Restriction map for
pF1KA0267
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# NO CUT ENZYMES ( 124 enzymes) #
##################################
AatII Acc65I AccI AclI AflII
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaLI AscI AseI AsnI
Asp718I AspEI AspHI AviII AvrII
BamHI BbeI BbrPI BbsI BbvCI
BciVI BfrI BglI BglII BlnI
BlpI Bpu1102I BpuAI BsaI BsiEI
BsiHKAI BsiWI BsmBI Bsp1407I BspDI
BspHI BspLU11I BsrBI BsrGI BssHII
Bst1107I BstEII BstPI BstZ17I Bsu36I
CelII ClaI CpoI DraI DraII
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRV EcoT22I FseI
FspI HincII HindII HindIII HpaI
KasI KpnI KspI MfeI MluI
MunI NaeI NarI NgoMIV NheI
NotI NruI NsiI NspI PacI
PaeR7I PinAI PmaCI PmeI PmlI
PpuMI PshBI Psp1406I PstI PvuI
RcaI RsrII SacI SacII SalI
SbfI ScaI SexAI SfcI SfiI
SfoI SgrAI SmaI SmlI SpeI
SphI Sse8387I SspBI SwaI XbaI
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AccII 10 CGCG CG^CG
AccIII 1604 TCCGGA T^CCGGA
ApaI 284 GGGCCC GGGCC^C
Asp700I 1865 GAAACAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 1994 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 56 CCCGAG C^[CT]CG[GA]G
BalI 1394 TGGCCA TGG^CCA
BcgI 118 GCAGGGGCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1623 TGATCA T^GATCA
BcnI 282 CCGGG CC^[GC]GG
BsaAI 1095 TACGTA [CT]AC^GT[GA]
BsaBI 1762 GATTCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1604 TCCGGA [AT]^CCGG[AT]
BseAI 1604 TCCGGA T^CCGGA
BsmI 356 GCATTC GAATGC(1/-1)
BsoBI 56 CCCGAG C^[CT]CG[GA]G
BspEI 1604 TCCGGA T^CCGGA
BssSI 718 CACGAG CACGAG(-5/-1)
BstBI 518 TTCGAA TT^CGAA
BstUI 10 CGCG CG^CG
BstXI 819 CCAGCCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
FbaI 1623 TGATCA T^GATCA
MaeII 1096 ACGT A^CGT
MamI 1762 GATTCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1394 TGGCCA TGG^CCA
MroI 1604 TCCGGA T^CCGGA
MscI 1394 TGGCCA TGG^CCA
MvnI 10 CGCG CG^CG
NciI 282 CCGGG CC^[GC]GG
NcoI 155 CCATGG C^CATGG
NdeI 1802 CATATG CA^TATG
NspV 518 TTCGAA TT^CGAA
PflFI 1994 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 1117 GAGTC GAGTC(4/5)
PshAI 832 GACAACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 824 CAGCTG CAG^CTG
SfuI 518 TTCGAA TT^CGAA
SnaBI 1095 TACGTA TAC^GTA
SspI 1282 AATATT AAT^ATT
StuI 990 AGGCCT AGG^CCT
Tth111I 1994 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 1865 GAAACAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 25 enzymes) #
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AcyI 40 GGCGTC G[GA]^CG[CT]C
103 GGCGTC
AfaI 1016 GTAC GT^AC
1614 GTAC
AlwNI 683 CAGCAACTG CAG[ACGT][ACGT][ACGT]^CTG
695 CAGTGACTG
BanII 59 GAGCCC G[GA]GC[CT]^C
284 GGGCCC
BfaI 149 CTAG C^TAG
538 CTAG
BsaHI 40 GGCGTC G[GA]^CG[CT]C
103 GGCGTC
BsgI 295 CTGCAC GTGCAG(16/14)
403 GTGCAG
BspMI 218 ACCTGC ACCTGC(4/8)
487 GCAGGT
BstYI 878 GGATCT [GA]^GATC[CT]
901 GGATCT
DdeI 391 CTGAG C^T[ACGT]AG
1121 CTCAG
EaeI 1394 TGGCCA [CT]^GGCC[GA]
1832 CGGCCA
EarI 268 CTCTTC CTCTTC(1/4)
1271 GAAGAG
HaeII 211 AGCGCC [GA]GCGC^[CT]
1843 AGCGCC
HgaI 41 GCGTC GACGC(5/10)
104 GCGTC
Hin1I 40 GGCGTC G[GA]^CG[CT]C
103 GGCGTC
Ksp632I 268 CTCTTC CTCTTC(1/4)
1271 GAAGAG
MaeI 149 CTAG C^TAG
538 CTAG
MflI 878 GGATCT [GA]^GATC[CT]
901 GGATCT
MseI 1226 TTAA T^TAA
1307 TTAA
PflMI 975 CCAGTTGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1911 CCATGAACTGG
RsaI 1016 GTAC GT^AC
1614 GTAC
SapI 1271 GAAGAGC GCTCTTC(1/4)
267 GCTCTTC
Tru9I 1226 TTAA T^TAA
1307 TTAA
Van91I 975 CCAGTTGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1911 CCATGAACTGG
XhoII 878 GGATCT [GA]^GATC[CT]
901 GGATCT
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# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AciI AcsI AluI AlwI ApoI
AvaII BanI BbvI BmyI BpmI
BsaJI BseRI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrDI BsrFI BsrI
BssKI BstAPI BstF5I BstNI Cac8I
CfoI Cfr10I Cfr13I DpnII DsaI
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MaeIII MboI
MboII MnlI MslI MspA1I MspI
MvaI MwoI NdeII NlaIII NlaIV
Sau3AI Sau96I ScrFI SfaNI StyI
TaqI TfiI TseI Tsp45I Tsp509I
TspRI TthHB8I