp632I 1350 CTCTTC CTCTTC(1/4)
1459 GAAGAG
MflI 51 GGATCC [GA]^GATC[CT]
393 AGATCT
MseI 1357 TTAA T^TAA
1738 TTAA
PflMI 636 CCATGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1618 CCAGGGGATGG
PvuII 1951 CAGCTG CAG^CTG
2008 CAGCTG
SacI 1114 GAGCTC GAGCT^C
1732 GAGCTC
StyI 851 CCATGG C^C[AT][AT]GG
1994 CCAAGG
TfiI 432 GAATC G^A[AT]TC
589 GATTC
Tru9I 1357 TTAA T^TAA
1738 TTAA
Van91I 636 CCATGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1618 CCAGGGGATGG
XhoII 51 GGATCC [GA]^GATC[CT]
393 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 75 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AspHI BanI BbsI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaHI
BsaJI BsaWI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrI BssKI
BstF5I BstNI Cac8I CfoI Cfr13I
DdeI DpnII EcoRII Fnu4HI FokI
HaeII HaeIII HapII HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
MaeI MaeII MaeIII MboI MboII
MnlI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PleI PshAI PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI TaqI
TseI Tsp45I Tsp509I TspRI TthHB8I