Restriction map for
pF1KA0232
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# NO CUT ENZYMES ( 106 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AgeI AhdI Alw44I Aor51HI
ApaLI AscI Asp718I AspEI AspI
AviII AvrII BalI BbeI BbrPI
BcnI BglI BlnI BlpI Bpu1102I
BsaAI BsaHI BseAI BsiEI BsiWI
BsmBI BspDI BspEI BspLU11I BsrBI
BsrFI BssHII Bst1107I BstAPI BstEII
BstPI BstZ17I CelII Cfr10I ClaI
CpoI DraIII DrdI EaeI EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FseI
FspI HgaI Hin1I HindIII HpaI
KasI KpnI MluI MluNI MroI
MscI NaeI NarI NciI NdeI
NgoMIV NotI NruI NsiI NspI
PacI PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI Psp1406I PvuI
RsrII SalI ScaI SfiI SfoI
SgrAI SmaI SnaBI SpeI SphI
StuI SwaI Tth111I Van91I XmaCI
XmaI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AccII 4075 CGCG CG^CG
AflII 3129 CTTAAG C^TTAAG
AflIII 2158 ACACGT A^C[GA][CT]GT
AocI 664 CCTAAGG CC^T[ACGT]AGG
ApaI 4146 GGGCCC GGGCC^C
AvaI 139 CTCGAG C^[CT]CG[GA]G
BamHI 1557 GGATCC G^GATCC
BanI 1564 GGTGCC G^G[CT][GA]CC
BbvCI 1726 GCTGAGG CCTCAGC(-5/-2)
BcgI 1212 CGAGCCAATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1284 GGATAC GTATCC(6/5)
BfrI 3129 CTTAAG C^TTAAG
BsaWI 1792 TCCGGT [AT]^CCGG[AT]
BsmI 3163 GAATGC GAATGC(1/-1)
BsoBI 139 CTCGAG C^[CT]CG[GA]G
BspHI 572 TCATGA T^CATGA
BsrDI 1959 GCAATG GCAATG(2/0)
BstBI 965 TTCGAA TT^CGAA
BstUI 4075 CGCG CG^CG
BstXI 3486 CCATTACTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 664 CCTAAGG CC^T[ACGT]AGG
Eco81I 664 CCTAAGG CC^T[ACGT]AGG
FauI 1092 GCGGG CCCGC(4/6)
HapII 1793 CCGG C^CGG
HincII 3274 GTTGAC GT[CT]^[GA]AC
HindII 3274 GTTGAC GT[CT]^[GA]AC
HpaII 1793 CCGG C^CGG
KspI 4074 CCGCGG CCGC^GG
MspI 1793 CCGG C^CGG
MvnI 4075 CGCG CG^CG
NcoI 2137 CCATGG C^CATGG
NheI 643 GCTAGC G^CTAGC
NspV 965 TTCGAA TT^CGAA
PaeR7I 139 CTCGAG C^TCGAG
RcaI 572 TCATGA T^CATGA
SacII 4074 CCGCGG CCGC^GG
SexAI 3856 ACCTGGT A^CC[AT]GGT
SfuI 965 TTCGAA TT^CGAA
XcmI 112 CCAGTGCAGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 139 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AlwNI 65 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG
2885 CAGCTTCTG
BclI 1542 TGATCA T^GATCA
2997 TGATCA
BglII 303 AGATCT A^GATCT
3447 AGATCT
BsaBI 1498 GATATTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1537 GATATTGATC
BsgI 115 GTGCAG GTGCAG(16/14)
2882 GTGCAG
BssSI 956 CACGAG CACGAG(-5/-1)
858 CTCGTG
CfoI 3987 GCGC GCG^C
4094 GCGC
DraI 1107 TTTAAA TTT^AAA
3208 TTTAAA
DsaI 2137 CCATGG C^C[GA][CT]GG
4074 CCGCGG
FbaI 1542 TGATCA T^GATCA
2997 TGATCA
HaeII 3986 AGCGCC [GA]GCGC^[CT]
4093 AGCGCC
HhaI 3987 GCGC GCG^C
4094 GCGC
HinP1I 3987 GCGC G^CGC
4094 GCGC
MamI 1498 GATATTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1537 GATATTGATC
MfeI 788 CAATTG C^AATTG
1217 CAATTG
MunI 788 CAATTG C^AATTG
1217 CAATTG
PpuMI 1453 GGGACCT [GA]G^G[AT]CC[CT]
1745 AGGACCT
PvuII 1081 CAGCTG CAG^CTG
3790 CAGCTG
SapI 3982 GAAGAGC GCTCTTC(1/4)
614 GCTCTTC
SbfI 219 CCTGCAGG CCTGCA^GG
1740 CCTGCAGG
Sse8387I 219 CCTGCAGG CCTGCA^GG
1740 CCTGCAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 91 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AseI AsnI Asp700I AspHI
AvaII BanII BbsI BbvI BfaI
BmyI BpmI BpuAI BsaI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I Bsp1407I BspMI BsrGI
BsrI BssKI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII DraII
EarI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeIII HinfI HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MvaI MwoI NdeII NlaIII
NlaIV PleI PshBI PstI RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspBI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XbaI XhoII
XmnI