Restriction map for
pF1KA0394
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# NO CUT ENZYMES ( 127 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AhdI Alw44I Aor51HI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AviII
AvrII BalI BanI BbeI BbrPI
BbvCI BclI BfrI BglI BglII
BlnI BsaAI BsaHI BseAI BsgI
BsiEI BsiWI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BspMI BsrGI
BssHII BssSI Bst1107I BstAPI BstZ17I
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoRV EcoT22I FauI FbaI
FseI FspI HaeII HgaI Hin1I
HpaI KasI KpnI KspI MfeI
MluI MluNI MroI MscI MseI
MunI NarI NcoI NdeI NheI
NotI NruI NsiI PacI PaeR7I
PflFI PleI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsrII SacII SalI SbfI
ScaI SexAI SfcI SfiI SfoI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI Tru9I
Tth111I XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AccII 923 CGCG CG^CG
AcsI 523 GAATTC [GA]^AATT[CT]
AflIII 1109 ACATGT A^C[GA][CT]GT
AgeI 265 ACCGGT A^CCGGT
AocI 73 CCTCAGG CC^T[ACGT]AGG
ApaI 632 GGGCCC GGGCC^C
ApoI 523 GAATTC [GA]^AATT[CT]
BamHI 286 GGATCC G^GATCC
BcgI 785 GCAAGCAGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 199 GGATAC GTATCC(6/5)
BfaI 1030 CTAG C^TAG
BsaBI 287 GATCCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 265 ACCGGT [AT]^CCGG[AT]
BseRI 904 GAGGAG GAGGAG(10/8)
BsmBI 947 GAGACG CGTCTC(1/5)
BspLU11I 1109 ACATGT A^CATGT
BsrDI 771 CATTGC GCAATG(2/0)
BstBI 1151 TTCGAA TT^CGAA
BstEII 1011 GGTGACC G^GT[ACGT]ACC
BstPI 1011 GGTGACC G^GT[ACGT]ACC
BstUI 923 CGCG CG^CG
Bsu36I 73 CCTCAGG CC^T[ACGT]AGG
DsaI 1136 CCGTGG C^C[GA][CT]GG
EaeI 1166 CGGCCA [CT]^GGCC[GA]
Eco81I 73 CCTCAGG CC^T[ACGT]AGG
EcoO65I 1011 GGTGACC G^GT[ACGT]ACC
EcoRI 523 GAATTC G^AATTC
HincII 94 GTCAAC GT[CT]^[GA]AC
HindII 94 GTCAAC GT[CT]^[GA]AC
HindIII 691 AAGCTT A^AGCTT
MaeI 1030 CTAG C^TAG
MamI 287 GATCCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MslI 244 CACATGAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 923 CGCG CG^CG
NaeI 80 GCCGGC GCC^GGC
NgoMIV 80 GCCGGC G^CCGGC
NspI 1109 ACATGT [GA]CATG^[CT]
NspV 1151 TTCGAA TT^CGAA
PinAI 265 ACCGGT A^CCGGT
SacI 174 GAGCTC GAGCT^C
SapI 645 GAAGAGC GCTCTTC(1/4)
SfuI 1151 TTCGAA TT^CGAA
SgrAI 264 CACCGGTG C[GA]^CCGG[CT]G
SmlI 676 CTCAAG C^T[CT][GA]AG
Tsp509I 524 AATT ^AATT
TspRI 191 CAGTG CA[GC]TG[ACGT][ACGT]^
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AfaI 87 GTAC GT^AC
1083 GTAC
AspHI 174 GAGCTC G[AT]GC[AT]^C
1195 GAGCAC
BbsI 553 GAAGAC GAAGAC(2/6)
864 GAAGAC
BlpI 574 GCTAAGC GC^T[ACGT]AGC
888 GCTGAGC
Bpu1102I 574 GCTAAGC GC^T[ACGT]AGC
888 GCTGAGC
BpuAI 553 GAAGAC GAAGAC(2/6)
864 GAAGAC
BsaI 112 GAGACC GGTCTC(1/5)
851 GAGACC
BsiHKAI 174 GAGCTC G[AT]GC[AT]^C
1195 GAGCAC
BsrBI 841 GAGCGG CCGCTC(-3/-3)
1033 GAGCGG
BsrFI 80 GCCGGC [GA]^CCGG[CT]
265 ACCGGT
BsrI 987 CCAGT ACTGG(1/-1)
1080 CCAGT
BstYI 286 GGATCC [GA]^GATC[CT]
969 GGATCT
CelII 574 GCTAAGC GC^T[ACGT]AGC
888 GCTGAGC
Cfr10I 80 GCCGGC [GA]^CCGG[CT]
265 ACCGGT
DraII 214 GGGACCC [GA]G^G[ACGT]CC[CT]
434 AGGACCC
EarI 645 GAAGAG CTCTTC(1/4)
1003 GAAGAG
EcoO109I 214 GGGACCC [GA]G^G[ACGT]CC[CT]
434 AGGACCC
HinfI 147 GATTC G^A[ACGT]TC
271 GATTC
Ksp632I 645 GAAGAG CTCTTC(1/4)
1003 GAAGAG
MaeII 126 ACGT A^CGT
346 ACGT
MaeIII 343 GTGAC ^GT[ACGT]AC
1012 GTGAC
MflI 286 GGATCC [GA]^GATC[CT]
969 GGATCT
PflMI 276 CCAGAACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
870 CCAGCAGCTGG
PpuMI 214 GGGACCC [GA]G^G[AT]CC[CT]
434 AGGACCC
RsaI 87 GTAC GT^AC
1083 GTAC
SfaNI 366 GATGC GCATC(5/9)
208 GCATC
TaqI 1130 TCGA T^CGA
1152 TCGA
TfiI 147 GATTC G^A[AT]TC
271 GATTC
Tsp45I 343 GTGAC ^GT[GC]AC
1012 GTGAC
TthHB8I 1130 TCGA T^CGA
1152 TCGA
Van91I 276 CCAGAACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
870 CCAGCAGCTGG
XhoII 286 GGATCC [GA]^GATC[CT]
969 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 53 enzymes) #
#########################################
AciI AluI AlwI AlwNI AvaII
BanII BbvI BcnI BmyI BpmI
BsaJI BsiYI BslI BsmAI BsmFI
Bsp1286I BssKI BstF5I BstNI BstXI
Cac8I CfoI Cfr13I DdeI DpnII
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HhaI HinP1I HpaII HphI
ItaI MboI MboII MnlI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PvuII Sau3AI Sau96I
ScrFI StyI TseI