Restriction map for
pF1KA0302
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# NO CUT ENZYMES ( 45 enzymes) #
##################################
AatII Acc65I AclI AflII AhdI
AscI AseI AsnI Asp718I AspEI
AspI BciVI BclI BfrI BspDI
BssHII Bst1107I BstZ17I ClaI CpoI
DraI Eam1105I EcoRI FbaI HpaI
KpnI MfeI MluI MunI NdeI
NheI NotI PacI PflFI PmeI
PshBI Psp1406I RsrII ScaI SgrAI
SnaBI SpeI SwaI Tth111I XbaI
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# ONE CUT ENZYMES ( 33 enzymes) #
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AccIII 5472 TCCGGA T^CCGGA
AcsI 5203 AAATTC [GA]^AATT[CT]
AgeI 2020 ACCGGT A^CCGGT
ApoI 5203 AAATTC [GA]^AATT[CT]
AvrII 2958 CCTAGG C^CTAGG
BbrPI 222 CACGTG CAC^GTG
BglII 5948 AGATCT A^GATCT
BlnI 2958 CCTAGG C^CTAGG
BsaAI 222 CACGTG [CT]AC^GT[GA]
BsaBI 4992 GATATCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 5472 TCCGGA T^CCGGA
BsiWI 5399 CGTACG C^GTACG
BsmBI 1411 GAGACG CGTCTC(1/5)
BspEI 5472 TCCGGA T^CCGGA
BspLU11I 6879 ACATGT A^CATGT
BstBI 2387 TTCGAA TT^CGAA
EcoRV 4992 GATATC GAT^ATC
EcoT22I 716 ATGCAT ATGCA^T
FseI 3278 GGCCGGCC GGCCGG^CC
MamI 4992 GATATCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 5472 TCCGGA T^CCGGA
NruI 4461 TCGCGA TCG^CGA
NsiI 716 ATGCAT ATGCA^T
NspV 2387 TTCGAA TT^CGAA
PinAI 2020 ACCGGT A^CCGGT
PmaCI 222 CACGTG CAC^GTG
PmlI 222 CACGTG CAC^GTG
PshAI 2931 GACCAAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 971 CGATCG CGAT^CG
SalI 6133 GTCGAC G^TCGAC
SfuI 2387 TTCGAA TT^CGAA
SphI 329 GCATGC GCATG^C
SspI 6914 AATATT AAT^ATT
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# TWO CUT ENZYMES ( 20 enzymes) #
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Alw44I 391 GTGCAC G^TGCAC
2898 GTGCAC
ApaLI 391 GTGCAC G^TGCAC
2898 GTGCAC
BbeI 4445 GGCGCC GGCGC^C
5981 GGCGCC
BsaWI 2020 ACCGGT [AT]^CCGG[AT]
5472 TCCGGA
Bsp1407I 242 TGTACA T^GTACA
591 TGTACA
BspHI 3650 TCATGA T^CATGA
4577 TCATGA
BsrGI 242 TGTACA T^GTACA
591 TGTACA
BstEII 2131 GGTCACC G^GT[ACGT]ACC
3390 GGTGACC
BstPI 2131 GGTCACC G^GT[ACGT]ACC
3390 GGTGACC
DrdI 2656 GACCTGGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2779 GACCGCATTGTC
EcoO65I 2131 GGTCACC G^GT[ACGT]ACC
3390 GGTGACC
HindIII 1123 AAGCTT A^AGCTT
6220 AAGCTT
KasI 4445 GGCGCC G^GCGCC
5981 GGCGCC
NarI 4445 GGCGCC GG^CGCC
5981 GGCGCC
RcaI 3650 TCATGA T^CATGA
4577 TCATGA
SapI 7012 GAAGAGC GCTCTTC(1/4)
1104 GCTCTTC
SexAI 4889 ACCAGGT A^CC[AT]GGT
6332 ACCTGGT
SfiI 3303 GGCCCTCCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
5307 GGCCACCGTGGCC
SfoI 4445 GGCGCC GGC^GCC
5981 GGCGCC
SspBI 242 TGTACA T^GTACA
591 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 160 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AflIII AluI AlwI AlwNI AocI
Aor51HI ApaI Asp700I AspHI AvaI
AvaII AviII BalI BamHI BanI
BanII BbsI BbvCI BbvI BcgI
BcnI BfaI BglI BlpI BmyI
BpmI Bpu1102I BpuAI BsaHI BsaI
BsaJI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BspMI BsrBI BsrDI
BsrFI BsrI BssKI BssSI BstAPI
BstF5I BstNI BstUI BstXI BstYI
Bsu36I Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DraIII
DsaI EaeI EagI EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
FspI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
KspI MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NciI NcoI
NdeII NgoMIV NlaIII NlaIV NspI
PaeR7I PflMI PleI PpuMI PstI
PvuII RsaI SacI SacII Sau3AI
Sau96I SbfI ScrFI SfaNI SfcI
SmaI SmlI Sse8387I StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoI XhoII XmaCI XmaI XmnI