Restriction map for
pF1KA0231
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# NO CUT ENZYMES ( 106 enzymes) #
##################################
Acc65I AccIII AflII AflIII AgeI
AhdI Alw44I ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AviII
AvrII BanI BbeI BbrPI BcgI
BciVI BfrI BglII BlnI BsaAI
BsaBI BsaI BsaWI BseAI BsiEI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I Cfr10I
ClaI CpoI DraIII DrdI EagI
Eam1105I EclXI Eco52I EcoO65I EcoRV
FseI FspI KasI KpnI KspI
MamI MfeI MluI MroI MunI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NspV PacI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI PvuI RcaI RsrII SacII
SalI SbfI SexAI SfoI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SspBI SwaI Van91I XcmI XmaCI
XmaI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AatII 73 GACGTC GACGT^C
AccI 1429 GTAGAC GT^[AC][GT]AC
AccII 499 CGCG CG^CG
AclI 2381 AACGTT AA^CGTT
AcyI 73 GACGTC G[GA]^CG[CT]C
Aor51HI 899 AGCGCT AGC^GCT
AspI 1300 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 445 CTCGAG C^[CT]CG[GA]G
BalI 458 TGGCCA TGG^CCA
BamHI 1652 GGATCC G^GATCC
BbvCI 1011 GCTGAGG CCTCAGC(-5/-2)
BcnI 1649 CCCGG CC^[GC]GG
BglI 115 GCCGTGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI 73 GACGTC G[GA]^CG[CT]C
BsmAI 1375 GTCTC GTCTC(1/5)
BsoBI 445 CTCGAG C^[CT]CG[GA]G
BsrBI 262 CCGCTC CCGCTC(-3/-3)
BstUI 499 CGCG CG^CG
BstXI 668 CCAGTGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraI 1464 TTTAAA TTT^AAA
Eco47III 899 AGCGCT AGC^GCT
EcoNI 1161 CCTATCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 275 GAATTC G^AATTC
EcoT22I 736 ATGCAT ATGCA^T
FauI 497 CCCGC CCCGC(4/6)
HaeII 899 AGCGCT [GA]GCGC^[CT]
HgaI 141 GACGC GACGC(5/10)
Hin1I 73 GACGTC G[GA]^CG[CT]C
HindIII 1237 AAGCTT A^AGCTT
HpaI 1314 GTTAAC GTT^AAC
MluNI 458 TGGCCA TGG^CCA
MscI 458 TGGCCA TGG^CCA
MvnI 499 CGCG CG^CG
NciI 1649 CCCGG CC^[GC]GG
NsiI 736 ATGCAT ATGCA^T
PaeR7I 445 CTCGAG C^TCGAG
PflFI 1300 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 640 GAGTC GAGTC(4/5)
Psp1406I 2381 AACGTT AA^CGTT
SapI 1635 GAAGAGC GCTCTTC(1/4)
SfiI 114 GGCCGTGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI 734 GCATGC GCATG^C
StuI 535 AGGCCT AGG^CCT
Tth111I 1300 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 1452 TCTAGA T^CTAGA
XhoI 445 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AocI 575 CCTCAGG CC^T[ACGT]AGG
1842 CCTAAGG
Asp700I 723 GAAAAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2281 GAAACACTTC
BanII 128 GAGCTC G[GA]GC[CT]^C
2260 GAGCTC
BstYI 1652 GGATCC [GA]^GATC[CT]
2052 GGATCT
Bsu36I 575 CCTCAGG CC^T[ACGT]AGG
1842 CCTAAGG
CfoI 500 GCGC GCG^C
900 GCGC
DraII 938 AGGTCCT [GA]G^G[ACGT]CC[CT]
1621 AGGACCC
DsaI 64 CCATGG C^C[GA][CT]GG
487 CCATGG
Eco81I 575 CCTCAGG CC^T[ACGT]AGG
1842 CCTAAGG
EcoO109I 938 AGGTCCT [GA]G^G[ACGT]CC[CT]
1621 AGGACCC
HhaI 500 GCGC GCG^C
900 GCGC
HinP1I 500 GCGC G^CGC
900 GCGC
HincII 1314 GTTAAC GT[CT]^[GA]AC
1387 GTCAAC
HindII 1314 GTTAAC GT[CT]^[GA]AC
1387 GTCAAC
MflI 1652 GGATCC [GA]^GATC[CT]
2052 GGATCT
MslI 735 CATGCATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2227 CATGTGGGTG
NcoI 64 CCATGG C^CATGG
487 CCATGG
NlaIV 1622 GGACCC GG[ACGT]^[ACGT]CC
1652 GGATCC
NspI 734 GCATGC [GA]CATG^[CT]
738 GCATGT
PpuMI 938 AGGTCCT [GA]G^G[AT]CC[CT]
1621 AGGACCC
SacI 128 GAGCTC GAGCT^C
2260 GAGCTC
XhoII 1652 GGATCC [GA]^GATC[CT]
2052 GGATCT
XmnI 723 GAAAAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2281 GAAACACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 83 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AspHI AvaII BbsI
BbvI BclI BfaI BlpI BmyI
BpmI Bpu1102I BpuAI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmFI
Bsp1286I BsrDI BsrI BssKI BstF5I
BstNI Cac8I CelII Cfr13I DdeI
DpnII EaeI EarI EcoRII EcoT14I
FbaI Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MnlI MseI MspA1I MspI MvaI
MwoI NdeII NlaIII PstI PvuII
RsaI Sau3AI Sau96I ScaI ScrFI
SfaNI SfcI SmlI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I