Restriction map for
pF1KA0244
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# NO CUT ENZYMES ( 77 enzymes) #
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AatII AccII AccIII AgeI AocI
ApaI AscI AseI AsnI AvaI
AviII BanII BseAI BsiEI BsiWI
BsoBI BspDI BspEI BsrBI BsrFI
BssHII Bst1107I BstEII BstPI BstUI
BstXI BstZ17I Bsu36I Cfr10I ClaI
CpoI DrdI EagI EclXI Eco52I
Eco81I EcoO65I FseI FspI HpaI
KspI MfeI MluI MroI MspA1I
MunI MvnI NaeI NdeI NgoMIV
NotI NruI PacI PaeR7I PinAI
PmeI PshAI PshBI PvuI PvuII
RsrII SacI SacII SalI SbfI
SfiI SgrAI SmaI SnaBI SphI
Sse8387I StuI SwaI XbaI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AclI 3543 AACGTT AA^CGTT
AcyI 5684 GGCGCC G[GA]^CG[CT]C
AflII 339 CTTAAG C^TTAAG
AhdI 3295 GACTCTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 3887 AGCGCT AGC^GCT
Asp700I 4255 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 3295 GACTCTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 4970 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 4954 CCTAGG C^CTAGG
BalI 5563 TGGCCA TGG^CCA
BbeI 5684 GGCGCC GGCGC^C
BbrPI 1924 CACGTG CAC^GTG
BcgI 5253 GCACCCATTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1134 GGATAC GTATCC(6/5)
BcnI 727 CCGGG CC^[GC]GG
BfrI 339 CTTAAG C^TTAAG
BlnI 4954 CCTAGG C^CTAGG
BlpI 144 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 144 GCTCAGC GC^T[ACGT]AGC
BsaAI 1924 CACGTG [CT]AC^GT[GA]
BsaBI 151 GATAAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 5684 GGCGCC G[GA]^CG[CT]C
BsaI 4922 GGTCTC GGTCTC(1/5)
BsaWI 1290 ACCGGA [AT]^CCGG[AT]
BsmBI 3078 GAGACG CGTCTC(1/5)
BsmI 1353 GAATGC GAATGC(1/-1)
BspMI 3918 GCAGGT ACCTGC(4/8)
BssSI 5121 CTCGTG CACGAG(-5/-1)
BstBI 5261 TTCGAA TT^CGAA
CelII 144 GCTCAGC GC^T[ACGT]AGC
DraIII 5924 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 3295 GACTCTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 3887 AGCGCT AGC^GCT
EcoRV 3744 GATATC GAT^ATC
EcoT22I 3470 ATGCAT ATGCA^T
Hin1I 5684 GGCGCC G[GA]^CG[CT]C
KasI 5684 GGCGCC G^GCGCC
MamI 151 GATAAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 5563 TGGCCA TGG^CCA
MscI 5563 TGGCCA TGG^CCA
NarI 5684 GGCGCC GG^CGCC
NciI 727 CCGGG CC^[GC]GG
NheI 2556 GCTAGC G^CTAGC
NsiI 3470 ATGCAT ATGCA^T
NspV 5261 TTCGAA TT^CGAA
PflFI 4970 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1924 CACGTG CAC^GTG
PmlI 1924 CACGTG CAC^GTG
Psp1406I 3543 AACGTT AA^CGTT
SapI 1933 GAAGAGC GCTCTTC(1/4)
SfoI 5684 GGCGCC GGC^GCC
SfuI 5261 TTCGAA TT^CGAA
Tth111I 4970 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 3242 CCAATAAGTCATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 4255 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AflIII 1851 ACATGT A^C[GA][CT]GT
5308 ACATGT
Alw44I 4109 GTGCAC G^TGCAC
5251 GTGCAC
ApaLI 4109 GTGCAC G^TGCAC
5251 GTGCAC
BbvCI 1888 CCTCAGC CCTCAGC(-5/-2)
4237 CCTCAGC
BclI 2106 TGATCA T^GATCA
3021 TGATCA
BglI 4415 GCCTTTTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
5676 GCCAGAAAGGC
BglII 370 AGATCT A^GATCT
4849 AGATCT
BpmI 5525 CTCCAG CTGGAG(16/14)
76 CTGGAG
BsgI 1838 CTGCAC GTGCAG(16/14)
597 GTGCAG
Bsp1407I 1048 TGTACA T^GTACA
2736 TGTACA
BspHI 750 TCATGA T^CATGA
2685 TCATGA
BspLU11I 1851 ACATGT A^CATGT
5308 ACATGT
BsrGI 1048 TGTACA T^GTACA
2736 TGTACA
BstAPI 2866 GCAAAAGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3353 GCAAAATCTGC
CfoI 3888 GCGC GCG^C
5685 GCGC
EaeI 3142 CGGCCA [CT]^GGCC[GA]
5563 TGGCCA
EcoNI 160 CCTATGCTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3702 CCTGACAGAGG
EcoRI 2374 GAATTC G^AATTC
3505 GAATTC
FauI 2015 CCCGC CCCGC(4/6)
5640 CCCGC
FbaI 2106 TGATCA T^GATCA
3021 TGATCA
HaeII 3887 AGCGCT [GA]GCGC^[CT]
5684 GGCGCC
HgaI 1012 GACGC GACGC(5/10)
5840 GACGC
HhaI 3888 GCGC GCG^C
5685 GCGC
HinP1I 3888 GCGC G^CGC
5685 GCGC
PflMI 3679 CCAGTATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5527 CCAGGCTTTGG
RcaI 750 TCATGA T^CATGA
2685 TCATGA
SexAI 2676 ACCAGGT A^CC[AT]GGT
5654 ACCAGGT
SspBI 1048 TGTACA T^GTACA
2736 TGTACA
Van91I 3679 CCAGTATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5527 CCAGGCTTTGG
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# 3 OR MORE CUT ENZYMES ( 97 enzymes) #
#########################################
Acc65I AccI AciI AcsI AfaI
AluI AlwI AlwNI ApoI Asp718I
AspHI AvaII BamHI BanI BbsI
BbvI BfaI BmyI BpuAI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrDI BsrI BssKI
BstF5I BstNI BstYI Cac8I Cfr13I
DdeI DpnII DraI DraII DsaI
EarI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HincII HindII
HindIII HinfI HpaII HphI ItaI
KpnI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspI MvaI MwoI NcoI
NdeII NlaIII NlaIV NspI PleI
PpuMI PstI RsaI Sau3AI Sau96I
ScaI ScrFI SfaNI SfcI SmlI
SpeI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII