Restriction map for
pF1KA0363
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# NO CUT ENZYMES ( 89 enzymes) #
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AatII AccI AccIII AclI AcsI
AflII AflIII AgeI ApoI AscI
AseI AsnI AviII AvrII BbeI
BbrPI BciVI BclI BfrI BlnI
BsaAI BsaWI BseAI BsiWI BspDI
BspEI BspHI BspLU11I BssHII BssSI
Bst1107I BstBI BstEII BstPI BstZ17I
ClaI CpoI DraI DrdI EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HindIII HpaI KasI KspI
MfeI MluI MroI MunI NarI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SfoI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspI SwaI XbaI XhoI
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# ONE CUT ENZYMES ( 54 enzymes) #
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Acc65I 3418 GGTACC G^GTACC
AcyI 307 GACGCC G[GA]^CG[CT]C
AhdI 784 GACAGCACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 4147 GTGCAC G^TGCAC
Aor51HI 132 AGCGCT AGC^GCT
ApaLI 4147 GTGCAC G^TGCAC
Asp700I 1351 GAAAAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 3418 GGTACC G^GTACC
AspEI 784 GACAGCACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 4147 GTGCAC G[AT]GC[AT]^C
BamHI 741 GGATCC G^GATCC
BfaI 2858 CTAG C^TAG
BsaBI 4023 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 307 GACGCC G[GA]^CG[CT]C
BsiEI 386 CGGCCG CG[GA][CT]^CG
BsiHKAI 4147 GTGCAC G[AT]GC[AT]^C
BsmBI 91 GAGACG CGTCTC(1/5)
Bsp1407I 932 TGTACA T^GTACA
BspMI 1185 GCAGGT ACCTGC(4/8)
BsrBI 999 GAGCGG CCGCTC(-3/-3)
BsrFI 2817 GCCGGC [GA]^CCGG[CT]
BsrGI 932 TGTACA T^GTACA
Cfr10I 2817 GCCGGC [GA]^CCGG[CT]
DraIII 1386 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 386 CGGCCG C^GGCCG
Eam1105I 784 GACAGCACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 386 CGGCCG C^GGCCG
Eco47III 132 AGCGCT AGC^GCT
Eco52I 386 CGGCCG C^GGCCG
HaeII 132 AGCGCT [GA]GCGC^[CT]
Hin1I 307 GACGCC G[GA]^CG[CT]C
HincII 4381 GTCAAC GT[CT]^[GA]AC
HindII 4381 GTCAAC GT[CT]^[GA]AC
KpnI 3418 GGTACC GGTAC^C
MaeI 2858 CTAG C^TAG
MamI 4023 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI 4172 TTAA T^TAA
NaeI 2817 GCCGGC GCC^GGC
NgoMIV 2817 GCCGGC G^CCGGC
NspI 2875 GCATGT [GA]CATG^[CT]
PflMI 1231 CCACAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 2996 GACCCAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SexAI 2708 ACCAGGT A^CC[AT]GGT
SmaI 4223 CCCGGG CCC^GGG
SmlI 3740 CTCAAG C^T[CT][GA]AG
SspBI 932 TGTACA T^GTACA
TaqI 605 TCGA T^CGA
Tru9I 4172 TTAA T^TAA
Tsp509I 1109 AATT ^AATT
TthHB8I 605 TCGA T^CGA
Van91I 1231 CCACAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 4223 CCCGGG C^CCGGG
XmaI 4223 CCCGGG C^CCGGG
XmnI 1351 GAAAAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 8 enzymes) #
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BcgI 846 CGAGGCTTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
867 CGACACCTCTGC
BsaI 2935 GAGACC GGTCTC(1/5)
3928 GAGACC
BsmI 2667 GAATGC GAATGC(1/-1)
3822 GCATTC
BsrDI 1288 GCAATG GCAATG(2/0)
3979 GCAATG
BstAPI 2575 GCAGGCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3681 GCAGATCTTGC
BstXI 144 CCAGGAGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3665 CCAAAGACCTGG
HgaI 307 GACGC GACGC(5/10)
3226 GACGC
SfiI 586 GGCCAGGAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
3150 GGCCACAGAGGCC
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# 3 OR MORE CUT ENZYMES ( 107 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AocI ApaI AspI AvaI
AvaII BalI BanI BanII BbsI
BbvCI BbvI BcnI BglI BglII
BlpI BmyI BpmI Bpu1102I BpuAI
BsaJI BseRI BsgI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrI
BssKI BstF5I BstNI BstUI BstYI
Bsu36I Cac8I CelII CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MslI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV PflFI PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StuI StyI
TfiI TseI Tsp45I TspRI Tth111I
XcmI XhoII