Restriction map for
pF1KA0336
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# NO CUT ENZYMES ( 101 enzymes) #
##################################
AatII AccII AccIII AcyI AgeI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AspI AviII AvrII BalI
BamHI BanII BbeI BbrPI BbvCI
BcgI BciVI BlnI BsaAI BsaHI
BsaWI BseAI BsiEI BsmBI BsmFI
Bsp1407I BspDI BspEI BsrBI BsrFI
BsrGI BssHII BstEII BstPI BstUI
Bsu36I Cfr10I ClaI CpoI DraIII
DsaI EaeI EagI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRV
EcoT22I FseI FspI Hin1I KasI
KspI MluI MluNI MroI MscI
MvnI NaeI NarI NcoI NdeI
NgoMIV NotI NruI NsiI PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI PvuI RsrII
SacI SacII SalI SbfI ScaI
SfiI SfoI SgrAI SnaBI SpeI
Sse8387I SspBI StuI Tth111I Van91I
XhoI
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# ONE CUT ENZYMES ( 59 enzymes) #
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Acc65I 1356 GGTACC G^GTACC
AclI 3194 AACGTT AA^CGTT
AflIII 2359 ACATGT A^C[GA][CT]GT
AhdI 3978 GACTGTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 3410 CAGAAACTG CAG[ACGT][ACGT][ACGT]^CTG
Asp718I 1356 GGTACC G^GTACC
AspEI 3978 GACTGTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 3532 GAGCAC G[AT]GC[AT]^C
AvaI 4229 CCCGGG C^[CT]CG[GA]G
BanI 1356 GGTACC G^G[CT][GA]CC
BclI 3417 TGATCA T^GATCA
BglI 2617 GCCGTAAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 4638 GCTCAGC GC^T[ACGT]AGC
BmyI 3532 GAGCAC G[AGT]GC[ACT]^C
Bpu1102I 4638 GCTCAGC GC^T[ACGT]AGC
BsaBI 4712 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 2656 GAGACC GGTCTC(1/5)
BsgI 4409 GTGCAG GTGCAG(16/14)
BsiHKAI 3532 GAGCAC G[AT]GC[AT]^C
BsiWI 3565 CGTACG C^GTACG
BsoBI 4229 CCCGGG C^[CT]CG[GA]G
Bsp1286I 3532 GAGCAC G[AGT]GC[ACT]^C
BspLU11I 2359 ACATGT A^CATGT
BspMI 922 GCAGGT ACCTGC(4/8)
BssSI 3529 CACGAG CACGAG(-5/-1)
Bst1107I 1405 GTATAC GTA^TAC
BstAPI 4447 GCAACCAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 2981 CCAAAGCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 1405 GTATAC GTA^TAC
CelII 4638 GCTCAGC GC^T[ACGT]AGC
CfoI 3734 GCGC GCG^C
Eam1105I 3978 GACTGTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 3652 GAATTC G^AATTC
FauI 4203 CCCGC CCCGC(4/6)
FbaI 3417 TGATCA T^GATCA
HaeII 3733 GGCGCT [GA]GCGC^[CT]
HgaI 313 GACGC GACGC(5/10)
HhaI 3734 GCGC GCG^C
HinP1I 3734 GCGC G^CGC
HincII 3222 GTTAAC GT[CT]^[GA]AC
HindII 3222 GTTAAC GT[CT]^[GA]AC
HindIII 4477 AAGCTT A^AGCTT
HpaI 3222 GTTAAC GTT^AAC
KpnI 1356 GGTACC GGTAC^C
MamI 4712 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 3364 CAATTG C^AATTG
MunI 3364 CAATTG C^AATTG
NheI 918 GCTAGC G^CTAGC
NlaIV 1356 GGTACC GG[ACGT]^[ACGT]CC
PpuMI 4007 AGGTCCT [GA]G^G[AT]CC[CT]
Psp1406I 3194 AACGTT AA^CGTT
SexAI 4587 ACCAGGT A^CC[AT]GGT
SmaI 4229 CCCGGG CCC^GGG
SphI 4353 GCATGC GCATG^C
SspI 394 AATATT AAT^ATT
SwaI 1916 ATTTAAAT ATTT^AAAT
Tsp45I 3592 GTGAC ^GT[GC]AC
XmaCI 4229 CCCGGG C^CCGGG
XmaI 4229 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccI 1405 GTATAC GT^[AC][GT]AC
2992 GTAGAC
AvaII 2703 GGACC G^G[AT]CC
4008 GGTCC
BglII 2727 AGATCT A^GATCT
2904 AGATCT
BpmI 1315 CTCCAG CTGGAG(16/14)
3454 CTGGAG
BsmI 2292 GAATGC GAATGC(1/-1)
2572 GAATGC
BspHI 828 TCATGA T^CATGA
1245 TCATGA
BstBI 1210 TTCGAA TT^CGAA
4013 TTCGAA
BstYI 2727 AGATCT [GA]^GATC[CT]
2904 AGATCT
DraII 2261 AGGCCCT [GA]G^G[ACGT]CC[CT]
4007 AGGTCCT
DrdI 1023 GACATTTTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2925 GACATTACAGTC
EcoO109I 2261 AGGCCCT [GA]G^G[ACGT]CC[CT]
4007 AGGTCCT
EcoT14I 2063 CCTTGG C^C[AT][AT]GG
3623 CCAAGG
HapII 3918 CCGG C^CGG
4230 CCGG
HpaII 3918 CCGG C^CGG
4230 CCGG
MflI 2727 AGATCT [GA]^GATC[CT]
2904 AGATCT
MslI 3162 CACATTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
3951 CATGATGATG
MspI 3918 CCGG C^CGG
4230 CCGG
NspV 1210 TTCGAA TT^CGAA
4013 TTCGAA
RcaI 828 TCATGA T^CATGA
1245 TCATGA
SfuI 1210 TTCGAA TT^CGAA
4013 TTCGAA
StyI 2063 CCTTGG C^C[AT][AT]GG
3623 CCAAGG
XbaI 2280 TCTAGA T^CTAGA
4209 TCTAGA
XcmI 3380 CCAAAAAGGAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4367 CCAAAGAAGAATTGG
XhoII 2727 AGATCT [GA]^GATC[CT]
2904 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 74 enzymes) #
#########################################
AciI AcsI AfaI AflII AluI
AlwI ApoI AseI AsnI Asp700I
BbsI BbvI BcnI BfaI BfrI
BpuAI BsaJI BseRI BsiYI BslI
BsmAI BsrDI BsrI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DpnII
DraI EarI EcoRII Fnu4HI FokI
HaeIII HinfI HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MnlI MseI MspA1I MvaI MwoI
NciI NdeII NlaIII NspI PleI
PshBI PstI PvuII RsaI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI TaqI TfiI Tru9I TseI
Tsp509I TspRI TthHB8I XmnI