Restriction map for
pF1KA0327
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# NO CUT ENZYMES ( 84 enzymes) #
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AatII Acc65I AclI AflII AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI Asp700I Asp718I AspEI AspI
AvrII BamHI BcgI BfrI BglI
BlnI BsaBI BsiWI Bsp1407I BspDI
BsrDI BsrGI Bst1107I BstBI BstZ17I
Bsu36I ClaI CpoI DraIII EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoRI EcoRV EcoT22I FseI HindIII
KpnI MamI MfeI MluI MunI
NaeI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PmeI PshAI Psp1406I
PvuI PvuII RsrII SapI SbfI
ScaI SfiI SfuI SnaBI SpeI
SphI Sse8387I SspBI StuI SwaI
Tth111I Van91I XhoI XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AccI 2044 GTCGAC GT^[AC][GT]AC
AccIII 842 TCCGGA T^CCGGA
AgeI 1531 ACCGGT A^CCGGT
AseI 850 ATTAAT AT^TAAT
AsnI 850 ATTAAT AT^TAAT
BalI 1947 TGGCCA TGG^CCA
BbsI 2107 GTCTTC GAAGAC(2/6)
BciVI 699 GGATAC GTATCC(6/5)
BlpI 513 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 513 GCTCAGC GC^T[ACGT]AGC
BpuAI 2107 GTCTTC GAAGAC(2/6)
BsaI 1577 GAGACC GGTCTC(1/5)
BseAI 842 TCCGGA T^CCGGA
BsiEI 2042 CGGTCG CG[GA][CT]^CG
BsmI 1449 GAATGC GAATGC(1/-1)
BspEI 842 TCCGGA T^CCGGA
BspHI 2391 TCATGA T^CATGA
BspLU11I 1634 ACATGT A^CATGT
BspMI 1580 ACCTGC ACCTGC(4/8)
BsrFI 1531 ACCGGT [GA]^CCGG[CT]
BstAPI 2086 GCAGTGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1761 GGTGACC G^GT[ACGT]ACC
BstPI 1761 GGTGACC G^GT[ACGT]ACC
CelII 513 GCTCAGC GC^T[ACGT]AGC
Cfr10I 1531 ACCGGT [GA]^CCGG[CT]
DraI 1174 TTTAAA TTT^AAA
DrdI 1528 GACACCGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 1761 GGTGACC G^GT[ACGT]ACC
HpaI 2373 GTTAAC GTT^AAC
MluNI 1947 TGGCCA TGG^CCA
MroI 842 TCCGGA T^CCGGA
MscI 1947 TGGCCA TGG^CCA
NcoI 1944 CCATGG C^CATGG
PinAI 1531 ACCGGT A^CCGGT
PshBI 850 ATTAAT AT^TAAT
RcaI 2391 TCATGA T^CATGA
SacI 280 GAGCTC GAGCT^C
SalI 2044 GTCGAC G^TCGAC
SgrAI 1530 CACCGGTG C[GA]^CCGG[CT]G
SmaI 1084 CCCGGG CCC^GGG
XcmI 2175 CCAGGATTCCGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 1084 CCCGGG C^CCGGG
XmaI 1084 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AcyI 1494 GGCGCC G[GA]^CG[CT]C
1731 GGCGCC
AfaI 1689 GTAC GT^AC
2236 GTAC
AflIII 1156 ACACGT A^C[GA][CT]GT
1634 ACATGT
AspHI 280 GAGCTC G[AT]GC[AT]^C
446 GAGCAC
AviII 677 TGCGCA TGC^GCA
1877 TGCGCA
BanI 1494 GGCGCC G^G[CT][GA]CC
1731 GGCGCC
BbeI 1494 GGCGCC GGCGC^C
1731 GGCGCC
BbvCI 1623 CCTCAGC CCTCAGC(-5/-2)
2145 GCTGAGG
BglII 408 AGATCT A^GATCT
1275 AGATCT
BsaHI 1494 GGCGCC G[GA]^CG[CT]C
1731 GGCGCC
BsiHKAI 280 GAGCTC G[AT]GC[AT]^C
446 GAGCAC
BsrBI 5 CCGCTC CCGCTC(-3/-3)
1737 CCGCTC
BssHII 50 GCGCGC G^CGCGC
591 GCGCGC
BssSI 1924 CTCGTG CACGAG(-5/-1)
2077 CTCGTG
DraII 149 AGGACCT [GA]G^G[ACGT]CC[CT]
1613 GGGACCC
EcoO109I 149 AGGACCT [GA]G^G[ACGT]CC[CT]
1613 GGGACCC
FspI 677 TGCGCA TGC^GCA
1877 TGCGCA
Hin1I 1494 GGCGCC G[GA]^CG[CT]C
1731 GGCGCC
KasI 1494 GGCGCC G^GCGCC
1731 GGCGCC
KspI 257 CCGCGG CCGC^GG
298 CCGCGG
NarI 1494 GGCGCC GG^CGCC
1731 GGCGCC
PpuMI 149 AGGACCT [GA]G^G[AT]CC[CT]
1613 GGGACCC
RsaI 1689 GTAC GT^AC
2236 GTAC
SacII 257 CCGCGG CCGC^GG
298 CCGCGG
SexAI 626 ACCTGGT A^CC[AT]GGT
1757 ACCTGGT
SfaNI 680 GCATC GCATC(5/9)
2000 GCATC
SfoI 1494 GGCGCC GGC^GCC
1731 GGCGCC
SspI 313 AATATT AAT^ATT
1223 AATATT
XbaI 411 TCTAGA T^CTAGA
918 TCTAGA
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# 3 OR MORE CUT ENZYMES ( 102 enzymes) #
#########################################
AccII AciI AcsI AluI AlwI
AlwNI ApoI AvaI AvaII BanII
BbrPI BbvI BclI BcnI BfaI
BmyI BpmI BsaAI BsaJI BsaWI
BseRI BsgI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I BsrI
BssKI BstF5I BstNI BstUI BstXI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DsaI EaeI EarI EcoNI
EcoRII EcoT14I FauI FbaI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PmaCI
PmlI PstI Sau3AI Sau96I ScrFI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII